Restriction map for
pFN21AA0714
(Linear) (Six-base) MAPSORT of: KIAA0714.seq Check: 600 from: 1 to: 5298
KIAA0714.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 3438 5114 5298
Size: 3438 1676 184
Fragments arranged by size:
3438 1676 184
AclI AA'CG_TT
Cuts at: 0 5121 5298
Size: 5121 177
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 560 1190 1549 1655 1718 2270 2549
Size: 182 378 630 359 106 63 552 279
Cuts at: 2549 2728 2984 3380 4158 5298
Size: 179 256 396 778 1140
Fragments arranged by size:
1140 778 630 552 396 378 359 279
256 182 179 106 63
AflII C'TTAA_G
Cuts at: 0 2146 5298
Size: 2146 3152
AflIII A'CryG_T
Cuts at: 0 2340 3192 5298
Size: 2340 852 2106
Fragments arranged by size:
2340 2106 852
AleI CACnn'nnGTG
Cuts at: 0 3102 5298
Size: 3102 2196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3833 5298
Size: 3833 1465
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5000 5032 5298
Size: 5000 32 266
Fragments arranged by size:
5000 266 32
AlwNI CAG_nnn'CTG
Cuts at: 0 97 292 1519 1995 2437 5298
Size: 97 195 1227 476 442 2861
Fragments arranged by size:
2861 1227 476 442 195 97
ApoI r'AATT_y
Cuts at: 0 256 323 335 658 748 1149 2136 2637
Size: 256 67 12 323 90 401 987 501
Cuts at: 2637 3421 3925 5218 5298
Size: 784 504 1293 80
Fragments arranged by size:
1293 987 784 504 501 401 323 256
90 80 67 12
AseI AT'TA_AT
Cuts at: 0 1805 4447 5298
Size: 1805 2642 851
Fragments arranged by size:
2642 1805 851
AvaI C'yCGr_G
Cuts at: 0 4883 4895 5298
Size: 4883 12 403
Fragments arranged by size:
4883 403 12
AvrII C'CTAG_G
Cuts at: 0 132 5298
Size: 132 5166
BanII G_rGCy'C
Cuts at: 0 895 1582 4637 5298
Size: 895 687 3055 661
Fragments arranged by size:
3055 895 687 661
BbsI GAAGACnn'nnnn_
Cuts at: 0 1778 4412 5298
Size: 1778 2634 886
Fragments arranged by size:
2634 1778 886
BciVI GTATCCnnnnn_n'
Cuts at: 0 3873 4420 5298
Size: 3873 547 878
Fragments arranged by size:
3873 878 547
BclI T'GATC_A
Cuts at: 0 345 1305 4245 5298
Size: 345 960 2940 1053
Fragments arranged by size:
2940 1053 960 345
BfrBI ATG'CAT
Cuts at: 0 505 5298
Size: 505 4793
BglII A'GATC_T
Cuts at: 0 1487 3468 5298
Size: 1487 1981 1830
Fragments arranged by size:
1981 1830 1487
BlpI GC'TnA_GC
Cuts at: 0 816 2496 5091 5298
Size: 816 1680 2595 207
Fragments arranged by size:
2595 1680 816 207
BmgBI CAC'GTC
Cuts at: 0 1160 5298
Size: 1160 4138
BmrI ACTGGGnnnn_n'
Cuts at: 0 3661 4981 5298
Size: 3661 1320 317
Fragments arranged by size:
3661 1320 317
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 852 2894 5298
Size: 852 2042 2404
Fragments arranged by size:
2404 2042 852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 4120 4621 5298
Size: 760 3360 501 677
Fragments arranged by size:
3360 760 677 501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3088 5298
Size: 3088 2210
BsaI GGTCTCn'nnnn_
Cuts at: 0 3269 5298
Size: 3269 2029
BsaAI yAC'GTr
Cuts at: 0 3179 3193 5298
Size: 3179 14 2105
Fragments arranged by size:
3179 2105 14
BsaBI GATnn'nnATC
Cuts at: 0 2810 5298
Size: 2810 2488
BsaWI w'CCGG_w
Cuts at: 0 2066 3674 5298
Size: 2066 1608 1624
Fragments arranged by size:
2066 1624 1608
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5000 5030 5298
Size: 5000 30 268
Fragments arranged by size:
5000 268 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 757 1739 4159 4645 5298
Size: 757 982 2420 486 653
Fragments arranged by size:
2420 982 757 653 486
BseYI C'CCAG_C
Cuts at: 0 939 1171 1694 2983 5298
Size: 939 232 523 1289 2315
Fragments arranged by size:
2315 1289 939 523 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1996 5298
Size: 1996 3302
BsiHKAI G_wGCw'C
Cuts at: 0 1582 2064 2565 3432 3748 4637 5298
Size: 1582 482 501 867 316 889 661
Fragments arranged by size:
1582 889 867 661 501 482 316
BsmI GAATG_Cn'
Cuts at: 0 854 1246 4023 4328 5298
Size: 854 392 2777 305 970
Fragments arranged by size:
2777 970 854 392 305
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1166 2360 5281 5298
Size: 1166 1194 2921 17
Fragments arranged by size:
2921 1194 1166 17
Bsp1286I G_dGCh'C
Cuts at: 0 895 1582 2064 2565 3432 3748 4637 5298
Size: 895 687 482 501 867 316 889 661
Fragments arranged by size:
895 889 867 687 661 501 482 316
BspHI T'CATG_A
Cuts at: 0 348 2400 5298
Size: 348 2052 2898
Fragments arranged by size:
2898 2052 348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3450 5298
Size: 3450 1848
BsrDI GCAATG_nn'
Cuts at: 0 4028 5298
Size: 4028 1270
BsrGI T'GTAC_A
Cuts at: 0 271 1956 2054 4839 5237 5298
Size: 271 1685 98 2785 398 61
Fragments arranged by size:
2785 1685 398 271 98 61
BstAPI GCAn_nnn'nTGC
Cuts at: 0 502 5298
Size: 502 4796
BstXI CCAn_nnnn'nTGG
Cuts at: 0 947 3876 5071 5298
Size: 947 2929 1195 227
Fragments arranged by size:
2929 1195 947 227
BstYI r'GATC_y
Cuts at: 0 1487 3468 5298
Size: 1487 1981 1830
Fragments arranged by size:
1981 1830 1487
BstZ17I GTA'TAC
Cuts at: 0 3439 5298
Size: 3439 1859
Bsu36I CC'TnA_GG
Cuts at: 0 37 5298
Size: 37 5261
BtgI C'CryG_G
Cuts at: 0 4730 5298
Size: 4730 568
BtsI GCAGTG_nn'
Cuts at: 0 2451 2896 3097 3109 4761 4815 5029 5298
Size: 2451 445 201 12 1652 54 214 269
Fragments arranged by size:
2451 1652 445 269 214 201 54 12
DraI TTT'AAA
Cuts at: 0 1890 5298
Size: 1890 3408
DraIII CAC_nnn'GTG
Cuts at: 0 619 3260 4402 5298
Size: 619 2641 1142 896
Fragments arranged by size:
2641 1142 896 619
DrdI GACnn_nn'nnGTC
Cuts at: 0 358 931 5298
Size: 358 573 4367
Fragments arranged by size:
4367 573 358
EaeI y'GGCC_r
Cuts at: 0 54 316 5298
Size: 54 262 4982
Fragments arranged by size:
4982 262 54
EarI CTCTTCn'nnn_
Cuts at: 0 1209 1274 1570 2147 2321 2885 4177 4277
Size: 1209 65 296 577 174 564 1292 100
Cuts at: 4277 4325 5298
Size: 48 973
Fragments arranged by size:
1292 1209 973 577 564 296 174 100
65 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 5043 5298
Size: 5043 255
EcoICRI GAG'CTC
Cuts at: 0 1580 4635 5298
Size: 1580 3055 663
Fragments arranged by size:
3055 1580 663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 560 760 1190 1549 1655 1718 2270
Size: 182 378 200 430 359 106 63 552
Cuts at: 2270 2549 2728 2984 3380 4120 4158 4621 5298
Size: 279 179 256 396 740 38 463 677
Fragments arranged by size:
740 677 552 463 430 396 378 359
279 256 200 182 179 106 63 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1555 5298
Size: 1555 3743
EcoO109I rG'GnC_Cy
Cuts at: 0 4596 4608 5298
Size: 4596 12 690
Fragments arranged by size:
4596 690 12
EcoRI G'AATT_C
Cuts at: 0 2136 5298
Size: 2136 3162
EcoRV GAT'ATC
Cuts at: 0 2217 2739 5298
Size: 2217 522 2559
Fragments arranged by size:
2559 2217 522
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 322 2142 5298
Size: 322 1820 3156
Fragments arranged by size:
3156 1820 322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 865 897 1513 1545 2257 2289 4418 4450
Size: 865 32 616 32 712 32 2129 32
Cuts at: 4450 5298
Size: 848
Fragments arranged by size:
2129 865 848 712 616 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1181 2238 5298
Size: 1181 1057 3060
Fragments arranged by size:
3060 1181 1057
HindIII A'AGCT_T
Cuts at: 0 380 1951 2322 5298
Size: 380 1571 371 2976
Fragments arranged by size:
2976 1571 380 371
MfeI C'AATT_G
Cuts at: 0 929 5298
Size: 929 4369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 3743 4353 4594 5298
Size: 1115 2628 610 241 704
Fragments arranged by size:
2628 1115 704 610 241
MscI TGG'CCA
Cuts at: 0 318 5298
Size: 318 4980
MslI CAynn'nnrTG
Cuts at: 0 1001 3102 5069 5298
Size: 1001 2101 1967 229
Fragments arranged by size:
2101 1967 1001 229
MspA1I CmG'CkG
Cuts at: 0 484 3225 3830 4641 5298
Size: 484 2741 605 811 657
Fragments arranged by size:
2741 811 657 605 484
NcoI C'CATG_G
Cuts at: 0 4730 5298
Size: 4730 568
NdeI CA'TA_TG
Cuts at: 0 4208 4706 5298
Size: 4208 498 592
Fragments arranged by size:
4208 592 498
NsiI A_TGCA'T
Cuts at: 0 507 5298
Size: 507 4791
NspI r_CATG'y
Cuts at: 0 2168 2188 2344 5210 5298
Size: 2168 20 156 2866 88
Fragments arranged by size:
2866 2168 156 88 20
PciI A'CATG_T
Cuts at: 0 2340 5298
Size: 2340 2958
PfoI T'CCnGG_A
Cuts at: 0 3583 5298
Size: 3583 1715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1280 1312 1703 1735 5000 5032 5298
Size: 1280 32 391 32 3265 32 266
Fragments arranged by size:
3265 1280 391 266 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 4596 4608 5298
Size: 4596 12 690
Fragments arranged by size:
4596 690 12
PsiI TTA'TAA
Cuts at: 0 595 1255 3329 4945 5298
Size: 595 660 2074 1616 353
Fragments arranged by size:
2074 1616 660 595 353
PspXI vC'TCGA_Gb
Cuts at: 0 4883 5298
Size: 4883 415
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 255 287 4931 4963 5298
Size: 255 32 4644 32 335
Fragments arranged by size:
4644 335 255 32 32
PstI C_TGCA'G
Cuts at: 0 66 584 3104 5045 5298
Size: 66 518 2520 1941 253
Fragments arranged by size:
2520 1941 518 253 66
PvuII CAG'CTG
Cuts at: 0 484 3225 3830 4641 5298
Size: 484 2741 605 811 657
Fragments arranged by size:
2741 811 657 605 484
SacI G_AGCT'C
Cuts at: 0 1582 4637 5298
Size: 1582 3055 661
Fragments arranged by size:
3055 1582 661
SapI GCTCTTCn'nnn_
Cuts at: 0 1209 1570 4277 5298
Size: 1209 361 2707 1021
Fragments arranged by size:
2707 1209 1021 361
SfcI C'TryA_G
Cuts at: 0 62 580 3100 5041 5199 5298
Size: 62 518 2520 1941 158 99
Fragments arranged by size:
2520 1941 518 158 99 62
SmlI C'TyrA_G
Cuts at: 0 2146 3067 4883 4895 5298
Size: 2146 921 1816 12 403
Fragments arranged by size:
2146 1816 921 403 12
SnaBI TAC'GTA
Cuts at: 0 3179 5298
Size: 3179 2119
SspI AAT'ATT
Cuts at: 0 3174 3566 4776 5298
Size: 3174 392 1210 522
Fragments arranged by size:
3174 1210 522 392
StuI AGG'CCT
Cuts at: 0 42 1739 4185 5298
Size: 42 1697 2446 1113
Fragments arranged by size:
2446 1697 1113 42
StyI C'CwwG_G
Cuts at: 0 132 2117 2200 2220 3936 4730 5298
Size: 132 1985 83 20 1716 794 568
Fragments arranged by size:
1985 1716 794 568 132 83 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1528 3643 5077 5298
Size: 1528 2115 1434 221
Fragments arranged by size:
2115 1528 1434 221
TatI w'GTAC_w
Cuts at: 0 271 957 1956 2054 2995 3195 4839 4849
Size: 271 686 999 98 941 200 1644 10
Cuts at: 4849 5237 5298
Size: 388 61
Fragments arranged by size:
1644 999 941 686 388 271 200 98
61 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4740 4772 5298
Size: 4740 32 526
Fragments arranged by size:
4740 526 32
Tth111I GACn'n_nGTC
Cuts at: 0 1777 5298
Size: 1777 3521
XbaI T'CTAG_A
Cuts at: 0 2793 5298
Size: 2793 2505
XhoI C'TCGA_G
Cuts at: 0 4883 4895 5298
Size: 4883 12 403
Fragments arranged by size:
4883 403 12
XmnI GAAnn'nnTTC
Cuts at: 0 3158 3300 5218 5298
Size: 3158 142 1918 80
Fragments arranged by size:
3158 1918 142 80
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AleI AlfI AloI
AlwNI ApoI AseI AvaI AvrII BanII BbsI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrGI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI
DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PciI PfoI PpiI PpuMI PsiI PspXI PsrI
PstI PvuII SacI SapI SfcI SmlI SnaBI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AhdI ApaI ApaLI
AscI AsiSI BaeI BamHI BanI BbeI BbvCI BcgI
BglI Bme1580I BmtI Bpu10I BsaHI BsiEI BsiWI BspEI
BsrBI BsrFI BssHII BssSI BstBI BstEII BtgZI ClaI
CspCI EagI FseI FspI FspAI HaeII HpaI KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PflMI PmeI PmlI PshAI PspOMI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI SwaI
XcmI XmaI ZraI