(Circular) (Six-base) MAPSORT of: pFN21AA0555.seq Check: 8631 from: 1 to: 7102 pFN21AA0555 7102 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2488 2801 2488 Size: 313 6789 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4912 278 Size: 53 83 186 936 3376 2468 Fragments arranged by size: 3376 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4445 1937 Size: 2508 4594 Acc65I G'GTAC_C Cuts at: 4423 4423 Size: 7102 AclI AA'CG_TT Cuts at: 1563 5229 5602 1563 Size: 3666 373 3063 Fragments arranged by size: 3666 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2705 3137 3170 3438 4235 5165 6549 6817 Size: 1073 432 33 268 797 930 1384 268 Cuts at: 6817 1632 Size: 1917 Fragments arranged by size: 1917 1384 1073 930 797 432 268 268 33 AfeI AGC'GCT Cuts at: 2400 2400 Size: 7102 AflII C'TTAA_G Cuts at: 820 1017 2453 820 Size: 197 1436 5469 Fragments arranged by size: 5469 1436 197 AflIII A'CryG_T Cuts at: 1203 2947 6001 6978 1203 Size: 1744 3054 977 1327 Fragments arranged by size: 3054 1744 1327 977 AgeI A'CCGG_T Cuts at: 3645 5933 3645 Size: 2288 4814 AhdI GACnn_n'nnGTC Cuts at: 5831 5831 Size: 7102 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7102 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7102 AlwNI CAG_nnn'CTG Cuts at: 4845 6417 4845 Size: 1572 5530 ApaLI G'TGCA_C Cuts at: 5159 6315 5159 Size: 1156 5946 ApoI r'AATT_y Cuts at: 1493 2796 3076 3753 3871 4290 4411 4637 4789 Size: 1303 280 677 118 419 121 226 152 Cuts at: 4789 6898 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1303 677 419 280 226 152 121 118 AseI AT'TA_AT Cuts at: 160 1032 5656 160 Size: 872 4624 1606 Fragments arranged by size: 4624 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7102 AvaI C'yCGr_G Cuts at: 1870 1943 2039 2415 4427 1870 Size: 73 96 376 2012 4545 Fragments arranged by size: 4545 2012 376 96 73 BamHI G'GATC_C Cuts at: 3939 4432 4799 3939 Size: 493 367 6242 Fragments arranged by size: 6242 493 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4423 5878 618 Size: 325 217 627 2636 1455 1842 Fragments arranged by size: 2636 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3222 4269 4421 1391 Size: 1831 1047 152 4072 Fragments arranged by size: 4072 1831 1047 152 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2082 2162 2907 3591 928 Size: 971 183 80 745 684 4439 Fragments arranged by size: 4439 971 745 684 183 80 BbvCI CC'TCA_GC Cuts at: 3960 4181 4217 3960 Size: 221 36 6845 Fragments arranged by size: 6845 221 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5293 5327 1903 Size: 34 3356 34 3678 Fragments arranged by size: 3678 3356 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4994 6204 4994 Size: 1210 5892 BclI T'GATC_A Cuts at: 1548 1548 Size: 7102 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5713 136 Size: 107 122 71 631 605 4041 1525 Fragments arranged by size: 4041 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3789 7097 3789 Size: 3308 3794 BlpI GC'TnA_GC Cuts at: 4519 4519 Size: 7102 Bme1580I G_kGCm'C Cuts at: 1790 5163 6319 1790 Size: 3373 1156 2573 Fragments arranged by size: 3373 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7102 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4833 5791 442 Size: 416 53 83 399 24 3416 958 1753 Fragments arranged by size: 3416 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7102 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3257 3257 Size: 7102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2705 3713 4055 5762 1239 Size: 968 498 1008 342 1707 2579 Fragments arranged by size: 2579 1707 1008 968 498 342 Bpu10I CC'TnA_GC Cuts at: 3563 3960 4181 4217 3563 Size: 397 221 36 6448 Fragments arranged by size: 6448 397 221 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2145 2330 3522 5227 6092 6390 2145 Size: 185 1192 1705 865 298 2857 Fragments arranged by size: 2857 1705 1192 865 298 185 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2681 3669 5765 882 Size: 617 1182 988 2096 2219 Fragments arranged by size: 2219 2096 1182 988 617 BsaAI yAC'GTr Cuts at: 493 1204 2324 4893 493 Size: 711 1120 2569 2702 Fragments arranged by size: 2702 2569 1120 711 BsaBI GATnn'nnATC Cuts at: 7096 7096 Size: 7102 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3669 4909 5291 5926 Size: 53 83 186 936 2136 1240 382 635 Cuts at: 5926 275 Size: 1451 Fragments arranged by size: 2136 1451 1240 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3645 5535 5933 6207 6354 6765 3645 Size: 1890 398 274 147 411 3982 Fragments arranged by size: 3982 1890 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2697 2727 2753 2783 1178 Size: 30 165 30 1294 30 26 30 5497 Fragments arranged by size: 5497 1294 165 30 30 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2435 4190 1186 Size: 1069 180 1755 4098 Fragments arranged by size: 4098 1755 1069 180 BseYI C'CCAG_C Cuts at: 2546 6305 6801 2546 Size: 3759 496 2847 Fragments arranged by size: 3759 2847 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6529 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4552 5313 6341 1540 Size: 275 186 2551 761 1028 2301 Fragments arranged by size: 2551 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2365 3222 3784 4269 5163 5248 6319 2365 Size: 857 562 485 894 85 1071 3148 Fragments arranged by size: 3148 1071 894 857 562 485 85 BsmI GAATG_Cn' Cuts at: 2884 4613 4706 2884 Size: 1729 93 5280 Fragments arranged by size: 5280 1729 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3721 3721 Size: 7102 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2365 3222 3784 4269 4421 5163 5248 Size: 399 575 857 562 485 152 742 85 Cuts at: 5248 6319 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 857 742 575 562 485 399 152 85 BspEI T'CCGG_A Cuts at: 6765 6765 Size: 7102 BspHI T'CATG_A Cuts at: 4991 6721 4991 Size: 1730 5372 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2488 2801 4457 844 Size: 1644 313 1656 3489 Fragments arranged by size: 3489 1656 1644 313 BsrBI CCG'CTC Cuts at: 1394 1823 2614 4989 1394 Size: 429 791 2375 3507 Fragments arranged by size: 3507 2375 791 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2975 5597 5771 65 Size: 1183 1727 2622 174 1396 Fragments arranged by size: 2622 1727 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3645 5746 5933 1700 Size: 110 1835 2101 187 2869 Fragments arranged by size: 2869 2101 1835 187 110 BsrGI T'GTAC_A Cuts at: 96 2704 4110 96 Size: 2608 1406 3088 Fragments arranged by size: 3088 2608 1406 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7102 BssSI C'ACGA_G Cuts at: 2291 5162 6174 2291 Size: 2871 1012 3219 Fragments arranged by size: 3219 2871 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2206 794 Size: 1412 5690 BstBI TT'CG_AA Cuts at: 3700 5906 3700 Size: 2206 4896 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7102 BstYI r'GATC_y Cuts at: 1972 2334 3789 3939 4432 4799 5184 5201 6653 Size: 362 1455 150 493 367 385 17 1452 Cuts at: 6653 6739 6751 7097 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1455 1452 493 385 367 362 346 150 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2081 2081 Size: 7102 BtgI C'CryG_G Cuts at: 513 1065 3348 513 Size: 552 2283 4267 