(Circular) (Six-base) MAPSORT of: pFN21AA0598.seq Check: 1348 from: 1 to: 6388 pFN21AA0598 6388 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4198 278 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3321 3731 1937 Size: 1384 410 4594 Fragments arranged by size: 4594 1384 410 Acc65I G'GTAC_C Cuts at: 3709 3709 Size: 6388 AclI AA'CG_TT Cuts at: 1563 4515 4888 1563 Size: 2952 373 3063 Fragments arranged by size: 3063 2952 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2211 4451 5835 6103 1632 Size: 579 2240 1384 268 1917 Fragments arranged by size: 2240 1917 1384 579 268 AfeI AGC'GCT Cuts at: 2675 2885 2675 Size: 210 6178 AflII C'TTAA_G Cuts at: 820 1017 2531 3505 820 Size: 197 1514 974 3703 Fragments arranged by size: 3703 1514 974 197 AflIII A'CryG_T Cuts at: 1203 5287 6264 1203 Size: 4084 977 1327 Fragments arranged by size: 4084 1327 977 AgeI A'CCGG_T Cuts at: 5219 5219 Size: 6388 AhdI GACnn_n'nnGTC Cuts at: 3167 5117 3167 Size: 1950 4438 AleI CACnn'nnGTG Cuts at: 1166 2869 1166 Size: 1703 4685 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6388 AlwNI CAG_nnn'CTG Cuts at: 3490 4131 5703 3490 Size: 641 1572 4175 Fragments arranged by size: 4175 1572 641 ApaI G_GGCC'C Cuts at: 2084 2308 3504 3585 2084 Size: 224 1196 81 4887 Fragments arranged by size: 4887 1196 224 81 ApaLI G'TGCA_C Cuts at: 4445 5601 4445 Size: 1156 5232 ApoI r'AATT_y Cuts at: 1493 2130 2573 3697 3923 4075 6184 1493 Size: 637 443 1124 226 152 2109 1697 Fragments arranged by size: 2109 1697 1124 637 443 226 152 AseI AT'TA_AT Cuts at: 160 1032 2019 2465 4942 160 Size: 872 987 446 2477 1606 Fragments arranged by size: 2477 1606 987 872 446 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 AvaI C'yCGr_G Cuts at: 1870 1943 2617 3563 3713 1870 Size: 73 674 946 150 4545 Fragments arranged by size: 4545 946 674 150 73 AvrII C'CTAG_G Cuts at: 3496 3496 Size: 6388 BamHI G'GATC_C Cuts at: 3718 4085 3718 Size: 367 6021 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3709 5164 618 Size: 325 217 627 1922 1455 1842 Fragments arranged by size: 1922 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2084 2308 2597 3372 3504 3542 3585 3707 Size: 693 224 289 775 132 38 43 122 Cuts at: 3707 1391 Size: 4072 Fragments arranged by size: 4072 775 693 289 224 132 122 43 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2532 3472 3686 928 Size: 971 633 940 214 3630 Fragments arranged by size: 3630 971 940 633 214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4579 4613 1903 Size: 34 2642 34 3678 Fragments arranged by size: 3678 2642 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4280 5490 4280 Size: 1210 5178 BclI T'GATC_A Cuts at: 1548 1548 Size: 6388 BfrBI ATG'CAT Cuts at: 3449 3449 Size: 6388 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2079 3355 3589 Size: 107 122 71 631 605 407 1276 234 Cuts at: 3589 4999 136 Size: 1410 1525 Fragments arranged by size: 1525 1410 1276 631 605 407 234 122 107 71 BglII A'GATC_T Cuts at: 6383 6383 Size: 6388 BlpI GC'TnA_GC Cuts at: 3398 3805 3398 Size: 407 5981 Bme1580I G_kGCm'C Cuts at: 1790 2084 2108 2308 3504 3585 4449 5605 1790 Size: 294 24 200 1196 81 864 1156 2573 Fragments arranged by size: 2573 1196 1156 864 294 200 81 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6388 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2215 4119 5077 Size: 416 53 83 399 24 798 1904 958 Cuts at: 5077 442 Size: 1753 Fragments arranged by size: 1904 1753 958 798 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6388 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2632 2632 Size: 6388 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3344 5048 1239 Size: 2105 1704 2579 Fragments arranged by size: 2579 2105 1704 Bpu10I CC'TnA_GC Cuts at: 3176 3176 Size: 6388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4513 5378 5676 4513 Size: 865 298 5225 Fragments arranged by size: 5225 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2913 5051 882 Size: 617 1414 2138 2219 Fragments arranged by size: 2219 2138 1414 617 BsaAI yAC'GTr Cuts at: 493 1204 2112 2659 4179 493 Size: 711 908 547 1520 2702 Fragments arranged by size: 2702 1520 908 711 547 BsaBI GATnn'nnATC Cuts at: 6382 6382 Size: 6388 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2694 3559 4195 4577 Size: 53 83 186 936 1161 865 636 382 Cuts at: 4577 5212 275 Size: 635 1451 Fragments arranged by size: 1451 1161 936 865 636 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3570 4821 5219 5493 5640 6051 3570 Size: 1251 398 274 147 411 3907 Fragments arranged by size: 3907 1251 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3182 3212 1178 Size: 30 165 30 1779 30 4354 Fragments arranged by size: 4354 1779 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2624 1186 Size: 1438 4950 BseYI C'CCAG_C Cuts at: 2354 2363 5591 6087 2354 Size: 9 3228 496 2655 Fragments arranged by size: 3228 2655 496 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2813 1163 Size: 573 1077 4738 Fragments arranged by size: 4738 1077 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3838 4599 5627 1540 Size: 275 186 1837 761 1028 2301 Fragments arranged by size: 2301 1837 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2664 4449 4534 5605 2664 Size: 1785 85 1071 3447 Fragments arranged by size: 3447 1785 1071 85 BsmI GAATG_Cn' Cuts at: 3899 3992 3899 Size: 93 6295 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2108 2308 2597 2664 3372 3504 Size: 399 294 24 200 289 67 708 132 Cuts at: 3504 3542 3585 3707 4449 4534 5605 1391 Size: 38 43 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 708 399 294 289 200 132 122 85 67 43 38 24 BspEI T'CCGG_A Cuts at: 6051 6051 Size: 6388 BspHI T'CATG_A Cuts at: 4277 6007 4277 Size: 1730 4658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 3640 3743 844 Size: 1209 1587 103 3489 Fragments arranged by size: 3489 1587 1209 103 BsrBI CCG'CTC Cuts at: 1394 1823 2682 4275 1394 Size: 429 859 1593 3507 Fragments arranged by size: 3507 1593 859 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4883 5057 65 Size: 1183 3635 174 1396 Fragments arranged by size: 3635 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5032 5219 1700 Size: 110 3222 187 2869 Fragments arranged by size: 3222 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6388 BssHII G'CGCG_C Cuts at: 1925 3310 1925 Size: 1385 5003 BssSI C'ACGA_G Cuts at: 4448 5460 4448 Size: 1012 5376 BstAPI GCAn_nnn'nTGC Cuts at: 794 2022 2545 3551 794 Size: 1228 523 1006 3631 Fragments arranged by size: 3631 1228 1006 523 BstBI TT'CG_AA Cuts at: 3227 5192 3227 Size: 1965 4423 BstEII G'GTnAC_C Cuts at: 1184 2093 1184 Size: 909 5479 BstXI CCAn_nnnn'nTGG Cuts at: 2371 2871 2371 Size: 500 5888 BstYI r'GATC_y Cuts at: 1972 2973 3718 4085 4470 4487 5939 6025 6037 Size: 1001 745 367 385 17 1452 86 12 Cuts at: 6037 6383 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1001 745 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2835 2835 Size: 6388 BtgI C'CryG_G Cuts at: 513 1065 2931 3237 513 Size: 552 1866 306 3664 Fragments arranged by size: 3664 1866 552 306 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2254 487 Size: 683 56 1028 4621 Fragments arranged by size: 4621 1028 683 56 BtsI GCAGTG_nn' Cuts at: 2011 3269 3305 3904 4698 4718 2011 Size: 1258 36 599 794 20 3681 Fragments arranged by size: 3681 1258 794 599 36 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6388 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6353 DraI TTT'AAA Cuts at: 3692 3692 Size: 6388 DraIII CAC_nnn'GTG Cuts at: 3060 3060 Size: 6388 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5395 809 Size: 507 4079 1802 Fragments arranged by size: 4079 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2961 3835 4724 Size: 54 1425 50 275 18 1131 874 889 Cuts at: 4724 6353 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1131 889 874 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3835 1537 Size: 275 2023 4090 Fragments arranged by size: 4090 2023 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4318 1353 Size: 2965 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2738 2839 2888 4975 5349 5495 Size: 1064 138 1149 101 49 2087 374 146 Cuts at: 5495 387 Size: 1280 Fragments arranged by size: 2087 1280 1149 1064 374 146 138 101 49 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 3344 4451 5048 5835 6103 1239 Size: 393 579 1133 1107 597 787 268 1524 Fragments arranged by size: 1524 1133 1107 787 597 579 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2702 2702 Size: 6388 EcoO109I rG'GnC_Cy Cuts at: 2080 2081 2304 3181 3500 3501 3581 3627 3652 Size: 1 223 877 319 1 80 46 25 Cuts at: 3652 2080 Size: 4816 Fragments arranged by size: 4816 877 319 223 80 46 25 1 1 EcoRI G'AATT_C Cuts at: 3697 4075 3697 Size: 378 6010 FspI TGC'GCA Cuts at: 2702 4894 2702 Size: 2192 4196 HaeII r_GCGC'y Cuts at: 1678 2677 2887 5535 1678 Size: 999 210 2648 2531 Fragments arranged by size: 2648 2531 999 210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2232 2264 3153 3185 3434 Size: 32 135 32 660 32 889 32 249 Cuts at: 3434 3466 5029 5061 5103 5135 1373 Size: 32 1563 32 42 32 2626 Fragments arranged by size: 2626 1563 889 660 249 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2068 3732 3978 669 Size: 988 281 130 1664 246 3079 Fragments arranged by size: 3079 1664 988 281 246 130 HindIII A'AGCT_T Cuts at: 748 748 Size: 6388 HpaI GTT'AAC Cuts at: 3978 3978 Size: 6388 KpnI G_GTAC'C Cuts at: 3713 3713 Size: 6388 MfeI C'AATT_G Cuts at: 3987 3987 Size: 6388 MluI A'CGCG_T Cuts at: 6264 6264 Size: 6388 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3477 5502 5686 1305 Size: 2172 2025 184 2007 Fragments arranged by size: 2172 2025 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4566 Fragments arranged by size: 4566 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2869 3350 4346 4705 4864 518 Size: 648 1703 481 996 359 159 2042 Fragments arranged by size: 2042 1703 996 648 481 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 3239 3277 3804 4481 5629 5874 1606 Size: 429 1204 38 527 677 1148 245 2120 Fragments arranged by size: 2120 1204 1148 677 527 429 245 38 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5836 NdeI CA'TA_TG Cuts at: 387 2550 387 Size: 2163 4225 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6388 NotI GC'GGCC_GC Cuts at: 3835 3835 Size: 6388 NsiI A_TGCA'T Cuts at: 3451 3451 Size: 6388 NspI r_CATG'y Cuts at: 1132 1229 3295 3746 5291 1132 Size: 97 2066 451 1545 2229 Fragments arranged by size: 2229 2066 1545 451 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6388 PciI A'CATG_T Cuts at: 5287 5287 Size: 6388 PflMI CCAn_nnn'nTGG Cuts at: 1108 3060 1108 Size: 1952 4436 PmeI GTTT'AAAC Cuts at: 3692 3692 Size: 6388 PmlI CAC'GTG Cuts at: 2112 2112 Size: 6388 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2828 2860 4457 4489 5970 6002 2828 Size: 32 1597 32 1481 32 3214 Fragments arranged by size: 3214 1597 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3181 3652 3181 Size: 471 5917 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6388 PsiI TTA'TAA Cuts at: 3958 3958 Size: 6388 PspOMI G'GGCC_C Cuts at: 2080 2304 3500 3581 2080 Size: 224 1196 81 4887 Fragments arranged by size: 4887 1196 224 81 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2597 2629 2597 Size: 32 6356 PstI C_TGCA'G Cuts at: 830 2991 3276 3740 830 Size: 2161 285 464 3478 Fragments arranged by size: 3478 2161 464 285 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6388 PvuII CAG'CTG Cuts at: 2035 3277 2035 Size: 1242 5146 SacII CC_GC'GG Cuts at: 3240 3240 Size: 6388 SalI G'TCGA_C Cuts at: 1936 3730 1936 Size: 1794 4594 SanDI GG'GwC_CC Cuts at: 3181 3181 Size: 6388 SbfI CC_TGCA'GG Cuts at: 3740 3740 Size: 6388 ScaI AGT'ACT Cuts at: 4636 6348 4636 Size: 1712 4676 SfcI C'TryA_G Cuts at: 826 1045 2987 3272 3622 3736 4871 5552 5743 Size: 219 1942 285 350 114 1135 681 191 Cuts at: 5743 826 Size: 1471 Fragments arranged by size: 1942 1471 1135 681 350 285 219 191 114 SfiI GGCCn_nnn'nGGCC Cuts at: 3589 3589 Size: 6388 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6388 SmaI CCC'GGG Cuts at: 1872 3715 1872 Size: 1843 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2531 3505 4492 5393 5655 820 Size: 197 926 588 974 987 901 262 1553 Fragments arranged by size: 1553 987 974 926 901 588 262 197 SnaBI TAC'GTA Cuts at: 493 4179 493 Size: 3686 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6388 SphI G_CATG'C Cuts at: 1132 3295 3746 1132 Size: 2163 451 3774 Fragments arranged by size: 3774 2163 451 SspI AAT'ATT Cuts at: 5 52 2431 4312 5 Size: 47 2379 1881 2081 Fragments arranged by size: 2379 2081 1881 47 StuI AGG'CCT Cuts at: 1517 2708 1517 Size: 1191 5197 StyI C'CwwG_G Cuts at: 513 1065 2074 2274 3496 3827 513 Size: 552 1009 200 1222 331 3074 Fragments arranged by size: 3074 1222 1009 552 331 200 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4415 4432 4585 4770 5215 325 Size: 1255 2835 17 153 185 445 1498 Fragments arranged by size: 2835 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3198 3462 4634 Size: 274 80 33 51 1443 1221 264 1172 Cuts at: 4634 6346 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1221 1172 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2399 2431 2958 2990 6348 6380 2399 Size: 32 527 32 3358 32 2407 Fragments arranged by size: 3358 2407 527 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6388 XbaI T'CTAG_A Cuts at: 3724 3724 Size: 6388 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6388 XmaI C'CCGG_G Cuts at: 1870 3713 1870 Size: 1843 4545 XmnI GAAnn'nnTTC Cuts at: 2744 4517 2744 Size: 1773 4615 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4196 276 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BbvCI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspXI RsrII SacI SapI SexAI SfoI SrfI SwaI XcmI