(Circular) (Six-base) MAPSORT of: pFN21AA0674.seq Check: 668 from: 1 to: 8362 pFN21AA0674 8362 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2491 4473 4951 2491 Size: 1982 478 5902 Fragments arranged by size: 5902 1982 478 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6172 278 Size: 53 83 186 936 4636 2468 Fragments arranged by size: 4636 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5705 1937 Size: 3768 4594 Acc65I G'GTAC_C Cuts at: 5683 5683 Size: 8362 AclI AA'CG_TT Cuts at: 1563 6489 6862 1563 Size: 4926 373 3063 Fragments arranged by size: 4926 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3224 3555 3848 4055 4511 4766 5286 6425 Size: 1592 331 293 207 456 255 520 1139 Cuts at: 6425 7809 8077 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1592 1384 1139 520 456 331 293 268 255 207 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8165 AflIII A'CryG_T Cuts at: 1203 7261 8238 1203 Size: 6058 977 1327 Fragments arranged by size: 6058 1327 977 AgeI A'CCGG_T Cuts at: 5399 7193 5399 Size: 1794 6568 AhdI GACnn_n'nnGTC Cuts at: 7091 7091 Size: 8362 AleI CACnn'nnGTG Cuts at: 1166 4070 1166 Size: 2904 5458 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3665 1184 Size: 2481 5881 AlwNI CAG_nnn'CTG Cuts at: 2099 4516 5045 6105 7677 2099 Size: 2417 529 1060 1572 2784 Fragments arranged by size: 2784 2417 1572 1060 529 ApaI G_GGCC'C Cuts at: 5124 5124 Size: 8362 ApaLI G'TGCA_C Cuts at: 6419 7575 6419 Size: 1156 7206 ApoI r'AATT_y Cuts at: 1493 2114 2288 3558 4124 4293 5671 5897 6049 Size: 621 174 1270 566 169 1378 226 152 Cuts at: 6049 8158 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1378 1270 621 566 226 174 169 152 AseI AT'TA_AT Cuts at: 160 1032 6916 160 Size: 872 5884 1606 Fragments arranged by size: 5884 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8362 AvaI C'yCGr_G Cuts at: 1870 1943 4350 5309 5687 1870 Size: 73 2407 959 378 4545 Fragments arranged by size: 4545 2407 959 378 73 AvrII C'CTAG_G Cuts at: 5149 5149 Size: 8362 BamHI G'GATC_C Cuts at: 5692 6059 5692 Size: 367 7995 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2057 3280 3310 4948 5683 Size: 325 217 627 270 1223 30 1638 735 Cuts at: 5683 7138 618 Size: 1455 1842 Fragments arranged by size: 1842 1638 1455 1223 735 627 325 270 217 30 BanII G_rGCy'C Cuts at: 1391 3306 4095 5124 5148 5681 1391 Size: 1915 789 1029 24 533 4072 Fragments arranged by size: 4072 1915 1029 789 533 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4870 5583 928 Size: 971 2971 713 3707 Fragments arranged by size: 3707 2971 971 713 BbvCI CC'TCA_GC Cuts at: 3285 4888 5046 5358 3285 Size: 1603 158 312 6289 Fragments arranged by size: 6289 1603 312 158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2648 2682 2817 2851 3437 3471 6553 Size: 34 711 34 135 34 586 34 3082 Cuts at: 6553 6587 1903 Size: 34 3678 Fragments arranged by size: 3678 3082 711 586 135 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5170 6254 7464 5170 Size: 1084 1210 6068 Fragments arranged by size: 6068 1210 1084 BclI T'GATC_A Cuts at: 1548 1548 Size: 8362 BfrBI ATG'CAT Cuts at: 2249 2249 Size: 8362 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2066 3457 4550 Size: 107 122 71 631 605 394 1391 1093 Cuts at: 4550 6973 136 Size: 2423 1525 Fragments arranged by size: 2423 1525 1391 1093 631 605 394 122 107 71 BglII A'GATC_T Cuts at: 4045 8357 4045 Size: 4312 4050 BlpI GC'TnA_GC Cuts at: 4401 5211 5779 4401 Size: 810 568 6984 Fragments arranged by size: 6984 810 568 Bme1580I G_kGCm'C Cuts at: 1790 4953 5124 6423 7579 1790 Size: 3163 171 1299 1156 2573 Fragments arranged by size: 3163 2573 1299 1156 171 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8362 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3309 4408 6093 Size: 416 53 83 399 24 1892 1099 1685 Cuts at: 6093 7051 442 Size: 958 1753 Fragments arranged by size: 1892 1753 1685 1099 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8362 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5071 5474 5071 Size: 403 7959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2766 3290 4199 4532 4736 7022 1239 Size: 1527 524 909 333 204 2286 2579 Fragments arranged by size: 2579 2286 1527 909 524 333 204 Bpu10I CC'TnA_GC Cuts at: 3285 4888 5046 5142 5358 3285 Size: 1603 158 96 216 6289 Fragments arranged by size: 6289 1603 216 158 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2808 4148 4242 4893 5357 6487 7352 7650 2808 Size: 1340 94 651 464 1130 865 298 3520 Fragments arranged by size: 3520 1340 1130 865 651 464 298 94 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3964 7025 882 Size: 617 2465 3061 2219 Fragments arranged by size: 3061 2465 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6153 493 Size: 711 4949 2702 Fragments arranged by size: 4949 2702 711 BsaBI GATnn'nnATC Cuts at: 8356 8356 Size: 8362 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5599 6169 6551 7186 Size: 53 83 186 936 4066 570 382 635 Cuts at: 7186 275 Size: 1451 Fragments arranged by size: 4066 1451 936 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4146 5399 6795 7193 7467 7614 8025 4146 Size: 1253 1396 398 274 147 411 4483 Fragments arranged by size: 4483 1396 1253 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8137 Fragments arranged by size: 8137 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3947 4196 4292 4295 4980 5195 5327 5579 Size: 2761 249 96 3 685 215 132 252 Cuts at: 5579 5582 1186 Size: 3 3966 Fragments arranged by size: 3966 2761 685 252 249 215 132 96 3 3 BseYI C'CCAG_C Cuts at: 2644 2724 2740 3441 3469 5465 5478 5656 7565 Size: 80 16 701 28 1996 13 178 1909 Cuts at: 7565 8061 2644 Size: 496 2945 Fragments arranged by size: 2945 1996 1909 701 496 178 80 28 16 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4094 1163 Size: 573 2358 5431 Fragments arranged by size: 5431 2358 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5812 6573 7601 1540 Size: 275 186 3811 761 1028 2301 Fragments arranged by size: 3811 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2216 2550 4095 4464 6423 6508 7579 2216 Size: 334 1545 369 1959 85 1071 2999 Fragments arranged by size: 2999 1959 1545 1071 369 334 85 BsmI GAATG_Cn' Cuts at: 2742 5873 5966 2742 Size: 3131 93 5138 Fragments arranged by size: 5138 3131 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2940 2940 Size: 8362 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2550 3306 4095 4464 4953 5124 Size: 399 426 334 756 789 369 489 171 Cuts at: 5124 5148 5681 6423 6508 7579 1391 Size: 24 533 742 85 1071 2174 Fragments arranged by size: 2174 1071 789 756 742 533 489 426 399 369 334 171 85 24 BspEI T'CCGG_A Cuts at: 8025 8025 Size: 8362 BspHI T'CATG_A Cuts at: 3528 3603 4674 4755 6251 7981 3528 Size: 75 1071 81 1496 1730 3909 Fragments arranged by size: 3909 1730 1496 1071 81 75 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2491 3386 3946 4473 4951 5423 5717 844 Size: 1647 895 560 527 478 472 294 3489 Fragments arranged by size: 3489 1647 895 560 527 478 472 294 BsrBI CCG'CTC Cuts at: 1394 1823 2052 3478 4855 6249 1394 Size: 429 229 1426 1377 1394 3507 Fragments arranged by size: 3507 1426 1394 1377 429 229 BsrDI GCAATG_nn' Cuts at: 65 1248 2508 6857 7031 65 Size: 1183 1260 4349 174 1396 Fragments arranged by size: 4349 1396 1260 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2618 3244 5399 7006 7193 1700 Size: 110 808 626 2155 1607 187 2869 Fragments arranged by size: 2869 2155 1607 808 626 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8362 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8362 BssSI C'ACGA_G Cuts at: 6422 7434 6422 Size: 1012 7350 BstAPI GCAn_nnn'nTGC Cuts at: 794 2246 3317 794 Size: 1452 1071 5839 Fragments arranged by size: 5839 1452 1071 BstBI TT'CG_AA Cuts at: 3562 7166 3562 Size: 3604 4758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8362 BstXI CCAn_nnnn'nTGG Cuts at: 2227 4625 5203 2227 Size: 2398 578 5386 Fragments arranged by size: 5386 2398 578 BstYI r'GATC_y Cuts at: 1972 3744 4045 5692 6059 6444 6461 7913 7999 Size: 1772 301 1647 367 385 17 1452 86 Cuts at: 7999 8011 8357 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1772 1647 1452 385 367 346 301 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4992 5332 5562 4992 Size: 340 230 7792 Fragments arranged by size: 7792 340 230 BtgI C'CryG_G Cuts at: 513 1065 2104 4944 5163 513 Size: 552 1039 2840 219 3712 Fragments arranged by size: 3712 2840 1039 552 219 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7623 Fragments arranged by size: 7623 683 56 BtsI GCAGTG_nn' Cuts at: 2550 2664 3948 4831 5878 6672 6692 2550 Size: 114 1284 883 1047 794 20 4220 Fragments arranged by size: 4220 1284 1047 883 794 114 20 ClaI AT'CG_AT Cuts at: 1554 2254 1554 Size: 700 7662 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8327 DraI TTT'AAA Cuts at: 5521 5666 5521 Size: 145 8217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4354 7369 809 Size: 507 3038 3015 1802 Fragments arranged by size: 3038 3015 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2101 3945 4856 Size: 54 1425 50 275 18 271 1844 911 Cuts at: 4856 5809 6698 8327 8 Size: 953 889 1629 43 Fragments arranged by size: 1844 1629 1425 953 911 889 275 271 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5809 1537 Size: 275 3997 4090 Fragments arranged by size: 4090 3997 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2536 3027 3243 3612 3842 4785 4809 4845 Size: 1183 491 216 369 230 943 24 36 Cuts at: 4845 5499 6292 1353 Size: 654 793 3423 Fragments arranged by size: 3423 1183 943 793 654 491 369 230 216 36 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4438 6949 7323 7469 387 Size: 1064 138 2849 2511 374 146 1280 Fragments arranged by size: 2849 2511 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4093 4093 Size: 8362 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2766 3224 3290 3555 3848 4055 4199 Size: 393 1134 458 66 265 293 207 144 Cuts at: 4199 4511 4532 4736 4766 5286 6425 7022 7809 Size: 312 21 204 30 520 1139 597 787 Cuts at: 7809 8077 1239 Size: 268 1524 Fragments arranged by size: 1524 1139 1134 787 597 520 458 393 312 293 268 265 207 204 144 66 30 21 EcoNI CCTnn'n_nnAGG Cuts at: 2148 4181 2148 Size: 2033 6329 EcoO109I rG'GnC_Cy Cuts at: 2557 4996 5120 5121 5153 5320 5336 2557 Size: 2439 124 1 32 167 16 5583 Fragments arranged by size: 5583 2439 167 124 32 16 1 EcoRI G'AATT_C Cuts at: 5671 6049 5671 Size: 378 7984 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3764 4234 3764 Size: 470 7892 FspI TGC'GCA Cuts at: 3954 4489 6868 3954 Size: 535 2379 5448 Fragments arranged by size: 5448 2379 535 FspAI rTGC'GCAy Cuts at: 4489 4489 Size: 8362 HaeII r_GCGC'y Cuts at: 1678 7509 1678 Size: 5831 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2876 2908 2945 Size: 32 135 32 455 32 817 32 37 Cuts at: 2945 2977 3338 3370 4199 4231 4370 4402 4762 Size: 32 361 32 829 32 139 32 360 Cuts at: 4762 4794 5210 5242 7003 7035 7077 7109 1373 Size: 32 416 32 1761 32 42 32 2626 Fragments arranged by size: 2626 1761 829 817 455 416 361 360 139 135 42 37 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2354 5706 5952 669 Size: 988 281 416 3352 246 3079 Fragments arranged by size: 3352 3079 988 416 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8362 HpaI GTT'AAC Cuts at: 5952 5952 Size: 8362 KpnI G_GTAC'C Cuts at: 5687 5687 Size: 8362 MfeI C'AATT_G Cuts at: 5961 5961 Size: 8362 MluI A'CGCG_T Cuts at: 8238 8238 Size: 8362 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2451 2572 7476 7660 1305 Size: 1146 121 4904 184 2007 Fragments arranged by size: 4904 2007 1146 184 121 MscI TGG'CCA Cuts at: 10 64 1489 1832 2103 10 Size: 54 1425 343 271 6269 Fragments arranged by size: 6269 1425 343 271 54 MslI CAynn'nnrTG Cuts at: 518 1166 2109 3692 4070 6320 6679 6838 518 Size: 648 943 1583 378 2250 359 159 2042 Fragments arranged by size: 2250 2042 1583 943 648 378 359 159 MspA1I CmG'CkG Cuts at: 1606 2096 3151 3359 3445 3944 3973 4021 4373 Size: 490 1055 208 86 499 29 48 352 Cuts at: 4373 4384 4786 5778 6455 7603 7848 1606 Size: 11 402 992 677 1148 245 2120 Fragments arranged by size: 2120 1148 1055 992 677 499 490 402 352 245 208 86 48 29 11 NaeI GCC'GGC Cuts at: 3246 3246 Size: 8362 NcoI C'CATG_G Cuts at: 513 1065 2104 4944 5163 513 Size: 552 1039 2840 219 3712 Fragments arranged by size: 3712 2840 1039 552 219 NdeI CA'TA_TG Cuts at: 387 387 Size: 8362 NgoMIV G'CCGG_C Cuts at: 3244 3244 Size: 8362 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8362 NotI GC'GGCC_GC Cuts at: 5809 5809 Size: 8362 NsiI A_TGCA'T Cuts at: 2251 2251 Size: 8362 NspI r_CATG'y Cuts at: 1132 1229 3667 3905 5720 7265 1132 Size: 97 2438 238 1815 1545 2229 Fragments arranged by size: 2438 2229 1815 1545 238 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8362 PciI A'CATG_T Cuts at: 7261 7261 Size: 8362 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8362 PmeI GTTT'AAAC Cuts at: 5666 5666 Size: 8362 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6431 6463 7944 7976 6431 Size: 32 1481 32 6817 Fragments arranged by size: 6817 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2557 5320 2557 Size: 2763 5599 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8362 PsiI TTA'TAA Cuts at: 5932 5932 Size: 8362 PspOMI G'GGCC_C Cuts at: 5120 5120 Size: 8362 PspXI vC'TCGA_Gb Cuts at: 4350 4350 Size: 8362 PstI C_TGCA'G Cuts at: 830 2302 2929 3450 3972 4014 4378 4470 5714 Size: 1472 627 521 522 42 364 92 1244 Cuts at: 5714 830 Size: 3478 Fragments arranged by size: 3478 1472 1244 627 522 521 364 92 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8362 PvuII CAG'CTG Cuts at: 2096 3151 3359 3445 3973 4021 4373 4384 4786 Size: 1055 208 86 528 48 352 11 402 Cuts at: 4786 2096 Size: 5672 Fragments arranged by size: 5672 1055 528 402 352 208 86 48 11 SacI G_AGCT'C Cuts at: 4095 4095 Size: 8362 SalI G'TCGA_C Cuts at: 1936 5704 1936 Size: 3768 4594 SanDI GG'GwC_CC Cuts at: 5320 5320 Size: 8362 SapI GCTCTTCn'nnn_ Cuts at: 3027 4845 3027 Size: 1818 6544 SbfI CC_TGCA'GG Cuts at: 5714 5714 Size: 8362 ScaI AGT'ACT Cuts at: 6610 8322 6610 Size: 1712 6650 SexAI A'CCwGG_T Cuts at: 4680 4680 Size: 8362 SfcI C'TryA_G Cuts at: 826 1045 2298 2419 2583 2925 3446 3968 4010 Size: 219 1253 121 164 342 521 522 42 Cuts at: 4010 4374 4406 4466 5710 6845 7526 7717 826 Size: 364 32 60 1244 1135 681 191 1471 Fragments arranged by size: 1471 1253 1244 1135 681 522 521 364 342 219 191 164 121 60 42 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8362 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8362 SmaI CCC'GGG Cuts at: 1872 5689 1872 Size: 3817 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2823 4163 4257 4350 4908 5372 Size: 197 926 880 1340 94 93 558 464 Cuts at: 5372 6466 7367 7629 820 Size: 1094 901 262 1553 Fragments arranged by size: 1553 1340 1094 926 901 880 558 464 262 197 94 93 SnaBI TAC'GTA Cuts at: 493 6153 493 Size: 5660 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8362 SphI G_CATG'C Cuts at: 1132 3905 5720 1132 Size: 2773 1815 3774 Fragments arranged by size: 3774 2773 1815 SspI AAT'ATT Cuts at: 5 52 6286 5 Size: 47 6234 2081 Fragments arranged by size: 6234 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8362 StyI C'CwwG_G Cuts at: 513 1065 2104 3499 3921 4218 4452 4944 5149 Size: 552 1039 1395 422 297 234 492 205 Cuts at: 5149 5163 5801 513 Size: 14 638 3074 Fragments arranged by size: 3074 1395 1039 638 552 492 422 297 234 205 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3001 6389 6406 6559 6744 7189 325 Size: 1255 1421 3388 17 153 185 445 1498 Fragments arranged by size: 3388 1498 1421 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 4393 6608 Size: 274 80 33 51 1443 147 2269 2215 Cuts at: 6608 8320 96 Size: 1712 138 Fragments arranged by size: 2269 2215 1712 1443 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5159 5191 8322 8354 5159 Size: 32 3131 32 5167 Fragments arranged by size: 5167 3131 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3220 1316 Size: 1904 6458 XbaI T'CTAG_A Cuts at: 5698 5698 Size: 8362 XhoI C'TCGA_G Cuts at: 1943 4350 1943 Size: 2407 5955 XmaI C'CCGG_G Cuts at: 1870 5687 1870 Size: 3817 4545 XmnI GAAnn'nnTTC Cuts at: 4867 6491 4867 Size: 1624 6738 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6170 276 Size: 53 83 186 936 4636 2468 Fragments arranged by size: 4636 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BsiWI BstZ17I DraIII EcoRV FseI KasI NarI NruI PacI PfoI PmlI PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI