(Circular) (Six-base) MAPSORT of: pFN21AA0623.seq Check: 3833 from: 1 to: 7813 pFN21AA0623 7813 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5623 278 Size: 53 83 186 936 4087 2468 Fragments arranged by size: 4087 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4054 5156 1937 Size: 2117 1102 4594 Fragments arranged by size: 4594 2117 1102 Acc65I G'GTAC_C Cuts at: 3449 5134 3449 Size: 1685 6128 AclI AA'CG_TT Cuts at: 1563 5940 6313 1563 Size: 4377 373 3063 Fragments arranged by size: 4377 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2177 2744 3912 5876 7260 7528 1632 Size: 545 567 1168 1964 1384 268 1917 Fragments arranged by size: 1964 1917 1384 1168 567 545 268 AflII C'TTAA_G Cuts at: 820 1017 2668 820 Size: 197 1651 5965 Fragments arranged by size: 5965 1651 197 AflIII A'CryG_T Cuts at: 1203 4227 6712 7689 1203 Size: 3024 2485 977 1327 Fragments arranged by size: 3024 2485 1327 977 AgeI A'CCGG_T Cuts at: 6644 6644 Size: 7813 AhdI GACnn_n'nnGTC Cuts at: 6542 6542 Size: 7813 AleI CACnn'nnGTG Cuts at: 1166 3099 4751 1166 Size: 1933 1652 4228 Fragments arranged by size: 4228 1933 1652 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2364 4820 1184 Size: 1180 2456 4177 Fragments arranged by size: 4177 2456 1180 AlwNI CAG_nnn'CTG Cuts at: 5556 7128 5556 Size: 1572 6241 ApaI G_GGCC'C Cuts at: 4263 4263 Size: 7813 ApaLI G'TGCA_C Cuts at: 4564 5870 7026 4564 Size: 1306 1156 5351 Fragments arranged by size: 5351 1306 1156 ApoI r'AATT_y Cuts at: 1493 3143 4790 5122 5348 5500 7609 1493 Size: 1650 1647 332 226 152 2109 1697 Fragments arranged by size: 2109 1697 1650 1647 332 226 152 AseI AT'TA_AT Cuts at: 160 1032 2127 6367 160 Size: 872 1095 4240 1606 Fragments arranged by size: 4240 1606 1095 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7813 AvaI C'yCGr_G Cuts at: 1870 1943 5138 1870 Size: 73 3195 4545 Fragments arranged by size: 4545 3195 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3302 3335 3302 Size: 33 7780 BamHI G'GATC_C Cuts at: 2531 5143 5510 2531 Size: 2612 367 4834 Fragments arranged by size: 4834 2612 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2085 3070 3449 4135 5134 Size: 325 217 627 298 985 379 686 999 Cuts at: 5134 6589 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 999 985 686 627 379 325 298 217 BanII G_rGCy'C Cuts at: 1391 4263 4280 4419 4742 5132 1391 Size: 2872 17 139 323 390 4072 Fragments arranged by size: 4072 2872 390 323 139 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2065 2325 3408 5083 928 Size: 971 166 260 1083 1675 3658 Fragments arranged by size: 3658 1675 1083 971 260 166 BbvCI CC'TCA_GC Cuts at: 3693 3693 Size: 7813 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6004 6038 1903 Size: 34 4067 34 3678 Fragments arranged by size: 4067 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5705 6915 5705 Size: 1210 6603 BclI T'GATC_A Cuts at: 1548 1548 Size: 7813 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3336 3703 6424 Size: 107 122 71 631 605 1664 367 2721 Cuts at: 6424 136 Size: 1525 Fragments arranged by size: 2721 1664 1525 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 2395 3299 4001 7808 2395 Size: 904 702 3807 2400 Fragments arranged by size: 3807 2400 904 702 BlpI GC'TnA_GC Cuts at: 4628 5230 4628 Size: 602 7211 Bme1580I G_kGCm'C Cuts at: 1790 2859 3659 3934 4263 4568 5874 7030 1790 Size: 1069 800 275 329 305 1306 1156 2573 Fragments arranged by size: 2573 1306 1156 1069 800 329 305 275 BmgBI CAC'GTC Cuts at: 1318 3131 1318 Size: 1813 6000 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2840 3359 3420 Size: 416 53 83 399 24 1423 519 61 Cuts at: 3420 3678 3693 4648 5544 6502 442 Size: 258 15 955 896 958 1753 Fragments arranged by size: 1753 1423 958 955 896 519 416 399 258 83 61 53 24 15 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7813 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3490 3516 4332 6473 1239 Size: 2251 26 816 2141 2579 Fragments arranged by size: 2579 2251 2141 816 26 Bpu10I CC'TnA_GC Cuts at: 2583 3271 3459 3693 4557 2583 Size: 688 188 234 864 5839 Fragments arranged by size: 5839 864 688 234 188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2646 2837 3828 5938 6803 7101 2646 Size: 191 991 2110 865 298 3358 Fragments arranged by size: 3358 2110 991 865 298 191 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2271 4194 6476 882 Size: 617 772 1923 2282 2219 Fragments arranged by size: 2282 2219 1923 772 617 BsaAI yAC'GTr Cuts at: 493 1204 4381 5604 493 Size: 711 3177 1223 2702 Fragments arranged by size: 3177 2702 1223 711 BsaBI GATnn'nnATC Cuts at: 7807 7807 Size: 7813 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5620 6002 6637 275 Size: 53 83 186 936 4087 382 635 1451 Fragments arranged by size: 4087 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6246 6644 6918 7065 7476 6246 Size: 398 274 147 411 6583 Fragments arranged by size: 6583 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3805 3835 1178 Size: 30 165 30 2402 30 5156 Fragments arranged by size: 5156 2402 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3511 4182 4463 1186 Size: 2325 671 281 4536 Fragments arranged by size: 4536 2325 671 281 BseYI C'CCAG_C Cuts at: 3674 4262 4367 4403 7016 7512 3674 Size: 588 105 36 2613 496 3975 Fragments arranged by size: 3975 2613 588 496 105 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 1163 Size: 573 618 6622 Fragments arranged by size: 6622 618 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3350 5263 6024 7052 1540 Size: 275 186 1349 1913 761 1028 2301 Fragments arranged by size: 2301 1913 1349 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4568 5874 5959 7030 4568 Size: 1306 85 1071 5351 Fragments arranged by size: 5351 1306 1071 85 BsmI GAATG_Cn' Cuts at: 5324 5417 5324 Size: 93 7720 BsmBI CGTCTCn'nnnn_ Cuts at: 4445 4445 Size: 7813 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2859 3659 3934 4263 4280 4419 4568 Size: 399 1069 800 275 329 17 139 149 Cuts at: 4568 4742 5132 5874 5959 7030 1391 Size: 174 390 742 85 1071 2174 Fragments arranged by size: 2174 1071 1069 800 742 399 390 329 275 174 149 139 85 17 BspEI T'CCGG_A Cuts at: 7476 7476 Size: 7813 BspHI T'CATG_A Cuts at: 5702 7432 5702 Size: 1730 6083 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4136 5168 844 Size: 3292 1032 3489 Fragments arranged by size: 3489 3292 1032 BsrBI CCG'CTC Cuts at: 1394 1823 4465 5700 1394 Size: 429 2642 1235 3507 Fragments arranged by size: 3507 2642 1235 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2275 3934 6308 6482 65 Size: 1183 1027 1659 2374 174 1396 Fragments arranged by size: 2374 1659 1396 1183 1027 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6457 6644 1700 Size: 110 4647 187 2869 Fragments arranged by size: 4647 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2541 3283 4665 96 Size: 2445 742 1382 3244 Fragments arranged by size: 3244 2445 1382 742 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7813 BssSI C'ACGA_G Cuts at: 2045 5873 6885 2045 Size: 3828 1012 2973 Fragments arranged by size: 3828 2973 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4142 794 Size: 3348 4465 BstBI TT'CG_AA Cuts at: 4237 6617 4237 Size: 2380 5433 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7813 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3334 3739 2011 Size: 1323 405 6085 Fragments arranged by size: 6085 1323 405 BstYI r'GATC_y Cuts at: 1972 2041 2395 2531 3299 4001 4590 5143 5510 Size: 69 354 136 768 702 589 553 367 Cuts at: 5510 5895 5912 7364 7450 7462 7808 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 768 702 589 553 385 367 354 346 136 86 69 17 12 Bsu36I CC'TnA_GG Cuts at: 2553 3492 3523 2553 Size: 939 31 6843 Fragments arranged by size: 6843 939 31 BtgI C'CryG_G Cuts at: 513 1065 2004 2067 3333 3752 3920 5109 513 Size: 552 939 63 1266 419 168 1189 3217 Fragments arranged by size: 3217 1266 1189 939 552 419 168 