Fragments arranged by size: 4267 2283 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6363 Fragments arranged by size: 6363 683 56 BtsI GCAGTG_nn' Cuts at: 2386 2827 4618 5412 5432 2386 Size: 441 1791 794 20 4056 Fragments arranged by size: 4056 1791 794 441 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7102 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7067 DraI TTT'AAA Cuts at: 4406 4406 Size: 7102 DraIII CAC_nnn'GTG Cuts at: 2206 2206 Size: 7102 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6109 809 Size: 507 4793 1802 Fragments arranged by size: 4793 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2487 3894 4549 Size: 54 1425 50 275 18 657 1407 655 Cuts at: 4549 5438 7067 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1407 889 657 655 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4549 1537 Size: 275 2737 4090 Fragments arranged by size: 4090 2737 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 3711 4217 5032 1353 Size: 812 1546 506 815 3423 Fragments arranged by size: 3423 1546 815 812 506 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5689 6063 6209 387 Size: 1064 138 4100 374 146 1280 Fragments arranged by size: 4100 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3220 4267 3220 Size: 1047 6055 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2705 3137 3170 3438 3713 4055 Size: 393 575 498 432 33 268 275 342 Cuts at: 4055 4235 5165 5762 6549 6817 1239 Size: 180 930 597 787 268 1524 Fragments arranged by size: 1524 930 787 597 575 498 432 393 342 275 268 268 180 33 EcoNI CCTnn'n_nnAGG Cuts at: 2675 3488 2675 Size: 813 6289 EcoO109I rG'GnC_Cy Cuts at: 2551 3463 2551 Size: 912 6190 EcoRI G'AATT_C Cuts at: 4290 4411 4789 4290 Size: 121 378 6603 Fragments arranged by size: 6603 378 121 EcoRV GAT'ATC Cuts at: 3853 3853 Size: 7102 FspI TGC'GCA Cuts at: 5608 5608 Size: 7102 HaeII r_GCGC'y Cuts at: 1678 2402 2452 6249 1678 Size: 724 50 3797 2531 Fragments arranged by size: 3797 2531 724 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2551 2555 2583 2587 3308 Size: 32 135 32 979 4 28 4 721 Cuts at: 3308 3329 3340 3361 3495 3527 3589 3621 3716 Size: 21 11 21 134 32 62 32 95 Cuts at: 3716 3748 5743 5775 5817 5849 1373 Size: 32 1995 32 42 32 2626 Fragments arranged by size: 2626 1995 979 721 135 134 95 62 42 32 32 32 32 32 32 32 28 21 21 11 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 4446 4692 669 Size: 988 281 2508 246 3079 Fragments arranged by size: 3079 2508 988 281 246 HindIII A'AGCT_T Cuts at: 748 2141 748 Size: 1393 5709 HpaI GTT'AAC Cuts at: 4692 4692 Size: 7102 KpnI G_GTAC'C Cuts at: 4427 4427 Size: 7102 MfeI C'AATT_G Cuts at: 4701 4701 Size: 7102 MluI A'CGCG_T Cuts at: 6978 6978 Size: 7102 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2589 2740 6216 6400 1305 Size: 1284 151 3476 184 2007 Fragments arranged by size: 3476 2007 1284 184 151 MscI TGG'CCA Cuts at: 10 64 1489 1832 3896 10 Size: 54 1425 343 2064 3216 Fragments arranged by size: 3216 2064 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5060 5419 5578 518 Size: 648 3894 359 159 2042 Fragments arranged by size: 3894 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3416 4518 5195 6343 6588 1606 Size: 1810 1102 677 1148 245 2120 Fragments arranged by size: 2120 1810 1148 1102 677 245 NcoI C'CATG_G Cuts at: 513 1065 3348 513 Size: 552 2283 4267 Fragments arranged by size: 4267 2283 552 NdeI CA'TA_TG Cuts at: 387 3478 387 Size: 3091 4011 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7102 NotI GC'GGCC_GC Cuts at: 4549 4549 Size: 7102 NspI r_CATG'y Cuts at: 1132 1229 4460 6005 1132 Size: 97 3231 1545 2229 Fragments arranged by size: 3231 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7102 PciI A'CATG_T Cuts at: 6001 6001 Size: 7102 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7102 PfoI T'CCnGG_A Cuts at: 2800 2982 4089 2800 Size: 182 1107 5813 Fragments arranged by size: 5813 1107 182 PmeI GTTT'AAAC Cuts at: 4406 4406 Size: 7102 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5171 5203 6684 6716 5171 Size: 32 1481 32 5557 Fragments arranged by size: 5557 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2551 2551 Size: 7102 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7102 PsiI TTA'TAA Cuts at: 4672 4672 Size: 7102 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3364 3396 3364 Size: 32 7070 PstI C_TGCA'G Cuts at: 830 2262 2834 4454 830 Size: 1432 572 1620 3478 Fragments arranged by size: 3478 1620 1432 572 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7102 PvuII CAG'CTG Cuts at: 3416 3416 Size: 7102 SacI G_AGCT'C Cuts at: 3222 4269 3222 Size: 1047 6055 SalI G'TCGA_C Cuts at: 1936 4444 1936 Size: 2508 4594 SapI GCTCTTCn'nnn_ Cuts at: 4217 4217 Size: 7102 SbfI CC_TGCA'GG Cuts at: 4454 4454 Size: 7102 ScaI AGT'ACT Cuts at: 5350 7062 5350 Size: 1712 5390 SfcI C'TryA_G Cuts at: 826 1045 2258 2830 3488 3662 3785 4051 4121 Size: 219 1213 572 658 174 123 266 70 Cuts at: 4121 4450 5585 6266 6457 826 Size: 329 1135 681 191 1471 Fragments arranged by size: 1471 1213 1135 681 658 572 329 266 219 191 174 123 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7102 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7102 SmaI CCC'GGG Cuts at: 1872 2417 4429 1872 Size: 545 2012 4545 Fragments arranged by size: 4545 2012 545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2160 2345 2453 3537 5206 6107 Size: 197 926 217 185 108 1084 1669 901 Cuts at: 6107 6369 820 Size: 262 1553 Fragments arranged by size: 1669 1553 1084 926 901 262 217 197 185 108 SnaBI TAC'GTA Cuts at: 493 4893 493 Size: 4400 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7102 SphI G_CATG'C Cuts at: 1132 4460 1132 Size: 3328 3774 SspI AAT'ATT Cuts at: 5 52 5026 5 Size: 47 4974 2081 Fragments arranged by size: 4974 2081 47 StuI AGG'CCT Cuts at: 1517 3289 1517 Size: 1772 5330 StyI C'CwwG_G Cuts at: 513 1065 2130 2649 3069 3348 3531 4541 513 Size: 552 1065 519 420 279 183 1010 3074 Fragments arranged by size: 3074 1065 1010 552 519 420 279 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3246 3774 3848 5129 5146 5299 5484 Size: 1255 1666 528 74 1281 17 153 185 Cuts at: 5484 5929 325 Size: 445 1498 Fragments arranged by size: 1666 1498 1281 1255 528 445 185 153 74 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2704 4110 5348 Size: 274 80 33 51 1443 727 1406 1238 Cuts at: 5348 7060 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1406 1238 727 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2753 2785 7062 7094 2753 Size: 32 4277 32 2761 Fragments arranged by size: 4277 2761 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7102 XbaI T'CTAG_A Cuts at: 4438 4438 Size: 7102 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2497 2497 Size: 7102 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7102 XmaI C'CCGG_G Cuts at: 1870 2415 4427 1870 Size: 545 2012 4545 Fragments arranged by size: 4545 2012 545 XmnI GAAnn'nnTTC Cuts at: 3268 4353 5231 3268 Size: 1085 878 5139 Fragments arranged by size: 5139 1085 878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4910 276 Size: 53 83 186 936 3376 2468 Fragments arranged by size: 3376 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI