Restriction map for
pFN21AA0598
(Linear) (Six-base) MAPSORT of: KIAA0598.seq Check: 431 from: 1 to: 1683
KIAA0598.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 1316 1683
Size: 1316 367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 1683
Size: 206 1477
AfeI AGC'GCT
Cuts at: 0 670 880 1683
Size: 670 210 803
Fragments arranged by size:
803 670 210
AflII C'TTAA_G
Cuts at: 0 526 1500 1683
Size: 526 974 183
Fragments arranged by size:
974 526 183
AhdI GACnn_n'nnGTC
Cuts at: 0 1162 1683
Size: 1162 521
AleI CACnn'nnGTG
Cuts at: 0 864 1683
Size: 864 819
AlwNI CAG_nnn'CTG
Cuts at: 0 1485 1683
Size: 1485 198
ApaI G_GGCC'C
Cuts at: 0 79 303 1499 1580 1683
Size: 79 224 1196 81 103
Fragments arranged by size:
1196 224 103 81 79
ApoI r'AATT_y
Cuts at: 0 125 568 1683
Size: 125 443 1115
Fragments arranged by size:
1115 443 125
AseI AT'TA_AT
Cuts at: 0 14 460 1683
Size: 14 446 1223
Fragments arranged by size:
1223 446 14
AvaI C'yCGr_G
Cuts at: 0 612 1558 1683
Size: 612 946 125
Fragments arranged by size:
946 612 125
AvrII C'CTAG_G
Cuts at: 0 1491 1683
Size: 1491 192
BanII G_rGCy'C
Cuts at: 0 79 303 592 1367 1499 1537 1580 1683
Size: 79 224 289 775 132 38 43 103
Fragments arranged by size:
775 289 224 132 103 79 43 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 527 1467 1681 1683
Size: 527 940 214 2
Fragments arranged by size:
940 527 214 2
BfrBI ATG'CAT
Cuts at: 0 1444 1683
Size: 1444 239
BglI GCCn_nnn'nGGC
Cuts at: 0 74 1350 1584 1683
Size: 74 1276 234 99
Fragments arranged by size:
1276 234 99 74
BlpI GC'TnA_GC
Cuts at: 0 1393 1683
Size: 1393 290
Bme1580I G_kGCm'C
Cuts at: 0 79 103 303 1499 1580 1683
Size: 79 24 200 1196 81 103
Fragments arranged by size:
1196 200 103 81 79 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 210 1683
Size: 210 1473
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 627 1683
Size: 627 1056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 1683
Size: 1339 344
Bpu10I CC'TnA_GC
Cuts at: 0 1171 1683
Size: 1171 512
BsaI GGTCTCn'nnnn_
Cuts at: 0 908 1683
Size: 908 775
BsaAI yAC'GTr
Cuts at: 0 107 654 1683
Size: 107 547 1029
Fragments arranged by size:
1029 547 107
BsaHI Gr'CG_yC
Cuts at: 0 689 1554 1683
Size: 689 865 129
Fragments arranged by size:
865 689 129
BsaWI w'CCGG_w
Cuts at: 0 1565 1683
Size: 1565 118
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1177 1207 1683
Size: 1177 30 476
Fragments arranged by size:
1177 476 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 1683
Size: 619 1064
BseYI C'CCAG_C
Cuts at: 0 349 358 1683
Size: 349 9 1325
Fragments arranged by size:
1325 349 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1683
Size: 808 875
BsiHKAI G_wGCw'C
Cuts at: 0 659 1683
Size: 659 1024
Bsp1286I G_dGCh'C
Cuts at: 0 79 103 303 592 659 1367 1499 1537
Size: 79 24 200 289 67 708 132 38
Cuts at: 1537 1580 1683
Size: 43 103
Fragments arranged by size:
708 289 200 132 103 79 67 43
38 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 1635 1683
Size: 48 1587 48
Fragments arranged by size:
1587 48 48
BsrBI CCG'CTC
Cuts at: 0 677 1683
Size: 677 1006
BssHII G'CGCG_C
Cuts at: 0 1305 1683
Size: 1305 378
BstAPI GCAn_nnn'nTGC
Cuts at: 0 17 540 1546 1683
Size: 17 523 1006 137
Fragments arranged by size:
1006 523 137 17
BstBI TT'CG_AA
Cuts at: 0 1222 1683
Size: 1222 461
BstEII G'GTnAC_C
Cuts at: 0 88 1683
Size: 88 1595
BstXI CCAn_nnnn'nTGG
Cuts at: 0 366 866 1683
Size: 366 500 817
Fragments arranged by size:
817 500 366
BstYI r'GATC_y
Cuts at: 0 968 1683
Size: 968 715
Bsu36I CC'TnA_GG
Cuts at: 0 830 1683
Size: 830 853
BtgI C'CryG_G
Cuts at: 0 926 1232 1683
Size: 926 306 451
Fragments arranged by size:
926 451 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 249 1683
Size: 249 1434
BtsI GCAGTG_nn'
Cuts at: 0 6 1264 1300 1683
Size: 6 1258 36 383
Fragments arranged by size:
1258 383 36 6
DraIII CAC_nnn'GTG
Cuts at: 0 1055 1683
Size: 1055 628
EaeI y'GGCC_r
Cuts at: 0 956 1683
Size: 956 727
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 733 834 883 1683
Size: 733 101 49 800
Fragments arranged by size:
800 733 101 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 1339 1683
Size: 206 1133 344
Fragments arranged by size:
1133 344 206
EcoNI CCTnn'n_nnAGG
Cuts at: 0 697 1683
Size: 697 986
EcoO109I rG'GnC_Cy
Cuts at: 0 75 76 299 1176 1495 1496 1576 1622
Size: 75 1 223 877 319 1 80 46
Cuts at: 1622 1647 1683
Size: 25 36
Fragments arranged by size:
877 319 223 80 75 46 36 25
1 1
FspI TGC'GCA
Cuts at: 0 697 1683
Size: 697 986
HaeII r_GCGC'y
Cuts at: 0 672 882 1683
Size: 672 210 801
Fragments arranged by size:
801 672 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 227 259 1148 1180 1429 1461 1683
Size: 227 32 889 32 249 32 222
Fragments arranged by size:
889 249 227 222 32 32 32
HincII GTy'rAC
Cuts at: 0 63 1683
Size: 63 1620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1472 1683
Size: 1472 211
MslI CAynn'nnrTG
Cuts at: 0 864 1345 1683
Size: 864 481 338
Fragments arranged by size:
864 481 338
MspA1I CmG'CkG
Cuts at: 0 30 1234 1272 1683
Size: 30 1204 38 411
Fragments arranged by size:
1204 411 38 30
NdeI CA'TA_TG
Cuts at: 0 545 1683
Size: 545 1138
NsiI A_TGCA'T
Cuts at: 0 1446 1683
Size: 1446 237
NspI r_CATG'y
Cuts at: 0 1290 1683
Size: 1290 393
PflMI CCAn_nnn'nTGG
Cuts at: 0 1055 1683
Size: 1055 628
PmlI CAC'GTG
Cuts at: 0 107 1683
Size: 107 1576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 1683
Size: 823 32 828
Fragments arranged by size:
828 823 32
PpuMI rG'GwC_Cy
Cuts at: 0 1176 1647 1683
Size: 1176 471 36
Fragments arranged by size:
1176 471 36
PspOMI G'GGCC_C
Cuts at: 0 75 299 1495 1576 1683
Size: 75 224 1196 81 107
Fragments arranged by size:
1196 224 107 81 75
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 592 624 1683
Size: 592 32 1059
Fragments arranged by size:
1059 592 32
PstI C_TGCA'G
Cuts at: 0 986 1271 1683
Size: 986 285 412
Fragments arranged by size:
986 412 285
PvuII CAG'CTG
Cuts at: 0 30 1272 1683
Size: 30 1242 411
Fragments arranged by size:
1242 411 30
SacII CC_GC'GG
Cuts at: 0 1235 1683
Size: 1235 448
SanDI GG'GwC_CC
Cuts at: 0 1176 1683
Size: 1176 507
SfcI C'TryA_G
Cuts at: 0 982 1267 1617 1683
Size: 982 285 350 66
Fragments arranged by size:
982 350 285 66
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1584 1683
Size: 1584 99
SmlI C'TyrA_G
Cuts at: 0 526 1500 1683
Size: 526 974 183
Fragments arranged by size:
974 526 183
SphI G_CATG'C
Cuts at: 0 1290 1683
Size: 1290 393
SspI AAT'ATT
Cuts at: 0 426 1683
Size: 426 1257
StuI AGG'CCT
Cuts at: 0 703 1683
Size: 703 980
StyI C'CwwG_G
Cuts at: 0 69 269 1491 1683
Size: 69 200 1222 192
Fragments arranged by size:
1222 200 192 69
TatI w'GTAC_w
Cuts at: 0 1193 1457 1683
Size: 1193 264 226
Fragments arranged by size:
1193 264 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 394 426 953 985 1683
Size: 394 32 527 32 698
Fragments arranged by size:
698 527 394 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 739 1683
Size: 739 944
Enzymes that do cut:
AccI AcuI AfeI AflII AhdI AleI AlwNI ApaI
ApoI AseI AvaI AvrII BanII BbsI BfrBI BglI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BssHII BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EciI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII MmeI MslI
MspA1I NdeI NsiI NspI PflMI PmlI PpiI PpuMI
PspOMI PsrI PstI PvuII SacII SanDI SfcI SfiI
SmlI SphI SspI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AlfI AloI
ApaLI AscI AsiSI BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BglII BmgBI BmtI BpuEI BsaBI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BsrGI BssSI BstZ17I ClaI CspCI DraI DrdI EagI
EarI EcoICRI EcoRI EcoRV FalI FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PciI
PfoI PmeI PshAI PsiI PspXI PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI ZraI