(Circular) (Six-base) MAPSORT of: pFN21AA0531.seq Check: 6170 from: 1 to: 7576 pFN21AA0531 7576 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5386 278 Size: 53 83 186 936 3850 2468 Fragments arranged by size: 3850 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4919 1937 Size: 2982 4594 Acc65I G'GTAC_C Cuts at: 4897 4897 Size: 7576 AclI AA'CG_TT Cuts at: 1563 5703 6076 1563 Size: 4140 373 3063 Fragments arranged by size: 4140 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2852 3359 4113 4673 5639 7023 7291 1632 Size: 1220 507 754 560 966 1384 268 1917 Fragments arranged by size: 1917 1384 1220 966 754 560 507 268 AfeI AGC'GCT Cuts at: 4691 4691 Size: 7576 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7379 AflIII A'CryG_T Cuts at: 1203 2578 2830 3416 6475 7452 1203 Size: 1375 252 586 3059 977 1327 Fragments arranged by size: 3059 1375 1327 977 586 252 AgeI A'CCGG_T Cuts at: 6407 6407 Size: 7576 AhdI GACnn_n'nnGTC Cuts at: 6305 6305 Size: 7576 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7576 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7576 AlwNI CAG_nnn'CTG Cuts at: 2999 3091 3475 4228 4396 4429 5319 6891 2999 Size: 92 384 753 168 33 890 1572 3684 Fragments arranged by size: 3684 1572 890 753 384 168 92 33 ApaI G_GGCC'C Cuts at: 4756 4756 Size: 7576 ApaLI G'TGCA_C Cuts at: 2509 5633 6789 2509 Size: 3124 1156 3296 Fragments arranged by size: 3296 3124 1156 ApoI r'AATT_y Cuts at: 1493 2087 2099 3658 4503 4855 4885 5111 5263 Size: 594 12 1559 845 352 30 226 152 Cuts at: 5263 7372 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1559 845 594 352 226 152 30 12 AseI AT'TA_AT Cuts at: 160 1032 6130 160 Size: 872 5098 1606 Fragments arranged by size: 5098 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7576 AvaI C'yCGr_G Cuts at: 1870 1943 3531 3827 4437 4901 1870 Size: 73 1588 296 610 464 4545 Fragments arranged by size: 4545 1588 610 464 296 73 BamHI G'GATC_C Cuts at: 2011 4906 5273 2011 Size: 2895 367 4314 Fragments arranged by size: 4314 2895 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2732 3701 4897 6352 618 Size: 325 217 627 945 969 1196 1455 1842 Fragments arranged by size: 1842 1455 1196 969 945 627 325 217 BanII G_rGCy'C Cuts at: 1391 2534 4024 4756 4895 1391 Size: 1143 1490 732 139 4072 Fragments arranged by size: 4072 1490 1143 732 139 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2140 2920 4356 928 Size: 971 241 780 1436 4148 Fragments arranged by size: 4148 1436 971 780 241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3808 3842 4388 4422 5767 5801 1903 Size: 34 1871 34 546 34 1345 34 3678 Fragments arranged by size: 3678 1871 1345 546 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2461 5468 6678 2461 Size: 3007 1210 3359 Fragments arranged by size: 3359 3007 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7576 BfrBI ATG'CAT Cuts at: 2198 2198 Size: 7576 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4730 4751 6187 Size: 107 122 71 631 605 3058 21 1436 Cuts at: 6187 136 Size: 1525 Fragments arranged by size: 3058 1525 1436 631 605 122 107 71 21 BglII A'GATC_T Cuts at: 2415 3264 3673 7571 2415 Size: 849 409 3898 2420 Fragments arranged by size: 3898 2420 849 409 BlpI GC'TnA_GC Cuts at: 3602 3620 3995 4993 3602 Size: 18 375 998 6185 Fragments arranged by size: 6185 998 375 18 Bme1580I G_kGCm'C Cuts at: 1790 2513 4756 5637 6793 1790 Size: 723 2243 881 1156 2573 Fragments arranged by size: 2573 2243 1156 881 723 BmgBI CAC'GTC Cuts at: 1318 3419 1318 Size: 2101 5475 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5307 6265 442 Size: 416 53 83 399 24 3890 958 1753 Fragments arranged by size: 3890 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7576 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3283 4370 3283 Size: 1087 6489 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 3113 3194 3253 3407 3452 3497 4133 Size: 1166 708 81 59 154 45 45 636 Cuts at: 4133 4385 4547 4619 4661 6236 1239 Size: 252 162 72 42 1575 2579 Fragments arranged by size: 2579 1575 1166 708 636 252 162 154 81 72 59 45 45 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5701 6566 6864 5701 Size: 865 298 6413 Fragments arranged by size: 6413 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2108 2936 6239 882 Size: 617 609 828 3303 2219 Fragments arranged by size: 3303 2219 828 617 609 BsaAI yAC'GTr Cuts at: 493 1204 2581 5367 493 Size: 711 1377 2786 2702 Fragments arranged by size: 2786 2702 1377 711 BsaBI GATnn'nnATC Cuts at: 4218 7570 4218 Size: 3352 4224 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5383 5765 6400 275 Size: 53 83 186 936 3850 382 635 1451 Fragments arranged by size: 3850 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6009 6407 6681 6828 7239 6009 Size: 398 274 147 411 6346 Fragments arranged by size: 6346 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3114 3144 4361 4391 1178 Size: 30 165 30 1711 30 1217 30 4363 Fragments arranged by size: 4363 1711 1217 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3254 3275 3422 3494 3761 3764 3979 4196 Size: 2068 21 147 72 267 3 215 217 Cuts at: 4196 4382 4854 1186 Size: 186 472 3908 Fragments arranged by size: 3908 2068 472 267 217 215 186 147 72 21 3 BseYI C'CCAG_C Cuts at: 2313 2705 3906 6779 7275 2313 Size: 392 1201 2873 496 2614 Fragments arranged by size: 2873 2614 1201 496 392 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4382 1163 Size: 573 2646 4357 Fragments arranged by size: 4357 2646 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4818 5026 5787 6815 1540 Size: 275 186 2817 208 761 1028 2301 Fragments arranged by size: 2817 2301 1028 761 275 208 186 BsiHKAI G_wGCw'C Cuts at: 2513 5637 5722 6793 2513 Size: 3124 85 1071 3296 Fragments arranged by size: 3296 3124 1071 85 BsmI GAATG_Cn' Cuts at: 2027 3868 5087 5180 2027 Size: 1841 1219 93 4423 Fragments arranged by size: 4423 1841 1219 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3425 4164 3425 Size: 739 6837 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2513 2534 4024 4756 4895 5637 5722 Size: 399 723 21 1490 732 139 742 85 Cuts at: 5722 6793 1391 Size: 1071 2174 Fragments arranged by size: 2174 1490 1071 742 732 723 399 139 85 21 BspEI T'CCGG_A Cuts at: 7239 7239 Size: 7576 BspHI T'CATG_A Cuts at: 2473 5465 7195 2473 Size: 2992 1730 2854 Fragments arranged by size: 2992 2854 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2176 4584 4931 844 Size: 1332 2408 347 3489 Fragments arranged by size: 3489 2408 1332 347 BsrBI CCG'CTC Cuts at: 1394 1823 2518 3874 5463 1394 Size: 429 695 1356 1589 3507 Fragments arranged by size: 3507 1589 1356 695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6071 6245 65 Size: 1183 4823 174 1396 Fragments arranged by size: 4823 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2043 6220 6407 1700 Size: 110 233 4177 187 2869 Fragments arranged by size: 4177 2869 233 187 110 BsrGI T'GTAC_A Cuts at: 96 3771 96 Size: 3675 3901 BssHII G'CGCG_C Cuts at: 1925 4647 1925 Size: 2722 4854 BssSI C'ACGA_G Cuts at: 5636 6648 5636 Size: 1012 6564 BstAPI GCAn_nnn'nTGC Cuts at: 794 4396 794 Size: 3602 3974 BstBI TT'CG_AA Cuts at: 6380 6380 Size: 7576 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7576 BstXI CCAn_nnnn'nTGG Cuts at: 4741 4741 Size: 7576 BstYI r'GATC_y Cuts at: 1972 2011 2070 2415 3264 3651 3673 4426 4906 Size: 39 59 345 849 387 22 753 480 Cuts at: 4906 5273 5658 5675 7127 7213 7225 7571 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 849 753 480 387 385 367 346 345 86 59 39 22 17 12 Bsu36I CC'TnA_GG Cuts at: 2535 3135 2535 Size: 600 6976 BtgI C'CryG_G Cuts at: 513 1065 2004 2077 2118 2289 2370 3759 4617 Size: 552 939 73 41 171 81 1389 858 Cuts at: 4617 513 Size: 3472 Fragments arranged by size: 3472 1389 939 858 552 171 81 73 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2124 487 Size: 683 56 898 5939 Fragments arranged by size: 5939 898 683 56 BtsI GCAGTG_nn' Cuts at: 4394 4489 5092 5886 5906 4394 Size: 95 603 794 20 6064 Fragments arranged by size: 6064 794 603 95 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7576 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7541 DraI TTT'AAA Cuts at: 2104 4880 2104 Size: 2776 4800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6583 809 Size: 507 5267 1802 Fragments arranged by size: 5267 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4743 4815 5023 Size: 54 1425 50 275 18 2913 72 208 Cuts at: 5023 5912 7541 8 Size: 889 1629 43 Fragments arranged by size: 2913 1629 1425 889 275 208 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4815 5023 1537 Size: 275 3003 208 4090 Fragments arranged by size: 4090 3003 275 208 EarI CTCTTCn'nnn_ Cuts at: 1353 3379 3956 3972 3996 4855 5506 1353 Size: 2026 577 16 24 859 651 3423 Fragments arranged by size: 3423 2026 859 651 577 24 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4637 4772 6163 6537 6683 Size: 1064 138 435 2613 135 1391 374 146 Cuts at: 6683 387 Size: 1280 Fragments arranged by size: 2613 1391 1280 1064 435 374 146 138 135 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2405 2852 3113 3194 3253 3359 3407 Size: 393 773 447 261 81 59 106 48 Cuts at: 3407 3452 3497 4113 4133 4385 4547 4619 4661 Size: 45 45 616 20 252 162 72 42 Cuts at: 4661 4673 5639 6236 7023 7291 1239 Size: 12 966 597 787 268 1524 Fragments arranged by size: 1524 966 787 773 616 597 447 393 268 261 252 162 106 81 72 59 48 45 45 42 20 12 EcoO109I rG'GnC_Cy Cuts at: 3472 4752 3472 Size: 1280 6296 EcoRI G'AATT_C Cuts at: 4885 5263 4885 Size: 378 7198 EcoRV GAT'ATC Cuts at: 2350 2350 Size: 7576 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2243 2479 2243 Size: 236 7340 FspI TGC'GCA Cuts at: 6082 6082 Size: 7576 HaeII r_GCGC'y Cuts at: 1678 3837 4112 4693 6723 1678 Size: 2159 275 581 2030 2531 Fragments arranged by size: 2531 2159 2030 581 275 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2933 2965 3256 3288 3340 Size: 32 135 32 1361 32 291 32 52 Cuts at: 3340 3372 3491 3523 3785 3817 3913 3945 4361 Size: 32 119 32 262 32 96 32 416 Cuts at: 4361 4393 6217 6249 6291 6323 1373 Size: 32 1824 32 42 32 2626 Fragments arranged by size: 2626 1824 1361 416 291 262 135 119 96 52 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4920 5166 669 Size: 988 281 2982 246 3079 Fragments arranged by size: 3079 2982 988 281 246 HindIII A'AGCT_T Cuts at: 748 4247 748 Size: 3499 4077 HpaI GTT'AAC Cuts at: 5166 5166 Size: 7576 KpnI G_GTAC'C Cuts at: 4901 4901 Size: 7576 MfeI C'AATT_G Cuts at: 5175 5175 Size: 7576 MluI A'CGCG_T Cuts at: 7452 7452 Size: 7576 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3333 3873 4440 4835 6690 6874 1305 Size: 2028 540 567 395 1855 184 2007 Fragments arranged by size: 2028 2007 1855 567 540 395 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4745 10 Size: 54 1425 343 2913 2841 Fragments arranged by size: 2913 2841 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 2957 5534 5893 6052 518 Size: 648 867 924 2577 359 159 2042 Fragments arranged by size: 2577 2042 924 867 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2079 3325 3547 3895 4156 4555 4992 5669 Size: 473 1246 222 348 261 399 437 677 Cuts at: 5669 6817 7062 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1246 1148 677 473 437 399 348 261 245 222 NcoI C'CATG_G Cuts at: 513 1065 2004 2289 2370 3759 513 Size: 552 939 285 81 1389 4330 Fragments arranged by size: 4330 1389 939 552 285 81 NdeI CA'TA_TG Cuts at: 387 2143 387 Size: 1756 5820 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7576 NotI GC'GGCC_GC Cuts at: 5023 5023 Size: 7576 NsiI A_TGCA'T Cuts at: 2200 2200 Size: 7576 NspI r_CATG'y Cuts at: 1132 1229 2202 2834 3946 4934 6479 1132 Size: 97 973 632 1112 988 1545 2229 Fragments arranged by size: 2229 1545 1112 988 973 632 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7576 PciI A'CATG_T Cuts at: 2830 6475 2830 Size: 3645 3931 PflMI CCAn_nnn'nTGG Cuts at: 1108 2290 2320 3091 3475 1108 Size: 1182 30 771 384 5209 Fragments arranged by size: 5209 1182 771 384 30 PfoI T'CCnGG_A Cuts at: 4050 4422 4560 4050 Size: 372 138 7066 Fragments arranged by size: 7066 372 138 PmeI GTTT'AAAC Cuts at: 4880 4880 Size: 7576 PmlI CAC'GTG Cuts at: 2581 2581 Size: 7576 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5645 5677 7158 7190 5645 Size: 32 1481 32 6031 Fragments arranged by size: 6031 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7576 PsiI TTA'TAA Cuts at: 2238 4263 5146 2238 Size: 2025 883 4668 Fragments arranged by size: 4668 2025 883 PspOMI G'GGCC_C Cuts at: 4752 4752 Size: 7576 PstI C_TGCA'G Cuts at: 830 2894 4928 830 Size: 2064 2034 3478 Fragments arranged by size: 3478 2064 2034 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7576 PvuII CAG'CTG Cuts at: 3325 3547 3895 4156 4555 3325 Size: 222 348 261 399 6346 Fragments arranged by size: 6346 399 348 261 222 SacII CC_GC'GG Cuts at: 2080 2080 Size: 7576 SalI G'TCGA_C Cuts at: 1936 4918 1936 Size: 2982 4594 SapI GCTCTTCn'nnn_ Cuts at: 3996 4855 3996 Size: 859 6717 SbfI CC_TGCA'GG Cuts at: 4928 4928 Size: 7576 ScaI AGT'ACT Cuts at: 5824 7536 5824 Size: 1712 5864 SfcI C'TryA_G Cuts at: 826 1045 2890 3234 3277 4924 6059 6740 6931 Size: 219 1845 344 43 1647 1135 681 191 Cuts at: 6931 826 Size: 1471 Fragments arranged by size: 1845 1647 1471 1135 681 344 219 191 43 SfiI GGCCn_nnn'nGGCC Cuts at: 4730 4751 4730 Size: 21 7555 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7576 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7576 SmaI CCC'GGG Cuts at: 1872 3533 4903 1872 Size: 1661 1370 4545 Fragments arranged by size: 4545 1661 1370 SmlI C'TyrA_G Cuts at: 820 1017 1943 3827 5680 6581 6843 820 Size: 197 926 1884 1853 901 262 1553 Fragments arranged by size: 1884 1853 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5367 493 Size: 4874 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7576 SphI G_CATG'C Cuts at: 1132 4934 1132 Size: 3802 3774 SspI AAT'ATT Cuts at: 5 52 2641 3208 5500 5 Size: 47 2589 567 2292 2081 Fragments arranged by size: 2589 2292 2081 567 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7576 StyI C'CwwG_G Cuts at: 513 1065 2004 2289 2370 3156 3444 3759 4662 Size: 552 939 285 81 786 288 315 903 Cuts at: 4662 5015 513 Size: 353 3074 Fragments arranged by size: 3074 939 903 786 552 353 315 288 285 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2870 5603 5620 5773 5958 6403 325 Size: 1255 1290 2733 17 153 185 445 1498 Fragments arranged by size: 2733 1498 1290 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3771 5822 7534 Size: 274 80 33 51 1443 1794 2051 1712 Cuts at: 7534 96 Size: 138 Fragments arranged by size: 2051 1794 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3114 3146 7536 7568 3114 Size: 32 4390 32 3122 Fragments arranged by size: 4390 3122 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2947 4375 1316 Size: 1631 1428 4517 Fragments arranged by size: 4517 1631 1428 XbaI T'CTAG_A Cuts at: 2612 4379 4912 2612 Size: 1767 533 5276 Fragments arranged by size: 5276 1767 533 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2461 2461 Size: 7576 XhoI C'TCGA_G Cuts at: 1943 3827 1943 Size: 1884 5692 XmaI C'CCGG_G Cuts at: 1870 3531 4901 1870 Size: 1661 1370 4545 Fragments arranged by size: 4545 1661 1370 XmnI GAAnn'nnTTC Cuts at: 5705 5705 Size: 7576 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5384 276 Size: 53 83 186 936 3850 2468 Fragments arranged by size: 3850 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfoI SrfI SwaI