Restriction map for
pFN21AA0594
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp718I AspI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BclI BglI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HindIII KpnI KspI
MamI MluI MluNI MroI MscI
MvnI NaeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI SexAI SfiI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 21 GACGTC GACGT^C
AccI 3211 GTCTAC GT^[AC][GT]AC
AhdI 67 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1720 GATCC GGATC(4/5)
AocI 538 CCTCAGG CC^T[ACGT]AGG
AseI 2815 ATTAAT AT^TAAT
AsnI 2815 ATTAAT AT^TAAT
AspEI 67 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 3275 GGCGCC G^G[CT][GA]CC
BbeI 3275 GGCGCC GGCGC^C
BciVI 2169 GGATAC GTATCC(6/5)
BcnI 134 CCGGG CC^[GC]GG
BglII 1654 AGATCT A^GATCT
BpmI 2047 CTGGAG CTGGAG(16/14)
BsgI 2489 GTGCAG GTGCAG(16/14)
BsmBI 1221 GAGACG CGTCTC(1/5)
BspHI 1758 TCATGA T^CATGA
BspLU11I 919 ACATGT A^CATGT
BspMI 300 ACCTGC ACCTGC(4/8)
BstXI 3238 CCAAACACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1654 AGATCT [GA]^GATC[CT]
Bsu36I 538 CCTCAGG CC^T[ACGT]AGG
DraII 80 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 2366 TGGCCG [CT]^GGCC[GA]
Eam1105I 67 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 538 CCTCAGG CC^T[ACGT]AGG
EcoNI 1569 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 80 AGGTCCC [GA]G^G[ACGT]CC[CT]
HaeII 3275 GGCGCC [GA]GCGC^[CT]
HapII 134 CCGG C^CGG
HgaI 948 GCGTC GACGC(5/10)
HpaI 2706 GTTAAC GTT^AAC
HpaII 134 CCGG C^CGG
KasI 3275 GGCGCC G^GCGCC
MflI 1654 AGATCT [GA]^GATC[CT]
MspI 134 CCGG C^CGG
NarI 3275 GGCGCC GG^CGCC
NciI 134 CCGGG CC^[GC]GG
NspI 919 ACATGT [GA]CATG^[CT]
PaeR7I 673 CTCGAG C^TCGAG
PpuMI 80 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2815 ATTAAT AT^TAAT
PvuII 112 CAGCTG CAG^CTG
RcaI 1758 TCATGA T^CATGA
SacI 53 GAGCTC GAGCT^C
ScaI 2938 AGTACT AGT^ACT
SfoI 3275 GGCGCC GGC^GCC
SpeI 858 ACTAGT A^CTAGT
SspI 505 AATATT AAT^ATT
XhoI 673 CTCGAG C^TCGAG
XhoII 1654 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 21 GACGTC G[GA]^CG[CT]C
3275 GGCGCC
AflII 651 CTTAAG C^TTAAG
1524 CTTAAG
AflIII 919 ACATGT A^C[GA][CT]GT
1385 ACACGT
Asp700I 826 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2987 GAAGTGTTTC
AspHI 53 GAGCTC G[AT]GC[AT]^C
2050 GAGCAC
BanII 53 GAGCTC G[GA]GC[CT]^C
1447 GAGCCC
BbsI 18 GAAGAC GAAGAC(2/6)
1955 GAAGAC
BfrI 651 CTTAAG C^TTAAG
1524 CTTAAG
BpuAI 18 GAAGAC GAAGAC(2/6)
1955 GAAGAC
BsaAI 1386 CACGTA [CT]AC^GT[GA]
2027 TACGTG
BsaHI 21 GACGTC G[GA]^CG[CT]C
3275 GGCGCC
BsaI 65 GAGACC GGTCTC(1/5)
676 GAGACC
BsiHKAI 53 GAGCTC G[AT]GC[AT]^C
2050 GAGCAC
BsrI 2118 ACTGG ACTGG(1/-1)
528 CCAGT
BstBI 2912 TTCGAA TT^CGAA
2930 TTCGAA
BstNI 211 CCTGG CC^[AT]GG
2484 CCTGG
Cac8I 1068 GCAGGC GC[ACGT]^[ACGT]GC
1998 GCAAGC
CfoI 1918 GCGC GCG^C
3276 GCGC
EcoRI 99 GAATTC G^AATTC
2909 GAATTC
EcoRII 211 CCTGG ^CC[AT]GG
2484 CCTGG
FauI 108 CCCGC CCCGC(4/6)
1127 CCCGC
HhaI 1918 GCGC GCG^C
3276 GCGC
Hin1I 21 GACGTC G[GA]^CG[CT]C
3275 GGCGCC
HinP1I 1918 GCGC G^CGC
3276 GCGC
HincII 24 GTCAAC GT[CT]^[GA]AC
2706 GTTAAC
HindII 24 GTCAAC GT[CT]^[GA]AC
2706 GTTAAC
MfeI 605 CAATTG C^AATTG
2663 CAATTG
MspA1I 112 CAGCTG C[AC]G^C[GT]G
118 CCGCTG
MunI 605 CAATTG C^AATTG
2663 CAATTG
MvaI 211 CCTGG CC^[AT]GG
2484 CCTGG
NcoI 811 CCATGG C^CATGG
2564 CCATGG
NspV 2912 TTCGAA TT^CGAA
2930 TTCGAA
SapI 1093 GAAGAGC GCTCTTC(1/4)
2191 GAAGAGC
SfuI 2912 TTCGAA TT^CGAA
2930 TTCGAA
XbaI 355 TCTAGA T^CTAGA
1290 TCTAGA
XmnI 826 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2987 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaI AvaII BbvI BfaI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BstF5I
Cfr13I DdeI DpnII DraI DsaI
EarI EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MwoI NdeI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I