(Circular) (Six-base) MAPSORT of: pFN21AA0425.seq Check: 1328 from: 1 to: 8377 pFN21AA0425 8377 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2170 2741 2170 Size: 571 7806 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6187 278 Size: 53 83 186 936 4651 2468 Fragments arranged by size: 4651 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2111 2827 5720 1937 Size: 174 716 2893 4594 Fragments arranged by size: 4594 2893 716 174 Acc65I G'GTAC_C Cuts at: 5698 5698 Size: 8377 AclI AA'CG_TT Cuts at: 1563 6504 6877 1563 Size: 4941 373 3063 Fragments arranged by size: 4941 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2007 2525 2886 3630 4865 5294 5670 6440 Size: 375 518 361 744 1235 429 376 770 Cuts at: 6440 7824 8092 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1235 770 744 518 429 376 375 361 268 AfeI AGC'GCT Cuts at: 5522 5522 Size: 8377 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8180 AflIII A'CryG_T Cuts at: 1203 4653 5097 5597 7276 8253 1203 Size: 3450 444 500 1679 977 1327 Fragments arranged by size: 3450 1679 1327 977 500 444 AgeI A'CCGG_T Cuts at: 7208 7208 Size: 8377 AhdI GACnn_n'nnGTC Cuts at: 4858 7106 4858 Size: 2248 6129 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8377 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8377 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2139 2171 4041 4073 2139 Size: 32 1870 32 6443 Fragments arranged by size: 6443 1870 32 32 AlwNI CAG_nnn'CTG Cuts at: 3272 4444 4984 6120 7692 3272 Size: 1172 540 1136 1572 3957 Fragments arranged by size: 3957 1572 1172 1136 540 ApaLI G'TGCA_C Cuts at: 6434 7590 6434 Size: 1156 7221 ApoI r'AATT_y Cuts at: 1493 2869 2896 3739 4050 4971 5686 5912 6064 Size: 1376 27 843 311 921 715 226 152 Cuts at: 6064 8173 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1376 921 843 715 311 226 152 27 AseI AT'TA_AT Cuts at: 160 1032 6931 160 Size: 872 5899 1606 Fragments arranged by size: 5899 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8377 AvaI C'yCGr_G Cuts at: 1870 1943 5702 1870 Size: 73 3759 4545 Fragments arranged by size: 4545 3759 73 BamHI G'GATC_C Cuts at: 5707 6074 5707 Size: 367 8010 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3976 5698 7153 618 Size: 325 217 627 2189 1722 1455 1842 Fragments arranged by size: 2189 1842 1722 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4593 5025 5696 1391 Size: 3202 432 671 4072 Fragments arranged by size: 4072 3202 671 432 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2693 3951 4134 4220 4857 5658 928 Size: 971 794 1258 183 86 637 801 3647 Fragments arranged by size: 3647 1258 971 801 794 637 183 86 BbvCI CC'TCA_GC Cuts at: 2650 2785 2650 Size: 135 8242 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6568 6602 1903 Size: 34 4631 34 3678 Fragments arranged by size: 4631 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6269 7479 6269 Size: 1210 7167 BclI T'GATC_A Cuts at: 1548 2238 1548 Size: 690 7687 BfrBI ATG'CAT Cuts at: 3438 3438 Size: 8377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6988 136 Size: 107 122 71 631 605 5316 1525 Fragments arranged by size: 5316 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8372 8372 Size: 8377 BlpI GC'TnA_GC Cuts at: 3726 5794 3726 Size: 2068 6309 Bme1580I G_kGCm'C Cuts at: 1790 2248 2913 3718 6438 7594 1790 Size: 458 665 805 2720 1156 2573 Fragments arranged by size: 2720 2573 1156 805 665 458 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8377 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2242 3040 4690 Size: 416 53 83 399 24 825 798 1650 Cuts at: 4690 5432 6108 7066 442 Size: 742 676 958 1753 Fragments arranged by size: 1753 1650 958 825 798 742 676 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 3014 3666 4020 7037 1239 Size: 821 954 652 354 3017 2579 Fragments arranged by size: 3017 2579 954 821 652 354 Bpu10I CC'TnA_GC Cuts at: 2650 2785 5517 2650 Size: 135 2732 5510 Fragments arranged by size: 5510 2732 135 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4196 4767 4785 6502 7367 7665 4196 Size: 571 18 1717 865 298 4908 Fragments arranged by size: 4908 1717 865 571 298 18 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3032 4912 7040 882 Size: 617 1533 1880 2128 2219 Fragments arranged by size: 2219 2128 1880 1533 617 BsaAI yAC'GTr Cuts at: 493 1204 5600 6168 493 Size: 711 4396 568 2702 Fragments arranged by size: 4396 2702 711 568 BsaBI GATnn'nnATC Cuts at: 8371 8371 Size: 8377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6184 6566 7201 275 Size: 53 83 186 936 4651 382 635 1451 Fragments arranged by size: 4651 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6810 7208 7482 7629 8040 6810 Size: 398 274 147 411 7147 Fragments arranged by size: 7147 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3396 3426 1178 Size: 30 165 30 1993 30 6129 Fragments arranged by size: 6129 1993 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2169 2985 1186 Size: 983 816 6578 Fragments arranged by size: 6578 983 816 BseYI C'CCAG_C Cuts at: 3051 3333 4344 5143 7580 8076 3051 Size: 282 1011 799 2437 496 3352 Fragments arranged by size: 3352 2437 1011 799 496 282 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3376 3783 4849 1163 Size: 573 1640 407 1066 4691 Fragments arranged by size: 4691 1640 1066 573 407 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5827 6588 7616 1540 Size: 275 186 3826 761 1028 2301 Fragments arranged by size: 3826 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5229 6438 6523 7594 5229 Size: 1209 85 1071 6012 Fragments arranged by size: 6012 1209 1071 85 BsmI GAATG_Cn' Cuts at: 2386 3440 3938 5888 5981 2386 Size: 1054 498 1950 93 4782 Fragments arranged by size: 4782 1950 1054 498 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3041 5251 3041 Size: 2210 6167 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2248 2913 3718 4593 5025 5229 5696 Size: 399 458 665 805 875 432 204 467 Cuts at: 5696 6438 6523 7594 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 875 805 742 665 467 458 432 399 204 85 BspEI T'CCGG_A Cuts at: 8040 8040 Size: 8377 BspHI T'CATG_A Cuts at: 6266 7996 6266 Size: 1730 6647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2170 2741 3561 5732 844 Size: 1326 571 820 2171 3489 Fragments arranged by size: 3489 2171 1326 820 571 BsrBI CCG'CTC Cuts at: 1394 1823 4833 6264 1394 Size: 429 3010 1431 3507 Fragments arranged by size: 3507 3010 1431 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3219 6872 7046 65 Size: 1183 1971 3653 174 1396 Fragments arranged by size: 3653 1971 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7021 7208 1700 Size: 110 5211 187 2869 Fragments arranged by size: 5211 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2755 96 Size: 2659 5718 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8377 BssSI C'ACGA_G Cuts at: 6437 7449 6437 Size: 1012 7365 BstAPI GCAn_nnn'nTGC Cuts at: 794 2838 794 Size: 2044 6333 BstBI TT'CG_AA Cuts at: 7181 7181 Size: 8377 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8377 BstXI CCAn_nnnn'nTGG Cuts at: 2680 3059 4344 2680 Size: 379 1285 6713 Fragments arranged by size: 6713 1285 379 BstYI r'GATC_y Cuts at: 1972 2645 4717 5707 6074 6459 6476 7928 8014 Size: 673 2072 990 367 385 17 1452 86 Cuts at: 8014 8026 8372 1972 Size: 12 346 1977 Fragments arranged by size: 2072 1977 1452 990 673 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2828 2828 Size: 8377 Bsu36I CC'TnA_GG Cuts at: 4771 4771 Size: 8377 BtgI C'CryG_G Cuts at: 513 1065 3630 513 Size: 552 2565 5260 Fragments arranged by size: 5260 2565 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7638 Fragments arranged by size: 7638 683 56 BtsI GCAGTG_nn' Cuts at: 2440 5893 6687 6707 2440 Size: 3453 794 20 4110 Fragments arranged by size: 4110 3453 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8342 DraI TTT'AAA Cuts at: 2223 5172 5681 2223 Size: 2949 509 4919 Fragments arranged by size: 4919 2949 509 DraIII CAC_nnn'GTG Cuts at: 2136 2624 3074 2136 Size: 488 450 7439 Fragments arranged by size: 7439 488 450 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7384 809 Size: 507 6068 1802 Fragments arranged by size: 6068 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3471 5824 6713 Size: 54 1425 50 275 18 1641 2353 889 Cuts at: 6713 8342 8 Size: 1629 43 Fragments arranged by size: 2353 1641 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5824 1537 Size: 275 4012 4090 Fragments arranged by size: 4090 4012 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3411 4017 4200 4347 4552 4701 5112 6307 Size: 2058 606 183 147 205 149 411 1195 Cuts at: 6307 1353 Size: 3423 Fragments arranged by size: 3423 2058 1195 606 411 205 183 149 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6964 7338 7484 387 Size: 1064 138 5375 374 146 1280 Fragments arranged by size: 5375 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2060 2525 2886 3014 3630 3666 Size: 393 375 53 465 361 128 616 36 Cuts at: 3666 4020 4865 5294 5670 6440 7037 7824 8092 Size: 354 845 429 376 770 597 787 268 Cuts at: 8092 1239 Size: 1524 Fragments arranged by size: 1524 845 787 770 616 597 465 429 393 376 375 361 354 268 128 53 36 EcoNI CCTnn'n_nnAGG Cuts at: 2078 2078 Size: 8377 EcoO109I rG'GnC_Cy Cuts at: 3653 4441 3653 Size: 788 7589 EcoRI G'AATT_C Cuts at: 2896 3739 4050 4971 5686 6064 2896 Size: 843 311 921 715 378 5209 Fragments arranged by size: 5209 921 843 715 378 311 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4160 5257 4160 Size: 1097 7280 FspI TGC'GCA Cuts at: 6883 6883 Size: 8377 HaeII r_GCGC'y Cuts at: 1678 5151 5524 7524 1678 Size: 3473 373 2000 2531 Fragments arranged by size: 3473 2531 2000 373 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2540 2572 4207 4239 4301 Size: 32 135 32 968 32 1635 32 62 Cuts at: 4301 4313 4333 4345 4445 4477 4482 4514 4844 Size: 12 20 12 100 32 5 32 330 Cuts at: 4844 4876 5254 5281 5286 5313 5424 5456 7018 Size: 32 378 27 5 27 111 32 1562 Cuts at: 7018 7050 7092 7124 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1635 1562 968 378 330 135 111 100 62 42 32 32 32 32 32 32 32 32 32 32 27 27 20 12 12 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 2301 3095 5595 5721 5967 669 Size: 988 281 363 794 2500 126 246 3079 Fragments arranged by size: 3079 2500 988 794 363 281 246 126 HindIII A'AGCT_T Cuts at: 748 3864 4142 5234 748 Size: 3116 278 1092 3891 Fragments arranged by size: 3891 3116 1092 278 HpaI GTT'AAC Cuts at: 5595 5967 5595 Size: 372 8005 KpnI G_GTAC'C Cuts at: 5702 5702 Size: 8377 MfeI C'AATT_G Cuts at: 5976 5976 Size: 8377 MluI A'CGCG_T Cuts at: 8253 8253 Size: 8377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3794 5230 5467 7491 7675 1305 Size: 2489 1436 237 2024 184 2007 Fragments arranged by size: 2489 2024 2007 1436 237 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3473 10 Size: 54 1425 343 1641 4914 Fragments arranged by size: 4914 1641 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4533 5431 6335 6694 6853 518 Size: 648 3367 898 904 359 159 2042 Fragments arranged by size: 3367 2042 904 898 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2850 3023 5143 5793 6470 7618 7863 Size: 433 811 173 2120 650 677 1148 245 Cuts at: 7863 1606 Size: 2120 Fragments arranged by size: 2120 2120 1148 811 677 650 433 245 173 NcoI C'CATG_G Cuts at: 513 1065 3630 513 Size: 552 2565 5260 Fragments arranged by size: 5260 2565 552 NdeI CA'TA_TG Cuts at: 387 2305 387 Size: 1918 6459 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8377 NotI GC'GGCC_GC Cuts at: 5824 5824 Size: 8377 NsiI A_TGCA'T Cuts at: 3440 3440 Size: 8377 NspI r_CATG'y Cuts at: 1132 1229 2371 2539 3059 3442 4657 5101 5735 Size: 97 1142 168 520 383 1215 444 634 Cuts at: 5735 7280 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1215 1142 634 520 444 383 168 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8377 PciI A'CATG_T Cuts at: 4653 5097 7276 4653 Size: 444 2179 5754 Fragments arranged by size: 5754 2179 444 PflMI CCAn_nnn'nTGG Cuts at: 1108 2416 1108 Size: 1308 7069 PfoI T'CCnGG_A Cuts at: 4634 4634 Size: 8377 PmeI GTTT'AAAC Cuts at: 5681 5681 Size: 8377 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4239 4271 6446 6478 7959 7991 4239 Size: 32 2175 32 1481 32 4625 Fragments arranged by size: 4625 2175 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3653 4441 3653 Size: 788 7589 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8377 PsiI TTA'TAA Cuts at: 3312 5947 3312 Size: 2635 5742 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5064 5096 5064 Size: 32 8345 PstI C_TGCA'G Cuts at: 830 2752 2843 3022 5729 830 Size: 1922 91 179 2707 3478 Fragments arranged by size: 3478 2707 1922 179 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8377 PvuII CAG'CTG Cuts at: 2039 2850 3023 5143 2039 Size: 811 173 2120 5273 Fragments arranged by size: 5273 2120 811 173 SalI G'TCGA_C Cuts at: 1936 5719 1936 Size: 3783 4594 SapI GCTCTTCn'nnn_ Cuts at: 4347 4701 5112 4347 Size: 354 411 7612 Fragments arranged by size: 7612 411 354 SbfI CC_TGCA'GG Cuts at: 5729 5729 Size: 8377 ScaI AGT'ACT Cuts at: 3805 4780 6625 8337 3805 Size: 975 1845 1712 3845 Fragments arranged by size: 3845 1845 1712 975 SfcI C'TryA_G Cuts at: 826 1045 2748 2839 2845 3018 3286 3732 4575 Size: 219 1703 91 6 173 268 446 843 Cuts at: 4575 4822 5725 6860 7541 7732 826 Size: 247 903 1135 681 191 1471 Fragments arranged by size: 1703 1471 1135 903 843 681 446 268 247 219 191 173 91 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8377 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8377 SmaI CCC'GGG Cuts at: 1872 5704 1872 Size: 3832 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4175 4782 4800 6481 7382 7644 Size: 197 926 2232 607 18 1681 901 262 Cuts at: 7644 820 Size: 1553 Fragments arranged by size: 2232 1681 1553 926 901 607 262 197 18 SnaBI TAC'GTA Cuts at: 493 6168 493 Size: 5675 2702 SpeI A'CTAG_T Cuts at: 152 2267 152 Size: 2115 6262 SphI G_CATG'C Cuts at: 1132 5735 1132 Size: 4603 3774 SspI AAT'ATT Cuts at: 5 52 3224 3625 6301 5 Size: 47 3172 401 2676 2081 Fragments arranged by size: 3172 2676 2081 401 47 StuI AGG'CCT Cuts at: 1517 4745 1517 Size: 3228 5149 StyI C'CwwG_G Cuts at: 513 1065 3630 3845 4288 4701 4966 5816 513 Size: 552 2565 215 443 413 265 850 3074 Fragments arranged by size: 3074 2565 850 552 443 413 265 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6404 6421 6574 6759 7204 325 Size: 1255 4824 17 153 185 445 1498 Fragments arranged by size: 4824 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2755 3803 4295 Size: 274 80 33 51 1443 778 1048 492 Cuts at: 4295 4778 5277 6623 8335 96 Size: 483 499 1346 1712 138 Fragments arranged by size: 1712 1443 1346 1048 778 499 492 483 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3396 3428 5148 5180 8337 8369 3396 Size: 32 1720 32 3157 32 3404 Fragments arranged by size: 3404 3157 1720 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8377 XbaI T'CTAG_A Cuts at: 4261 5713 4261 Size: 1452 6925 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8377 XmaI C'CCGG_G Cuts at: 1870 5702 1870 Size: 3832 4545 XmnI GAAnn'nnTTC Cuts at: 2873 2896 3542 3673 4143 4257 4415 4854 6506 Size: 23 646 131 470 114 158 439 1652 Cuts at: 6506 2873 Size: 4744 Fragments arranged by size: 4744 1652 646 470 439 158 131 114 23 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6185 276 Size: 53 83 186 936 4651 2468 Fragments arranged by size: 4651 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BplI BsiWI EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI