(Circular) (Six-base) MAPSORT of: pFN21AA0597.seq Check: 8142 from: 1 to: 7435 pFN21AA0597 7435 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5245 278 Size: 53 83 186 936 3709 2468 Fragments arranged by size: 3709 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4778 1937 Size: 2841 4594 Acc65I G'GTAC_C Cuts at: 4756 4756 Size: 7435 AclI AA'CG_TT Cuts at: 1563 5562 5935 1563 Size: 3999 373 3063 Fragments arranged by size: 3999 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2832 3584 3671 5498 6882 7150 1632 Size: 1200 752 87 1827 1384 268 1917 Fragments arranged by size: 1917 1827 1384 1200 752 268 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7238 AflIII A'CryG_T Cuts at: 1203 2869 2974 6334 7311 1203 Size: 1666 105 3360 977 1327 Fragments arranged by size: 3360 1666 1327 977 105 AgeI A'CCGG_T Cuts at: 6266 6266 Size: 7435 AhdI GACnn_n'nnGTC Cuts at: 3350 6164 3350 Size: 2814 4621 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7435 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2196 1184 Size: 1012 6423 AlwNI CAG_nnn'CTG Cuts at: 3649 5178 6750 3649 Size: 1529 1572 4334 Fragments arranged by size: 4334 1572 1529 ApaLI G'TGCA_C Cuts at: 2458 5492 6648 2458 Size: 3034 1156 3245 Fragments arranged by size: 3245 3034 1156 ApoI r'AATT_y Cuts at: 1493 2416 3077 3370 3886 4744 4970 5122 7231 Size: 923 661 293 516 858 226 152 2109 Cuts at: 7231 1493 Size: 1697 Fragments arranged by size: 2109 1697 923 858 661 516 293 226 152 AseI AT'TA_AT Cuts at: 160 1032 5989 160 Size: 872 4957 1606 Fragments arranged by size: 4957 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 AvaI C'yCGr_G Cuts at: 1870 1943 3584 4760 1870 Size: 73 1641 1176 4545 Fragments arranged by size: 4545 1641 1176 73 BamHI G'GATC_C Cuts at: 4765 5132 4765 Size: 367 7068 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4756 6211 618 Size: 325 217 627 2969 1455 1842 Fragments arranged by size: 2969 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2378 2767 4029 4215 4754 1391 Size: 987 389 1262 186 539 4072 Fragments arranged by size: 4072 1262 987 539 389 186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2028 2866 3292 3910 4166 928 Size: 971 129 838 426 618 256 4197 Fragments arranged by size: 4197 971 838 618 426 256 129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5626 5660 1903 Size: 34 3689 34 3678 Fragments arranged by size: 3689 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 5327 6537 3680 Size: 1647 1210 4578 Fragments arranged by size: 4578 1647 1210 BclI T'GATC_A Cuts at: 1548 2620 4152 1548 Size: 1072 1532 4831 Fragments arranged by size: 4831 1532 1072 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2218 6046 136 Size: 107 122 71 631 605 546 3828 1525 Fragments arranged by size: 3828 1525 631 605 546 122 107 71 BglII A'GATC_T Cuts at: 3086 7430 3086 Size: 4344 3091 BlpI GC'TnA_GC Cuts at: 4852 4852 Size: 7435 Bme1580I G_kGCm'C Cuts at: 1790 2462 4297 5496 6652 1790 Size: 672 1835 1199 1156 2573 Fragments arranged by size: 2573 1835 1199 1156 672 BmgBI CAC'GTC Cuts at: 1318 2457 2977 1318 Size: 1139 520 5776 Fragments arranged by size: 5776 1139 520 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3110 4300 5166 Size: 416 53 83 399 24 1693 1190 866 Cuts at: 5166 6124 442 Size: 958 1753 Fragments arranged by size: 1753 1693 1190 958 866 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2187 2568 3237 3549 3824 4209 6095 1239 Size: 948 381 669 312 275 385 1886 2579 Fragments arranged by size: 2579 1886 948 669 385 381 312 275 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5560 6425 6723 5560 Size: 865 298 6272 Fragments arranged by size: 6272 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3133 3714 4052 4149 6098 882 Size: 617 1634 581 338 97 1949 2219 Fragments arranged by size: 2219 1949 1634 617 581 338 97 BsaAI yAC'GTr Cuts at: 493 1204 3503 5226 493 Size: 711 2299 1723 2702 Fragments arranged by size: 2702 2299 1723 711 BsaBI GATnn'nnATC Cuts at: 3390 7429 3390 Size: 4039 3396 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5242 5624 6259 275 Size: 53 83 186 936 3709 382 635 1451 Fragments arranged by size: 3709 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 5868 6266 6540 6687 7098 4395 Size: 1473 398 274 147 411 4732 Fragments arranged by size: 4732 1473 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3296 3326 3840 3870 1178 Size: 30 165 30 1893 30 514 30 4743 Fragments arranged by size: 4743 1893 514 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2711 2714 2717 2729 4257 1186 Size: 1525 3 3 12 1528 4364 Fragments arranged by size: 4364 1528 1525 12 3 3 BseYI C'CCAG_C Cuts at: 6638 7134 6638 Size: 496 6939 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2622 1163 Size: 573 886 5976 Fragments arranged by size: 5976 886 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4885 5646 6674 1540 Size: 275 186 2884 761 1028 2301 Fragments arranged by size: 2884 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2378 2462 2521 4029 5496 5581 6652 2378 Size: 84 59 1508 1467 85 1071 3161 Fragments arranged by size: 3161 1508 1467 1071 85 84 59 BsmI GAATG_Cn' Cuts at: 2123 2471 2791 2838 4946 5039 2123 Size: 348 320 47 2108 93 4519 Fragments arranged by size: 4519 2108 348 320 93 47 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2378 2462 2521 2767 4029 4215 4297 Size: 399 588 84 59 246 1262 186 82 Cuts at: 4297 4754 5496 5581 6652 1391 Size: 457 742 85 1071 2174 Fragments arranged by size: 2174 1262 1071 742 588 457 399 246 186 85 84 82 59 BspEI T'CCGG_A Cuts at: 4395 7098 4395 Size: 2703 4732 BspHI T'CATG_A Cuts at: 4149 4155 5324 7054 4149 Size: 6 1169 1730 4530 Fragments arranged by size: 4530 1730 1169 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4790 844 Size: 3946 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5322 1394 Size: 429 3499 3507 Fragments arranged by size: 3507 3499 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4320 5930 6104 65 Size: 1183 3072 1610 174 1396 Fragments arranged by size: 3072 1610 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6079 6266 1700 Size: 110 4269 187 2869 Fragments arranged by size: 4269 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7435 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7435 BssSI C'ACGA_G Cuts at: 3637 4667 5495 6507 3637 Size: 1030 828 1012 4565 Fragments arranged by size: 4565 1030 1012 828 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7435 BstBI TT'CG_AA Cuts at: 6239 6239 Size: 7435 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7435 BstYI r'GATC_y Cuts at: 1972 3086 3697 4765 5132 5517 5534 6986 7072 Size: 1114 611 1068 367 385 17 1452 86 Cuts at: 7072 7084 7430 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1114 1068 611 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4104 4244 4104 Size: 140 7295 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 2509 4280 513 Size: 552 939 9 496 1771 3668 Fragments arranged by size: 3668 1771 939 552 496 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6696 Fragments arranged by size: 6696 683 56 BtsI GCAGTG_nn' Cuts at: 2265 2658 3650 4951 5745 5765 2265 Size: 393 992 1301 794 20 3935 Fragments arranged by size: 3935 1301 992 794 393 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7435 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7400 DraI TTT'AAA Cuts at: 2892 3885 4739 2892 Size: 993 854 5588 Fragments arranged by size: 5588 993 854 DraIII CAC_nnn'GTG Cuts at: 2466 2468 2466 Size: 2 7433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6442 809 Size: 507 5126 1802 Fragments arranged by size: 5126 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3979 4882 5771 Size: 54 1425 50 275 18 2149 903 889 Cuts at: 5771 7400 8 Size: 1629 43 Fragments arranged by size: 2149 1629 1425 903 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4882 1537 Size: 275 3070 4090 Fragments arranged by size: 4090 3070 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2688 2808 5365 1353 Size: 1335 120 2557 3423 Fragments arranged by size: 3423 2557 1335 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4595 6022 6396 6542 387 Size: 1064 138 2972 34 1427 374 146 1280 Fragments arranged by size: 2972 1427 1280 1064 374 146 138 34 EcoICRI GAG'CTC Cuts at: 2376 4027 2376 Size: 1651 5784 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 2568 2832 3237 3549 3584 3671 Size: 393 555 381 264 405 312 35 87 Cuts at: 3671 3824 4209 5498 6095 6882 7150 1239 Size: 153 385 1289 597 787 268 1524 Fragments arranged by size: 1524 1289 787 597 555 405 393 385 381 312 268 264 153 87 35 EcoNI CCTnn'n_nnAGG Cuts at: 4506 4506 Size: 7435 EcoO109I rG'GnC_Cy Cuts at: 3851 4343 3851 Size: 492 6943 EcoRI G'AATT_C Cuts at: 4744 5122 4744 Size: 378 7057 EcoRV GAT'ATC Cuts at: 3190 3190 Size: 7435 FspI TGC'GCA Cuts at: 5941 5941 Size: 7435 HaeII r_GCGC'y Cuts at: 1678 4621 6582 1678 Size: 2943 1961 2531 Fragments arranged by size: 2943 2531 1961 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2390 2422 3037 3069 3336 Size: 32 135 32 818 32 615 32 267 Cuts at: 3336 3368 4330 4362 6076 6108 6150 6182 1373 Size: 32 962 32 1714 32 42 32 2626 Fragments arranged by size: 2626 1714 962 818 615 267 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2409 3760 4661 4779 5025 669 Size: 988 281 471 1351 901 118 246 3079 Fragments arranged by size: 3079 1351 988 901 471 281 246 118 HindIII A'AGCT_T Cuts at: 748 748 Size: 7435 HpaI GTT'AAC Cuts at: 3760 5025 3760 Size: 1265 6170 KpnI G_GTAC'C Cuts at: 4760 4760 Size: 7435 MfeI C'AATT_G Cuts at: 5034 5034 Size: 7435 MluI A'CGCG_T Cuts at: 7311 7311 Size: 7435 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2511 6549 6733 1305 Size: 1206 4038 184 2007 Fragments arranged by size: 4038 2007 1206 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5613 Fragments arranged by size: 5613 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2079 2574 4154 5393 5752 5911 518 Size: 648 913 495 1580 1239 359 159 2042 Fragments arranged by size: 2042 1580 1239 913 648 495 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2642 4037 4851 5528 6676 6921 1606 Size: 409 627 1395 814 677 1148 245 2120 Fragments arranged by size: 2120 1395 1148 814 677 627 409 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2509 4280 513 Size: 552 939 505 1771 3668 Fragments arranged by size: 3668 1771 939 552 505 NdeI CA'TA_TG Cuts at: 387 2027 387 Size: 1640 5795 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7435 NotI GC'GGCC_GC Cuts at: 4882 4882 Size: 7435 NspI r_CATG'y Cuts at: 1132 1229 2340 2540 2873 3776 4313 4793 6338 Size: 97 1111 200 333 903 537 480 1545 Cuts at: 6338 1132 Size: 2229 Fragments arranged by size: 2229 1545 1111 903 537 480 333 200 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7435 PciI A'CATG_T Cuts at: 2869 6334 2869 Size: 3465 3970 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7435 PmeI GTTT'AAAC Cuts at: 4739 4739 Size: 7435 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3567 3599 5504 5536 7017 7049 3567 Size: 32 1905 32 1481 32 3953 Fragments arranged by size: 3953 1905 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4343 4343 Size: 7435 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7435 PsiI TTA'TAA Cuts at: 5005 5005 Size: 7435 PspXI vC'TCGA_Gb Cuts at: 3584 3584 Size: 7435 PstI C_TGCA'G Cuts at: 830 4787 830 Size: 3957 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7435 PvuII CAG'CTG Cuts at: 2642 4037 2642 Size: 1395 6040 SacI G_AGCT'C Cuts at: 2378 4029 2378 Size: 1651 5784 SacII CC_GC'GG Cuts at: 2016 2016 Size: 7435 SalI G'TCGA_C Cuts at: 1936 4777 1936 Size: 2841 4594 SapI GCTCTTCn'nnn_ Cuts at: 2808 2808 Size: 7435 SbfI CC_TGCA'GG Cuts at: 4787 4787 Size: 7435 ScaI AGT'ACT Cuts at: 5683 7395 5683 Size: 1712 5723 SexAI A'CCwGG_T Cuts at: 3346 3346 Size: 7435 SfcI C'TryA_G Cuts at: 826 1045 3553 4783 5918 6599 6790 826 Size: 219 2508 1230 1135 681 191 1471 Fragments arranged by size: 2508 1471 1230 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7435 SmaI CCC'GGG Cuts at: 1872 4762 1872 Size: 2890 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3584 5539 6440 6702 820 Size: 197 926 1641 1955 901 262 1553 Fragments arranged by size: 1955 1641 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3503 5226 493 Size: 3010 1723 2702 Fragments arranged by size: 3010 2702 1723 SpeI A'CTAG_T Cuts at: 152 2854 152 Size: 2702 4733 SphI G_CATG'C Cuts at: 1132 2340 2540 3776 4313 4793 1132 Size: 1208 200 1236 537 480 3774 Fragments arranged by size: 3774 1236 1208 537 480 200 SspI AAT'ATT Cuts at: 5 52 5359 5 Size: 47 5307 2081 Fragments arranged by size: 5307 2081 47 StuI AGG'CCT Cuts at: 1517 3000 3813 1517 Size: 1483 813 5139 Fragments arranged by size: 5139 1483 813 StyI C'CwwG_G Cuts at: 513 1065 2004 2509 3198 4254 4280 4874 513 Size: 552 939 505 689 1056 26 594 3074 Fragments arranged by size: 3074 1056 939 689 594 552 505 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2031 2817 2869 5462 5479 5632 5817 Size: 1255 451 786 52 2593 17 153 185 Cuts at: 5817 6262 325 Size: 445 1498 Fragments arranged by size: 2593 1498 1255 786 451 445 185 153 52 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2087 3397 5681 Size: 274 80 33 51 1443 110 1310 2284 Cuts at: 5681 7393 96 Size: 1712 138 Fragments arranged by size: 2284 1712 1443 1310 274 138 110 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4101 4133 4287 4319 7395 7427 4101 Size: 32 154 32 3076 32 4109 Fragments arranged by size: 4109 3076 154 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7435 XbaI T'CTAG_A Cuts at: 4771 4771 Size: 7435 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4287 4287 Size: 7435 XhoI C'TCGA_G Cuts at: 1943 3584 1943 Size: 1641 5794 XmaI C'CCGG_G Cuts at: 1870 4760 1870 Size: 2890 4545 XmnI GAAnn'nnTTC Cuts at: 3813 5564 3813 Size: 1751 5684 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5243 276 Size: 53 83 186 936 3709 2468 Fragments arranged by size: 3709 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SanDI SfiI SfoI SrfI SwaI