(Circular) (Six-base) MAPSORT of: pFN21AA0556.seq Check: 7069 from: 1 to: 9559 pFN21AA0556 9559 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5745 5745 Size: 9559 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6369 7369 278 Size: 53 83 186 936 4833 1000 2468 Fragments arranged by size: 4833 2468 1000 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4952 6902 1937 Size: 3015 1950 4594 Fragments arranged by size: 4594 3015 1950 Acc65I G'GTAC_C Cuts at: 6880 6880 Size: 9559 AclI AA'CG_TT Cuts at: 1563 7686 8059 1563 Size: 6123 373 3063 Fragments arranged by size: 6123 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 2367 5624 7622 9006 9274 1632 Size: 482 253 3257 1998 1384 268 1917 Fragments arranged by size: 3257 1998 1917 1384 482 268 253 AfeI AGC'GCT Cuts at: 4931 4931 Size: 9559 AflII C'TTAA_G Cuts at: 820 1017 2356 820 Size: 197 1339 8023 Fragments arranged by size: 8023 1339 197 AflIII A'CryG_T Cuts at: 1203 6423 6632 8458 9435 1203 Size: 5220 209 1826 977 1327 Fragments arranged by size: 5220 1826 1327 977 209 AgeI A'CCGG_T Cuts at: 5017 8390 5017 Size: 3373 6186 AhdI GACnn_n'nnGTC Cuts at: 4078 4636 4948 4973 8288 4078 Size: 558 312 25 3315 5349 Fragments arranged by size: 5349 3315 558 312 25 AleI CACnn'nnGTG Cuts at: 1166 2780 1166 Size: 1614 7945 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9559 AlwNI CAG_nnn'CTG Cuts at: 2020 2438 2548 4889 5725 6118 7302 8874 2020 Size: 418 110 2341 836 393 1184 1572 2705 Fragments arranged by size: 2705 2341 1572 1184 836 418 393 110 ApaLI G'TGCA_C Cuts at: 7616 8772 7616 Size: 1156 8403 ApoI r'AATT_y Cuts at: 1493 2792 2830 4051 5226 5647 6868 7094 7246 Size: 1299 38 1221 1175 421 1221 226 152 Cuts at: 7246 9355 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1299 1221 1221 1175 421 226 152 38 AseI AT'TA_AT Cuts at: 160 1032 8113 160 Size: 872 7081 1606 Fragments arranged by size: 7081 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9559 AvaI C'yCGr_G Cuts at: 1870 1943 4247 4337 4762 5402 5780 5960 6884 Size: 73 2304 90 425 640 378 180 924 Cuts at: 6884 1870 Size: 4545 Fragments arranged by size: 4545 2304 924 640 425 378 180 90 73 BamHI G'GATC_C Cuts at: 5556 6588 6889 7256 5556 Size: 1032 301 367 7859 Fragments arranged by size: 7859 1032 367 301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2228 2406 3761 3929 3947 Size: 325 217 627 441 178 1355 168 18 Cuts at: 3947 5825 6181 6880 8335 618 Size: 1878 356 699 1455 1842 Fragments arranged by size: 1878 1842 1455 1355 699 627 441 356 325 217 178 168 18 BanII G_rGCy'C Cuts at: 1391 4194 4574 4709 4761 4785 5353 5574 5688 Size: 2803 380 135 52 24 568 221 114 Cuts at: 5688 5778 6112 6642 6878 1391 Size: 90 334 530 236 4072 Fragments arranged by size: 4072 2803 568 530 380 334 236 221 135 114 90 52 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2637 4632 4635 928 Size: 971 738 1995 3 5852 Fragments arranged by size: 5852 1995 971 738 3 BbvCI CC'TCA_GC Cuts at: 2859 3080 2859 Size: 221 9338 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6837 6871 7750 7784 1903 Size: 34 4900 34 879 34 3678 Fragments arranged by size: 4900 3678 879 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5244 7451 8661 5244 Size: 2207 1210 6142 Fragments arranged by size: 6142 2207 1210 BclI T'GATC_A Cuts at: 1548 5220 6501 1548 Size: 3672 1281 4606 Fragments arranged by size: 4606 3672 1281 BfrBI ATG'CAT Cuts at: 5140 5140 Size: 9559 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6261 8170 136 Size: 107 122 71 631 605 4589 1909 1525 Fragments arranged by size: 4589 1909 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5745 9554 5745 Size: 3809 5750 BlpI GC'TnA_GC Cuts at: 6111 6976 6111 Size: 865 8694 Bme1580I G_kGCm'C Cuts at: 1790 4175 6651 7620 8776 1790 Size: 2385 2476 969 1156 2573 Fragments arranged by size: 2573 2476 2385 1156 969 BmgBI CAC'GTC Cuts at: 1318 5995 1318 Size: 4677 4882 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2376 3472 4078 Size: 416 53 83 399 24 959 1096 606 Cuts at: 4078 4134 5293 7290 8248 442 Size: 56 1159 1997 958 1753 Fragments arranged by size: 1997 1753 1159 1096 959 958 606 416 399 83 56 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9559 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2635 4210 2635 Size: 1575 7984 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2266 2495 2748 2926 3179 3302 3641 Size: 971 56 229 253 178 253 123 339 Cuts at: 3641 4419 4896 6176 6287 6368 8219 1239 Size: 778 477 1280 111 81 1851 2579 Fragments arranged by size: 2579 1851 1280 971 778 477 339 253 253 229 178 123 111 81 56 Bpu10I CC'TnA_GC Cuts at: 2076 2649 2859 2883 3080 2076 Size: 573 210 24 197 8555 Fragments arranged by size: 8555 573 210 197 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3200 4558 6063 7684 8549 8847 3200 Size: 1358 1505 1621 865 298 3912 Fragments arranged by size: 3912 1621 1505 1358 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2959 3167 3491 3977 4397 4471 8222 Size: 617 1460 208 324 486 420 74 3751 Cuts at: 8222 882 Size: 2219 Fragments arranged by size: 3751 2219 1460 617 486 420 324 208 74 BsaAI yAC'GTr Cuts at: 493 1204 3439 7350 493 Size: 711 2235 3911 2702 Fragments arranged by size: 3911 2702 2235 711 BsaBI GATnn'nnATC Cuts at: 3747 9553 3747 Size: 5806 3753 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4649 6366 7366 7748 Size: 53 83 186 936 3116 1717 1000 382 Cuts at: 7748 8383 275 Size: 635 1451 Fragments arranged by size: 3116 1717 1451 1000 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2142 3555 5017 6042 7992 8390 8664 8811 9222 Size: 1413 1462 1025 1950 398 274 147 411 Cuts at: 9222 2142 Size: 2479 Fragments arranged by size: 2479 1950 1462 1413 1025 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2921 2951 3440 3470 1178 Size: 30 165 30 1518 30 489 30 7267 Fragments arranged by size: 7267 1518 489 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3068 3176 3252 3613 3908 4202 4579 5497 Size: 1882 108 76 361 295 294 377 918 Cuts at: 5497 6300 1186 Size: 803 4445 Fragments arranged by size: 4445 1882 918 803 377 361 295 294 108 76 BseYI C'CCAG_C Cuts at: 2800 3580 3691 3838 4889 5489 6123 6231 6802 Size: 780 111 147 1051 600 634 108 571 Cuts at: 6802 8762 9258 2800 Size: 1960 496 3101 Fragments arranged by size: 3101 1960 1051 780 634 600 571 496 147 111 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 5042 5723 1163 Size: 573 705 2601 681 4999 Fragments arranged by size: 4999 2601 705 681 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3225 4726 5499 7009 7770 8798 Size: 275 186 1224 1501 773 1510 761 1028 Cuts at: 8798 1540 Size: 2301 Fragments arranged by size: 2301 1510 1501 1224 1028 773 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2196 2942 3036 4147 4686 4709 4761 4785 5361 Size: 746 94 1111 539 23 52 24 576 Cuts at: 5361 5574 5778 7620 7705 8776 2196 Size: 213 204 1842 85 1071 2979 Fragments arranged by size: 2979 1842 1111 1071 746 576 539 213 204 94 85 52 24 23 BsmI GAATG_Cn' Cuts at: 7070 7163 7070 Size: 93 9466 BsmBI CGTCTCn'nnnn_ Cuts at: 6001 6022 6833 6838 6001 Size: 21 811 5 8722 Fragments arranged by size: 8722 811 21 5 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2942 3036 4147 4175 4194 4574 Size: 399 406 746 94 1111 28 19 380 Cuts at: 4574 4686 4709 4761 4785 5353 5361 5574 5688 Size: 112 23 52 24 568 8 213 114 Cuts at: 5688 5778 6112 6642 6651 6878 7620 7705 8776 Size: 90 334 530 9 227 742 85 1071 Cuts at: 8776 1391 Size: 2174 Fragments arranged by size: 2174 1111 1071 746 742 568 530 406 399 380 334 227 213 114 112 94 90 85 52 28 24 23 19 9 8 BspEI T'CCGG_A Cuts at: 3555 6042 9222 3555 Size: 2487 3180 3892 Fragments arranged by size: 3892 3180 2487 BspHI T'CATG_A Cuts at: 7448 9178 7448 Size: 1730 7829 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3644 5745 6579 6830 6914 844 Size: 2800 2101 834 251 84 3489 Fragments arranged by size: 3489 2800 2101 834 251 84 BsrBI CCG'CTC Cuts at: 1394 1823 4728 5518 7446 1394 Size: 429 2905 790 1928 3507 Fragments arranged by size: 3507 2905 1928 790 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5484 8054 8228 65 Size: 1183 4236 2570 174 1396 Fragments arranged by size: 4236 2570 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2955 3220 4283 4803 5017 6127 6260 Size: 110 1145 265 1063 520 214 1110 133 Cuts at: 6260 6417 8203 8390 1700 Size: 157 1786 187 2869 Fragments arranged by size: 2869 1786 1145 1110 1063 520 265 214 187 157 133 110 BsrGI T'GTAC_A Cuts at: 96 6579 96 Size: 6483 3076 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9559 BssSI C'ACGA_G Cuts at: 2052 2780 3472 4694 7619 8631 2052 Size: 728 692 1222 2925 1012 2980 Fragments arranged by size: 2980 2925 1222 1012 728 692 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9559 BstBI TT'CG_AA Cuts at: 3676 8363 3676 Size: 4687 4872 BstEII G'GTnAC_C Cuts at: 1184 5095 1184 Size: 3911 5648 BstXI CCAn_nnnn'nTGG Cuts at: 3049 3157 4126 6139 3049 Size: 108 969 2013 6469 Fragments arranged by size: 6469 2013 969 108 BstYI r'GATC_y Cuts at: 1972 3450 5171 5556 5745 6588 6889 7256 7641 Size: 1478 1721 385 189 843 301 367 385 Cuts at: 7641 7658 9110 9196 9208 9554 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1721 1478 1452 843 385 385 367 346 301 189 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2255 2444 3143 3198 3261 3444 Size: 552 939 251 189 699 55 63 183 Cuts at: 3444 3629 5626 5838 6597 513 Size: 185 1997 212 759 3475 Fragments arranged by size: 3475 1997 939 759 699 552 251 212 189 185 183 63 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2622 5908 487 Size: 683 56 1396 3286 4138 Fragments arranged by size: 4138 3286 1396 683 56 BtsI GCAGTG_nn' Cuts at: 3110 4678 5652 7075 7869 7889 3110 Size: 1568 974 1423 794 20 4780 Fragments arranged by size: 4780 1568 1423 974 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9559 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9524 DraI TTT'AAA Cuts at: 4903 6863 4903 Size: 1960 7599 DraIII CAC_nnn'GTG Cuts at: 3547 5301 6613 3547 Size: 1754 1312 6493 Fragments arranged by size: 6493 1754 1312 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5141 5891 8566 809 Size: 507 3825 750 2675 1802 Fragments arranged by size: 3825 2675 1802 750 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3222 3708 5496 Size: 54 1425 50 275 18 1392 486 1788 Cuts at: 5496 6129 6415 6594 7006 7895 9524 8 Size: 633 286 179 412 889 1629 43 Fragments arranged by size: 1788 1629 1425 1392 889 633 486 412 286 275 179 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3222 5496 7006 1537 Size: 275 1410 2274 1510 4090 Fragments arranged by size: 4090 2274 1510 1410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2764 3165 3872 5466 6095 6671 7489 1353 Size: 1411 401 707 1594 629 576 818 3423 Fragments arranged by size: 3423 1594 1411 818 707 629 576 401 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5891 6677 8146 8520 8666 387 Size: 1064 138 4302 786 1469 374 146 1280 Fragments arranged by size: 4302 1469 1280 1064 786 374 146 138 EcoICRI GAG'CTC Cuts at: 4707 4759 4783 5572 5776 4707 Size: 52 24 789 204 8490 Fragments arranged by size: 8490 789 204 52 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2210 2266 2367 2495 2748 2926 Size: 393 482 96 56 101 128 253 178 Cuts at: 2926 3179 3302 3641 4419 4896 5624 6176 6287 Size: 253 123 339 778 477 728 552 111 Cuts at: 6287 6368 7622 8219 9006 9274 1239 Size: 81 1254 597 787 268 1524 Fragments arranged by size: 1524 1254 787 778 728 597 552 482 477 393 339 268 253 253 178 128 123 111 101 96 81 56 EcoO109I rG'GnC_Cy Cuts at: 2170 2659 3835 4394 5124 2170 Size: 489 1176 559 730 6605 Fragments arranged by size: 6605 1176 730 559 489 EcoRI G'AATT_C Cuts at: 4051 6868 7246 4051 Size: 2817 378 6364 Fragments arranged by size: 6364 2817 378 EcoRV GAT'ATC Cuts at: 3553 5056 3553 Size: 1503 8056 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3194 3194 Size: 9559 FspI TGC'GCA Cuts at: 8065 8065 Size: 9559 HaeII r_GCGC'y Cuts at: 1678 2398 3077 4552 4933 5726 6856 8706 1678 Size: 720 679 1475 381 793 1130 1850 2531 Fragments arranged by size: 2531 1850 1475 1130 793 720 679 381 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2231 2263 2624 2656 3196 Size: 32 135 32 659 32 361 32 540 Cuts at: 3196 3228 3365 3397 3440 3472 4064 4096 4622 Size: 32 137 32 43 32 592 32 526 Cuts at: 4622 4654 4856 4888 4934 4959 4966 4991 5358 Size: 32 202 32 46 25 7 25 367 Cuts at: 5358 5390 5561 5593 6279 6311 6463 6495 8200 Size: 32 171 32 686 32 152 32 1705 Cuts at: 8200 8232 8274 8306 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1705 686 659 592 540 526 367 361 202 171 152 137 135 46 43 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 2224 3595 3787 3862 4465 6799 Size: 988 281 286 1371 192 75 603 2334 Cuts at: 6799 6903 7149 669 Size: 104 246 3079 Fragments arranged by size: 3079 2334 1371 988 603 286 281 246 192 104 75 HindIII A'AGCT_T Cuts at: 748 3302 3524 3776 4013 748 Size: 2554 222 252 237 6294 Fragments arranged by size: 6294 2554 252 237 222 HpaI GTT'AAC Cuts at: 7149 7149 Size: 9559 KpnI G_GTAC'C Cuts at: 6884 6884 Size: 9559 MfeI C'AATT_G Cuts at: 2180 7158 2180 Size: 4978 4581 MluI A'CGCG_T Cuts at: 9435 9435 Size: 9559 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3483 4228 4773 6285 8673 8857 1305 Size: 2178 745 545 1512 2388 184 2007 Fragments arranged by size: 2388 2178 2007 1512 745 545 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3710 6596 10 Size: 54 1425 343 1878 2886 2973 Fragments arranged by size: 2973 2886 1878 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2316 2780 3047 3437 4124 4509 5282 Size: 648 1150 464 267 390 687 385 773 Cuts at: 5282 5837 7517 7876 8035 518 Size: 555 1680 359 159 2042 Fragments arranged by size: 2042 1680 1150 773 687 648 555 464 390 385 359 267 159 MspA1I CmG'CkG Cuts at: 1606 2257 3661 3691 3740 3842 3954 3992 5644 Size: 651 1404 30 49 102 112 38 1652 Cuts at: 5644 6115 6187 6231 6975 7652 8800 9045 1606 Size: 471 72 44 744 677 1148 245 2120 Fragments arranged by size: 2120 1652 1404 1148 744 677 651 471 245 112 102 72 49 44 38 30 NaeI GCC'GGC Cuts at: 2957 3222 4805 6129 6419 2957 Size: 265 1583 1324 290 6097 Fragments arranged by size: 6097 1583 1324 290 265 NcoI C'CATG_G Cuts at: 513 1065 2004 3143 3261 3444 5838 6597 513 Size: 552 939 1139 118 183 2394 759 3475 Fragments arranged by size: 3475 2394 1139 939 759 552 183 118 NdeI CA'TA_TG Cuts at: 387 5853 387 Size: 5466 4093 NgoMIV G'CCGG_C Cuts at: 2955 3220 4803 6127 6417 2955 Size: 265 1583 1324 290 6097 Fragments arranged by size: 6097 1583 1324 290 265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9559 NotI GC'GGCC_GC Cuts at: 7006 7006 Size: 9559 NsiI A_TGCA'T Cuts at: 5142 5142 Size: 9559 NspI r_CATG'y Cuts at: 1132 1229 4138 5140 5144 6427 6636 6917 8462 Size: 97 2909 1002 4 1283 209 281 1545 Cuts at: 8462 1132 Size: 2229 Fragments arranged by size: 2909 2229 1545 1283 1002 281 209 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9559 PciI A'CATG_T Cuts at: 6423 6632 8458 6423 Size: 209 1826 7524 Fragments arranged by size: 7524 1826 209 PflMI CCAn_nnn'nTGG Cuts at: 1108 3262 3547 3940 4210 5075 6613 1108 Size: 2154 285 393 270 865 1538 4054 Fragments arranged by size: 4054 2154 1538 865 393 285 270 PfoI T'CCnGG_A Cuts at: 4396 4643 4773 5559 5798 4396 Size: 247 130 786 239 8157 Fragments arranged by size: 8157 786 247 239 130 PmeI GTTT'AAAC Cuts at: 6863 6863 Size: 9559 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 7628 7660 9141 9173 3896 Size: 32 3700 32 1481 32 4282 Fragments arranged by size: 4282 3700 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2170 2659 4394 5124 2170 Size: 489 1735 730 6605 Fragments arranged by size: 6605 1735 730 489 PshAI GACnn'nnGTC Cuts at: 1613 2971 1613 Size: 1358 8201 PsiI TTA'TAA Cuts at: 7129 7129 Size: 9559 PstI C_TGCA'G Cuts at: 830 3069 3117 4791 5466 6911 830 Size: 2239 48 1674 675 1445 3478 Fragments arranged by size: 3478 2239 1674 1445 675 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9559 PvuII CAG'CTG Cuts at: 3661 3691 3740 3842 3992 5644 6115 6231 3661 Size: 30 49 102 150 1652 471 116 6989 Fragments arranged by size: 6989 1652 471 150 116 102 49 30 SacI G_AGCT'C Cuts at: 4709 4761 4785 5574 5778 4709 Size: 52 24 789 204 8490 Fragments arranged by size: 8490 789 204 52 24 SacII CC_GC'GG Cuts at: 2258 2258 Size: 9559 SalI G'TCGA_C Cuts at: 1936 6901 1936 Size: 4965 4594 SapI GCTCTTCn'nnn_ Cuts at: 2764 3872 2764 Size: 1108 8451 SbfI CC_TGCA'GG Cuts at: 5466 6911 5466 Size: 1445 8114 ScaI AGT'ACT Cuts at: 5786 7807 9519 5786 Size: 2021 1712 5826 Fragments arranged by size: 5826 2021 1712 SexAI A'CCwGG_T Cuts at: 4634 6609 4634 Size: 1975 7584 SfcI C'TryA_G Cuts at: 826 1045 3065 3113 4787 5462 6907 8042 8723 Size: 219 2020 48 1674 675 1445 1135 681 Cuts at: 8723 8914 826 Size: 191 1471 Fragments arranged by size: 2020 1674 1471 1445 1135 681 675 219 191 48 SfiI GGCCn_nnn'nGGCC Cuts at: 6261 6261 Size: 9559 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9559 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9559 SmaI CCC'GGG Cuts at: 1872 4249 4764 6886 1872 Size: 2377 515 2122 4545 Fragments arranged by size: 4545 2377 2122 515 SmlI C'TyrA_G Cuts at: 820 1017 1943 2356 3179 4573 5402 6078 7663 Size: 197 926 413 823 1394 829 676 1585 Cuts at: 7663 8564 8826 820 Size: 901 262 1553 Fragments arranged by size: 1585 1553 1394 926 901 829 823 676 413 262 197 SnaBI TAC'GTA Cuts at: 493 7350 493 Size: 6857 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9559 SphI G_CATG'C Cuts at: 1132 6917 1132 Size: 5785 3774 SspI AAT'ATT Cuts at: 5 52 2124 4097 4998 5062 6316 7483 5 Size: 47 2072 1973 901 64 1254 1167 2081 Fragments arranged by size: 2081 2072 1973 1254 1167 901 64 47 StuI AGG'CCT Cuts at: 1517 4425 5330 5931 1517 Size: 2908 905 601 5145 Fragments arranged by size: 5145 2908 905 601 StyI C'CwwG_G Cuts at: 513 1065 2004 3143 3261 3369 3444 4470 5007 Size: 552 939 1139 118 108 75 1026 537 Cuts at: 5007 5325 5838 6597 6998 513 Size: 318 513 759 401 3074 Fragments arranged by size: 3074 1139 1026 939 759 552 537 513 401 318 118 108 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3469 3663 3947 4118 4421 5312 5543 Size: 1255 1889 194 284 171 303 891 231 Cuts at: 5543 7586 7603 7756 7941 8386 325 Size: 2043 17 153 185 445 1498 Fragments arranged by size: 2043 1889 1498 1255 891 445 303 284 231 194 185 171 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5784 6579 7805 Size: 274 80 33 51 1443 3807 795 1226 Cuts at: 7805 9517 96 Size: 1712 138 Fragments arranged by size: 3807 1712 1443 1226 795 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2921 2953 5996 6028 9519 9551 2921 Size: 32 3043 32 3491 32 2929 Fragments arranged by size: 3491 3043 2929 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4379 4651 1316 Size: 3063 272 6224 Fragments arranged by size: 6224 3063 272 XbaI T'CTAG_A Cuts at: 6895 6895 Size: 9559 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3141 3941 4323 5836 3141 Size: 800 382 1513 6864 Fragments arranged by size: 6864 1513 800 382 XhoI C'TCGA_G Cuts at: 1943 5402 1943 Size: 3459 6100 XmaI C'CCGG_G Cuts at: 1870 4247 4762 6884 1870 Size: 2377 515 2122 4545 Fragments arranged by size: 4545 2377 2122 515 XmnI GAAnn'nnTTC Cuts at: 4051 7688 4051 Size: 3637 5922 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6367 7367 276 Size: 53 83 186 936 4833 1000 2468 Fragments arranged by size: 4833 2468 1000 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SfoI SrfI SwaI