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7074 Fragments arranged by size: 7074 683 56 BtsI GCAGTG_nn' Cuts at: 3170 3312 3996 5329 6123 6143 3170 Size: 142 684 1333 794 20 4840 Fragments arranged by size: 4840 1333 794 684 142 20 ClaI AT'CG_AT Cuts at: 1554 4834 1554 Size: 3280 4533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4236 4271 529 Size: 35 3672 35 4071 Fragments arranged by size: 4071 3672 35 35 DraI TTT'AAA Cuts at: 3775 4080 5117 3775 Size: 305 1037 6471 Fragments arranged by size: 6471 1037 305 DraIII CAC_nnn'GTG Cuts at: 2528 4202 4518 2528 Size: 1674 316 5823 Fragments arranged by size: 5823 1674 316 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6820 809 Size: 507 5504 1802 Fragments arranged by size: 5504 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3347 4297 5260 Size: 54 1425 50 275 18 1517 950 963 Cuts at: 5260 6149 7778 8 Size: 889 1629 43 Fragments arranged by size: 1629 1517 1425 963 950 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3347 5260 1537 Size: 275 1535 1913 4090 Fragments arranged by size: 4090 1913 1535 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3046 4181 4345 5743 1353 Size: 1693 1135 164 1398 3423 Fragments arranged by size: 3423 1693 1398 1135 164 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6400 6774 6920 387 Size: 1064 138 4811 374 146 1280 Fragments arranged by size: 4811 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2744 3490 3516 3912 4332 5876 Size: 393 545 567 746 26 396 420 1544 Cuts at: 5876 6473 7260 7528 1239 Size: 597 787 268 1524 Fragments arranged by size: 1544 1524 787 746 597 567 545 420 396 393 268 26 EcoNI CCTnn'n_nnAGG Cuts at: 4143 4326 4143 Size: 183 7630 EcoO109I rG'GnC_Cy Cuts at: 2058 2984 3744 4259 4314 5026 2058 Size: 926 760 515 55 712 4845 Fragments arranged by size: 4845 926 760 712 515 55 EcoRI G'AATT_C Cuts at: 3143 5122 5500 3143 Size: 1979 378 5456 Fragments arranged by size: 5456 1979 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2608 2608 Size: 7813 FspI TGC'GCA Cuts at: 4808 6319 4808 Size: 1511 6302 HaeII r_GCGC'y Cuts at: 1678 3591 5090 6960 1678 Size: 1913 1499 1870 2531 Fragments arranged by size: 2531 1913 1870 1499 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3387 3419 3805 3837 6454 Size: 32 135 32 1815 32 386 32 2617 Cuts at: 6454 6486 6528 6560 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2617 1815 386 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4055 5157 5403 669 Size: 988 281 2117 1102 246 3079 Fragments arranged by size: 3079 2117 1102 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7813 HpaI GTT'AAC Cuts at: 5403 5403 Size: 7813 KpnI G_GTAC'C Cuts at: 3453 5138 3453 Size: 1685 6128 MfeI C'AATT_G Cuts at: 5412 5412 Size: 7813 MluI A'CGCG_T Cuts at: 7689 7689 Size: 7813 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2611 3345 3373 3879 6927 7111 1305 Size: 1306 734 28 506 3048 184 2007 Fragments arranged by size: 3048 2007 1306 734 506 184 28 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5991 Fragments arranged by size: 5991 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2577 3099 3925 4494 4751 4802 Size: 648 843 568 522 826 569 257 51 Cuts at: 4802 5771 6130 6289 518 Size: 969 359 159 2042 Fragments arranged by size: 2042 969 843 826 648 569 568 522 359 257 159 51 MspA1I CmG'CkG Cuts at: 1606 3967 4627 4657 4684 4751 5229 5906 7054 Size: 2361 660 30 27 67 478 677 1148 Cuts at: 7054 7299 1606 Size: 245 2120 Fragments arranged by size: 2361 2120 1148 677 660 478 245 67 30 27 NcoI C'CATG_G Cuts at: 513 1065 2004 3333 513 Size: 552 939 1329 4993 Fragments arranged by size: 4993 1329 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7813 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7813 NotI GC'GGCC_GC Cuts at: 5260 5260 Size: 7813 NspI r_CATG'y Cuts at: 1132 1229 4231 5171 6716 1132 Size: 97 3002 940 1545 2229 Fragments arranged by size: 3002 2229 1545 940 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7813 PciI A'CATG_T Cuts at: 4227 6712 4227 Size: 2485 5328 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7813 PfoI T'CCnGG_A Cuts at: 3506 4348 4593 4727 3506 Size: 842 245 134 6592 Fragments arranged by size: 6592 842 245 134 PmeI GTTT'AAAC Cuts at: 5117 5117 Size: 7813 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5882 5914 7395 7427 5882 Size: 32 1481 32 6268 Fragments arranged by size: 6268 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2984 3744 4314 5026 2984 Size: 760 570 712 5771 Fragments arranged by size: 5771 760 712 570 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7813 PsiI TTA'TAA Cuts at: 5383 5383 Size: 7813 PspOMI G'GGCC_C Cuts at: 4259 4259 Size: 7813 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4905 4937 4905 Size: 32 7781 PstI C_TGCA'G Cuts at: 830 3307 3585 4147 4888 4933 5035 5165 830 Size: 2477 278 562 741 45 102 130 3478 Fragments arranged by size: 3478 2477 741 562 278 130 102 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7813 PvuII CAG'CTG Cuts at: 4627 4657 4684 4751 4627 Size: 30 27 67 7689 Fragments arranged by size: 7689 67 30 27 SalI G'TCGA_C Cuts at: 1936 4053 5155 1936 Size: 2117 1102 4594 Fragments arranged by size: 4594 2117 1102 SanDI GG'GwC_CC Cuts at: 4314 4314 Size: 7813 SapI GCTCTTCn'nnn_ Cuts at: 4345 4345 Size: 7813 SbfI CC_TGCA'GG Cuts at: 4147 5165 4147 Size: 1018 6795 ScaI AGT'ACT Cuts at: 3025 6061 7773 3025 Size: 3036 1712 3065 Fragments arranged by size: 3065 3036 1712 SfcI C'TryA_G Cuts at: 826 1045 3303 3581 4143 4182 4884 4911 4929 Size: 219 2258 278 562 39 702 27 18 Cuts at: 4929 5021 5031 5161 6296 6977 7168 826 Size: 92 10 130 1135 681 191 1471 Fragments arranged by size: 2258 1471 1135 702 681 562 278 219 191 130 92 39 27 18 10 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7813 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7813 SmaI CCC'GGG Cuts at: 1872 5140 1872 Size: 3268 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2661 2668 2816 3843 5917 6818 Size: 197 926 718 7 148 1027 2074 901 Cuts at: 6818 7080 820 Size: 262 1553 Fragments arranged by size: 2074 1553 1027 926 901 718 262 197 148 7 SnaBI TAC'GTA Cuts at: 493 5604 493 Size: 5111 2702 SpeI A'CTAG_T Cuts at: 152 3026 152 Size: 2874 4939 SphI G_CATG'C Cuts at: 1132 5171 1132 Size: 4039 3774 SspI AAT'ATT Cuts at: 5 52 2206 5737 5 Size: 47 2154 3531 2081 Fragments arranged by size: 3531 2154 2081 47 StuI AGG'CCT Cuts at: 1517 3424 5007 1517 Size: 1907 1583 4323 Fragments arranged by size: 4323 1907 1583 StyI C'CwwG_G Cuts at: 513 1065 2004 3333 3715 3867 4164 5252 513 Size: 552 939 1329 382 152 297 1088 3074 Fragments arranged by size: 3074 1329 1088 939 552 382 297 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2006 4973 5840 5857 6010 6195 6640 Size: 1255 426 2967 867 17 153 185 445 Cuts at: 6640 325 Size: 1498 Fragments arranged by size: 2967 1498 1255 867 445 426 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2028 2541 3023 Size: 274 80 33 51 1443 51 513 482 Cuts at: 3023 3283 4665 6059 7771 96 Size: 260 1382 1394 1712 138 Fragments arranged by size: 1712 1443 1394 1382 513 482 274 260 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2299 2331 3728 3760 7773 7805 2299 Size: 32 1397 32 4013 32 2307 Fragments arranged by size: 4013 2307 1397 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7813 XbaI T'CTAG_A Cuts at: 5149 5149 Size: 7813 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2627 3671 3736 2011 Size: 616 1044 65 6088 Fragments arranged by size: 6088 1044 616 65 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7813 XmaI C'CCGG_G Cuts at: 1870 5138 1870 Size: 3268 4545 XmnI GAAnn'nnTTC Cuts at: 4835 4920 5942 4835 Size: 85 1022 6706 Fragments arranged by size: 6706 1022 85 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5621 276 Size: 53 83 186 936 4087 2468 Fragments arranged by size: 4087 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI