Restriction map for
pFN21AA0606
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# NO CUT ENZYMES ( 73 enzymes) #
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AatII Acc65I AccI AclI AgeI
ApaI AscI AseI AsnI Asp718I
AvaI BciVI BsaBI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BsrBI
BsrGI BssHII Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
FseI KpnI MamI MfeI MluI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 775 GAATTT [GA]^AATT[CT]
AfaI 2672 GTAC GT^AC
AflII 336 CTTAAG C^TTAAG
Alw44I 2130 GTGCAC G^TGCAC
ApaLI 2130 GTGCAC G^TGCAC
ApoI 775 GAATTT [GA]^AATT[CT]
AspI 2469 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2630 TGCGCA TGC^GCA
AvrII 2574 CCTAGG C^CTAGG
BalI 1236 TGGCCA TGG^CCA
BbeI 3320 GGCGCC GGCGC^C
BbrPI 2530 CACGTG CAC^GTG
BbsI 3130 GTCTTC GAAGAC(2/6)
BbvCI 3544 CCTCAGC CCTCAGC(-5/-2)
BcgI 2035 GCAGCCCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 336 CTTAAG C^TTAAG
BglII 2380 AGATCT A^GATCT
BlnI 2574 CCTAGG C^CTAGG
BpuAI 3130 GTCTTC GAAGAC(2/6)
BsaAI 2530 CACGTG [CT]AC^GT[GA]
BsmBI 1958 GAGACG CGTCTC(1/5)
BspLU11I 894 ACATGT A^CATGT
BsrDI 632 GCAATG GCAATG(2/0)
BsrFI 2770 GCCGGT [GA]^CCGG[CT]
BssSI 396 CTCGTG CACGAG(-5/-1)
BstBI 3398 TTCGAA TT^CGAA
BstXI 1975 CCAGCTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2770 GCCGGT [GA]^CCGG[CT]
DraI 462 TTTAAA TTT^AAA
DraII 160 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 1236 TGGCCA [CT]^GGCC[GA]
EcoO109I 160 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 208 ATGCAT ATGCA^T
FspI 2630 TGCGCA TGC^GCA
HpaI 1557 GTTAAC GTT^AAC
KasI 3320 GGCGCC G^GCGCC
KspI 3565 CCGCGG CCGC^GG
MluNI 1236 TGGCCA TGG^CCA
MscI 1236 TGGCCA TGG^CCA
NarI 3320 GGCGCC GG^CGCC
NsiI 208 ATGCAT ATGCA^T
NspV 3398 TTCGAA TT^CGAA
PflFI 2469 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 220 CCACTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2530 CACGTG CAC^GTG
PmlI 2530 CACGTG CAC^GTG
PpuMI 160 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 2672 GTAC GT^AC
SacII 3565 CCGCGG CCGC^GG
SfoI 3320 GGCGCC GGC^GCC
SfuI 3398 TTCGAA TT^CGAA
SgrAI 2769 CGCCGGTG C[GA]^CCGG[CT]G
Tth111I 2469 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 220 CCACTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 999 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 1289 TCCGGA T^CCGGA
1966 TCCGGA
AflIII 623 ACGTGT A^C[GA][CT]GT
894 ACATGT
AhdI 140 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2596 GACAGCCTGTC
AlwNI 2745 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
3205 CAGCACCTG
AocI 477 CCTAAGG CC^T[ACGT]AGG
855 CCTTAGG
AspEI 140 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2596 GACAGCCTGTC
BglI 1329 GCCTGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2770 GCCGGTGGGGC
BlpI 2763 GCTCAGC GC^T[ACGT]AGC
3430 GCTCAGC
Bpu1102I 2763 GCTCAGC GC^T[ACGT]AGC
3430 GCTCAGC
BsaI 850 GAGACC GGTCTC(1/5)
2096 GAGACC
BseAI 1289 TCCGGA T^CCGGA
1966 TCCGGA
BsgI 2533 GTGCAG GTGCAG(16/14)
2722 GTGCAG
BspEI 1289 TCCGGA T^CCGGA
1966 TCCGGA
Bsu36I 477 CCTAAGG CC^T[ACGT]AGG
855 CCTTAGG
CelII 2763 GCTCAGC GC^T[ACGT]AGC
3430 GCTCAGC
DraIII 1720 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
3364 CACAATGTG
DsaI 2618 CCGTGG C^C[GA][CT]GG
3565 CCGCGG
Eam1105I 140 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2596 GACAGCCTGTC
Eco81I 477 CCTAAGG CC^T[ACGT]AGG
855 CCTTAGG
EcoNI 1948 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3210 CCTGCTTCAGG
HincII 1557 GTTAAC GT[CT]^[GA]AC
2937 GTCAAC
HindII 1557 GTTAAC GT[CT]^[GA]AC
2937 GTCAAC
MroI 1289 TCCGGA T^CCGGA
1966 TCCGGA
MslI 2205 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2506 CATCCCAGTG
SapI 2155 GAAGAGC GCTCTTC(1/4)
2404 GAAGAGC
SmlI 336 CTTAAG C^T[CT][GA]AG
1387 CTTGAG
SphI 1715 GCATGC GCATG^C
3011 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
Aor51HI Asp700I AspHI AvaII BamHI
BanI BanII BbvI BclI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EarI Eco47III EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI