Restriction map for
pFN21AA0606
(Linear) (Six-base) MAPSORT of: KIAA0606.seq Check: 1742 from: 1 to: 3615
KIAA0606.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3218 3615
Size: 3218 397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1591 2021 2174 2431 3199 3615
Size: 1591 430 153 257 768 416
Fragments arranged by size:
1591 768 430 416 257 153
AfeI AGC'GCT
Cuts at: 0 1091 2712 3007 3615
Size: 1091 1621 295 608
Fragments arranged by size:
1621 1091 608 295
AflII C'TTAA_G
Cuts at: 0 336 3615
Size: 336 3279
AflIII A'CryG_T
Cuts at: 0 623 894 3615
Size: 623 271 2721
Fragments arranged by size:
2721 623 271
AhdI GACnn_n'nnGTC
Cuts at: 0 145 2601 3615
Size: 145 2456 1014
Fragments arranged by size:
2456 1014 145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 655 1732 3615
Size: 655 1077 1883
Fragments arranged by size:
1883 1077 655
AlwNI CAG_nnn'CTG
Cuts at: 0 2750 3210 3615
Size: 2750 460 405
Fragments arranged by size:
2750 460 405
ApaLI G'TGCA_C
Cuts at: 0 2130 3615
Size: 2130 1485
ApoI r'AATT_y
Cuts at: 0 775 3615
Size: 775 2840
AvrII C'CTAG_G
Cuts at: 0 2574 3615
Size: 2574 1041
BamHI G'GATC_C
Cuts at: 0 2289 2299 3438 3615
Size: 2289 10 1139 177
Fragments arranged by size:
2289 1139 177 10
BanI G'GyrC_C
Cuts at: 0 2112 2251 2517 3219 3320 3615
Size: 2112 139 266 702 101 295
Fragments arranged by size:
2112 702 295 266 139 101
BanII G_rGCy'C
Cuts at: 0 1394 1974 2765 2906 2957 2970 3119 3615
Size: 1394 580 791 141 51 13 149 496
Fragments arranged by size:
1394 791 580 496 149 141 51 13
BbeI G_GCGC'C
Cuts at: 0 3324 3615
Size: 3324 291
BbsI GAAGACnn'nnnn_
Cuts at: 0 3123 3615
Size: 3123 492
BbvCI CC'TCA_GC
Cuts at: 0 3545 3615
Size: 3545 70
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2024 2058 3615
Size: 2024 34 1557
Fragments arranged by size:
2024 1557 34
BclI T'GATC_A
Cuts at: 0 1893 1941 2828 3447 3615
Size: 1893 48 887 619 168
Fragments arranged by size:
1893 887 619 168 48
BfrBI ATG'CAT
Cuts at: 0 210 3615
Size: 210 3405
BglI GCCn_nnn'nGGC
Cuts at: 0 1335 2776 3615
Size: 1335 1441 839
Fragments arranged by size:
1441 1335 839
BglII A'GATC_T
Cuts at: 0 2380 3615
Size: 2380 1235
BlpI GC'TnA_GC
Cuts at: 0 2764 3431 3615
Size: 2764 667 184
Fragments arranged by size:
2764 667 184
Bme1580I G_kGCm'C
Cuts at: 0 1553 2117 2134 2642 3615
Size: 1553 564 17 508 973
Fragments arranged by size:
1553 973 564 508 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 1129 2504 2786 2990 3615
Size: 1129 1375 282 204 625
Fragments arranged by size:
1375 1129 625 282 204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1454 1660 2110 2853 3428 3535 3615
Size: 1454 206 450 743 575 107 80
Fragments arranged by size:
1454 743 575 450 206 107 80
Bpu10I CC'TnA_GC
Cuts at: 0 1798 2971 3545 3615
Size: 1798 1173 574 70
Fragments arranged by size:
1798 1173 574 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1408 3615
Size: 1408 2207
BsaI GGTCTCn'nnnn_
Cuts at: 0 844 2090 3615
Size: 844 1246 1525
Fragments arranged by size:
1525 1246 844
BsaAI yAC'GTr
Cuts at: 0 2532 3615
Size: 2532 1083
BsaHI Gr'CG_yC
Cuts at: 0 141 3128 3321 3615
Size: 141 2987 193 294
Fragments arranged by size:
2987 294 193 141
BsaWI w'CCGG_w
Cuts at: 0 199 1289 1966 2099 3615
Size: 199 1090 677 133 1516
Fragments arranged by size:
1516 1090 677 199 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1977 2007 3615
Size: 1977 30 1608
Fragments arranged by size:
1977 1608 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 270 2626 3127 3493 3615
Size: 270 2356 501 366 122
Fragments arranged by size:
2356 501 366 270 122
BseYI C'CCAG_C
Cuts at: 0 371 1974 2233 2853 3615
Size: 371 1603 259 620 762
Fragments arranged by size:
1603 762 620 371 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2554 2743 3615
Size: 2554 189 872
Fragments arranged by size:
2554 872 189
BsiHKAI G_wGCw'C
Cuts at: 0 1394 2134 2546 2765 2906 3615
Size: 1394 740 412 219 141 709
Fragments arranged by size:
1394 740 709 412 219 141
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1952 3615
Size: 1952 1663
Bsp1286I G_dGCh'C
Cuts at: 0 1394 1553 1974 2117 2134 2546 2642 2765
Size: 1394 159 421 143 17 412 96 123
Cuts at: 2765 2906 2957 2970 3119 3615
Size: 141 51 13 149 496
Fragments arranged by size:
1394 496 421 412 159 149 143 141
123 96 51 17 13
BspEI T'CCGG_A
Cuts at: 0 1289 1966 3615
Size: 1289 677 1649
Fragments arranged by size:
1649 1289 677
BspHI T'CATG_A
Cuts at: 0 1700 2390 3494 3615
Size: 1700 690 1104 121
Fragments arranged by size:
1700 1104 690 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 172 1847 3218 3615
Size: 172 1675 1371 397
Fragments arranged by size:
1675 1371 397 172
BsrDI GCAATG_nn'
Cuts at: 0 639 3615
Size: 639 2976
BsrFI r'CCGG_y
Cuts at: 0 2770 3615
Size: 2770 845
BssSI C'ACGA_G
Cuts at: 0 396 3615
Size: 396 3219
BstAPI GCAn_nnn'nTGC
Cuts at: 0 216 2541 3210 3615
Size: 216 2325 669 405
Fragments arranged by size:
2325 669 405 216
BstBI TT'CG_AA
Cuts at: 0 3399 3615
Size: 3399 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1982 3615
Size: 1982 1633
BstYI r'GATC_y
Cuts at: 0 900 1580 2289 2299 2380 3438 3615
Size: 900 680 709 10 81 1058 177
Fragments arranged by size:
1058 900 709 680 177 81 10
Bsu36I CC'TnA_GG
Cuts at: 0 478 856 3615
Size: 478 378 2759
Fragments arranged by size:
2759 478 378
BtgI C'CryG_G
Cuts at: 0 2618 3565 3615
Size: 2618 947 50
Fragments arranged by size:
2618 947 50
BtsI GCAGTG_nn'
Cuts at: 0 2904 3171 3615
Size: 2904 267 444
Fragments arranged by size:
2904 444 267
DraI TTT'AAA
Cuts at: 0 464 3615
Size: 464 3151
DraIII CAC_nnn'GTG
Cuts at: 0 1725 3369 3615
Size: 1725 1644 246
Fragments arranged by size:
1725 1644 246
EaeI y'GGCC_r
Cuts at: 0 1236 3615
Size: 1236 2379
EarI CTCTTCn'nnn_
Cuts at: 0 436 538 1107 1481 2150 2399 2407 3401
Size: 436 102 569 374 669 249 8 994
Cuts at: 3401 3470 3615
Size: 69 145
Fragments arranged by size:
994 669 569 436 374 249 145 102
69 8
EcoICRI GAG'CTC
Cuts at: 0 1392 2763 2904 3615
Size: 1392 1371 141 711
Fragments arranged by size:
1392 1371 711 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1454 1591 1660 2021 2110 2174 2431 2853
Size: 1454 137 69 361 89 64 257 422
Cuts at: 2853 3199 3428 3535 3615
Size: 346 229 107 80
Fragments arranged by size:
1454 422 361 346 257 229 137 107
89 80 69 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1952 3214 3615
Size: 1952 1262 401
Fragments arranged by size:
1952 1262 401
EcoO109I rG'GnC_Cy
Cuts at: 0 161 3615
Size: 161 3454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 317 3312 3615
Size: 317 2995 303
Fragments arranged by size:
2995 317 303
FspI TGC'GCA
Cuts at: 0 2632 3615
Size: 2632 983
HaeII r_GCGC'y
Cuts at: 0 1093 2714 2771 3009 3051 3065 3080 3260
Size: 1093 1621 57 238 42 14 15 180
Cuts at: 3260 3324 3615
Size: 64 291
Fragments arranged by size:
1621 1093 291 238 180 64 57 42
15 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 131 163 2372 2404 2587 2619 2820 2827
Size: 131 32 2209 32 183 32 201 7
Cuts at: 2827 2852 2859 2920 2952 3615
Size: 25 7 61 32 663
Fragments arranged by size:
2209 663 201 183 131 61 32 32
32 32 25 7 7
HincII GTy'rAC
Cuts at: 0 1559 2939 3615
Size: 1559 1380 676
Fragments arranged by size:
1559 1380 676
HindIII A'AGCT_T
Cuts at: 0 988 2003 2242 3615
Size: 988 1015 239 1373
Fragments arranged by size:
1373 1015 988 239
HpaI GTT'AAC
Cuts at: 0 1559 3615
Size: 1559 2056
KasI G'GCGC_C
Cuts at: 0 3320 3615
Size: 3320 295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 642 674 710 1763 3059 3110 3615
Size: 628 14 32 36 1053 1296 51 505
Fragments arranged by size:
1296 1053 628 505 51 36 32 14
MscI TGG'CCA
Cuts at: 0 1238 3615
Size: 1238 2377
MslI CAynn'nnrTG
Cuts at: 0 2209 2510 3615
Size: 2209 301 1105
Fragments arranged by size:
2209 1105 301
MspA1I CmG'CkG
Cuts at: 0 502 1928 1978 2257 2367 2876 3561 3567
Size: 502 1426 50 279 110 509 685 6
Cuts at: 3567 3579 3588 3615
Size: 12 9 27
Fragments arranged by size:
1426 685 509 502 279 110 50 27
12 9 6
NarI GG'CG_CC
Cuts at: 0 3321 3615
Size: 3321 294
NsiI A_TGCA'T
Cuts at: 0 212 3615
Size: 212 3403
NspI r_CATG'y
Cuts at: 0 214 898 961 1719 3015 3615
Size: 214 684 63 758 1296 600
Fragments arranged by size:
1296 758 684 600 214 63
PciI A'CATG_T
Cuts at: 0 894 3615
Size: 894 2721
PflMI CCAn_nnn'nTGG
Cuts at: 0 226 3615
Size: 226 3389
PfoI T'CCnGG_A
Cuts at: 0 2292 3615
Size: 2292 1323
PmlI CAC'GTG
Cuts at: 0 2532 3615
Size: 2532 1083
PpuMI rG'GwC_Cy
Cuts at: 0 161 3615
Size: 161 3454
PstI C_TGCA'G
Cuts at: 0 598 1856 3178 3615
Size: 598 1258 1322 437
Fragments arranged by size:
1322 1258 598 437
PvuII CAG'CTG
Cuts at: 0 502 1978 3561 3579 3588 3615
Size: 502 1476 1583 18 9 27
Fragments arranged by size:
1583 1476 502 27 18 9
SacI G_AGCT'C
Cuts at: 0 1394 2765 2906 3615
Size: 1394 1371 141 709
Fragments arranged by size:
1394 1371 709 141
SacII CC_GC'GG
Cuts at: 0 3568 3615
Size: 3568 47
SapI GCTCTTCn'nnn_
Cuts at: 0 2150 2399 3615
Size: 2150 249 1216
Fragments arranged by size:
2150 1216 249
SfcI C'TryA_G
Cuts at: 0 435 594 862 952 1852 1952 3174 3383
Size: 435 159 268 90 900 100 1222 209
Cuts at: 3383 3615
Size: 232
Fragments arranged by size:
1222 900 435 268 232 209 159 100
90
SfoI GGC'GCC
Cuts at: 0 3322 3615
Size: 3322 293
SgrAI Cr'CCGG_yG
Cuts at: 0 2770 3615
Size: 2770 845
SmlI C'TyrA_G
Cuts at: 0 336 1387 3615
Size: 336 1051 2228
Fragments arranged by size:
2228 1051 336
SphI G_CATG'C
Cuts at: 0 1719 3015 3615
Size: 1719 1296 600
Fragments arranged by size:
1719 1296 600
StyI C'CwwG_G
Cuts at: 0 359 2574 3185 3615
Size: 359 2215 611 430
Fragments arranged by size:
2215 611 430 359
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2805 3615
Size: 2805 810
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 830 862 2521 2553 3615
Size: 382 32 416 32 1659 32 1062
Fragments arranged by size:
1659 1062 416 382 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2472 3615
Size: 2472 1143
XbaI T'CTAG_A
Cuts at: 0 999 3615
Size: 999 2616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 128 2484 2877 3615
Size: 128 2356 393 738
Fragments arranged by size:
2356 738 393 128
XmnI GAAnn'nnTTC
Cuts at: 0 71 1115 1463 2053 3615
Size: 71 1044 348 590 1562
Fragments arranged by size:
1562 1044 590 348 71
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AhdI AlfI AlwNI
ApaLI ApoI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtsI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI MmeI MscI MslI MspA1I
NarI NsiI NspI PciI PflMI PfoI PmlI PpuMI
PstI PvuII SacI SacII SapI SfcI SfoI SgrAI
SmlI SphI StyI TaqII TstI Tth111I XbaI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AleI AloI ApaI
AscI AseI AsiSI AvaI BaeI BciVI BmgBI BmtI
BplI BsaBI BsiEI BsiWI BsmI BsrBI BsrGI BssHII
BstEII BstZ17I BtgZI ClaI CspCI DrdI EagI EciI
EcoRI EcoRV FseI FspAI KpnI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI
XhoI XmaI ZraI