(Circular) (Six-base) MAPSORT of: pFN21AA0739.seq Check: 611 from: 1 to: 7918 pFN21AA0739 7918 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3984 3984 Size: 7918 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5728 278 Size: 53 83 186 936 4192 2468 Fragments arranged by size: 4192 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2943 5190 5261 1937 Size: 1006 2247 71 4594 Fragments arranged by size: 4594 2247 1006 71 Acc65I G'GTAC_C Cuts at: 5239 5239 Size: 7918 AclI AA'CG_TT Cuts at: 1563 6045 6418 1563 Size: 4482 373 3063 Fragments arranged by size: 4482 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2525 3404 3885 5981 7365 7633 1632 Size: 893 879 481 2096 1384 268 1917 Fragments arranged by size: 2096 1917 1384 893 879 481 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7721 AflIII A'CryG_T Cuts at: 1203 4642 6817 7794 1203 Size: 3439 2175 977 1327 Fragments arranged by size: 3439 2175 1327 977 AgeI A'CCGG_T Cuts at: 6749 6749 Size: 7918 AhdI GACnn_n'nnGTC Cuts at: 6647 6647 Size: 7918 AleI CACnn'nnGTG Cuts at: 1166 4307 4643 1166 Size: 3141 336 4441 Fragments arranged by size: 4441 3141 336 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7918 AlwNI CAG_nnn'CTG Cuts at: 5661 7233 5661 Size: 1572 6346 ApaLI G'TGCA_C Cuts at: 4940 5975 7131 4940 Size: 1035 1156 5727 Fragments arranged by size: 5727 1156 1035 ApoI r'AATT_y Cuts at: 1493 2652 2964 3734 4848 5227 5453 5605 7714 Size: 1159 312 770 1114 379 226 152 2109 Cuts at: 7714 1493 Size: 1697 Fragments arranged by size: 2109 1697 1159 1114 770 379 312 226 152 AseI AT'TA_AT Cuts at: 160 1032 2607 4401 6472 160 Size: 872 1575 1794 2071 1606 Fragments arranged by size: 2071 1794 1606 1575 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7918 AvaI C'yCGr_G Cuts at: 1870 1943 5243 1870 Size: 73 3300 4545 Fragments arranged by size: 4545 3300 73 BamHI G'GATC_C Cuts at: 3289 5248 5615 3289 Size: 1959 367 5592 Fragments arranged by size: 5592 1959 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2396 5239 6694 618 Size: 325 217 627 609 2843 1455 1842 Fragments arranged by size: 2843 1842 1455 627 609 325 217 BanII G_rGCy'C Cuts at: 1391 5237 1391 Size: 3846 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4756 928 Size: 971 2857 4090 Fragments arranged by size: 4090 2857 971 BbvCI CC'TCA_GC Cuts at: 2802 2802 Size: 7918 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2249 2283 6109 6143 1903 Size: 34 312 34 3826 34 3678 Fragments arranged by size: 3826 3678 312 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2063 5810 7020 2063 Size: 3747 1210 2961 Fragments arranged by size: 3747 2961 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7918 BfrBI ATG'CAT Cuts at: 2632 4111 2632 Size: 1479 6439 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6529 136 Size: 107 122 71 631 605 4857 1525 Fragments arranged by size: 4857 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2451 7913 2451 Size: 5462 2456 BlpI GC'TnA_GC Cuts at: 5087 5335 5087 Size: 248 7670 Bme1580I G_kGCm'C Cuts at: 1790 4944 5979 7135 1790 Size: 3154 1035 1156 2573 Fragments arranged by size: 3154 2573 1156 1035 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7918 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2982 3438 3654 Size: 416 53 83 399 24 1565 456 216 Cuts at: 3654 4084 4353 5649 6607 442 Size: 430 269 1296 958 1753 Fragments arranged by size: 1753 1565 1296 958 456 430 416 399 269 216 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7918 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3066 3245 3521 3066 Size: 179 276 7463 Fragments arranged by size: 7463 276 179 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2563 3036 3261 3372 3505 4016 4704 4986 Size: 1324 473 225 111 133 511 688 282 Cuts at: 4986 6578 1239 Size: 1592 2579 Fragments arranged by size: 2579 1592 1324 688 511 473 282 225 133 111 Bpu10I CC'TnA_GC Cuts at: 2591 2802 3977 2591 Size: 211 1175 6532 Fragments arranged by size: 6532 1175 211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2084 4195 4514 4640 6043 6908 7206 2084 Size: 2111 319 126 1403 865 298 2796 Fragments arranged by size: 2796 2111 1403 865 319 298 126 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2055 3932 6581 882 Size: 617 556 1877 2649 2219 Fragments arranged by size: 2649 2219 1877 617 556 BsaAI yAC'GTr Cuts at: 493 1204 5709 493 Size: 711 4505 2702 Fragments arranged by size: 4505 2702 711 BsaBI GATnn'nnATC Cuts at: 7912 7912 Size: 7918 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5725 6107 6742 275 Size: 53 83 186 936 4192 382 635 1451 Fragments arranged by size: 4192 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2298 6351 6749 7023 7170 7581 2298 Size: 4053 398 274 147 411 2635 Fragments arranged by size: 4053 2635 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2845 2875 1178 Size: 30 165 30 1442 30 6221 Fragments arranged by size: 6221 1442 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2034 2853 1186 Size: 848 819 6251 Fragments arranged by size: 6251 848 819 BseYI C'CCAG_C Cuts at: 4450 4579 7121 7617 4450 Size: 129 2542 496 4751 Fragments arranged by size: 4751 2542 496 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5368 6129 7157 1540 Size: 275 186 3367 761 1028 2301 Fragments arranged by size: 3367 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3273 4944 5979 6064 7135 3273 Size: 1671 1035 85 1071 4056 Fragments arranged by size: 4056 1671 1071 1035 85 BsmI GAATG_Cn' Cuts at: 4103 4676 5429 5522 4103 Size: 573 753 93 6499 Fragments arranged by size: 6499 753 573 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2296 2296 Size: 7918 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3273 4944 5237 5979 6064 7135 1391 Size: 399 1483 1671 293 742 85 1071 2174 Fragments arranged by size: 2174 1671 1483 1071 742 399 293 85 BspEI T'CCGG_A Cuts at: 7581 7581 Size: 7918 BspHI T'CATG_A Cuts at: 2425 2955 3174 3193 4767 5807 7537 2425 Size: 530 219 19 1574 1040 1730 2806 Fragments arranged by size: 2806 1730 1574 1040 530 219 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3984 4923 5273 844 Size: 3140 939 350 3489 Fragments arranged by size: 3489 3140 939 350 BsrBI CCG'CTC Cuts at: 1394 1823 5805 1394 Size: 429 3982 3507 Fragments arranged by size: 3982 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2570 6413 6587 65 Size: 1183 1322 3843 174 1396 Fragments arranged by size: 3843 1396 1322 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2256 6562 6749 1700 Size: 110 446 4306 187 2869 Fragments arranged by size: 4306 2869 446 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7918 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7918 BssSI C'ACGA_G Cuts at: 5978 6990 5978 Size: 1012 6906 BstAPI GCAn_nnn'nTGC Cuts at: 794 3827 794 Size: 3033 4885 BstBI TT'CG_AA Cuts at: 6722 6722 Size: 7918 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7918 BstXI CCAn_nnnn'nTGG Cuts at: 2995 3152 3285 4579 4702 2995 Size: 157 133 1294 123 6211 Fragments arranged by size: 6211 1294 157 133 123 BstYI r'GATC_y Cuts at: 1972 2451 3158 3289 4127 5062 5248 5615 6000 Size: 479 707 131 838 935 186 367 385 Cuts at: 6000 6017 7469 7555 7567 7913 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 935 838 707 479 385 367 346 186 131 86 17 12 BstZ17I GTA'TAC Cuts at: 5191 5191 Size: 7918 BtgI C'CryG_G Cuts at: 513 1065 3162 4266 513 Size: 552 2097 1104 4165 Fragments arranged by size: 4165 2097 1104 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7179 Fragments arranged by size: 7179 683 56 BtsI GCAGTG_nn' Cuts at: 5434 6228 6248 5434 Size: 794 20 7104 Fragments arranged by size: 7104 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7918 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7883 DraI TTT'AAA Cuts at: 5222 5222 Size: 7918 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6925 809 Size: 507 5609 1802 Fragments arranged by size: 5609 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2254 2287 3165 Size: 54 1425 50 275 18 424 33 878 Cuts at: 3165 4138 4569 5365 6254 7883 8 Size: 973 431 796 889 1629 43 Fragments arranged by size: 1629 1425 973 889 878 796 431 424 275 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 5365 1537 Size: 275 3553 4090 Fragments arranged by size: 4090 3553 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 2406 2759 4461 4952 5157 5848 1353 Size: 846 207 353 1702 491 205 691 3423 Fragments arranged by size: 3423 1702 846 691 491 353 207 205 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6505 6879 7025 387 Size: 1064 138 4916 374 146 1280 Fragments arranged by size: 4916 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2525 2563 3036 3261 3372 3404 3505 Size: 393 893 38 473 225 111 32 101 Cuts at: 3505 3885 4016 4704 4986 5981 6578 7365 7633 Size: 380 131 688 282 995 597 787 268 Cuts at: 7633 1239 Size: 1524 Fragments arranged by size: 1524 995 893 787 688 597 473 393 380 282 268 225 131 111 101 38 32 EcoNI CCTnn'n_nnAGG Cuts at: 3040 3257 3040 Size: 217 7701 EcoO109I rG'GnC_Cy Cuts at: 3472 5199 3472 Size: 1727 6191 EcoRI G'AATT_C Cuts at: 4848 5227 5605 4848 Size: 379 378 7161 Fragments arranged by size: 7161 379 378 FspI TGC'GCA Cuts at: 4934 6424 4934 Size: 1490 6428 HaeII r_GCGC'y Cuts at: 1678 7065 1678 Size: 5387 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2354 2386 2656 2688 3663 Size: 32 135 32 782 32 270 32 975 Cuts at: 3663 3695 4387 4419 6559 6591 6633 6665 1373 Size: 32 692 32 2140 32 42 32 2626 Fragments arranged by size: 2626 2140 975 782 692 270 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4965 5262 5508 669 Size: 988 281 3027 297 246 3079 Fragments arranged by size: 3079 3027 988 297 281 246 HindIII A'AGCT_T Cuts at: 748 4346 748 Size: 3598 4320 HpaI GTT'AAC Cuts at: 5508 5508 Size: 7918 KpnI G_GTAC'C Cuts at: 5243 5243 Size: 7918 MfeI C'AATT_G Cuts at: 3615 5517 3615 Size: 1902 6016 MluI A'CGCG_T Cuts at: 7794 7794 Size: 7918 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2900 2980 5112 7032 7216 1305 Size: 1595 80 2132 1920 184 2007 Fragments arranged by size: 2132 2007 1920 1595 184 80 MscI TGG'CCA Cuts at: 10 64 1489 1832 2289 3167 4571 10 Size: 54 1425 343 457 878 1404 3357 Fragments arranged by size: 3357 1425 1404 878 457 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2754 3173 3953 4307 4643 5876 6235 Size: 648 1588 419 780 354 336 1233 359 Cuts at: 6235 6394 518 Size: 159 2042 Fragments arranged by size: 2042 1588 1233 780 648 419 359 354 336 159 MspA1I CmG'CkG Cuts at: 1606 3056 3967 4930 5006 5334 6011 7159 7404 Size: 1450 911 963 76 328 677 1148 245 Cuts at: 7404 1606 Size: 2120 Fragments arranged by size: 2120 1450 1148 963 911 677 328 245 76 NaeI GCC'GGC Cuts at: 2258 2258 Size: 7918 NcoI C'CATG_G Cuts at: 513 1065 3162 4266 513 Size: 552 2097 1104 4165 Fragments arranged by size: 4165 2097 1104 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7918 NgoMIV G'CCGG_C Cuts at: 2256 2256 Size: 7918 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7918 NotI GC'GGCC_GC Cuts at: 5365 5365 Size: 7918 NsiI A_TGCA'T Cuts at: 2634 4113 2634 Size: 1479 6439 NspI r_CATG'y Cuts at: 1132 1229 2420 2636 3556 3958 4646 4925 5276 Size: 97 1191 216 920 402 688 279 351 Cuts at: 5276 6821 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1191 920 688 402 351 279 216 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7918 PciI A'CATG_T Cuts at: 4642 6817 4642 Size: 2175 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 4422 1108 Size: 3314 4604 PfoI T'CCnGG_A Cuts at: 2622 2667 3277 4681 2622 Size: 45 610 1404 5859 Fragments arranged by size: 5859 1404 610 45 PmeI GTTT'AAAC Cuts at: 5222 5222 Size: 7918 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4512 4544 5987 6019 7500 7532 4512 Size: 32 1443 32 1481 32 4898 Fragments arranged by size: 4898 1481 1443 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7918 PsiI TTA'TAA Cuts at: 5488 5488 Size: 7918 PstI C_TGCA'G Cuts at: 830 3995 5270 830 Size: 3165 1275 3478 Fragments arranged by size: 3478 3165 1275 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7918 PvuII CAG'CTG Cuts at: 3056 3967 5006 3056 Size: 911 1039 5968 Fragments arranged by size: 5968 1039 911 SalI G'TCGA_C Cuts at: 1936 5260 1936 Size: 3324 4594 SapI GCTCTTCn'nnn_ Cuts at: 2199 2406 4461 2199 Size: 207 2055 5656 Fragments arranged by size: 5656 2055 207 SbfI CC_TGCA'GG Cuts at: 5270 5270 Size: 7918 ScaI AGT'ACT Cuts at: 3697 4037 4803 6166 7878 3697 Size: 340 766 1363 1712 3737 Fragments arranged by size: 3737 1712 1363 766 340 SexAI A'CCwGG_T Cuts at: 3258 3258 Size: 7918 SfcI C'TryA_G Cuts at: 826 1045 3991 4841 5266 6401 7082 7273 826 Size: 219 2946 850 425 1135 681 191 1471 Fragments arranged by size: 2946 1471 1135 850 681 425 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7918 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7918 SmaI CCC'GGG Cuts at: 1872 5245 1872 Size: 3373 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2099 4210 4529 4655 6022 6923 Size: 197 926 156 2111 319 126 1367 901 Cuts at: 6923 7185 820 Size: 262 1553 Fragments arranged by size: 2111 1553 1367 926 901 319 262 197 156 126 SnaBI TAC'GTA Cuts at: 493 5709 493 Size: 5216 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7918 SphI G_CATG'C Cuts at: 1132 2636 5276 1132 Size: 1504 2640 3774 Fragments arranged by size: 3774 2640 1504 SspI AAT'ATT Cuts at: 5 52 5842 5 Size: 47 5790 2081 Fragments arranged by size: 5790 2081 47 StuI AGG'CCT Cuts at: 1517 2343 2984 3072 4731 1517 Size: 826 641 88 1659 4704 Fragments arranged by size: 4704 1659 826 641 88 StyI C'CwwG_G Cuts at: 513 1065 3162 4266 5037 5154 5357 513 Size: 552 2097 1104 771 117 203 3074 Fragments arranged by size: 3074 2097 1104 771 552 203 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3328 4571 5945 5962 6115 6300 6745 Size: 1255 1748 1243 1374 17 153 185 445 Cuts at: 6745 325 Size: 1498 Fragments arranged by size: 1748 1498 1374 1255 1243 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2174 3540 3695 Size: 274 80 33 51 1443 197 1366 155 Cuts at: 3695 3998 4035 4463 4801 6164 7876 96 Size: 303 37 428 338 1363 1712 138 Fragments arranged by size: 1712 1443 1366 1363 428 338 303 274 197 155 138 80 51 37 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7878 7910 7878 Size: 32 7886 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7918 XbaI T'CTAG_A Cuts at: 5254 5254 Size: 7918 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4109 4109 Size: 7918 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7918 XmaI C'CCGG_G Cuts at: 1870 5243 1870 Size: 3373 4545 XmnI GAAnn'nnTTC Cuts at: 2730 6047 2730 Size: 3317 4601 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5726 276 Size: 53 83 186 936 4192 2468 Fragments arranged by size: 4192 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI