Restriction map for
pFN21AB8829
(Circular) (Six-base) MAPSORT of: pFN21AB8829.seq Check: 5896 from: 1 to: 6016
pFN21AB8829 6016 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3359 1937
Size: 1422 4594
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2491 2995 4079 5463 5731 1632
Size: 859 504 1084 1384 268 1917
Fragments arranged by size:
1917 1384 1084 859 504 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 2126 4915 5892 1203
Size: 923 2789 977 1327
Fragments arranged by size:
2789 1327 977 923
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 4745 4745
Size: 6016
AleI CACnn'nnGTG
Cuts at: 1166 2200 3054 1166
Size: 1034 854 4128
Fragments arranged by size:
4128 1034 854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6016
AlwNI CAG_nnn'CTG
Cuts at: 3279 3759 5331 3279
Size: 480 1572 3964
Fragments arranged by size:
3964 1572 480
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 2210 3213 3325 3551 3703 5812 1493
Size: 717 1003 112 226 152 2109 1697
Fragments arranged by size:
2109 1697 1003 717 226 152 112
AseI AT'TA_AT
Cuts at: 160 1032 3309 4570 160
Size: 872 2277 1261 1606
Fragments arranged by size:
2277 1606 1261 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 3341 1870
Size: 73 1398 4545
Fragments arranged by size:
4545 1398 73
BamHI G'GATC_C
Cuts at: 3346 3713 3346
Size: 367 5649
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3337 4792 618
Size: 325 217 627 1550 1455 1842
Fragments arranged by size:
1842 1550 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3335 1391
Size: 1944 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2079 2788 928
Size: 971 180 709 4156
Fragments arranged by size:
4156 971 709 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4207 4241 1903
Size: 34 2270 34 3678
Fragments arranged by size:
3678 2270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3908 5118 3908
Size: 1210 4806
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4627 136
Size: 107 122 71 631 605 2955 1525
Fragments arranged by size:
2955 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3014 6011 3014
Size: 2997 3019
BlpI GC'TnA_GC
Cuts at: 3433 3433
Size: 6016
Bme1580I G_kGCm'C
Cuts at: 1790 4077 5233 1790
Size: 2287 1156 2573
Fragments arranged by size:
2573 2287 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6016
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2559 2679 2847
Size: 416 53 83 399 24 1142 120 168
Cuts at: 2847 3747 4705 442
Size: 900 958 1753
Fragments arranged by size:
1753 1142 958 900 416 399 168 120
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2033 2033
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2121 2487 2823 2907 2991 3159 3243 4676
Size: 882 366 336 84 84 168 84 1433
Cuts at: 4676 1239
Size: 2579
Fragments arranged by size:
2579 1433 882 366 336 168 84 84
84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2275 4141 5006 5304 2275
Size: 1866 865 298 2987
Fragments arranged by size:
2987 1866 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4679 882
Size: 617 3180 2219
Fragments arranged by size:
3180 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3807 493
Size: 711 2603 2702
Fragments arranged by size:
2702 2603 711
BsaBI GATnn'nnATC
Cuts at: 2152 6010 2152
Size: 3858 2158
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3823 4205 4840 275
Size: 53 83 186 936 2290 382 635 1451
Fragments arranged by size:
2290 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2068 4449 4847 5121 5268 5679 2068
Size: 2381 398 274 147 411 2405
Fragments arranged by size:
2405 2381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5791
Fragments arranged by size:
5791 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6016
BseYI C'CCAG_C
Cuts at: 5219 5715 5219
Size: 496 5520
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2049 1163
Size: 573 313 5130
Fragments arranged by size:
5130 573 313
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4077 4162 5233 4077
Size: 85 1071 4860
Fragments arranged by size:
4860 1071 85
BsmI GAATG_Cn'
Cuts at: 2355 2799 3527 3620 2355
Size: 444 728 93 4751
Fragments arranged by size:
4751 728 444 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3335 4077 4162 5233 1391
Size: 399 1545 742 85 1071 2174
Fragments arranged by size:
2174 1545 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2068 5679 2068
Size: 3611 2405
BspHI T'CATG_A
Cuts at: 2953 3905 5635 2953
Size: 952 1730 3334
Fragments arranged by size:
3334 1730 952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3371 844
Size: 2527 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3903 1394
Size: 429 2080 3507
Fragments arranged by size:
3507 2080 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2918 3002 3086 4511 4685 65
Size: 1183 1670 84 84 1425 174 1396
Fragments arranged by size:
1670 1425 1396 1183 174 84 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 4660 4847 1700
Size: 110 2850 187 2869
Fragments arranged by size:
2869 2850 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6016
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6016
BstBI TT'CG_AA
Cuts at: 2275 2359 2527 2779 3031 4820 2275
Size: 84 168 252 252 1789 3471
Fragments arranged by size:
3471 1789 252 252 168 84
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstYI r'GATC_y
Cuts at: 1972 3014 3346 3713 4098 4115 5567 5653 5665
Size: 1042 332 367 385 17 1452 86 12
Cuts at: 5665 6011 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1042 385 367 346 332 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4525
Fragments arranged by size:
4525 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5277
Fragments arranged by size:
5277 683 56
BtsI GCAGTG_nn'
Cuts at: 2266 3532 4326 4346 2266
Size: 1266 794 20 3936
Fragments arranged by size:
3936 1266 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2896 2931 529
Size: 35 2332 35 3614
Fragments arranged by size:
3614 2332 35 35
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3463 4352 5981
Size: 54 1425 50 275 18 1633 889 1629
Cuts at: 5981 8
Size: 43
Fragments arranged by size:
1633 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 3946 1353
Size: 747 1846 3423
Fragments arranged by size:
3423 1846 747
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4603 4977 5123 387
Size: 1064 138 3014 374 146 1280
Fragments arranged by size:
3014 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2487 2491 2823 2907 2991 2995
Size: 393 489 366 4 332 84 84 4
Cuts at: 2995 3159 3243 4079 4676 5463 5731 1239
Size: 164 84 836 597 787 268 1524
Fragments arranged by size:
1524 836 787 597 489 393 366 332
268 164 84 84 84 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2161 2161
Size: 6016
EcoRI G'AATT_C
Cuts at: 3213 3325 3703 3213
Size: 112 378 5526
Fragments arranged by size:
5526 378 112
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 5163 1678
Size: 3485 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4657 4689 4731 4763 1373
Size: 32 135 32 3085 32 42 32 2626
Fragments arranged by size:
3085 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3360 3606 669
Size: 988 281 1422 246 3079
Fragments arranged by size:
3079 1422 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2773 748
Size: 2025 3991
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 2677 3615 2677
Size: 938 5078
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5130 5314 1305
Size: 3825 184 2007
Fragments arranged by size:
3825 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4194
Fragments arranged by size:
4194 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2200 2566 2700 2792 3054 3120 3974
Size: 648 1034 366 134 92 262 66 854
Cuts at: 3974 4333 4492 518
Size: 359 159 2042
Fragments arranged by size:
2042 1034 854 648 366 359 262 159
134 92 66
MspA1I CmG'CkG
Cuts at: 1606 3432 4109 5257 5502 1606
Size: 1826 677 1148 245 2120
Fragments arranged by size:
2120 1826 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4525
Fragments arranged by size:
4525 939 552
NdeI CA'TA_TG
Cuts at: 387 2563 387
Size: 2176 3840
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 2080 2130 3374 4919 1132
Size: 97 851 50 1244 1545 2229
Fragments arranged by size:
2229 1545 1244 851 97 50
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6016
PciI A'CATG_T
Cuts at: 2126 4915 2126
Size: 2789 3227
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6016
PfoI T'CCnGG_A
Cuts at: 2098 2098
Size: 6016
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 5598 5630 4085
Size: 32 1481 32 4471
Fragments arranged by size:
4471 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 2397 2771 3586 2397
Size: 374 815 4827
Fragments arranged by size:
4827 815 374
PstI C_TGCA'G
Cuts at: 830 3368 830
Size: 2538 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 3364 4499 5180 5371 826
Size: 219 2319 1135 681 191 1471
Fragments arranged by size:
2319 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 3343 1872
Size: 1471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2254 4120 5021 5283 820
Size: 197 926 311 1866 901 262 1553
Fragments arranged by size:
1866 1553 926 901 311 262 197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 3374 1132
Size: 2242 3774
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 2290 2812 1517
Size: 773 522 4721
Fragments arranged by size:
4721 773 522
StyI C'CwwG_G
Cuts at: 513 1065 2004 2421 3455 513
Size: 552 939 417 1034 3074
Fragments arranged by size:
3074 1034 939 552 417
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2075 2976 4043 4060 4213 4398 4843
Size: 1255 495 901 1067 17 153 185 445
Cuts at: 4843 325
Size: 1498
Fragments arranged by size:
1498 1255 1067 901 495 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3024 4262 5974
Size: 274 80 33 51 1443 1047 1238 1712
Cuts at: 5974 96
Size: 138
Fragments arranged by size:
1712 1443 1238 1047 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2050 2082 5976 6008 2050
Size: 32 3894 32 2058
Fragments arranged by size:
3894 2058 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6016
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 3341 1870
Size: 1471 4545
XmnI GAAnn'nnTTC
Cuts at: 2588 2672 2924 3008 3176 3213 3260 4145 2588
Size: 84 252 84 168 37 47 885 4459
Fragments arranged by size:
4459 885 252 168 84 84 47 37
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI