(Circular) (Six-base) MAPSORT of: pFN21AA0580.seq Check: 6956 from: 1 to: 9817 pFN21AA0580 9817 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6187 6187 Size: 9817 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7627 278 Size: 53 83 186 936 6091 2468 Fragments arranged by size: 6091 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4820 7160 1937 Size: 2883 2340 4594 Fragments arranged by size: 4594 2883 2340 Acc65I G'GTAC_C Cuts at: 7138 7138 Size: 9817 AclI AA'CG_TT Cuts at: 1563 6655 7944 8317 1563 Size: 5092 1289 373 3063 Fragments arranged by size: 5092 3063 1289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4719 6948 7880 9264 9532 1632 Size: 3087 2229 932 1384 268 1917 Fragments arranged by size: 3087 2229 1917 1384 932 268 AflII C'TTAA_G Cuts at: 820 1017 5182 820 Size: 197 4165 5455 Fragments arranged by size: 5455 4165 197 AflIII A'CryG_T Cuts at: 1203 4939 8716 9693 1203 Size: 3736 3777 977 1327 Fragments arranged by size: 3777 3736 1327 977 AgeI A'CCGG_T Cuts at: 6378 8648 6378 Size: 2270 7547 AhdI GACnn_n'nnGTC Cuts at: 8546 8546 Size: 9817 AleI CACnn'nnGTG Cuts at: 1166 6285 1166 Size: 5119 4698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9817 AlwNI CAG_nnn'CTG Cuts at: 2645 3107 4101 4127 5042 6926 7560 9132 2645 Size: 462 994 26 915 1884 634 1572 3330 Fragments arranged by size: 3330 1884 1572 994 915 634 462 26 ApaI G_GGCC'C Cuts at: 4905 4905 Size: 9817 ApaLI G'TGCA_C Cuts at: 7874 9030 7874 Size: 1156 8661 ApoI r'AATT_y Cuts at: 1493 2488 2686 2968 2983 3037 3065 3289 3521 Size: 995 198 282 15 54 28 224 232 Cuts at: 3521 3629 3810 4397 4595 4613 7126 7352 7504 Size: 108 181 587 198 18 2513 226 152 Cuts at: 7504 9613 1493 Size: 2109 1697 Fragments arranged by size: 2513 2109 1697 995 587 282 232 226 224 198 198 181 152 108 54 28 18 15 AseI AT'TA_AT Cuts at: 160 1032 2058 4842 5874 8371 160 Size: 872 1026 2784 1032 2497 1606 Fragments arranged by size: 2784 2497 1606 1032 1026 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9817 AvaI C'yCGr_G Cuts at: 1870 1943 3198 7142 1870 Size: 73 1255 3944 4545 Fragments arranged by size: 4545 3944 1255 73 BamHI G'GATC_C Cuts at: 7147 7514 7147 Size: 367 9450 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4835 6549 7138 8593 618 Size: 325 217 627 3048 1714 589 1455 1842 Fragments arranged by size: 3048 1842 1714 1455 627 589 325 217 BanII G_rGCy'C Cuts at: 1391 4905 5619 7136 1391 Size: 3514 714 1517 4072 Fragments arranged by size: 4072 3514 1517 714 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2394 2564 4662 5194 6986 928 Size: 971 495 170 2098 532 1792 3759 Fragments arranged by size: 3759 2098 1792 971 532 495 170 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6185 6219 8008 8042 1903 Size: 34 4248 34 1789 34 3678 Fragments arranged by size: 4248 3678 1789 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2175 2430 3186 7709 8919 2175 Size: 255 756 4523 1210 3073 Fragments arranged by size: 4523 3073 1210 756 255 BclI T'GATC_A Cuts at: 1548 2299 2607 7018 1548 Size: 751 308 4411 4347 Fragments arranged by size: 4411 4347 751 308 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6960 8428 136 Size: 107 122 71 631 605 5288 1468 1525 Fragments arranged by size: 5288 1525 1468 631 605 122 107 71 BglII A'GATC_T Cuts at: 4187 4920 9812 4187 Size: 733 4892 4192 Fragments arranged by size: 4892 4192 733 BlpI GC'TnA_GC Cuts at: 5372 7234 5372 Size: 1862 7955 Bme1580I G_kGCm'C Cuts at: 1790 4905 7878 9034 1790 Size: 3115 2973 1156 2573 Fragments arranged by size: 3115 2973 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9817 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4141 4669 4908 Size: 416 53 83 399 24 2724 528 239 Cuts at: 4908 6311 7548 8506 442 Size: 1403 1237 958 1753 Fragments arranged by size: 2724 1753 1403 1237 958 528 416 399 239 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4239 4565 1056 Size: 3183 326 6308 Fragments arranged by size: 6308 3183 326 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3104 3104 Size: 9817 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5513 6000 6212 6872 6932 8477 1239 Size: 4274 487 212 660 60 1545 2579 Fragments arranged by size: 4274 2579 1545 660 487 212 60 Bpu10I CC'TnA_GC Cuts at: 3533 3533 Size: 9817 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3471 3735 4816 4953 7942 8807 9105 3471 Size: 264 1081 137 2989 865 298 4183 Fragments arranged by size: 4183 2989 1081 865 298 264 137 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3005 4527 8480 882 Size: 617 1506 1522 3953 2219 Fragments arranged by size: 3953 2219 1522 1506 617 BsaAI yAC'GTr Cuts at: 493 1204 7608 493 Size: 711 6404 2702 Fragments arranged by size: 6404 2702 711 BsaBI GATnn'nnATC Cuts at: 9811 9811 Size: 9817 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7624 8006 8641 275 Size: 53 83 186 936 6091 382 635 1451 Fragments arranged by size: 6091 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4471 4529 6291 6378 8250 8648 8922 9069 9480 Size: 58 1762 87 1872 398 274 147 411 Cuts at: 9480 4471 Size: 4808 Fragments arranged by size: 4808 1872 1762 411 398 274 147 87 58 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4500 4524 4530 4554 4916 Size: 30 165 30 3097 24 6 24 362 Cuts at: 4916 4946 1178 Size: 30 6049 Fragments arranged by size: 6049 3097 362 165 30 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2436 2993 3474 3698 1186 Size: 1250 557 481 224 7305 Fragments arranged by size: 7305 1250 557 481 224 BseYI C'CCAG_C Cuts at: 4574 6513 6609 9020 9516 4574 Size: 1939 96 2411 496 4875 Fragments arranged by size: 4875 2411 1939 496 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2139 4046 4125 4194 1163 Size: 573 403 1907 79 69 6786 Fragments arranged by size: 6786 1907 573 403 79 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7267 8028 9056 1540 Size: 275 186 5266 761 1028 2301 Fragments arranged by size: 5266 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5619 6858 7878 7963 9034 5619 Size: 1239 1020 85 1071 6402 Fragments arranged by size: 6402 1239 1071 1020 85 BsmI GAATG_Cn' Cuts at: 2654 3269 5181 5788 6724 7328 7421 2654 Size: 615 1912 607 936 604 93 5050 Fragments arranged by size: 5050 1912 936 615 607 604 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4905 5619 6858 7136 7878 7963 9034 Size: 399 3115 714 1239 278 742 85 1071 Cuts at: 9034 1391 Size: 2174 Fragments arranged by size: 3115 2174 1239 1071 742 714 399 278 85 BspEI T'CCGG_A Cuts at: 9480 9480 Size: 9817 BspHI T'CATG_A Cuts at: 7706 9436 7706 Size: 1730 8087 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5327 6187 7172 844 Size: 4483 860 985 3489 Fragments arranged by size: 4483 3489 985 860 BsrBI CCG'CTC Cuts at: 1394 1823 7704 1394 Size: 429 5881 3507 Fragments arranged by size: 5881 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4255 8312 8486 65 Size: 1183 3007 4057 174 1396 Fragments arranged by size: 4057 3007 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6378 8461 8648 1700 Size: 110 4568 2083 187 2869 Fragments arranged by size: 4568 2869 2083 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9817 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9817 BssSI C'ACGA_G Cuts at: 7877 8889 7877 Size: 1012 8805 BstAPI GCAn_nnn'nTGC Cuts at: 794 3107 4585 794 Size: 2313 1478 6026 Fragments arranged by size: 6026 2313 1478 BstBI TT'CG_AA Cuts at: 3572 6874 8621 3572 Size: 3302 1747 4768 Fragments arranged by size: 4768 3302 1747 BstEII G'GTnAC_C Cuts at: 1184 2168 1184 Size: 984 8833 BstXI CCAn_nnnn'nTGG Cuts at: 2093 6513 2093 Size: 4420 5397 BstYI r'GATC_y Cuts at: 1972 4123 4187 4920 5631 7147 7514 7899 7916 Size: 2151 64 733 711 1516 367 385 17 Cuts at: 7916 9368 9454 9466 9812 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2151 1977 1516 1452 733 711 385 367 346 86 64 17 12 BtgI C'CryG_G Cuts at: 513 1065 5281 6804 6834 513 Size: 552 4216 1523 30 3496 Fragments arranged by size: 4216 3496 1523 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9078 Fragments arranged by size: 9078 683 56 BtsI GCAGTG_nn' Cuts at: 2466 3228 5600 6280 6292 6844 7333 8127 8147 Size: 762 2372 680 12 552 489 794 20 Cuts at: 8147 2466 Size: 4136 Fragments arranged by size: 4136 2372 794 762 680 552 489 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9817 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2343 2378 529 Size: 35 1779 35 7968 Fragments arranged by size: 7968 1779 35 35 DraI TTT'AAA Cuts at: 4893 5524 7121 4893 Size: 631 1597 7589 Fragments arranged by size: 7589 1597 631 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8824 809 Size: 507 7508 1802 Fragments arranged by size: 7508 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6629 7264 8153 Size: 54 1425 50 275 18 4799 635 889 Cuts at: 8153 9782 8 Size: 1629 43 Fragments arranged by size: 4799 1629 1425 889 635 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7264 1537 Size: 275 5452 4090 Fragments arranged by size: 5452 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3026 3675 4738 4749 5072 5703 6147 6174 Size: 1673 649 1063 11 323 631 444 27 Cuts at: 6174 6245 6422 7747 1353 Size: 71 177 1325 3423 Fragments arranged by size: 3423 1673 1325 1063 649 631 444 323 177 71 27 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8404 8778 8924 387 Size: 1064 138 6815 374 146 1280 Fragments arranged by size: 6815 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5617 5617 Size: 9817 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4719 5513 6000 6212 6872 6932 6948 Size: 393 3087 794 487 212 660 60 16 Cuts at: 6948 7880 8477 9264 9532 1239 Size: 932 597 787 268 1524 Fragments arranged by size: 3087 1524 932 794 787 660 597 487 393 268 212 60 16 EcoNI CCTnn'n_nnAGG Cuts at: 6111 6111 Size: 9817 EcoO109I rG'GnC_Cy Cuts at: 5866 5866 Size: 9817 EcoRI G'AATT_C Cuts at: 2983 7126 7504 2983 Size: 4143 378 5296 Fragments arranged by size: 5296 4143 378 EcoRV GAT'ATC Cuts at: 3782 6045 3782 Size: 2263 7554 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6274 6953 6274 Size: 679 9138 FspI TGC'GCA Cuts at: 8323 8323 Size: 9817 HaeII r_GCGC'y Cuts at: 1678 8964 1678 Size: 7286 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2426 2458 2551 2583 2972 Size: 32 135 32 854 32 93 32 389 Cuts at: 2972 3004 3464 3496 4121 4153 4500 4532 6439 Size: 32 460 32 625 32 347 32 1907 Cuts at: 6439 6471 6785 6817 8458 8490 8532 8564 1373 Size: 32 314 32 1641 32 42 32 2626 Fragments arranged by size: 2626 1907 1641 854 625 460 389 347 314 135 93 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2889 5063 5710 7161 7407 669 Size: 988 281 951 2174 647 1451 246 3079 Fragments arranged by size: 3079 2174 1451 988 951 647 281 246 HindIII A'AGCT_T Cuts at: 748 5518 748 Size: 4770 5047 HpaI GTT'AAC Cuts at: 7407 7407 Size: 9817 KpnI G_GTAC'C Cuts at: 7142 7142 Size: 9817 MfeI C'AATT_G Cuts at: 5322 7416 5322 Size: 2094 7723 MluI A'CGCG_T Cuts at: 9693 9693 Size: 9817 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2343 4116 5277 6530 8931 9115 1305 Size: 1038 1773 1161 1253 2401 184 2007 Fragments arranged by size: 2401 2007 1773 1253 1161 1038 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6631 10 Size: 54 1425 343 4799 3196 Fragments arranged by size: 4799 3196 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2085 5396 5481 5781 6285 6618 7775 Size: 648 919 3311 85 300 504 333 1157 Cuts at: 7775 8134 8293 518 Size: 359 159 2042 Fragments arranged by size: 3311 2042 1157 919 648 504 359 333 300 159 85 MspA1I CmG'CkG Cuts at: 1606 5406 6266 6638 7233 7910 9058 9303 1606 Size: 3800 860 372 595 677 1148 245 2120 Fragments arranged by size: 3800 2120 1148 860 677 595 372 245 NcoI C'CATG_G Cuts at: 513 1065 5281 6834 513 Size: 552 4216 1553 3496 Fragments arranged by size: 4216 3496 1553 552 NdeI CA'TA_TG Cuts at: 387 4338 387 Size: 3951 5866 NheI G'CTAG_C Cuts at: 1052 4235 4561 1052 Size: 3183 326 6308 Fragments arranged by size: 6308 3183 326 NotI GC'GGCC_GC Cuts at: 7264 7264 Size: 9817 NspI r_CATG'y Cuts at: 1132 1229 3502 7175 8720 1132 Size: 97 2273 3673 1545 2229 Fragments arranged by size: 3673 2273 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3076 1348 Size: 1728 8089 PciI A'CATG_T Cuts at: 8716 8716 Size: 9817 PflMI CCAn_nnn'nTGG Cuts at: 1108 5089 5794 6638 6850 1108 Size: 3981 705 844 212 4075 Fragments arranged by size: 4075 3981 844 705 212 PfoI T'CCnGG_A Cuts at: 5490 5490 Size: 9817 PmeI GTTT'AAAC Cuts at: 7121 7121 Size: 9817 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7886 7918 9399 9431 7886 Size: 32 1481 32 8272 Fragments arranged by size: 8272 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5866 5866 Size: 9817 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9817 PsiI TTA'TAA Cuts at: 6035 7387 6035 Size: 1352 8465 PspOMI G'GGCC_C Cuts at: 4901 4901 Size: 9817 PstI C_TGCA'G Cuts at: 830 2145 2473 6287 7169 830 Size: 1315 328 3814 882 3478 Fragments arranged by size: 3814 3478 1315 882 328 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9817 PvuII CAG'CTG Cuts at: 5406 6638 5406 Size: 1232 8585 SacI G_AGCT'C Cuts at: 5619 5619 Size: 9817 SalI G'TCGA_C Cuts at: 1936 7159 1936 Size: 5223 4594 SapI GCTCTTCn'nnn_ Cuts at: 6147 6147 Size: 9817 SbfI CC_TGCA'GG Cuts at: 7169 7169 Size: 9817 ScaI AGT'ACT Cuts at: 8065 9777 8065 Size: 1712 8105 SexAI A'CCwGG_T Cuts at: 6240 6240 Size: 9817 SfcI C'TryA_G Cuts at: 826 1045 2141 2163 2469 3964 4461 6025 6283 Size: 219 1096 22 306 1495 497 1564 258 Cuts at: 6283 7165 8300 8981 9172 826 Size: 882 1135 681 191 1471 Fragments arranged by size: 1564 1495 1471 1135 1096 882 681 497 306 258 219 191 22 SfiI GGCCn_nnn'nGGCC Cuts at: 6960 6960 Size: 9817 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9817 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9817 SmaI CCC'GGG Cuts at: 1872 7144 1872 Size: 5272 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3198 3486 3750 4795 4968 5182 Size: 197 926 1255 288 264 1045 173 214 Cuts at: 5182 7921 8822 9084 820 Size: 2739 901 262 1553 Fragments arranged by size: 2739 1553 1255 1045 926 901 288 264 262 214 197 173 SnaBI TAC'GTA Cuts at: 493 7608 493 Size: 7115 2702 SpeI A'CTAG_T Cuts at: 152 5840 152 Size: 5688 4129 SphI G_CATG'C Cuts at: 1132 7175 1132 Size: 6043 3774 SspI AAT'ATT Cuts at: 5 52 2530 5892 6100 6742 7741 5 Size: 47 2478 3362 208 642 999 2081 Fragments arranged by size: 3362 2478 2081 999 642 208 47 StuI AGG'CCT Cuts at: 1517 6963 1517 Size: 5446 4371 StyI C'CwwG_G Cuts at: 513 1065 4209 5281 5799 6726 6834 7256 513 Size: 552 3144 1072 518 927 108 422 3074 Fragments arranged by size: 3144 3074 1072 927 552 518 422 108 SwaI ATTT'AAAT Cuts at: 4893 4893 Size: 9817 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3505 3942 5978 7844 7861 8014 8199 Size: 1255 1925 437 2036 1866 17 153 185 Cuts at: 8199 8644 325 Size: 445 1498 Fragments arranged by size: 2036 1925 1866 1498 1255 445 437 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6187 6296 8063 Size: 274 80 33 51 1443 4210 109 1767 Cuts at: 8063 9775 96 Size: 1712 138 Fragments arranged by size: 4210 1767 1712 1443 274 138 109 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3822 3854 4524 4556 9777 9809 3822 Size: 32 670 32 5221 32 3830 Fragments arranged by size: 5221 3830 670 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9817 XbaI T'CTAG_A Cuts at: 4480 7153 4480 Size: 2673 7144 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4128 4128 Size: 9817 XhoI C'TCGA_G Cuts at: 1943 3198 1943 Size: 1255 8562 XmaI C'CCGG_G Cuts at: 1870 7142 1870 Size: 5272 4545 XmnI GAAnn'nnTTC Cuts at: 2686 3267 3289 6254 6655 7946 2686 Size: 581 22 2965 401 1291 4557 Fragments arranged by size: 4557 2965 1291 581 401 22 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7625 276 Size: 53 83 186 936 6091 2468 Fragments arranged by size: 6091 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfoI SrfI