(Circular) (Six-base) MAPSORT of: pFN21AA0633.seq Check: 2817 from: 1 to: 8488 pFN21AA0633 8488 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3142 5501 3142 Size: 2359 6129 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2199 3825 6298 278 Size: 53 83 186 936 663 1626 2473 2468 Fragments arranged by size: 2473 2468 1626 936 663 186 83 53 AccI GT'mk_AC Cuts at: 1937 4889 5831 1937 Size: 2952 942 4594 Fragments arranged by size: 4594 2952 942 Acc65I G'GTAC_C Cuts at: 5809 5809 Size: 8488 AclI AA'CG_TT Cuts at: 1563 6615 6988 1563 Size: 5052 373 3063 Fragments arranged by size: 5052 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2270 2316 2442 4296 6551 7935 8203 1632 Size: 638 46 126 1854 2255 1384 268 1917 Fragments arranged by size: 2255 1917 1854 1384 638 268 126 46 AflII C'TTAA_G Cuts at: 820 1017 4106 820 Size: 197 3089 5202 Fragments arranged by size: 5202 3089 197 AflIII A'CryG_T Cuts at: 1203 2670 7387 8364 1203 Size: 1467 4717 977 1327 Fragments arranged by size: 4717 1467 1327 977 AgeI A'CCGG_T Cuts at: 4012 7319 4012 Size: 3307 5181 AhdI GACnn_n'nnGTC Cuts at: 7217 7217 Size: 8488 AleI CACnn'nnGTG Cuts at: 1166 4388 1166 Size: 3222 5266 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4479 1184 Size: 3295 5193 AlwNI CAG_nnn'CTG Cuts at: 3129 3157 5033 5756 6231 7803 3129 Size: 28 1876 723 475 1572 3814 Fragments arranged by size: 3814 1876 1572 723 475 28 ApaI G_GGCC'C Cuts at: 2137 2137 Size: 8488 ApaLI G'TGCA_C Cuts at: 2102 6545 7701 2102 Size: 4443 1156 2889 Fragments arranged by size: 4443 2889 1156 ApoI r'AATT_y Cuts at: 1493 2322 2744 4003 5285 5797 6023 6175 8284 Size: 829 422 1259 1282 512 226 152 2109 Cuts at: 8284 1493 Size: 1697 Fragments arranged by size: 2109 1697 1282 1259 829 512 422 226 152 AseI AT'TA_AT Cuts at: 160 1032 3455 7042 160 Size: 872 2423 3587 1606 Fragments arranged by size: 3587 2423 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8488 AvaI C'yCGr_G Cuts at: 1870 1943 2138 2651 5813 1870 Size: 73 195 513 3162 4545 Fragments arranged by size: 4545 3162 513 195 73 AvrII C'CTAG_G Cuts at: 4489 4853 4489 Size: 364 8124 BamHI G'GATC_C Cuts at: 5818 6185 5818 Size: 367 8121 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2452 3742 3886 4054 4068 Size: 325 217 627 665 1290 144 168 14 Cuts at: 4068 4540 4702 4787 5489 5690 5773 5809 7264 Size: 472 162 85 702 201 83 36 1455 Cuts at: 7264 618 Size: 1842 Fragments arranged by size: 1842 1455 1290 702 665 627 472 325 217 201 168 162 144 85 83 36 14 BanII G_rGCy'C Cuts at: 1391 2137 2522 2652 2667 2911 3148 3371 4364 Size: 746 385 130 15 244 237 223 993 Cuts at: 4364 4639 4849 5040 5535 5573 5807 1391 Size: 275 210 191 495 38 234 4072 Fragments arranged by size: 4072 993 746 495 385 275 244 237 234 223 210 191 130 38 15 BbeI G_GCGC'C Cuts at: 3746 3890 4706 3746 Size: 144 816 7528 Fragments arranged by size: 7528 816 144 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2322 3321 3453 3504 3522 4254 4938 Size: 971 423 999 132 51 18 732 684 Cuts at: 4938 5594 5598 928 Size: 656 4 3818 Fragments arranged by size: 3818 999 971 732 684 656 423 132 51 18 4 BbvCI CC'TCA_GC Cuts at: 2177 5696 2177 Size: 3519 4969 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3596 3630 5438 5472 6679 6713 1903 Size: 34 1659 34 1808 34 1207 34 3678 Fragments arranged by size: 3678 1808 1659 1207 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6380 7590 6380 Size: 1210 7278 BclI T'GATC_A Cuts at: 1548 1548 Size: 8488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 2218 7099 Size: 107 122 71 631 605 355 191 4881 Cuts at: 7099 136 Size: 1525 Fragments arranged by size: 4881 1525 631 605 355 191 122 107 71 BglII A'GATC_T Cuts at: 8483 8483 Size: 8488 BlpI GC'TnA_GC Cuts at: 3756 5026 5905 3756 Size: 1270 879 6339 Fragments arranged by size: 6339 1270 879 Bme1580I G_kGCm'C Cuts at: 1790 2106 2137 4395 4792 5484 5693 5778 6549 Size: 316 31 2258 397 692 209 85 771 Cuts at: 6549 7705 1790 Size: 1156 2573 Fragments arranged by size: 2573 2258 1156 771 692 397 316 209 85 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8488 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2543 3617 4786 Size: 416 53 83 399 24 1126 1074 1169 Cuts at: 4786 5208 6219 7177 442 Size: 422 1011 958 1753 Fragments arranged by size: 1753 1169 1126 1074 1011 958 422 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8488 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5041 5041 Size: 8488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2243 2519 2645 2904 3483 4336 5145 5519 Size: 1004 276 126 259 579 853 809 374 Cuts at: 5519 5659 7148 1239 Size: 140 1489 2579 Fragments arranged by size: 2579 1489 1004 853 809 579 374 276 259 140 126 Bpu10I CC'TnA_GC Cuts at: 2177 5565 5696 2177 Size: 3388 131 4969 Fragments arranged by size: 4969 3388 131 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3969 4559 6613 7478 7776 3969 Size: 590 2054 865 298 4681 Fragments arranged by size: 4681 2054 865 590 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3167 5299 7151 882 Size: 617 1668 2132 1852 2219 Fragments arranged by size: 2219 2132 1852 1668 617 BsaAI yAC'GTr Cuts at: 493 1204 2587 4534 6279 493 Size: 711 1383 1947 1745 2702 Fragments arranged by size: 2702 1947 1745 1383 711 BsaBI GATnn'nnATC Cuts at: 3870 8482 3870 Size: 4612 3876 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2196 3743 3822 3887 Size: 53 83 186 936 663 1547 79 65 Cuts at: 3887 4703 5733 6295 6677 7312 275 Size: 816 1030 562 382 635 1451 Fragments arranged by size: 1547 1451 1030 936 816 663 635 562 382 186 83 79 65 53 BsaWI w'CCGG_w Cuts at: 4012 4187 6921 7319 7593 7740 8151 4012 Size: 175 2734 398 274 147 411 4349 Fragments arranged by size: 4349 2734 411 398 274 175 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3164 3194 3524 3554 4907 Size: 30 165 30 1761 30 330 30 1353 Cuts at: 4907 4937 1178 Size: 30 4729 Fragments arranged by size: 4729 1761 1353 330 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2245 2585 2633 2904 2907 3092 3185 4800 Size: 1059 340 48 271 3 185 93 1615 Cuts at: 4800 5543 1186 Size: 743 4131 Fragments arranged by size: 4131 1615 1059 743 340 271 185 93 48 3 BseYI C'CCAG_C Cuts at: 2839 2857 3695 4210 4452 5261 7691 8187 2839 Size: 18 838 515 242 809 2430 496 3140 Fragments arranged by size: 3140 2430 838 809 515 496 242 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3788 4701 5315 5442 5643 1163 Size: 573 2052 913 614 127 201 4008 Fragments arranged by size: 4008 2052 913 614 573 201 127 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2043 3216 5938 6699 7727 1540 Size: 275 186 42 1173 2722 761 1028 2301 Fragments arranged by size: 2722 2301 1173 1028 761 275 186 42 BsiHKAI G_wGCw'C Cuts at: 2068 2106 2241 2586 2652 2667 3095 3762 4364 Size: 38 135 345 66 15 428 667 602 Cuts at: 4364 5535 6549 6634 7705 2068 Size: 1171 1014 85 1071 2851 Fragments arranged by size: 2851 1171 1071 1014 667 602 428 345 135 85 66 38 15 BsmI GAATG_Cn' Cuts at: 5999 6092 5999 Size: 93 8395 BsmBI CGTCTCn'nnnn_ Cuts at: 3259 3259 Size: 8488 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2106 2137 2241 2522 2586 2652 Size: 399 278 38 31 104 281 64 66 Cuts at: 2652 2667 2911 3095 3148 3371 3762 4364 4395 Size: 15 244 184 53 223 391 602 31 Cuts at: 4395 4639 4792 4849 5040 5484 5535 5573 5693 Size: 244 153 57 191 444 51 38 120 Cuts at: 5693 5778 5807 6549 6634 7705 1391 Size: 85 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 602 444 399 391 281 278 244 244 223 191 184 153 120 104 85 85 66 64 57 53 51 38 38 31 31 29 15 BspEI T'CCGG_A Cuts at: 8151 8151 Size: 8488 BspHI T'CATG_A Cuts at: 6377 8107 6377 Size: 1730 6758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2496 3142 3805 4912 5501 5843 844 Size: 1652 646 663 1107 589 342 3489 Fragments arranged by size: 3489 1652 1107 663 646 589 342 BsrBI CCG'CTC Cuts at: 1394 1823 5742 6375 1394 Size: 429 3919 633 3507 Fragments arranged by size: 3919 3507 633 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2607 2722 4617 6983 7157 65 Size: 1183 1359 115 1895 2366 174 1396 Fragments arranged by size: 2366 1895 1396 1359 1183 174 115 BsrFI r'CCGG_y Cuts at: 1700 1810 4012 4238 7132 7319 1700 Size: 110 2202 226 2894 187 2869 Fragments arranged by size: 2894 2869 2202 226 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8488 BssHII G'CGCG_C Cuts at: 1925 2017 5128 1925 Size: 92 3111 5285 Fragments arranged by size: 5285 3111 92 BssSI C'ACGA_G Cuts at: 2061 5783 6548 7560 2061 Size: 3722 765 1012 2989 Fragments arranged by size: 3722 2989 1012 765 BstAPI GCAn_nnn'nTGC Cuts at: 794 3129 5519 5561 794 Size: 2335 2390 42 3721 Fragments arranged by size: 3721 2390 2335 42 BstBI TT'CG_AA Cuts at: 3911 7292 3911 Size: 3381 5107 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8488 BstXI CCAn_nnnn'nTGG Cuts at: 2191 4065 4525 2191 Size: 1874 460 6154 Fragments arranged by size: 6154 1874 460 BstYI r'GATC_y Cuts at: 1972 5818 6185 6570 6587 8039 8125 8137 8483 Size: 3846 367 385 17 1452 86 12 346 Cuts at: 8483 1972 Size: 1977 Fragments arranged by size: 3846 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2448 2523 2597 5501 2448 Size: 75 74 2904 5435 Fragments arranged by size: 5435 2904 75 74 BtgI C'CryG_G Cuts at: 513 1065 2004 2190 2202 3559 4518 4822 5140 Size: 552 939 186 12 1357 959 304 318 Cuts at: 5140 5476 513 Size: 336 3525 Fragments arranged by size: 3525 1357 959 939 552 336 318 304 186 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2270 4171 487 Size: 683 56 1044 1901 4804 Fragments arranged by size: 4804 1901 1044 683 56 BtsI GCAGTG_nn' Cuts at: 3127 3328 3574 4845 5459 6004 6798 6818 3127 Size: 201 246 1271 614 545 794 20 4797 Fragments arranged by size: 4797 1271 794 614 545 246 201 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8453 DraI TTT'AAA Cuts at: 5792 5792 Size: 8488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7495 809 Size: 507 6179 1802 Fragments arranged by size: 6179 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 5255 5935 Size: 54 1425 50 275 18 198 3227 680 Cuts at: 5935 6824 8453 8 Size: 889 1629 43 Fragments arranged by size: 3227 1629 1425 889 680 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5935 1537 Size: 275 4123 4090 Fragments arranged by size: 4123 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 2418 2680 3083 3359 6418 1353 Size: 869 196 262 403 276 3059 3423 Fragments arranged by size: 3423 3059 869 403 276 262 196 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3182 7075 7449 7595 387 Size: 1064 138 1593 3893 374 146 1280 Fragments arranged by size: 3893 1593 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2650 2665 4362 5533 2650 Size: 15 1697 1171 5605 Fragments arranged by size: 5605 1697 1171 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2270 2316 2442 2519 2645 2904 Size: 393 611 27 46 126 77 126 259 Cuts at: 2904 3483 4296 4336 5145 5519 5659 6551 7148 Size: 579 813 40 809 374 140 892 597 Cuts at: 7148 7935 8203 1239 Size: 787 268 1524 Fragments arranged by size: 1524 892 813 809 787 611 597 579 393 374 268 259 140 126 126 77 46 40 27 EcoNI CCTnn'n_nnAGG Cuts at: 2919 4493 5101 5163 5504 2919 Size: 1574 608 62 341 5903 Fragments arranged by size: 5903 1574 608 341 62 EcoO109I rG'GnC_Cy Cuts at: 2133 2134 2379 3331 3356 4373 2133 Size: 1 245 952 25 1017 6248 Fragments arranged by size: 6248 1017 952 245 25 1 EcoRI G'AATT_C Cuts at: 4003 5797 6175 4003 Size: 1794 378 6316 Fragments arranged by size: 6316 1794 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3388 5794 3388 Size: 2406 6082 FspI TGC'GCA Cuts at: 6994 6994 Size: 8488 HaeII r_GCGC'y Cuts at: 1678 2177 2905 3011 3746 3890 4706 7635 1678 Size: 499 728 106 735 144 816 2929 2531 Fragments arranged by size: 2929 2531 816 735 728 499 144 106 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2044 2076 2936 2968 3164 Size: 32 135 32 472 32 860 32 196 Cuts at: 3164 3196 3239 3271 3524 3556 4393 4425 4733 Size: 32 43 32 253 32 837 32 308 Cuts at: 4733 4765 7129 7161 7203 7235 1373 Size: 32 2364 32 42 32 2626 Fragments arranged by size: 2626 2364 860 837 472 308 253 196 135 43 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5832 6078 669 Size: 988 281 3894 246 3079 Fragments arranged by size: 3894 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8488 HpaI GTT'AAC Cuts at: 6078 6078 Size: 8488 KasI G'GCGC_C Cuts at: 3742 3886 4702 3742 Size: 144 816 7528 Fragments arranged by size: 7528 816 144 KpnI G_GTAC'C Cuts at: 5813 5813 Size: 8488 MfeI C'AATT_G Cuts at: 6087 6087 Size: 8488 MluI A'CGCG_T Cuts at: 2670 8364 2670 Size: 5694 2794 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2868 3183 3594 3805 7602 7786 1305 Size: 1563 315 411 211 3797 184 2007 Fragments arranged by size: 3797 2007 1563 411 315 211 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5257 10 Size: 54 1425 343 3425 3241 Fragments arranged by size: 3425 3241 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4388 4523 4653 4674 4774 6446 6805 Size: 648 3222 135 130 21 100 1672 359 Cuts at: 6805 6964 518 Size: 159 2042 Fragments arranged by size: 3222 2042 1672 648 359 159 135 130 100 21 MspA1I CmG'CkG Cuts at: 1606 3192 5478 5487 5904 6581 7729 7974 1606 Size: 1586 2286 9 417 677 1148 245 2120 Fragments arranged by size: 2286 2120 1586 1148 677 417 245 9 NarI GG'CG_CC Cuts at: 3743 3887 4703 3743 Size: 144 816 7528 Fragments arranged by size: 7528 816 144 NcoI C'CATG_G Cuts at: 513 1065 2004 2190 3559 4518 513 Size: 552 939 186 1369 959 4483 Fragments arranged by size: 4483 1369 959 939 552 186 NdeI CA'TA_TG Cuts at: 387 387 Size: 8488 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8488 NotI GC'GGCC_GC Cuts at: 5935 5935 Size: 8488 NspI r_CATG'y Cuts at: 1132 1229 2102 5846 7391 1132 Size: 97 873 3744 1545 2229 Fragments arranged by size: 3744 2229 1545 873 97 PasI CC'CwG_GG Cuts at: 1348 3112 3891 4088 1348 Size: 1764 779 197 5748 Fragments arranged by size: 5748 1764 779 197 PciI A'CATG_T Cuts at: 7387 7387 Size: 8488 PflMI CCAn_nnn'nTGG Cuts at: 1108 2078 4606 1108 Size: 970 2528 4990 Fragments arranged by size: 4990 2528 970 PfoI T'CCnGG_A Cuts at: 2077 5161 2077 Size: 3084 5404 PmeI GTTT'AAAC Cuts at: 5792 5792 Size: 8488 PmlI CAC'GTG Cuts at: 2587 4534 2587 Size: 1947 6541 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6557 6589 8070 8102 6557 Size: 32 1481 32 6943 Fragments arranged by size: 6943 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2379 3331 3356 4373 2379 Size: 952 25 1017 6494 Fragments arranged by size: 6494 1017 952 25 PshAI GACnn'nnGTC Cuts at: 1613 3354 5598 1613 Size: 1741 2244 4503 Fragments arranged by size: 4503 2244 1741 PsiI TTA'TAA Cuts at: 6058 6058 Size: 8488 PspOMI G'GGCC_C Cuts at: 2133 2133 Size: 8488 PstI C_TGCA'G Cuts at: 830 3134 3323 5102 5498 5840 830 Size: 2304 189 1779 396 342 3478 Fragments arranged by size: 3478 2304 1779 396 342 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8488 PvuII CAG'CTG Cuts at: 3192 3192 Size: 8488 SacI G_AGCT'C Cuts at: 2652 2667 4364 5535 2652 Size: 15 1697 1171 5605 Fragments arranged by size: 5605 1697 1171 15 SacII CC_GC'GG Cuts at: 5479 5479 Size: 8488 SalI G'TCGA_C Cuts at: 1936 5830 1936 Size: 3894 4594 SanDI GG'GwC_CC Cuts at: 2379 3356 4373 2379 Size: 977 1017 6494 Fragments arranged by size: 6494 1017 977 SapI GCTCTTCn'nnn_ Cuts at: 3083 3359 3083 Size: 276 8212 SbfI CC_TGCA'GG Cuts at: 5840 5840 Size: 8488 ScaI AGT'ACT Cuts at: 6736 8448 6736 Size: 1712 6776 SfcI C'TryA_G Cuts at: 826 1045 3130 3319 4099 5098 5226 5494 5836 Size: 219 2085 189 780 999 128 268 342 Cuts at: 5836 6971 7652 7843 826 Size: 1135 681 191 1471 Fragments arranged by size: 2085 1471 1135 999 780 681 342 268 219 191 189 128 SfoI GGC'GCC Cuts at: 3744 3888 4704 3744 Size: 144 816 7528 Fragments arranged by size: 7528 816 144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8488 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8488 SmaI CCC'GGG Cuts at: 1872 5815 1872 Size: 3943 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3984 4106 4574 6592 7493 7755 Size: 197 926 2041 122 468 2018 901 262 Cuts at: 7755 820 Size: 1553 Fragments arranged by size: 2041 2018 1553 926 901 468 262 197 122 SnaBI TAC'GTA Cuts at: 493 6279 493 Size: 5786 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8488 SphI G_CATG'C Cuts at: 1132 5846 1132 Size: 4714 3774 SspI AAT'ATT Cuts at: 5 52 2542 6412 5 Size: 47 2490 3870 2081 Fragments arranged by size: 3870 2490 2081 47 StuI AGG'CCT Cuts at: 1517 5096 1517 Size: 3579 4909 StyI C'CwwG_G Cuts at: 513 1065 2004 2190 3559 4050 4489 4518 4853 Size: 552 939 186 1369 491 439 29 335 Cuts at: 4853 5596 5663 5927 513 Size: 743 67 264 3074 Fragments arranged by size: 3074 1369 939 743 552 491 439 335 264 186 67 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2316 3230 5275 6515 6532 6685 6870 Size: 1255 736 914 2045 1240 17 153 185 Cuts at: 6870 7315 325 Size: 445 1498 Fragments arranged by size: 2045 1498 1255 1240 914 736 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6734 8446 96 Size: 274 80 33 51 1443 4757 1712 138 Fragments arranged by size: 4757 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3797 3825 3829 3857 4771 4803 4907 4939 8448 Size: 28 4 28 914 32 104 32 3509 Cuts at: 8448 8480 3797 Size: 32 3805 Fragments arranged by size: 3805 3509 914 104 32 32 32 28 28 4 Tth111I GACn'n_nGTC Cuts at: 1316 3178 3251 1316 Size: 1862 73 6553 Fragments arranged by size: 6553 1862 73 XbaI T'CTAG_A Cuts at: 5824 5824 Size: 8488 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3335 4522 4603 3335 Size: 1187 81 7220 Fragments arranged by size: 7220 1187 81 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8488 XmaI C'CCGG_G Cuts at: 1870 5813 1870 Size: 3943 4545 XmnI GAAnn'nnTTC Cuts at: 2415 5285 6617 2415 Size: 2870 1332 4286 Fragments arranged by size: 4286 2870 1332 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2197 3823 6296 276 Size: 53 83 186 936 663 1626 2473 2468 Fragments arranged by size: 2473 2468 1626 936 663 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SexAI SfiI SrfI SwaI