Restriction map for
pFN21AA0598
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Alw44I ApaLI AscI Asp718I
AspI BalI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BglII
BsaBI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssSI
Bst1107I BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI EarI EclXI
Eco52I EcoRI EcoRV FbaI FseI
HindIII HpaI KasI KpnI Ksp632I
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 1315 GTCTAC GT^[AC][GT]AC
AhdI 1157 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 969 GATCC GGATC(4/5)
AlwNI 1480 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 829 CCTGAGG CC^T[ACGT]AGG
Asp700I 735 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1157 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 655 GTGCTC G[AT]GC[AT]^C
AviII 695 TGCGCA TGC^GCA
AvrII 1491 CCTAGG C^CTAGG
BbrPI 105 CACGTG CAC^GTG
BcnI 872 CCCGG CC^[GC]GG
BlnI 1491 CCTAGG C^CTAGG
BlpI 1392 GCTCAGC GC^T[ACGT]AGC
BpmI 1354 CTCCAG CTGGAG(16/14)
Bpu1102I 1392 GCTCAGC GC^T[ACGT]AGC
BsaI 914 GAGACC GGTCTC(1/5)
BsaWI 1565 ACCGGA [AT]^CCGG[AT]
BseRI 604 GAGGAG GAGGAG(10/8)
BsgI 823 CTGCAC GTGCAG(16/14)
BsiHKAI 655 GTGCTC G[AT]GC[AT]^C
BsmAI 914 GAGAC GTCTC(1/5)
BsrBI 675 CCGCTC CCGCTC(-3/-3)
BssHII 1305 GCGCGC G^CGCGC
BstBI 1221 TTCGAA TT^CGAA
BstEII 88 GGTCACC G^GT[ACGT]ACC
BstPI 88 GGTCACC G^GT[ACGT]ACC
BstYI 968 AGATCC [GA]^GATC[CT]
Bsu36I 829 CCTGAGG CC^T[ACGT]AGG
CelII 1392 GCTCAGC GC^T[ACGT]AGC
DraIII 1050 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 956 TGGCCG [CT]^GGCC[GA]
Eam1105I 1157 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 829 CCTGAGG CC^T[ACGT]AGG
EcoNI 693 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 88 GGTCACC G^GT[ACGT]ACC
EcoT22I 1442 ATGCAT ATGCA^T
FspI 695 TGCGCA TGC^GCA
HincII 61 GTCAAC GT[CT]^[GA]AC
HindII 61 GTCAAC GT[CT]^[GA]AC
KspI 1232 CCGCGG CCGC^GG
MflI 968 AGATCC [GA]^GATC[CT]
NciI 872 CCCGG CC^[GC]GG
NdeI 544 CATATG CA^TATG
NsiI 1442 ATGCAT ATGCA^T
NspI 1286 GCATGC [GA]CATG^[CT]
NspV 1221 TTCGAA TT^CGAA
PflMI 1049 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 105 CACGTG CAC^GTG
PmlI 105 CACGTG CAC^GTG
SacII 1232 CCGCGG CCGC^GG
SfiI 1577 GGCCCATGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1221 TTCGAA TT^CGAA
SphI 1286 GCATGC GCATG^C
SspI 424 AATATT AAT^ATT
StuI 701 AGGCCT AGG^CCT
Van91I 1049 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 968 AGATCC [GA]^GATC[CT]
XmnI 735 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 125 AAATTC [GA]^AATT[CT]
568 AAATTC
AcyI 688 GACGCC G[GA]^CG[CT]C
1553 GGCGTC
AflII 526 CTTAAG C^TTAAG
1500 CTTAAG
Aor51HI 668 AGCGCT AGC^GCT
878 AGCGCT
ApoI 125 AAATTC [GA]^AATT[CT]
568 AAATTC
AseI 13 ATTAAT AT^TAAT
459 ATTAAT
AsnI 13 ATTAAT AT^TAAT
459 ATTAAT
AvaI 612 CTCGGG C^[CT]CG[GA]G
1558 CCCGAG
BfrI 526 CTTAAG C^TTAAG
1500 CTTAAG
BsaAI 105 CACGTG [CT]AC^GT[GA]
652 TACGTG
BsaHI 688 GACGCC G[GA]^CG[CT]C
1553 GGCGTC
BsoBI 612 CTCGGG C^[CT]CG[GA]G
1558 CCCGAG
BspMI 57 GCAGGT ACCTGC(4/8)
1644 GCAGGT
BstXI 359 CCAGCTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
859 CCACACTGGTGG
DsaI 926 CCGTGG C^C[GA][CT]GG
1232 CCGCGG
Eco47III 668 AGCGCT AGC^GCT
878 AGCGCT
HaeII 668 AGCGCT [GA]GCGC^[CT]
878 AGCGCT
HapII 873 CCGG C^CGG
1566 CCGG
Hin1I 688 GACGCC G[GA]^CG[CT]C
1553 GGCGTC
HinfI 887 GAATC G^A[ACGT]TC
1602 GATTC
HpaII 873 CCGG C^CGG
1566 CCGG
MslI 860 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1341 CATGCCTGTG
MspI 873 CCGG C^CGG
1566 CCGG
PpuMI 1175 GGGACCC [GA]G^G[AT]CC[CT]
1646 AGGTCCT
PshBI 13 ATTAAT AT^TAAT
459 ATTAAT
PstI 982 CTGCAG CTGCA^G
1267 CTGCAG
PvuII 28 CAGCTG CAG^CTG
1270 CAGCTG
SmlI 526 CTTAAG C^T[CT][GA]AG
1500 CTTAAG
TfiI 887 GAATC G^A[AT]TC
1602 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AvaII BanII BbsI BbvI BfaI
BglI BmyI BpuAI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HgaI HhaI HinP1I
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I