(Circular) (Six-base) MAPSORT of: pFN21AA0714.seq Check: 129 from: 1 to: 10003 pFN21AA0714 10003 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7813 278 Size: 53 83 186 936 6277 2468 Fragments arranged by size: 6277 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5443 7119 7346 1937 Size: 3506 1676 227 4594 Fragments arranged by size: 4594 3506 1676 227 Acc65I G'GTAC_C Cuts at: 7324 7324 Size: 10003 AclI AA'CG_TT Cuts at: 1563 7126 8130 8503 1563 Size: 5563 1004 373 3063 Fragments arranged by size: 5563 3063 1004 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2187 2565 3195 3554 3660 3723 4275 4554 Size: 555 378 630 359 106 63 552 279 Cuts at: 4554 4733 4989 5385 6163 8066 9450 9718 1632 Size: 179 256 396 778 1903 1384 268 1917 Fragments arranged by size: 1917 1903 1384 778 630 555 552 396 378 359 279 268 256 179 106 63 AflII C'TTAA_G Cuts at: 820 1017 4151 820 Size: 197 3134 6672 Fragments arranged by size: 6672 3134 197 AflIII A'CryG_T Cuts at: 1203 4345 5197 8902 9879 1203 Size: 3142 852 3705 977 1327 Fragments arranged by size: 3705 3142 1327 977 852 AgeI A'CCGG_T Cuts at: 8834 8834 Size: 10003 AhdI GACnn_n'nnGTC Cuts at: 8732 8732 Size: 10003 AleI CACnn'nnGTG Cuts at: 1166 5107 1166 Size: 3941 6062 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5838 1184 Size: 4654 5349 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7005 7037 7005 Size: 32 9971 AlwNI CAG_nnn'CTG Cuts at: 2102 2297 3524 4000 4442 7746 9318 2102 Size: 195 1227 476 442 3304 1572 2787 Fragments arranged by size: 3304 2787 1572 1227 476 442 195 ApaLI G'TGCA_C Cuts at: 8060 9216 8060 Size: 1156 8847 ApoI r'AATT_y Cuts at: 1493 2261 2328 2340 2663 2753 3154 4141 4642 Size: 768 67 12 323 90 401 987 501 Cuts at: 4642 5426 5930 7223 7312 7538 7690 9799 1493 Size: 784 504 1293 89 226 152 2109 1697 Fragments arranged by size: 2109 1697 1293 987 784 768 504 501 401 323 226 152 90 89 67 12 AseI AT'TA_AT Cuts at: 160 1032 3810 6452 8557 160 Size: 872 2778 2642 2105 1606 Fragments arranged by size: 2778 2642 2105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10003 AvaI C'yCGr_G Cuts at: 1870 1943 6888 6900 7328 1870 Size: 73 4945 12 428 4545 Fragments arranged by size: 4945 4545 428 73 12 AvrII C'CTAG_G Cuts at: 2137 2137 Size: 10003 BamHI G'GATC_C Cuts at: 7333 7700 7333 Size: 367 9636 BanI G'GyrC_C Cuts at: 618 943 1160 1787 7324 8779 618 Size: 325 217 627 5537 1455 1842 Fragments arranged by size: 5537 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2900 3587 6642 7322 1391 Size: 1509 687 3055 680 4072 Fragments arranged by size: 4072 3055 1509 687 680 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3783 6417 928 Size: 971 1884 2634 4514 Fragments arranged by size: 4514 2634 1884 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8194 8228 1903 Size: 34 6257 34 3678 Fragments arranged by size: 6257 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5878 6425 7895 9105 5878 Size: 547 1470 1210 6776 Fragments arranged by size: 6776 1470 1210 547 BclI T'GATC_A Cuts at: 1548 2350 3310 6250 1548 Size: 802 960 2940 5301 Fragments arranged by size: 5301 2940 960 802 BfrBI ATG'CAT Cuts at: 2510 2510 Size: 10003 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8614 136 Size: 107 122 71 631 605 6942 1525 Fragments arranged by size: 6942 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3492 5473 9998 3492 Size: 1981 4525 3497 Fragments arranged by size: 4525 3497 1981 BlpI GC'TnA_GC Cuts at: 2821 4501 7096 7420 2821 Size: 1680 2595 324 5404 Fragments arranged by size: 5404 2595 1680 324 Bme1580I G_kGCm'C Cuts at: 1790 8064 9220 1790 Size: 6274 1156 2573 Fragments arranged by size: 6274 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3165 1318 Size: 1847 8156 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5666 6986 7734 Size: 416 53 83 399 24 4249 1320 748 Cuts at: 7734 8692 442 Size: 958 1753 Fragments arranged by size: 4249 1753 1320 958 748 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10003 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2857 4899 2857 Size: 2042 7961 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2765 6125 6626 8663 1239 Size: 1526 3360 501 2037 2579 Fragments arranged by size: 3360 2579 2037 1526 501 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5093 8128 8993 9291 5093 Size: 3035 865 298 5805 Fragments arranged by size: 5805 3035 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5274 8666 882 Size: 617 3775 3392 2219 Fragments arranged by size: 3775 3392 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5184 5198 7794 493 Size: 711 3980 14 2596 2702 Fragments arranged by size: 3980 2702 2596 711 14 BsaBI GATnn'nnATC Cuts at: 4815 9997 4815 Size: 5182 4821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7810 8192 8827 275 Size: 53 83 186 936 6277 382 635 1451 Fragments arranged by size: 6277 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4071 5679 8436 8834 9108 9255 9666 4071 Size: 1608 2757 398 274 147 411 4408 Fragments arranged by size: 4408 2757 1608 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 7005 7035 1178 Size: 30 165 30 5602 30 4146 Fragments arranged by size: 5602 4146 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2762 3744 6164 6650 1186 Size: 1576 982 2420 486 4539 Fragments arranged by size: 4539 2420 1576 982 486 BseYI C'CCAG_C Cuts at: 2944 3176 3699 4988 9206 9702 2944 Size: 232 523 1289 4218 496 3245 Fragments arranged by size: 4218 3245 1289 523 496 232 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4001 1163 Size: 573 2265 7165 Fragments arranged by size: 7165 2265 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7453 8214 9242 1540 Size: 275 186 5452 761 1028 2301 Fragments arranged by size: 5452 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3587 4069 4570 5437 5753 6642 8064 8149 9220 Size: 482 501 867 316 889 1422 85 1071 Cuts at: 9220 3587 Size: 4370 Fragments arranged by size: 4370 1422 1071 889 867 501 482 316 85 BsmI GAATG_Cn' Cuts at: 2859 3251 6028 6333 7514 7607 2859 Size: 392 2777 305 1181 93 5255 Fragments arranged by size: 5255 2777 1181 392 305 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3171 4365 7286 3171 Size: 1194 2921 5888 Fragments arranged by size: 5888 2921 1194 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2900 3587 4069 4570 5437 5753 6642 Size: 399 1110 687 482 501 867 316 889 Cuts at: 6642 7322 8064 8149 9220 1391 Size: 680 742 85 1071 2174 Fragments arranged by size: 2174 1110 1071 889 867 742 687 680 501 482 399 316 85 BspEI T'CCGG_A Cuts at: 9666 9666 Size: 10003 BspHI T'CATG_A Cuts at: 2353 4405 7892 9622 2353 Size: 2052 3487 1730 2734 Fragments arranged by size: 3487 2734 2052 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5455 7358 844 Size: 4611 1903 3489 Fragments arranged by size: 4611 3489 1903 BsrBI CCG'CTC Cuts at: 1394 1823 7890 1394 Size: 429 6067 3507 Fragments arranged by size: 6067 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6033 8498 8672 65 Size: 1183 4785 2465 174 1396 Fragments arranged by size: 4785 2465 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8647 8834 1700 Size: 110 6837 187 2869 Fragments arranged by size: 6837 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2276 3961 4059 6844 7242 96 Size: 2180 1685 98 2785 398 2857 Fragments arranged by size: 2857 2785 2180 1685 398 98 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10003 BssSI C'ACGA_G Cuts at: 8063 9075 8063 Size: 1012 8991 BstAPI GCAn_nnn'nTGC Cuts at: 794 2507 794 Size: 1713 8290 BstBI TT'CG_AA Cuts at: 8807 8807 Size: 10003 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10003 BstXI CCAn_nnnn'nTGG Cuts at: 2952 5881 7076 2952 Size: 2929 1195 5879 Fragments arranged by size: 5879 2929 1195 BstYI r'GATC_y Cuts at: 1972 3492 5473 7333 7700 8085 8102 9554 9640 Size: 1520 1981 1860 367 385 17 1452 86 Cuts at: 9640 9652 9998 1972 Size: 12 346 1977 Fragments arranged by size: 1981 1977 1860 1520 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 5444 5444 Size: 10003 Bsu36I CC'TnA_GG Cuts at: 2042 2042 Size: 10003 BtgI C'CryG_G Cuts at: 513 1065 2004 6735 513 Size: 552 939 4731 3781 Fragments arranged by size: 4731 3781 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9264 Fragments arranged by size: 9264 683 56 BtsI GCAGTG_nn' Cuts at: 4456 4901 5102 5114 6766 6820 7034 7519 8313 Size: 445 201 12 1652 54 214 485 794 Cuts at: 8313 8333 4456 Size: 20 6126 Fragments arranged by size: 6126 1652 794 485 445 214 201 54 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10003 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9968 DraI TTT'AAA Cuts at: 3895 7307 3895 Size: 3412 6591 DraIII CAC_nnn'GTG Cuts at: 2624 5265 6407 2624 Size: 2641 1142 6220 Fragments arranged by size: 6220 2641 1142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2363 2936 9010 809 Size: 507 1047 573 6074 1802 Fragments arranged by size: 6074 1802 1047 573 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2059 2321 7450 Size: 54 1425 50 275 18 229 262 5129 Cuts at: 7450 8339 9968 8 Size: 889 1629 43 Fragments arranged by size: 5129 1629 1425 889 275 262 229 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7450 1537 Size: 275 5638 4090 Fragments arranged by size: 5638 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3214 3279 3575 4152 4326 4890 6182 6282 Size: 1861 65 296 577 174 564 1292 100 Cuts at: 6282 6330 7933 1353 Size: 48 1603 3423 Fragments arranged by size: 3423 1861 1603 1292 577 564 296 174 100 65 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7048 8590 8964 9110 387 Size: 1064 138 5459 1542 374 146 1280 Fragments arranged by size: 5459 1542 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3585 6640 3585 Size: 3055 6948 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 2565 2765 3195 3554 3660 3723 Size: 393 555 378 200 430 359 106 63 Cuts at: 3723 4275 4554 4733 4989 5385 6125 6163 6626 Size: 552 279 179 256 396 740 38 463 Cuts at: 6626 8066 8663 9450 9718 1239 Size: 1440 597 787 268 1524 Fragments arranged by size: 1524 1440 787 740 597 555 552 463 430 396 393 378 359 279 268 256 200 179 106 63 38 EcoNI CCTnn'n_nnAGG Cuts at: 3560 3560 Size: 10003 EcoO109I rG'GnC_Cy Cuts at: 6601 6613 6601 Size: 12 9991 EcoRI G'AATT_C Cuts at: 4141 7312 7690 4141 Size: 3171 378 6454 Fragments arranged by size: 6454 3171 378 EcoRV GAT'ATC Cuts at: 4222 4744 4222 Size: 522 9481 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2327 4147 2327 Size: 1820 8183 FspI TGC'GCA Cuts at: 8509 8509 Size: 10003 HaeII r_GCGC'y Cuts at: 1678 9150 1678 Size: 7472 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2870 2902 3518 3550 4262 Size: 32 135 32 1298 32 616 32 712 Cuts at: 4262 4294 6423 6455 8644 8676 8718 8750 1373 Size: 32 2129 32 2189 32 42 32 2626 Fragments arranged by size: 2626 2189 2129 1298 712 616 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3186 4243 7347 7593 669 Size: 988 281 1248 1057 3104 246 3079 Fragments arranged by size: 3104 3079 1248 1057 988 281 246 HindIII A'AGCT_T Cuts at: 748 2385 3956 4327 748 Size: 1637 1571 371 6424 Fragments arranged by size: 6424 1637 1571 371 HpaI GTT'AAC Cuts at: 7593 7593 Size: 10003 KpnI G_GTAC'C Cuts at: 7328 7328 Size: 10003 MfeI C'AATT_G Cuts at: 2934 7602 2934 Size: 4668 5335 MluI A'CGCG_T Cuts at: 9879 9879 Size: 10003 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3120 5748 6358 6599 9117 9301 1305 Size: 1815 2628 610 241 2518 184 2007 Fragments arranged by size: 2628 2518 2007 1815 610 241 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2323 10 Size: 54 1425 343 491 7690 Fragments arranged by size: 7690 1425 491 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3006 5107 7074 7961 8320 8479 518 Size: 648 1840 2101 1967 887 359 159 2042 Fragments arranged by size: 2101 2042 1967 1840 887 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2489 5230 5835 6646 7419 8096 9244 9489 Size: 883 2741 605 811 773 677 1148 245 Cuts at: 9489 1606 Size: 2120 Fragments arranged by size: 2741 2120 1148 883 811 773 677 605 245 NcoI C'CATG_G Cuts at: 513 1065 2004 6735 513 Size: 552 939 4731 3781 Fragments arranged by size: 4731 3781 939 552 NdeI CA'TA_TG Cuts at: 387 6213 6711 387 Size: 5826 498 3679 Fragments arranged by size: 5826 3679 498 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10003 NotI GC'GGCC_GC Cuts at: 7450 7450 Size: 10003 NsiI A_TGCA'T Cuts at: 2512 2512 Size: 10003 NspI r_CATG'y Cuts at: 1132 1229 4173 4193 4349 7215 7361 8906 1132 Size: 97 2944 20 156 2866 146 1545 2229 Fragments arranged by size: 2944 2866 2229 1545 156 146 97 20 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10003 PciI A'CATG_T Cuts at: 4345 8902 4345 Size: 4557 5446 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 10003 PfoI T'CCnGG_A Cuts at: 5588 5588 Size: 10003 PmeI GTTT'AAAC Cuts at: 7307 7307 Size: 10003 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3285 3317 3708 3740 7005 7037 8072 8104 9585 Size: 32 391 32 3265 32 1035 32 1481 Cuts at: 9585 9617 3285 Size: 32 3671 Fragments arranged by size: 3671 3265 1481 1035 391 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 6601 6613 6601 Size: 12 9991 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10003 PsiI TTA'TAA Cuts at: 2600 3260 5334 6950 7573 2600 Size: 660 2074 1616 623 5030 Fragments arranged by size: 5030 2074 1616 660 623 PspXI vC'TCGA_Gb Cuts at: 6888 6888 Size: 10003 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2260 2292 6936 6968 2260 Size: 32 4644 32 5295 Fragments arranged by size: 5295 4644 32 32 PstI C_TGCA'G Cuts at: 830 2071 2589 5109 7050 7355 830 Size: 1241 518 2520 1941 305 3478 Fragments arranged by size: 3478 2520 1941 1241 518 305 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10003 PvuII CAG'CTG Cuts at: 2489 5230 5835 6646 2489 Size: 2741 605 811 5846 Fragments arranged by size: 5846 2741 811 605 SacI G_AGCT'C Cuts at: 3587 6642 3587 Size: 3055 6948 SalI G'TCGA_C Cuts at: 1936 7345 1936 Size: 5409 4594 SapI GCTCTTCn'nnn_ Cuts at: 3214 3575 6282 3214 Size: 361 2707 6935 Fragments arranged by size: 6935 2707 361 SbfI CC_TGCA'GG Cuts at: 7355 7355 Size: 10003 ScaI AGT'ACT Cuts at: 8251 9963 8251 Size: 1712 8291 SfcI C'TryA_G Cuts at: 826 1045 2067 2585 5105 7046 7204 7351 8486 Size: 219 1022 518 2520 1941 158 147 1135 Cuts at: 8486 9167 9358 826 Size: 681 191 1471 Fragments arranged by size: 2520 1941 1471 1135 1022 681 518 219 191 158 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10003 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10003 SmaI CCC'GGG Cuts at: 1872 7330 1872 Size: 5458 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4151 5072 6888 6900 8107 9008 Size: 197 926 2208 921 1816 12 1207 901 Cuts at: 9008 9270 820 Size: 262 1553 Fragments arranged by size: 2208 1816 1553 1207 926 921 901 262 197 12 SnaBI TAC'GTA Cuts at: 493 5184 7794 493 Size: 4691 2610 2702 Fragments arranged by size: 4691 2702 2610 SpeI A'CTAG_T Cuts at: 152 152 Size: 10003 SphI G_CATG'C Cuts at: 1132 7361 1132 Size: 6229 3774 SspI AAT'ATT Cuts at: 5 52 5179 5571 6781 7927 5 Size: 47 5127 392 1210 1146 2081 Fragments arranged by size: 5127 2081 1210 1146 392 47 StuI AGG'CCT Cuts at: 1517 2047 3744 6190 1517 Size: 530 1697 2446 5330 Fragments arranged by size: 5330 2446 1697 530 StyI C'CwwG_G Cuts at: 513 1065 2004 2137 4122 4205 4225 5941 6735 Size: 552 939 133 1985 83 20 1716 794 Cuts at: 6735 7442 513 Size: 707 3074 Fragments arranged by size: 3074 1985 1716 939 794 707 552 133 83 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3533 5648 7082 8030 8047 8200 8385 Size: 1255 1953 2115 1434 948 17 153 185 Cuts at: 8385 8830 325 Size: 445 1498 Fragments arranged by size: 2115 1953 1498 1434 1255 948 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2276 2962 3961 Size: 274 80 33 51 1443 299 686 999 Cuts at: 3961 4059 5000 5200 6844 6854 7242 8249 9961 Size: 98 941 200 1644 10 388 1007 1712 Cuts at: 9961 96 Size: 138 Fragments arranged by size: 1712 1644 1443 1007 999 941 686 388 299 274 200 138 98 80 51 33 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6745 6777 9963 9995 6745 Size: 32 3186 32 6753 Fragments arranged by size: 6753 3186 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3782 1316 Size: 2466 7537 XbaI T'CTAG_A Cuts at: 4798 7339 4798 Size: 2541 7462 XhoI C'TCGA_G Cuts at: 1943 6888 6900 1943 Size: 4945 12 5046 Fragments arranged by size: 5046 4945 12 XmaI C'CCGG_G Cuts at: 1870 7328 1870 Size: 5458 4545 XmnI GAAnn'nnTTC Cuts at: 5163 5305 7223 8132 5163 Size: 142 1918 909 7034 Fragments arranged by size: 7034 1918 909 142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7811 276 Size: 53 83 186 936 6277 2468 Fragments arranged by size: 6277 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI