(Circular) (Six-base) MAPSORT of: pFN21AA0727.seq Check: 6591 from: 1 to: 7723 pFN21AA0727 7723 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5533 278 Size: 53 83 186 936 3997 2468 Fragments arranged by size: 3997 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3732 5066 1937 Size: 1795 1334 4594 Fragments arranged by size: 4594 1795 1334 Acc65I G'GTAC_C Cuts at: 5044 5044 Size: 7723 AclI AA'CG_TT Cuts at: 1563 5022 5850 6223 1563 Size: 3459 828 373 3063 Fragments arranged by size: 3459 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3272 3669 3948 4783 5786 7170 7438 1632 Size: 1640 397 279 835 1003 1384 268 1917 Fragments arranged by size: 1917 1640 1384 1003 835 397 279 268 AflII C'TTAA_G Cuts at: 820 1017 2420 820 Size: 197 1403 6123 Fragments arranged by size: 6123 1403 197 AflIII A'CryG_T Cuts at: 1203 6622 7599 1203 Size: 5419 977 1327 Fragments arranged by size: 5419 1327 977 AgeI A'CCGG_T Cuts at: 6554 6554 Size: 7723 AhdI GACnn_n'nnGTC Cuts at: 6452 6452 Size: 7723 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7723 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2376 1184 Size: 1192 6531 AlwNI CAG_nnn'CTG Cuts at: 5466 7038 5466 Size: 1572 6151 ApaLI G'TGCA_C Cuts at: 4933 5780 6936 4933 Size: 847 1156 5720 Fragments arranged by size: 5720 1156 847 ApoI r'AATT_y Cuts at: 1493 2027 2741 2839 2846 3489 3516 3712 3935 Size: 534 714 98 7 643 27 196 223 Cuts at: 3935 4038 5032 5258 5410 7519 1493 Size: 103 994 226 152 2109 1697 Fragments arranged by size: 2109 1697 994 714 643 534 226 223 196 152 103 98 27 7 AseI AT'TA_AT Cuts at: 160 1032 6277 160 Size: 872 5245 1606 Fragments arranged by size: 5245 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7723 AvaI C'yCGr_G Cuts at: 1870 1943 2072 2571 4454 4993 5015 5048 1870 Size: 73 129 499 1883 539 22 33 4545 Fragments arranged by size: 4545 1883 539 499 129 73 33 22 BamHI G'GATC_C Cuts at: 3285 4666 5053 5420 3285 Size: 1381 387 367 5588 Fragments arranged by size: 5588 1381 387 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3825 4136 5044 6499 618 Size: 325 217 627 2038 311 908 1455 1842 Fragments arranged by size: 2038 1842 1455 908 627 325 311 217 BanII G_rGCy'C Cuts at: 1391 2710 4461 5042 1391 Size: 1319 1751 581 4072 Fragments arranged by size: 4072 1751 1319 581 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2712 4037 4629 4700 928 Size: 971 813 1325 592 71 3951 Fragments arranged by size: 3951 1325 971 813 592 71 BbvCI CC'TCA_GC Cuts at: 5006 5006 Size: 7723 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3373 3407 5914 5948 1903 Size: 34 1436 34 2507 34 3678 Fragments arranged by size: 3678 2507 1436 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5615 6825 5615 Size: 1210 6513 BclI T'GATC_A Cuts at: 1548 1548 Size: 7723 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6334 136 Size: 107 122 71 631 605 4662 1525 Fragments arranged by size: 4662 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7718 7718 Size: 7723 BlpI GC'TnA_GC Cuts at: 5140 5140 Size: 7723 Bme1580I G_kGCm'C Cuts at: 1790 4090 4139 4937 5016 5784 6940 1790 Size: 2300 49 798 79 768 1156 2573 Fragments arranged by size: 2573 2300 1156 798 768 79 49 BmgBI CAC'GTC Cuts at: 1318 4591 1318 Size: 3273 4450 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2373 4898 5454 Size: 416 53 83 399 24 956 2525 556 Cuts at: 5454 6412 442 Size: 958 1753 Fragments arranged by size: 2525 1753 958 956 556 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7723 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2247 2247 Size: 7723 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2651 3515 4445 6383 1239 Size: 1412 864 930 1938 2579 Fragments arranged by size: 2579 1938 1412 930 864 Bpu10I CC'TnA_GC Cuts at: 5006 5006 Size: 7723 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2464 3617 5848 6713 7011 2464 Size: 1153 2231 865 298 3176 Fragments arranged by size: 3176 2231 1153 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2218 6386 882 Size: 617 719 4168 2219 Fragments arranged by size: 4168 2219 719 617 BsaAI yAC'GTr Cuts at: 493 1204 4282 5514 493 Size: 711 3078 1232 2702 Fragments arranged by size: 3078 2702 1232 711 BsaBI GATnn'nnATC Cuts at: 7717 7717 Size: 7723 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3027 4251 5530 5912 Size: 53 83 186 936 1494 1224 1279 382 Cuts at: 5912 6547 275 Size: 635 1451 Fragments arranged by size: 1494 1451 1279 1224 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2955 6156 6554 6828 6975 7386 2955 Size: 3201 398 274 147 411 3292 Fragments arranged by size: 3292 3201 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2048 2078 2124 2154 4824 Size: 30 165 30 645 30 46 30 2670 Cuts at: 4824 4836 4854 4866 1178 Size: 12 18 12 4035 Fragments arranged by size: 4035 2670 645 165 46 30 30 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2804 4337 1186 Size: 1618 1533 4572 Fragments arranged by size: 4572 1618 1533 BseYI C'CCAG_C Cuts at: 4362 4968 6926 7422 4362 Size: 606 1958 496 4663 Fragments arranged by size: 4663 1958 606 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2813 4904 4933 1163 Size: 573 1077 2091 29 3953 Fragments arranged by size: 3953 2091 1077 573 29 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5173 5934 6962 1540 Size: 275 186 3172 761 1028 2301 Fragments arranged by size: 3172 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2710 3633 4937 5784 5869 6940 2710 Size: 923 1304 847 85 1071 3493 Fragments arranged by size: 3493 1304 1071 923 847 85 BsmI GAATG_Cn' Cuts at: 4377 5234 5327 4377 Size: 857 93 6773 Fragments arranged by size: 6773 857 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2068 3018 4943 4945 2068 Size: 950 1925 2 4846 Fragments arranged by size: 4846 1925 950 2 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2710 3633 4090 4139 4461 4937 5016 Size: 399 920 923 457 49 322 476 79 Cuts at: 5016 5042 5784 5869 6940 1391 Size: 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 923 920 742 476 457 399 322 85 79 49 26 BspEI T'CCGG_A Cuts at: 7386 7386 Size: 7723 BspHI T'CATG_A Cuts at: 5612 7342 5612 Size: 1730 5993 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2475 4401 5078 844 Size: 1631 1926 677 3489 Fragments arranged by size: 3489 1926 1631 677 BsrBI CCG'CTC Cuts at: 1394 1823 5610 1394 Size: 429 3787 3507 Fragments arranged by size: 3787 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3220 3360 6218 6392 65 Size: 1183 1972 140 2858 174 1396 Fragments arranged by size: 2858 1972 1396 1183 174 140 BsrFI r'CCGG_y Cuts at: 1700 1810 3822 4197 6367 6554 1700 Size: 110 2012 375 2170 187 2869 Fragments arranged by size: 2869 2170 2012 375 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7723 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7723 BssSI C'ACGA_G Cuts at: 3335 4226 5783 6795 3335 Size: 891 1557 1012 4263 Fragments arranged by size: 4263 1557 1012 891 BstAPI GCAn_nnn'nTGC Cuts at: 794 3881 4415 794 Size: 3087 534 4102 Fragments arranged by size: 4102 3087 534 BstBI TT'CG_AA Cuts at: 3514 4048 6527 3514 Size: 534 2479 4710 Fragments arranged by size: 4710 2479 534 BstEII G'GTnAC_C Cuts at: 1184 2525 3673 1184 Size: 1341 1148 5234 Fragments arranged by size: 5234 1341 1148 BstYI r'GATC_y Cuts at: 1972 2793 3285 4666 5053 5420 5805 5822 7274 Size: 821 492 1381 387 367 385 17 1452 Cuts at: 7274 7360 7372 7718 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1381 821 492 387 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2090 2787 4516 2090 Size: 697 1729 5297 Fragments arranged by size: 5297 1729 697 BtgI C'CryG_G Cuts at: 513 1065 2004 3121 4246 4422 4944 513 Size: 552 939 1117 1125 176 522 3292 Fragments arranged by size: 3292 1125 1117 939 552 522 176 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3549 487 Size: 683 56 2323 4661 Fragments arranged by size: 4661 2323 683 56 BtsI GCAGTG_nn' Cuts at: 5239 6033 6053 5239 Size: 794 20 6909 Fragments arranged by size: 6909 794 20 ClaI AT'CG_AT Cuts at: 1554 4606 1554 Size: 3052 4671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4109 4144 529 Size: 35 3545 35 4108 Fragments arranged by size: 4108 3545 35 35 DraI TTT'AAA Cuts at: 2844 5027 2844 Size: 2183 5540 DraIII CAC_nnn'GTG Cuts at: 4231 4231 Size: 7723 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4787 6730 809 Size: 507 3471 1943 1802 Fragments arranged by size: 3471 1943 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 2364 2964 Size: 54 1425 50 275 18 252 282 600 Cuts at: 2964 4233 4289 5170 6059 7688 8 Size: 1269 56 881 889 1629 43 Fragments arranged by size: 1629 1425 1269 889 881 600 282 275 252 56 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5170 1537 Size: 275 3358 4090 Fragments arranged by size: 4090 3358 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2176 2267 4802 5653 1353 Size: 823 91 2535 851 3423 Fragments arranged by size: 3423 2535 851 823 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3878 6310 6684 6830 387 Size: 1064 138 435 1854 2432 374 146 1280 Fragments arranged by size: 2432 1854 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2708 2708 Size: 7723 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2651 3272 3515 3669 3948 4445 4783 Size: 393 1019 621 243 154 279 497 338 Cuts at: 4783 5786 6383 7170 7438 1239 Size: 1003 597 787 268 1524 Fragments arranged by size: 1524 1019 1003 787 621 597 497 393 338 279 268 243 154 EcoNI CCTnn'n_nnAGG Cuts at: 4397 4397 Size: 7723 EcoO109I rG'GnC_Cy Cuts at: 4327 4467 4327 Size: 140 7583 EcoRI G'AATT_C Cuts at: 2027 2741 3516 3712 3935 5032 5410 2027 Size: 714 775 196 223 1097 378 4340 Fragments arranged by size: 4340 1097 775 714 378 223 196 EcoRV GAT'ATC Cuts at: 2304 2506 3154 2304 Size: 202 648 6873 Fragments arranged by size: 6873 648 202 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2059 2966 2059 Size: 907 6816 FspI TGC'GCA Cuts at: 6229 6229 Size: 7723 HaeII r_GCGC'y Cuts at: 1678 3055 3604 4204 4879 6870 1678 Size: 1377 549 600 675 1991 2531 Fragments arranged by size: 2531 1991 1377 675 600 549 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2124 2156 3058 Size: 32 135 32 476 32 44 32 902 Cuts at: 3058 3090 3776 3808 3853 3885 4687 4719 6364 Size: 32 686 32 45 32 802 32 1645 Cuts at: 6364 6396 6438 6470 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1645 902 802 686 476 135 45 44 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3331 5067 5313 669 Size: 988 281 1393 1736 246 3079 Fragments arranged by size: 3079 1736 1393 988 281 246 HindIII A'AGCT_T Cuts at: 748 2442 2602 748 Size: 1694 160 5869 Fragments arranged by size: 5869 1694 160 HpaI GTT'AAC Cuts at: 5313 5313 Size: 7723 KpnI G_GTAC'C Cuts at: 5048 5048 Size: 7723 MfeI C'AATT_G Cuts at: 2190 5322 2190 Size: 3132 4591 MluI A'CGCG_T Cuts at: 7599 7599 Size: 7723 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2451 3044 4822 6837 7021 1305 Size: 1146 593 1778 2015 184 2007 Fragments arranged by size: 2015 2007 1778 1146 593 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2084 2966 4235 4291 10 Size: 54 1425 343 252 882 1269 56 3442 Fragments arranged by size: 3442 1425 1269 882 343 252 56 54 MslI CAynn'nnrTG Cuts at: 518 1166 5681 6040 6199 518 Size: 648 4515 359 159 2042 Fragments arranged by size: 4515 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3244 3277 5139 5816 6964 7209 1606 Size: 1638 33 1862 677 1148 245 2120 Fragments arranged by size: 2120 1862 1638 1148 677 245 33 NaeI GCC'GGC Cuts at: 3824 3824 Size: 7723 NcoI C'CATG_G Cuts at: 513 1065 2004 3121 4246 513 Size: 552 939 1117 1125 3990 Fragments arranged by size: 3990 1125 1117 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7723 NgoMIV G'CCGG_C Cuts at: 3822 3822 Size: 7723 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7723 NotI GC'GGCC_GC Cuts at: 5170 5170 Size: 7723 NspI r_CATG'y Cuts at: 1132 1229 4153 5081 6626 1132 Size: 97 2924 928 1545 2229 Fragments arranged by size: 2924 2229 1545 928 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7723 PciI A'CATG_T Cuts at: 6622 6622 Size: 7723 PflMI CCAn_nnn'nTGG Cuts at: 1108 4231 1108 Size: 3123 4600 PmeI GTTT'AAAC Cuts at: 5027 5027 Size: 7723 PmlI CAC'GTG Cuts at: 4282 4282 Size: 7723 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5792 5824 7305 7337 5792 Size: 32 1481 32 6178 Fragments arranged by size: 6178 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7723 PsiI TTA'TAA Cuts at: 5293 5293 Size: 7723 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2634 2666 4051 4083 2634 Size: 32 1385 32 6274 Fragments arranged by size: 6274 1385 32 32 PstI C_TGCA'G Cuts at: 830 3228 4335 5075 830 Size: 2398 1107 740 3478 Fragments arranged by size: 3478 2398 1107 740 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7723 PvuII CAG'CTG Cuts at: 3244 3244 Size: 7723 SacI G_AGCT'C Cuts at: 2710 2710 Size: 7723 SalI G'TCGA_C Cuts at: 1936 5065 1936 Size: 3129 4594 SbfI CC_TGCA'GG Cuts at: 5075 5075 Size: 7723 ScaI AGT'ACT Cuts at: 5971 7683 5971 Size: 1712 6011 SfcI C'TryA_G Cuts at: 826 1045 3224 4331 5071 6206 6887 7078 826 Size: 219 2179 1107 740 1135 681 191 1471 Fragments arranged by size: 2179 1471 1135 1107 740 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7723 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7723 SmaI CCC'GGG Cuts at: 1872 5017 5050 1872 Size: 3145 33 4545 Fragments arranged by size: 4545 3145 33 SmlI C'TyrA_G Cuts at: 820 1017 1943 2420 2479 2571 3632 5827 6728 Size: 197 926 477 59 92 1061 2195 901 Cuts at: 6728 6990 820 Size: 262 1553 Fragments arranged by size: 2195 1553 1061 926 901 477 262 197 92 59 SnaBI TAC'GTA Cuts at: 493 5514 493 Size: 5021 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7723 SphI G_CATG'C Cuts at: 1132 4153 5081 1132 Size: 3021 928 3774 Fragments arranged by size: 3774 3021 928 SspI AAT'ATT Cuts at: 5 52 5647 5 Size: 47 5595 2081 Fragments arranged by size: 5595 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7723 StyI C'CwwG_G Cuts at: 513 1065 2004 2629 3036 3121 4071 4167 4246 Size: 552 939 625 407 85 950 96 79 Cuts at: 4246 5162 513 Size: 916 3074 Fragments arranged by size: 3074 950 939 916 625 552 407 96 85 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5750 5767 5920 6105 6550 325 Size: 1255 4170 17 153 185 445 1498 Fragments arranged by size: 4170 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 4680 4906 Size: 274 80 33 51 1443 366 2337 226 Cuts at: 4906 5969 7681 96 Size: 1063 1712 138 Fragments arranged by size: 2337 1712 1443 1063 366 274 226 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4836 4868 7683 7715 4836 Size: 32 2815 32 4844 Fragments arranged by size: 4844 2815 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2060 1316 Size: 744 6979 XbaI T'CTAG_A Cuts at: 5059 5059 Size: 7723 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2434 2434 Size: 7723 XhoI C'TCGA_G Cuts at: 1943 2571 1943 Size: 628 7095 XmaI C'CCGG_G Cuts at: 1870 5015 5048 1870 Size: 3145 33 4545 Fragments arranged by size: 4545 3145 33 XmnI GAAnn'nnTTC Cuts at: 3414 4859 5852 3414 Size: 1445 993 5285 Fragments arranged by size: 5285 1445 993 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5531 276 Size: 53 83 186 936 3997 2468 Fragments arranged by size: 3997 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI