(Circular) (Six-base) MAPSORT of: pFN21AA0594.seq Check: 7889 from: 1 to: 8008 pFN21AA0594 8008 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2030 5818 278 Size: 53 83 186 936 494 3788 2468 Fragments arranged by size: 3788 2468 936 494 186 83 53 AccI GT'mk_AC Cuts at: 1937 5217 5351 1937 Size: 3280 134 4594 Fragments arranged by size: 4594 3280 134 Acc65I G'GTAC_C Cuts at: 5329 5329 Size: 8008 AclI AA'CG_TT Cuts at: 1563 6135 6508 1563 Size: 4572 373 3063 Fragments arranged by size: 4572 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3053 3176 4644 5009 6071 7455 7723 1632 Size: 1421 123 1468 365 1062 1384 268 1917 Fragments arranged by size: 1917 1468 1421 1384 1062 365 268 123 AflII C'TTAA_G Cuts at: 820 1017 2656 3529 820 Size: 197 1639 873 5299 Fragments arranged by size: 5299 1639 873 197 AflIII A'CryG_T Cuts at: 1203 2924 3390 6907 7884 1203 Size: 1721 466 3517 977 1327 Fragments arranged by size: 3517 1721 1327 977 466 AgeI A'CCGG_T Cuts at: 6839 6839 Size: 8008 AhdI GACnn_n'nnGTC Cuts at: 2077 6737 2077 Size: 4660 3348 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8008 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8008 AlwNI CAG_nnn'CTG Cuts at: 5751 7323 5751 Size: 1572 6436 ApaLI G'TGCA_C Cuts at: 6065 7221 6065 Size: 1156 6852 ApoI r'AATT_y Cuts at: 1493 2104 2561 3431 3987 4829 4914 4931 5254 Size: 611 457 870 556 842 85 17 323 Cuts at: 5254 5317 5543 5695 7804 1493 Size: 63 226 152 2109 1697 Fragments arranged by size: 2109 1697 870 842 611 556 457 323 226 152 85 63 17 AseI AT'TA_AT Cuts at: 160 1032 4821 6562 160 Size: 872 3789 1741 1606 Fragments arranged by size: 3789 1741 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8008 AvaI C'yCGr_G Cuts at: 1870 1943 2065 2089 2678 5333 1870 Size: 73 122 24 589 2655 4545 Fragments arranged by size: 4545 2655 589 122 73 24 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4515 4548 4515 Size: 33 7975 BamHI G'GATC_C Cuts at: 5338 5705 5338 Size: 367 7641 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5280 5329 6784 618 Size: 325 217 627 3493 49 1455 1842 Fragments arranged by size: 3493 1842 1455 627 325 217 49 BanII G_rGCy'C Cuts at: 1391 2062 3456 5327 1391 Size: 671 1394 1871 4072 Fragments arranged by size: 4072 1871 1394 671 BbeI G_GCGC'C Cuts at: 5284 5284 Size: 8008 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2030 3967 928 Size: 971 131 1937 4969 Fragments arranged by size: 4969 1937 971 131 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6199 6233 1903 Size: 34 4262 34 3678 Fragments arranged by size: 4262 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4168 5900 7110 4168 Size: 1732 1210 5066 Fragments arranged by size: 5066 1732 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8008 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6619 136 Size: 107 122 71 631 605 4947 1525 Fragments arranged by size: 4947 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3659 8003 3659 Size: 4344 3664 BlpI GC'TnA_GC Cuts at: 5425 5425 Size: 8008 Bme1580I G_kGCm'C Cuts at: 1790 6069 7225 1790 Size: 4279 1156 2573 Fragments arranged by size: 4279 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8008 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5739 6697 442 Size: 416 53 83 399 24 4322 958 1753 Fragments arranged by size: 4322 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8008 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3309 3309 Size: 8008 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4073 6668 1239 Size: 2834 2595 2579 Fragments arranged by size: 2834 2595 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4118 4497 6133 6998 7296 4118 Size: 379 1636 865 298 4830 Fragments arranged by size: 4830 1636 865 379 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2064 2675 6671 882 Size: 617 565 611 3996 2219 Fragments arranged by size: 3996 2219 617 611 565 BsaAI yAC'GTr Cuts at: 493 1204 3393 4034 5799 493 Size: 711 2189 641 1765 2702 Fragments arranged by size: 2702 2189 1765 711 641 BsaBI GATnn'nnATC Cuts at: 8002 8002 Size: 8008 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 5281 5815 6197 Size: 53 83 186 936 494 3254 534 382 Cuts at: 6197 6832 275 Size: 635 1451 Fragments arranged by size: 3254 1451 936 635 534 494 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6441 6839 7113 7260 7671 6441 Size: 398 274 147 411 6778 Fragments arranged by size: 6778 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2017 2047 3109 3139 5154 Size: 30 165 30 614 30 1062 30 2015 Cuts at: 5154 5184 1178 Size: 30 4002 Fragments arranged by size: 4002 2015 1062 614 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2579 3130 4549 4956 1186 Size: 1393 551 1419 407 4238 Fragments arranged by size: 4238 1419 1393 551 407 BseYI C'CCAG_C Cuts at: 2043 7211 7707 2043 Size: 5168 496 2344 Fragments arranged by size: 5168 2344 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4515 1163 Size: 573 2779 4656 Fragments arranged by size: 4656 2779 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5458 6219 7247 1540 Size: 275 186 3457 761 1028 2301 Fragments arranged by size: 3457 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2062 4059 6069 6154 7225 2062 Size: 1997 2010 85 1071 2845 Fragments arranged by size: 2845 2010 1997 1071 85 BsmI GAATG_Cn' Cuts at: 5519 5612 5519 Size: 93 7915 BsmBI CGTCTCn'nnnn_ Cuts at: 3220 3220 Size: 8008 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 3456 4059 5327 6069 6154 7225 Size: 399 272 1394 603 1268 742 85 1071 Cuts at: 7225 1391 Size: 2174 Fragments arranged by size: 2174 1394 1268 1071 742 603 399 272 85 BspEI T'CCGG_A Cuts at: 7671 7671 Size: 8008 BspHI T'CATG_A Cuts at: 3763 5897 7627 3763 Size: 2134 1730 4144 Fragments arranged by size: 4144 2134 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2314 5363 844 Size: 1470 3049 3489 Fragments arranged by size: 3489 3049 1470 BsrBI CCG'CTC Cuts at: 1394 1823 5895 1394 Size: 429 4072 3507 Fragments arranged by size: 4072 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6503 6677 65 Size: 1183 5255 174 1396 Fragments arranged by size: 5255 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6652 6839 1700 Size: 110 4842 187 2869 Fragments arranged by size: 4842 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8008 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8008 BssSI C'ACGA_G Cuts at: 6068 7080 6068 Size: 1012 6996 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8008 BstBI TT'CG_AA Cuts at: 4918 4936 6812 4918 Size: 18 1876 6114 Fragments arranged by size: 6114 1876 18 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8008 BstXI CCAn_nnnn'nTGG Cuts at: 5250 5250 Size: 8008 BstYI r'GATC_y Cuts at: 1972 3659 5338 5705 6090 6107 7559 7645 7657 Size: 1687 1679 367 385 17 1452 86 12 Cuts at: 7657 8003 1972 Size: 346 1977 Fragments arranged by size: 1977 1687 1679 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2544 2544 Size: 8008 BtgI C'CryG_G Cuts at: 513 1065 2004 2816 3171 4569 513 Size: 552 939 812 355 1398 3952 Fragments arranged by size: 3952 1398 939 812 552 355 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7269 Fragments arranged by size: 7269 683 56 BtsI GCAGTG_nn' Cuts at: 4014 4862 5524 6318 6338 4014 Size: 848 662 794 20 5684 Fragments arranged by size: 5684 848 794 662 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8008 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7973 DraI TTT'AAA Cuts at: 3436 4158 4800 5259 5312 3436 Size: 722 642 459 53 6132 Fragments arranged by size: 6132 722 642 459 53 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7015 809 Size: 507 5699 1802 Fragments arranged by size: 5699 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4371 5455 6344 Size: 54 1425 50 275 18 2541 1084 889 Cuts at: 6344 7973 8 Size: 1629 43 Fragments arranged by size: 2541 1629 1425 1084 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5455 1537 Size: 275 3643 4090 Fragments arranged by size: 4090 3643 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2342 2484 2880 3093 3274 4185 4191 4512 Size: 989 142 396 213 181 911 6 321 Cuts at: 4512 5938 1353 Size: 1426 3423 Fragments arranged by size: 3423 1426 989 911 396 321 213 181 142 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6595 6969 7115 387 Size: 1064 138 5006 374 146 1280 Fragments arranged by size: 5006 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2060 2060 Size: 8008 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3053 3176 4073 4644 5009 6071 6668 Size: 393 1421 123 897 571 365 1062 597 Cuts at: 6668 7455 7723 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1421 1062 897 787 597 571 393 365 268 123 EcoNI CCTnn'n_nnAGG Cuts at: 3578 3578 Size: 8008 EcoO109I rG'GnC_Cy Cuts at: 2086 2086 Size: 8008 EcoRI G'AATT_C Cuts at: 2104 4914 5317 5695 2104 Size: 2810 403 378 4417 Fragments arranged by size: 4417 2810 403 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4265 4265 Size: 8008 FspI TGC'GCA Cuts at: 6514 6514 Size: 8008 HaeII r_GCGC'y Cuts at: 1678 5284 7155 1678 Size: 3606 1871 2531 Fragments arranged by size: 3606 2531 1871 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2017 2049 2063 2095 4495 Size: 32 135 32 445 32 14 32 2400 Cuts at: 4495 4527 6649 6681 6723 6755 1373 Size: 32 2122 32 42 32 2626 Fragments arranged by size: 2626 2400 2122 445 135 42 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 2031 4713 5352 5598 669 Size: 988 281 93 2682 639 246 3079 Fragments arranged by size: 3079 2682 988 639 281 246 93 HindIII A'AGCT_T Cuts at: 748 748 Size: 8008 HpaI GTT'AAC Cuts at: 4713 5598 4713 Size: 885 7123 KasI G'GCGC_C Cuts at: 5280 5280 Size: 8008 KpnI G_GTAC'C Cuts at: 5333 5333 Size: 8008 MfeI C'AATT_G Cuts at: 2610 4668 5607 2610 Size: 2058 939 5011 Fragments arranged by size: 5011 2058 939 MluI A'CGCG_T Cuts at: 7884 7884 Size: 8008 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2061 2221 2536 7122 7306 1305 Size: 756 160 315 4586 184 2007 Fragments arranged by size: 4586 2007 756 315 184 160 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6186 Fragments arranged by size: 6186 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 3762 4977 5966 6325 6484 518 Size: 648 1008 1588 1215 989 359 159 2042 Fragments arranged by size: 2042 1588 1215 1008 989 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2119 2125 5424 6101 7249 7494 1606 Size: 513 6 3299 677 1148 245 2120 Fragments arranged by size: 3299 2120 1148 677 513 245 6 NarI GG'CG_CC Cuts at: 5281 5281 Size: 8008 NcoI C'CATG_G Cuts at: 513 1065 2004 2816 4569 513 Size: 552 939 812 1753 3952 Fragments arranged by size: 3952 1753 939 812 552 NdeI CA'TA_TG Cuts at: 387 2194 3637 5232 387 Size: 1807 1443 1595 3163 Fragments arranged by size: 3163 1807 1595 1443 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8008 NotI GC'GGCC_GC Cuts at: 5455 5455 Size: 8008 NspI r_CATG'y Cuts at: 1132 1229 2928 5366 6911 1132 Size: 97 1699 2438 1545 2229 Fragments arranged by size: 2438 2229 1699 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8008 PciI A'CATG_T Cuts at: 2924 6907 2924 Size: 3983 4025 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8008 PfoI T'CCnGG_A Cuts at: 2215 2215 Size: 8008 PmeI GTTT'AAAC Cuts at: 5312 5312 Size: 8008 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2341 2373 6077 6109 7590 7622 2341 Size: 32 3704 32 1481 32 2727 Fragments arranged by size: 3704 2727 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2086 2086 Size: 8008 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8008 PsiI TTA'TAA Cuts at: 5578 5578 Size: 8008 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2850 2882 3798 3830 2850 Size: 32 916 32 7028 Fragments arranged by size: 7028 916 32 32 PstI C_TGCA'G Cuts at: 830 5360 830 Size: 4530 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8008 PvuII CAG'CTG Cuts at: 2119 2119 Size: 8008 SacI G_AGCT'C Cuts at: 2062 2062 Size: 8008 SalI G'TCGA_C Cuts at: 1936 5350 1936 Size: 3414 4594 SapI GCTCTTCn'nnn_ Cuts at: 3093 4191 3093 Size: 1098 6910 SbfI CC_TGCA'GG Cuts at: 5360 5360 Size: 8008 ScaI AGT'ACT Cuts at: 4945 6256 7968 4945 Size: 1311 1712 4985 Fragments arranged by size: 4985 1712 1311 SfcI C'TryA_G Cuts at: 826 1045 2711 3774 4329 5356 6491 7172 7363 Size: 219 1666 1063 555 1027 1135 681 191 Cuts at: 7363 826 Size: 1471 Fragments arranged by size: 1666 1471 1135 1063 1027 681 555 219 191 SfoI GGC'GCC Cuts at: 5282 5282 Size: 8008 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8008 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8008 SmaI CCC'GGG Cuts at: 1872 5335 1872 Size: 3463 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2656 2678 3529 4097 4512 6112 Size: 197 926 713 22 851 568 415 1600 Cuts at: 6112 7013 7275 820 Size: 901 262 1553 Fragments arranged by size: 1600 1553 926 901 851 713 568 415 262 197 22 SnaBI TAC'GTA Cuts at: 493 5799 493 Size: 5306 2702 SpeI A'CTAG_T Cuts at: 152 2863 152 Size: 2711 5297 SphI G_CATG'C Cuts at: 1132 5366 1132 Size: 4234 3774 SspI AAT'ATT Cuts at: 5 52 2512 5932 5 Size: 47 2460 3420 2081 Fragments arranged by size: 3420 2460 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8008 StyI C'CwwG_G Cuts at: 513 1065 2004 2287 2816 4569 5447 513 Size: 552 939 283 529 1753 878 3074 Fragments arranged by size: 3074 1753 939 878 552 529 283 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2809 2889 3123 4481 6035 6052 6205 Size: 1255 1229 80 234 1358 1554 17 153 Cuts at: 6205 6390 6835 325 Size: 185 445 1498 Fragments arranged by size: 1554 1498 1358 1255 1229 445 234 185 153 80 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4283 4943 6254 Size: 274 80 33 51 1443 2306 660 1311 Cuts at: 6254 7966 96 Size: 1712 138 Fragments arranged by size: 2306 1712 1443 1311 660 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5154 5186 7968 8000 5154 Size: 32 2782 32 5162 Fragments arranged by size: 5162 2782 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8008 XbaI T'CTAG_A Cuts at: 2360 3295 5344 2360 Size: 935 2049 5024 Fragments arranged by size: 5024 2049 935 XhoI C'TCGA_G Cuts at: 1943 2678 1943 Size: 735 7273 XmaI C'CCGG_G Cuts at: 1870 5333 1870 Size: 3463 4545 XmnI GAAnn'nnTTC Cuts at: 2835 4996 6137 2835 Size: 2161 1141 4706 Fragments arranged by size: 4706 2161 1141 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2028 5816 276 Size: 53 83 186 936 494 3788 2468 Fragments arranged by size: 3788 2468 936 494 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI