Restriction map for
pFN21AA0750
(Circular) (Six-base) MAPSORT of: pFN21AA0750.seq Check: 4351 from: 1 to: 8077
pFN21AA0750 8077 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2658 5887 278
Size: 53 83 186 936 1122 3229 2468
Fragments arranged by size:
3229 2468 1122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5420 1937
Size: 3483 4594
Acc65I G'GTAC_C
Cuts at: 2084 3414 5398 2084
Size: 1330 1984 4763
Fragments arranged by size:
4763 1984 1330
AclI AA'CG_TT
Cuts at: 1563 6204 6577 1563
Size: 4641 373 3063
Fragments arranged by size:
4641 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2595 4788 4914 6140 7524 7792 1632
Size: 963 2193 126 1226 1384 268 1917
Fragments arranged by size:
2193 1917 1384 1226 963 268 126
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7880
AflIII A'CryG_T
Cuts at: 1203 3393 5004 5006 5026 6976 7953 1203
Size: 2190 1611 2 20 1950 977 1327
Fragments arranged by size:
2190 1950 1611 1327 977 20 2
AgeI A'CCGG_T
Cuts at: 6908 6908
Size: 8077
AhdI GACnn_n'nnGTC
Cuts at: 6806 6806
Size: 8077
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8077
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2092 4410 1184
Size: 908 2318 4851
Fragments arranged by size:
4851 2318 908
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4828 4860 4828
Size: 32 8045
AlwNI CAG_nnn'CTG
Cuts at: 2926 4171 5097 5820 7392 2926
Size: 1245 926 723 1572 3611
Fragments arranged by size:
3611 1572 1245 926 723
ApaLI G'TGCA_C
Cuts at: 2254 6134 7290 2254
Size: 3880 1156 3041
Fragments arranged by size:
3880 3041 1156
ApoI r'AATT_y
Cuts at: 1493 2612 2711 2822 4468 4589 5150 5386 5612
Size: 1119 99 111 1646 121 561 236 226
Cuts at: 5612 5764 7873 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1646 1119 561 236 226 152
121 111 99
AseI AT'TA_AT
Cuts at: 160 1032 6631 160
Size: 872 5599 1606
Fragments arranged by size:
5599 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8077
AvaI C'yCGr_G
Cuts at: 1870 1943 3021 3497 4495 5402 1870
Size: 73 1078 476 998 907 4545
Fragments arranged by size:
4545 1078 998 907 476 73
BamHI G'GATC_C
Cuts at: 3771 5407 5774 3771
Size: 1636 367 6074
Fragments arranged by size:
6074 1636 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2666 3296 3414 3579
Size: 325 217 627 297 582 630 118 165
Cuts at: 3579 3872 5315 5398 6853 618
Size: 293 1443 83 1455 1842
Fragments arranged by size:
1842 1455 1443 630 627 582 325 297
293 217 165 118 83
BanII G_rGCy'C
Cuts at: 1391 3512 3690 3864 4100 4383 4570 5396 1391
Size: 2121 178 174 236 283 187 826 4072
Fragments arranged by size:
4072 2121 826 283 236 187 178 174
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7106
BbvCI CC'TCA_GC
Cuts at: 3929 4848 3929
Size: 919 7158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 6268 6302 1903
Size: 34 2213 34 2084 34 3678
Fragments arranged by size:
3678 2213 2084 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5969 7179 5969
Size: 1210 6867
BclI T'GATC_A
Cuts at: 1548 4921 1548
Size: 3373 4704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3217 6688 136
Size: 107 122 71 631 605 1545 3471 1525
Fragments arranged by size:
3471 1545 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2857 3521 8072 2857
Size: 664 4551 2862
Fragments arranged by size:
4551 2862 664
BlpI GC'TnA_GC
Cuts at: 3827 5048 5494 3827
Size: 1221 446 6410
Fragments arranged by size:
6410 1221 446
Bme1580I G_kGCm'C
Cuts at: 1790 2258 3241 3299 3875 4368 6138 7294 1790
Size: 468 983 58 576 493 1770 1156 2573
Fragments arranged by size:
2573 1770 1156 983 576 493 468 58
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8077
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3515 5330 5808
Size: 416 53 83 399 24 2098 1815 478
Cuts at: 5808 6766 442
Size: 958 1753
Fragments arranged by size:
2098 1815 1753 958 478 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8077
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5295 5295
Size: 8077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 3329 3503 3520 3762 4541 4935 6737
Size: 836 1254 174 17 242 779 394 1802
Cuts at: 6737 1239
Size: 2579
Fragments arranged by size:
2579 1802 1254 836 779 394 242 174
17
Bpu10I CC'TnA_GC
Cuts at: 3684 3929 4412 4848 3684
Size: 245 483 436 6913
Fragments arranged by size:
6913 483 436 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 2882 3227 4367 6202 7067 7365 2147
Size: 735 345 1140 1835 865 298 2859
Fragments arranged by size:
2859 1835 1140 865 735 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6740 882
Size: 617 5241 2219
Fragments arranged by size:
5241 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2076 4369 5007 5035 5868 493
Size: 711 872 2293 638 28 833 2702
Fragments arranged by size:
2702 2293 872 833 711 638 28
BsaBI GATnn'nnATC
Cuts at: 2735 8071 2735
Size: 5336 2741
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2655 5884 6266 6901
Size: 53 83 186 936 1122 3229 382 635
Cuts at: 6901 275
Size: 1451
Fragments arranged by size:
3229 1451 1122 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4013 6510 6908 7182 7329 7740 4013
Size: 2497 398 274 147 411 4350
Fragments arranged by size:
4350 2497 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2322 2351 2352 2381 2633
Size: 30 165 30 919 29 1 29 252
Cuts at: 2633 2663 4616 4646 4684 4714 1178
Size: 30 1953 30 38 30 4541
Fragments arranged by size:
4541 1953 919 252 165 38 30 30
30 30 30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3532 4199 4316 4317 4698 5249 5252 1186
Size: 2346 667 117 1 381 551 3 4011
Fragments arranged by size:
4011 2346 667 551 381 117 3 1
BseYI C'CCAG_C
Cuts at: 2521 3560 4313 4453 4823 7280 7776 2521
Size: 1039 753 140 370 2457 496 2822
Fragments arranged by size:
2822 2457 1039 753 496 370 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2872 1163
Size: 573 1136 6368
Fragments arranged by size:
6368 1136 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5527 6288 7316 1540
Size: 275 186 3526 761 1028 2301
Fragments arranged by size:
3526 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2258 3489 3690 3864 4193 4259 4374 4383 6138
Size: 1231 201 174 329 66 115 9 1755
Cuts at: 6138 6223 7294 2258
Size: 85 1071 3041
Fragments arranged by size:
3041 1755 1231 1071 329 201 174 115
85 66 9
BsmI GAATG_Cn'
Cuts at: 5588 5681 5588
Size: 93 7984
BsmBI CGTCTCn'nnnn_
Cuts at: 4033 4731 5200 4033
Size: 698 469 6910
Fragments arranged by size:
6910 698 469
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2258 3241 3299 3489 3512 3690 3864
Size: 399 468 983 58 190 23 178 174
Cuts at: 3864 3875 4100 4193 4259 4368 4374 4383 4570
Size: 11 225 93 66 109 6 9 187
Cuts at: 4570 5396 6138 6223 7294 1391
Size: 826 742 85 1071 2174
Fragments arranged by size:
2174 1071 983 826 742 468 399 225
190 187 178 174 109 93 85 66
58 23 11 9 6
BspEI T'CCGG_A
Cuts at: 7740 7740
Size: 8077
BspHI T'CATG_A
Cuts at: 4209 5966 7696 4209
Size: 1757 1730 4590
Fragments arranged by size:
4590 1757 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2035 3015 3128 5432 844
Size: 1191 980 113 2304 3489
Fragments arranged by size:
3489 2304 1191 980 113
BsrBI CCG'CTC
Cuts at: 1394 1823 3160 5964 1394
Size: 429 1337 2804 3507
Fragments arranged by size:
3507 2804 1337 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6572 6746 65
Size: 1183 5324 174 1396
Fragments arranged by size:
5324 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3339 4246 6721 6908 1700
Size: 110 1529 907 2475 187 2869
Fragments arranged by size:
2869 2475 1529 907 187 110
BsrGI T'GTAC_A
Cuts at: 96 4911 96
Size: 4815 3262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8077
BssSI C'ACGA_G
Cuts at: 3179 6137 7149 3179
Size: 2958 1012 4107
Fragments arranged by size:
4107 2958 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2926 3038 3175 4407 794
Size: 2132 112 137 1232 4464
Fragments arranged by size:
4464 2132 1232 137 112
BstBI TT'CG_AA
Cuts at: 6881 6881
Size: 8077
BstEII G'GTnAC_C
Cuts at: 1184 2173 1184
Size: 989 7088
BstYI r'GATC_y
Cuts at: 1972 2857 3521 3771 5407 5774 6159 6176 7628
Size: 885 664 250 1636 367 385 17 1452
Cuts at: 7628 7714 7726 8072 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1636 1452 885 664 385 367 346
250 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4932 4932
Size: 8077
BtgI C'CryG_G
Cuts at: 513 1065 2004 2716 3222 3241 3786 3888 3995
Size: 552 939 712 506 19 545 102 107
Cuts at: 3995 4155 513
Size: 160 4435
Fragments arranged by size:
4435 939 712 552 545 506 160 107
102 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5154 487
Size: 683 56 3928 3410
Fragments arranged by size:
3928 3410 683 56
BtsI GCAGTG_nn'
Cuts at: 2304 3649 4408 5307 5593 6387 6407 2304
Size: 1345 759 899 286 794 20 3974
Fragments arranged by size:
3974 1345 899 794 759 286 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8042
DraI TTT'AAA
Cuts at: 5381 5381
Size: 8077
DraIII CAC_nnn'GTG
Cuts at: 2268 3437 2268
Size: 1169 6908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2939 7084 809
Size: 507 1623 4145 1802
Fragments arranged by size:
4145 1802 1623 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2674 3102 3753
Size: 54 1425 50 275 18 844 428 651
Cuts at: 3753 3936 4173 4578 5118 5304 5524 6413 8042
Size: 183 237 405 540 186 220 889 1629
Cuts at: 8042 8
Size: 43
Fragments arranged by size:
1629 1425 889 844 651 540 428 405
275 237 220 186 183 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5524 1537
Size: 275 3712 4090
Fragments arranged by size:
4090 3712 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2348 2781 3275 3869 3980 4264 4556 5198
Size: 995 433 494 594 111 284 292 642
Cuts at: 5198 6007 1353
Size: 809 3423
Fragments arranged by size:
3423 995 809 642 594 494 433 292
284 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6664 7038 7184 387
Size: 1064 138 5075 374 146 1280
Fragments arranged by size:
5075 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3688 3862 4381 3688
Size: 174 519 7384
Fragments arranged by size:
7384 519 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2595 3329 3503 3520 3762 4541
Size: 393 443 520 734 174 17 242 779
Cuts at: 4541 4788 4914 4935 6140 6737 7524 7792 1239
Size: 247 126 21 1205 597 787 268 1524
Fragments arranged by size:
1524 1205 787 779 734 597 520 443
393 268 247 242 174 126 21 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 2504
Size: 8077
EcoO109I rG'GnC_Cy
Cuts at: 2288 2419 2833 3444 2288
Size: 131 414 611 6921
Fragments arranged by size:
6921 611 414 131
EcoRI G'AATT_C
Cuts at: 2711 5386 5764 2711
Size: 2675 378 5024
Fragments arranged by size:
5024 2675 378
EcoRV GAT'ATC
Cuts at: 3553 3553
Size: 8077
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5185 5185
Size: 8077
FspI TGC'GCA
Cuts at: 2303 5299 6583 2303
Size: 2996 1284 3797
Fragments arranged by size:
3797 2996 1284
HaeII r_GCGC'y
Cuts at: 1678 5055 7224 1678
Size: 3377 2169 2531
Fragments arranged by size:
3377 2531 2169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 2517 2549 3788
Size: 32 135 32 779 32 134 32 1239
Cuts at: 3788 3820 4946 4978 6718 6750 6792 6824 1373
Size: 32 1126 32 1740 32 42 32 2626
Fragments arranged by size:
2626 1740 1239 1126 779 135 134 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3529 3613 4765 5421 5667 669
Size: 988 281 1591 84 1152 656 246 3079
Fragments arranged by size:
3079 1591 1152 988 656 281 246 84
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8077
HpaI GTT'AAC
Cuts at: 5667 5667
Size: 8077
KpnI G_GTAC'C
Cuts at: 2088 3418 5402 2088
Size: 1330 1984 4763
Fragments arranged by size:
4763 1984 1330
MfeI C'AATT_G
Cuts at: 5676 5676
Size: 8077
MluI A'CGCG_T
Cuts at: 7953 7953
Size: 8077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2619 3378 7191 7375 1305
Size: 1314 759 3813 184 2007
Fragments arranged by size:
3813 2007 1314 759 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3104 3938 4175 4580 10
Size: 54 1425 343 1272 834 237 405 3507
Fragments arranged by size:
3507 1425 1272 834 405 343 237 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2074 2541 2969 3836 6035 6394 6553
Size: 648 908 467 428 867 2199 359 159
Cuts at: 6553 518
Size: 2042
Fragments arranged by size:
2199 2042 908 867 648 467 428 359
159
MspA1I CmG'CkG
Cuts at: 1606 2771 3055 3175 4168 4453 4739 4852 4875
Size: 1165 284 120 993 285 286 113 23
Cuts at: 4875 4892 5088 5493 6170 7318 7563 1606
Size: 17 196 405 677 1148 245 2120
Fragments arranged by size:
2120 1165 1148 993 677 405 286 285
284 245 196 120 113 23 17
NcoI C'CATG_G
Cuts at: 513 1065 2004 3222 4155 513
Size: 552 939 1218 933 4435
Fragments arranged by size:
4435 1218 939 933 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8077
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8077
NotI GC'GGCC_GC
Cuts at: 5524 5524
Size: 8077
NspI r_CATG'y
Cuts at: 1132 1229 3127 3611 5435 6980 1132
Size: 97 1898 484 1824 1545 2229
Fragments arranged by size:
2229 1898 1824 1545 484 97
PasI CC'CwG_GG
Cuts at: 1348 3404 1348
Size: 2056 6021
PciI A'CATG_T
Cuts at: 6976 6976
Size: 8077
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3636 5372 1108
Size: 2528 1736 3813
Fragments arranged by size:
3813 2528 1736
PfoI T'CCnGG_A
Cuts at: 2369 3536 2369
Size: 1167 6910
PmeI GTTT'AAAC
Cuts at: 5381 5381
Size: 8077
PmlI CAC'GTG
Cuts at: 2076 4369 5007 2076
Size: 2293 638 5146
Fragments arranged by size:
5146 2293 638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6146 6178 7659 7691 6146
Size: 32 1481 32 6532
Fragments arranged by size:
6532 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2288 2833 2288
Size: 545 7532
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8077
PsiI TTA'TAA
Cuts at: 5647 5647
Size: 8077
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 8045
PstI C_TGCA'G
Cuts at: 830 3034 4536 4891 5093 5429 830
Size: 2204 1502 355 202 336 3478
Fragments arranged by size:
3478 2204 1502 355 336 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8077
PvuII CAG'CTG
Cuts at: 3055 3175 4168 4453 4739 4852 4892 3055
Size: 120 993 285 286 113 40 6240
Fragments arranged by size:
6240 993 286 285 120 113 40
SacI G_AGCT'C
Cuts at: 3690 3864 4383 3690
Size: 174 519 7384
Fragments arranged by size:
7384 519 174
SalI G'TCGA_C
Cuts at: 1936 5419 1936
Size: 3483 4594
SapI GCTCTTCn'nnn_
Cuts at: 3275 3869 4264 3275
Size: 594 395 7088
Fragments arranged by size:
7088 594 395
SbfI CC_TGCA'GG
Cuts at: 5429 5429
Size: 8077
ScaI AGT'ACT
Cuts at: 6325 8037 6325
Size: 1712 6365
SexAI A'CCwGG_T
Cuts at: 3575 3575
Size: 8077
SfcI C'TryA_G
Cuts at: 826 1045 2489 2622 3030 4448 4532 4887 5089
Size: 219 1444 133 408 1418 84 355 202
Cuts at: 5089 5425 6560 7241 7432 826
Size: 336 1135 681 191 1471
Fragments arranged by size:
1471 1444 1418 1135 681 408 355 336
219 202 191 133 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8077
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8077
SmaI CCC'GGG
Cuts at: 1872 3023 5404 1872
Size: 1151 2381 4545
Fragments arranged by size:
4545 2381 1151
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 2861 3206 3497 4382 6181
Size: 197 926 219 699 345 291 885 1799
Cuts at: 6181 7082 7344 820
Size: 901 262 1553
Fragments arranged by size:
1799 1553 926 901 885 699 345 291
262 219 197
SnaBI TAC'GTA
Cuts at: 493 5868 493
Size: 5375 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8077
SphI G_CATG'C
Cuts at: 1132 5435 1132
Size: 4303 3774
SspI AAT'ATT
Cuts at: 5 52 6001 5
Size: 47 5949 2081
Fragments arranged by size:
5949 2081 47
StuI AGG'CCT
Cuts at: 1517 2096 4683 1517
Size: 579 2587 4911
Fragments arranged by size:
4911 2587 579
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 2778 3222 3980 4155 4421
Size: 552 939 165 609 444 758 175 266
Cuts at: 4421 5516 513
Size: 1095 3074
Fragments arranged by size:
3074 1095 939 758 609 552 444 266
175 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2906 3182 4327 4445 5379 6104 6121
Size: 1255 1326 276 1145 118 934 725 17
Cuts at: 6121 6274 6459 6904 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1326 1255 1145 934 725 445 276
185 153 118 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3390 4911 6323
Size: 274 80 33 51 1443 1413 1521 1412
Cuts at: 6323 8035 96
Size: 1712 138
Fragments arranged by size:
1712 1521 1443 1413 1412 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2322 2354 2437 2469 4616 4648 8037 8069 2322
Size: 32 83 32 2147 32 3389 32 2330
Fragments arranged by size:
3389 2330 2147 83 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8077
XbaI T'CTAG_A
Cuts at: 2563 5413 2563
Size: 2850 5227
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 4090 5113 5317 2341
Size: 1749 1023 204 5101
Fragments arranged by size:
5101 1749 1023 204
XhoI C'TCGA_G
Cuts at: 1943 3497 1943
Size: 1554 6523
XmaI C'CCGG_G
Cuts at: 1870 3021 5402 1870
Size: 1151 2381 4545
Fragments arranged by size:
4545 2381 1151
XmnI GAAnn'nnTTC
Cuts at: 4049 4551 6206 4049
Size: 502 1655 5920
Fragments arranged by size:
5920 1655 502
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2656 5885 276
Size: 53 83 186 936 1122 3229 2468
Fragments arranged by size:
3229 2468 1122 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacII
SanDI SfiI SfoI SrfI SwaI