Restriction map for
pFN21AA0599
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# NO CUT ENZYMES ( 69 enzymes) #
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AclI AcsI AflIII Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspI AvrII BamHI BciVI
BclI BlnI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DraI DrdI Eco47III EcoO65I
EcoRI FbaI HindIII HpaI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I PvuI
RcaI SalI SbfI SexAI SfuI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 3508 GACGTC GACGT^C
Acc65I 1256 GGTACC G^GTACC
AflII 2077 CTTAAG C^TTAAG
AhdI 2230 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 3170 GGATC GGATC(4/5)
AocI 1894 CCTGAGG CC^T[ACGT]AGG
Asp718I 1256 GGTACC G^GTACC
AspEI 2230 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1331 GGCGCC GGCGC^C
BbrPI 3112 CACGTG CAC^GTG
BcgI 1847 GCATGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2077 CTTAAG C^TTAAG
BglII 2810 AGATCT A^GATCT
BsaBI 1044 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1951 GAGACG CGTCTC(1/5)
BsmI 586 GAATGC GAATGC(1/-1)
BstYI 2810 AGATCT [GA]^GATC[CT]
Bsu36I 1894 CCTGAGG CC^T[ACGT]AGG
CpoI 836 CGGTCCG CG^G[AT]CCG
DraIII 3601 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2230 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1894 CCTGAGG CC^T[ACGT]AGG
EcoRV 532 GATATC GAT^ATC
EcoT22I 588 ATGCAT ATGCA^T
HincII 3589 GTCAAC GT[CT]^[GA]AC
HindII 3589 GTCAAC GT[CT]^[GA]AC
KasI 1331 GGCGCC G^GCGCC
KpnI 1256 GGTACC GGTAC^C
MamI 1044 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2810 AGATCT [GA]^GATC[CT]
NarI 1331 GGCGCC GG^CGCC
NcoI 104 CCATGG C^CATGG
NsiI 588 ATGCAT ATGCA^T
PmaCI 3112 CACGTG CAC^GTG
PmlI 3112 CACGTG CAC^GTG
RsrII 836 CGGTCCG CG^G[AT]CCG
ScaI 1834 AGTACT AGT^ACT
SfiI 2994 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1331 GGCGCC GGC^GCC
SgrAI 3869 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 322 TACGTA TAC^GTA
XhoII 2810 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccIII 2833 TCCGGA T^CCGGA
3167 TCCGGA
AgeI 2456 ACCGGT A^CCGGT
3870 ACCGGT
AviII 335 TGCGCA TGC^GCA
941 TGCGCA
BbsI 2460 GTCTTC GAAGAC(2/6)
2829 GTCTTC
BbvCI 2343 CCTCAGC CCTCAGC(-5/-2)
3477 CCTCAGC
BfaI 1180 CTAG C^TAG
2512 CTAG
BpuAI 2460 GTCTTC GAAGAC(2/6)
2829 GTCTTC
BseAI 2833 TCCGGA T^CCGGA
3167 TCCGGA
BspEI 2833 TCCGGA T^CCGGA
3167 TCCGGA
BsrBI 40 GAGCGG CCGCTC(-3/-3)
1273 GAGCGG
BsrDI 2175 CATTGC GCAATG(2/0)
944 GCAATG
EcoNI 18 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1872 CCTGCCAGAGG
FseI 3454 GGCCGGCC GGCCGG^CC
3568 GGCCGGCC
FspI 335 TGCGCA TGC^GCA
941 TGCGCA
KspI 2257 CCGCGG CCGC^GG
3576 CCGCGG
MaeI 1180 CTAG C^TAG
2512 CTAG
MroI 2833 TCCGGA T^CCGGA
3167 TCCGGA
MseI 2078 TTAA T^TAA
2522 TTAA
PinAI 2456 ACCGGT A^CCGGT
3870 ACCGGT
SacI 2725 GAGCTC GAGCT^C
3517 GAGCTC
SacII 2257 CCGCGG CCGC^GG
3576 CCGCGG
SapI 1546 GAAGAGC GCTCTTC(1/4)
3243 GAAGAGC
SmlI 694 CTCAAG C^T[CT][GA]AG
2077 CTTAAG
SphI 590 GCATGC GCATG^C
1404 GCATGC
StuI 1553 AGGCCT AGG^CCT
4001 AGGCCT
TaqI 1052 TCGA T^CGA
1856 TCGA
Tru9I 2078 TTAA T^TAA
2522 TTAA
TthHB8I 1052 TCGA T^CGA
1856 TCGA
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# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TfiI TseI Tsp45I Tsp509I TspRI
Van91I XcmI XmaCI XmaI