Restriction map for
pFN21AA0714
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII BamHI
BanI BbeI BbrPI BbvCI BcgI
BglI BsaHI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstPI Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SwaI Van91I XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 358 CGCG CG^CG
AclI 5120 AACGTT AA^CGTT
AflII 2146 CTTAAG C^TTAAG
AocI 36 CCTGAGG CC^T[ACGT]AGG
AspI 1774 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 132 CCTAGG C^CTAGG
BalI 316 TGGCCA TGG^CCA
BcnI 666 CCGGG CC^[GC]GG
BfrI 2146 CTTAAG C^TTAAG
BlnI 132 CCTAGG C^CTAGG
BsaBI 2806 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3263 GGTCTC GGTCTC(1/5)
BsgI 1975 GTGCAG GTGCAG(16/14)
BspLU11I 2340 ACATGT A^CATGT
BspMI 3441 ACCTGC ACCTGC(4/8)
BsrDI 4021 GCAATG GCAATG(2/0)
Bst1107I 3437 GTATAC GTA^TAC
BstAPI 496 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 358 CGCG CG^CG
BstZ17I 3437 GTATAC GTA^TAC
Bsu36I 36 CCTGAGG CC^T[ACGT]AGG
CfoI 852 GCGC GCG^C
DraI 1888 TTTAAA TTT^AAA
DsaI 4730 CCATGG C^C[GA][CT]GG
Eco81I 36 CCTGAGG CC^T[ACGT]AGG
EcoNI 1551 CCTATTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2136 GAATTC G^AATTC
EcoT22I 503 ATGCAT ATGCA^T
HgaI 359 GCGTC GACGC(5/10)
HhaI 852 GCGC GCG^C
HinP1I 852 GCGC G^CGC
MamI 2806 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 929 CAATTG C^AATTG
MluNI 316 TGGCCA TGG^CCA
MscI 316 TGGCCA TGG^CCA
MunI 929 CAATTG C^AATTG
MvnI 358 CGCG CG^CG
NciI 666 CCGGG CC^[GC]GG
NcoI 4730 CCATGG C^CATGG
NsiI 503 ATGCAT ATGCA^T
PflFI 1774 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 5120 AACGTT AA^CGTT
SnaBI 3177 TACGTA TAC^GTA
Tth111I 1774 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2793 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3437 GTATAC GT^[AC][GT]AC
5113 GTAGAC
AflIII 2340 ACATGT A^C[GA][CT]GT
3192 ACGTGT
AseI 1804 ATTAAT AT^TAAT
4446 ATTAAT
AsnI 1804 ATTAAT AT^TAAT
4446 ATTAAT
AvaI 4883 CTCGAG C^[CT]CG[GA]G
4895 CTCGAG
BbsI 1771 GAAGAC GAAGAC(2/6)
4405 GAAGAC
BciVI 3879 GGATAC GTATCC(6/5)
4426 GGATAC
BglII 1487 AGATCT A^GATCT
3468 AGATCT
BpuAI 1771 GAAGAC GAAGAC(2/6)
4405 GAAGAC
BsaAI 3177 TACGTA [CT]AC^GT[GA]
3191 TACGTG
BsaWI 2066 TCCGGT [AT]^CCGG[AT]
3674 TCCGGT
BsmFI 3731 GGGAC GGGAC(10/14)
4597 GTCCC
BsoBI 4883 CTCGAG C^[CT]CG[GA]G
4895 CTCGAG
BspHI 348 TCATGA T^CATGA
2400 TCATGA
BstYI 1487 AGATCT [GA]^GATC[CT]
3468 AGATCT
DraII 4595 AGGTCCC [GA]G^G[ACGT]CC[CT]
4607 AGGACCC
DrdI 352 GACCGTCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
925 GACCCAATTGTC
EaeI 54 TGGCCG [CT]^GGCC[GA]
316 TGGCCA
EcoO109I 4595 AGGTCCC [GA]G^G[ACGT]CC[CT]
4607 AGGACCC
EcoRV 2215 GATATC GAT^ATC
2737 GATATC
FauI 624 CCCGC CCCGC(4/6)
5 GCGGG
HincII 1179 GTCAAC GT[CT]^[GA]AC
2236 GTCAAC
HindII 1179 GTCAAC GT[CT]^[GA]AC
2236 GTCAAC
MflI 1487 AGATCT [GA]^GATC[CT]
3468 AGATCT
NdeI 4207 CATATG CA^TATG
4705 CATATG
PaeR7I 4883 CTCGAG C^TCGAG
4895 CTCGAG
PpuMI 4595 AGGTCCC [GA]G^G[AT]CC[CT]
4607 AGGACCC
PshBI 1804 ATTAAT AT^TAAT
4446 ATTAAT
RcaI 348 TCATGA T^CATGA
2400 TCATGA
SacI 1578 GAGCTC GAGCT^C
4633 GAGCTC
TaqI 4884 TCGA T^CGA
4896 TCGA
TthHB8I 4884 TCGA T^CGA
4896 TCGA
XhoI 4883 CTCGAG C^TCGAG
4895 CTCGAG
XhoII 1487 AGATCT [GA]^GATC[CT]
3468 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanII BbvI BclI BfaI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CelII Cfr13I DdeI DpnII DraIII
EarI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI