(Circular) (Six-base) MAPSORT of: pFN21AA0406.seq Check: 5210 from: 1 to: 7972 pFN21AA0406 7972 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3815 3815 Size: 7972 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5782 278 Size: 53 83 186 936 4246 2468 Fragments arranged by size: 4246 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5315 1937 Size: 3378 4594 Acc65I G'GTAC_C Cuts at: 5293 5293 Size: 7972 AclI AA'CG_TT Cuts at: 1563 6099 6472 1563 Size: 4536 373 3063 Fragments arranged by size: 4536 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2132 2204 3023 4443 4673 4676 6035 Size: 491 9 72 819 1420 230 3 1359 Cuts at: 6035 7419 7687 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1420 1384 1359 819 491 268 230 72 9 3 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7775 AflIII A'CryG_T Cuts at: 1203 2258 6871 7848 1203 Size: 1055 4613 977 1327 Fragments arranged by size: 4613 1327 1055 977 AgeI A'CCGG_T Cuts at: 6803 6803 Size: 7972 AhdI GACnn_n'nnGTC Cuts at: 2193 3124 6701 2193 Size: 931 3577 3464 Fragments arranged by size: 3577 3464 931 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7972 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2411 3162 1184 Size: 1227 751 5994 Fragments arranged by size: 5994 1227 751 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4503 4535 4599 4631 4503 Size: 32 64 32 7844 Fragments arranged by size: 7844 64 32 32 AlwNI CAG_nnn'CTG Cuts at: 3602 4072 4972 5715 7287 3602 Size: 470 900 743 1572 4287 Fragments arranged by size: 4287 1572 900 743 470 ApaI G_GGCC'C Cuts at: 3242 4979 3242 Size: 1737 6235 ApaLI G'TGCA_C Cuts at: 6029 7185 6029 Size: 1156 6816 ApoI r'AATT_y Cuts at: 1493 3194 4084 5281 5507 5659 7768 1493 Size: 1701 890 1197 226 152 2109 1697 Fragments arranged by size: 2109 1701 1697 1197 890 226 152 AseI AT'TA_AT Cuts at: 160 1032 2393 6526 160 Size: 872 1361 4133 1606 Fragments arranged by size: 4133 1606 1361 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7972 AvaI C'yCGr_G Cuts at: 1870 1943 5297 1870 Size: 73 3354 4545 Fragments arranged by size: 4545 3354 73 AvrII C'CTAG_G Cuts at: 4999 4999 Size: 7972 BamHI G'GATC_C Cuts at: 5302 5669 5302 Size: 367 7605 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4338 5293 6748 618 Size: 325 217 627 2551 955 1455 1842 Fragments arranged by size: 2551 1842 1455 955 627 325 217 BanII G_rGCy'C Cuts at: 1391 2439 3242 4979 5291 1391 Size: 1048 803 1737 312 4072 Fragments arranged by size: 4072 1737 1048 803 312 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2192 4431 5086 928 Size: 971 293 2239 655 3814 Fragments arranged by size: 3814 2239 971 655 293 BbvCI CC'TCA_GC Cuts at: 3391 3391 Size: 7972 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5227 5261 6163 6197 1903 Size: 34 3290 34 902 34 3678 Fragments arranged by size: 3678 3290 902 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5864 7074 5864 Size: 1210 6762 BclI T'GATC_A Cuts at: 1548 4074 1548 Size: 2526 5446 BfrBI ATG'CAT Cuts at: 3888 3888 Size: 7972 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4775 6583 136 Size: 107 122 71 631 605 3103 1808 1525 Fragments arranged by size: 3103 1808 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2715 7967 2715 Size: 5252 2720 BlpI GC'TnA_GC Cuts at: 5389 5389 Size: 7972 Bme1580I G_kGCm'C Cuts at: 1790 2137 3242 4341 4979 6033 7189 1790 Size: 347 1105 1099 638 1054 1156 2573 Fragments arranged by size: 2573 1156 1105 1099 1054 638 347 BmgBI CAC'GTC Cuts at: 1318 3322 3792 4600 1318 Size: 2004 470 808 4690 Fragments arranged by size: 4690 2004 808 470 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3046 4303 5703 Size: 416 53 83 399 24 1629 1257 1400 Cuts at: 5703 6661 442 Size: 958 1753 Fragments arranged by size: 1753 1629 1400 1257 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7972 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 3263 3632 3995 4005 5234 6632 1239 Size: 1028 996 369 363 10 1229 1398 2579 Fragments arranged by size: 2579 1398 1229 1028 996 369 363 10 Bpu10I CC'TnA_GC Cuts at: 3391 3391 Size: 7972 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3487 4421 5243 6097 6962 7260 3487 Size: 934 822 854 865 298 4199 Fragments arranged by size: 4199 934 865 854 822 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3981 6635 882 Size: 617 2482 2654 2219 Fragments arranged by size: 2654 2482 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4805 5763 493 Size: 711 3601 958 2702 Fragments arranged by size: 3601 2702 958 711 BsaBI GATnn'nnATC Cuts at: 4635 7966 4635 Size: 3331 4641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4767 5779 6161 6796 Size: 53 83 186 936 3234 1012 382 635 Cuts at: 6796 275 Size: 1451 Fragments arranged by size: 3234 1451 1012 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3558 6405 6803 7077 7224 7635 3558 Size: 2847 398 274 147 411 3895 Fragments arranged by size: 3895 2847 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5108 5138 1178 Size: 30 165 30 3705 30 4012 Fragments arranged by size: 4012 3705 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2369 3436 3767 1186 Size: 856 327 1067 331 5391 Fragments arranged by size: 5391 1067 856 331 327 BseYI C'CCAG_C Cuts at: 2324 2608 3605 5183 7175 7671 2324 Size: 284 997 1578 1992 496 2625 Fragments arranged by size: 2625 1992 1578 997 496 284 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2634 2986 5183 5189 5219 1163 Size: 573 898 352 2197 6 30 3916 Fragments arranged by size: 3916 2197 898 573 352 30 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5422 6183 7211 1540 Size: 275 186 3421 761 1028 2301 Fragments arranged by size: 3421 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2802 3771 4412 5250 6033 6118 7189 2802 Size: 969 641 838 783 85 1071 3585 Fragments arranged by size: 3585 1071 969 838 783 641 85 BsmI GAATG_Cn' Cuts at: 2237 3139 3379 3888 5483 5576 2237 Size: 902 240 509 1595 93 4633 Fragments arranged by size: 4633 1595 902 509 240 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2439 2802 3242 3771 4341 4412 Size: 399 347 302 363 440 529 570 71 Cuts at: 4412 4979 5250 5291 6033 6118 7189 1391 Size: 567 271 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 570 567 529 440 399 363 347 302 271 85 71 41 BspEI T'CCGG_A Cuts at: 7635 7635 Size: 7972 BspHI T'CATG_A Cuts at: 5861 7591 5861 Size: 1730 6242 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2347 2797 3463 3815 4200 5260 5327 844 Size: 1503 450 666 352 385 1060 67 3489 Fragments arranged by size: 3489 1503 1060 666 450 385 352 67 BsrBI CCG'CTC Cuts at: 1394 1823 5859 1394 Size: 429 4036 3507 Fragments arranged by size: 4036 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4574 6467 6641 65 Size: 1183 3326 1893 174 1396 Fragments arranged by size: 3326 1893 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4842 6616 6803 1700 Size: 110 3032 1774 187 2869 Fragments arranged by size: 3032 2869 1774 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7972 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7972 BssSI C'ACGA_G Cuts at: 6032 7044 6032 Size: 1012 6960 BstAPI GCAn_nnn'nTGC Cuts at: 794 2789 794 Size: 1995 5977 BstBI TT'CG_AA Cuts at: 6776 6776 Size: 7972 BstEII G'GTnAC_C Cuts at: 1184 3721 5009 1184 Size: 2537 1288 4147 Fragments arranged by size: 4147 2537 1288 BstYI r'GATC_y Cuts at: 1972 2715 3622 4107 4581 4672 5302 5669 6054 Size: 743 907 485 474 91 630 367 385 Cuts at: 6054 6071 7523 7609 7621 7967 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 907 743 630 485 474 385 367 346 91 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2025 2025 Size: 7972 BtgI C'CryG_G Cuts at: 513 1065 2004 4593 513 Size: 552 939 2589 3892 Fragments arranged by size: 3892 2589 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7233 Fragments arranged by size: 7233 683 56 BtsI GCAGTG_nn' Cuts at: 2561 4568 4785 5488 6282 6302 2561 Size: 2007 217 703 794 20 4231 Fragments arranged by size: 4231 2007 794 703 217 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7972 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4557 4592 529 Size: 35 3993 35 3909 Fragments arranged by size: 3993 3909 35 35 DraI TTT'AAA Cuts at: 2680 4116 5276 2680 Size: 1436 1160 5376 Fragments arranged by size: 5376 1436 1160 DraIII CAC_nnn'GTG Cuts at: 5197 5197 Size: 7972 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6979 809 Size: 507 5663 1802 Fragments arranged by size: 5663 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3907 4230 5419 Size: 54 1425 50 275 18 2077 323 1189 Cuts at: 5419 6308 7937 8 Size: 889 1629 43 Fragments arranged by size: 2077 1629 1425 1189 889 323 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5419 1537 Size: 275 3607 4090 Fragments arranged by size: 4090 3607 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2168 3162 4359 4400 4500 5902 1353 Size: 815 994 1197 41 100 1402 3423 Fragments arranged by size: 3423 1402 1197 994 815 100 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4637 6559 6933 7079 387 Size: 1064 138 3048 1922 374 146 1280 Fragments arranged by size: 3048 1922 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2132 2204 2267 3023 3263 3632 Size: 393 491 9 72 63 756 240 369 Cuts at: 3632 3995 4005 4443 4673 4676 5234 6035 6632 Size: 363 10 438 230 3 558 801 597 Cuts at: 6632 7419 7687 1239 Size: 787 268 1524 Fragments arranged by size: 1524 801 787 756 597 558 491 438 393 369 363 268 240 230 72 63 10 9 3 EcoNI CCTnn'n_nnAGG Cuts at: 3002 5003 3002 Size: 2001 5971 EcoO109I rG'GnC_Cy Cuts at: 2195 2950 4156 4894 4969 4976 2195 Size: 755 1206 738 75 7 5191 Fragments arranged by size: 5191 1206 755 738 75 7 EcoRI G'AATT_C Cuts at: 5281 5659 5281 Size: 378 7594 FspI TGC'GCA Cuts at: 6478 6478 Size: 7972 HaeII r_GCGC'y Cuts at: 1678 7119 1678 Size: 5441 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2179 2211 3110 3142 4499 Size: 32 135 32 607 32 899 32 1357 Cuts at: 4499 4531 5108 5140 6613 6645 6687 6719 1373 Size: 32 577 32 1473 32 42 32 2626 Fragments arranged by size: 2626 1473 1357 899 607 577 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2865 5316 5562 669 Size: 988 281 927 2451 246 3079 Fragments arranged by size: 3079 2451 988 927 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7972 HpaI GTT'AAC Cuts at: 5562 5562 Size: 7972 KpnI G_GTAC'C Cuts at: 5297 5297 Size: 7972 MfeI C'AATT_G Cuts at: 5571 5571 Size: 7972 MluI A'CGCG_T Cuts at: 7848 7848 Size: 7972 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3335 3876 7086 7270 1305 Size: 2030 541 3210 184 2007 Fragments arranged by size: 3210 2030 2007 541 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3909 4232 10 Size: 54 1425 343 2077 323 3750 Fragments arranged by size: 3750 2077 1425 343 323 54 MslI CAynn'nnrTG Cuts at: 518 1166 2393 4028 4598 5930 6289 6448 518 Size: 648 1227 1635 570 1332 359 159 2042 Fragments arranged by size: 2042 1635 1332 1227 648 570 359 159 MspA1I CmG'CkG Cuts at: 1606 2608 3283 4421 4711 4954 5203 5217 5254 Size: 1002 675 1138 290 243 249 14 37 Cuts at: 5254 5388 6065 7213 7458 1606 Size: 134 677 1148 245 2120 Fragments arranged by size: 2120 1148 1138 1002 677 675 290 249 245 243 134 37 14 NcoI C'CATG_G Cuts at: 513 1065 2004 4593 513 Size: 552 939 2589 3892 Fragments arranged by size: 3892 2589 939 552 NdeI CA'TA_TG Cuts at: 387 3919 387 Size: 3532 4440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7972 NotI GC'GGCC_GC Cuts at: 5419 5419 Size: 7972 NsiI A_TGCA'T Cuts at: 3890 3890 Size: 7972 NspI r_CATG'y Cuts at: 1132 1229 2262 4289 5330 6875 1132 Size: 97 1033 2027 1041 1545 2229 Fragments arranged by size: 2229 2027 1545 1041 1033 97 PasI CC'CwG_GG Cuts at: 1348 4810 1348 Size: 3462 4510 PciI A'CATG_T Cuts at: 2258 6871 2258 Size: 4613 3359 PflMI CCAn_nnn'nTGG Cuts at: 1108 2083 2224 1108 Size: 975 141 6856 Fragments arranged by size: 6856 975 141 PfoI T'CCnGG_A Cuts at: 2283 4158 2283 Size: 1875 6097 PmeI GTTT'AAAC Cuts at: 5276 5276 Size: 7972 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6041 6073 7554 7586 6041 Size: 32 1481 32 6427 Fragments arranged by size: 6427 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2195 2950 4156 2195 Size: 755 1206 6011 Fragments arranged by size: 6011 1206 755 PshAI GACnn'nnGTC Cuts at: 1613 4548 1613 Size: 2935 5037 PsiI TTA'TAA Cuts at: 5542 5542 Size: 7972 PspOMI G'GGCC_C Cuts at: 3238 4975 3238 Size: 1737 6235 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5135 5167 5135 Size: 32 7940 PstI C_TGCA'G Cuts at: 830 2106 2794 4068 5271 5324 830 Size: 1276 688 1274 1203 53 3478 Fragments arranged by size: 3478 1276 1274 1203 688 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7972 PvuII CAG'CTG Cuts at: 2608 4421 4711 4954 5203 5254 2608 Size: 1813 290 243 249 51 5326 Fragments arranged by size: 5326 1813 290 249 243 51 SalI G'TCGA_C Cuts at: 1936 5314 1936 Size: 3378 4594 SapI GCTCTTCn'nnn_ Cuts at: 4400 4400 Size: 7972 SbfI CC_TGCA'GG Cuts at: 5324 5324 Size: 7972 ScaI AGT'ACT Cuts at: 3972 6220 7932 3972 Size: 2248 1712 4012 Fragments arranged by size: 4012 2248 1712 SexAI A'CCwGG_T Cuts at: 5129 5129 Size: 7972 SfcI C'TryA_G Cuts at: 826 1045 2102 2790 4064 5267 5320 6455 7136 Size: 219 1057 688 1274 1203 53 1135 681 Cuts at: 7136 7327 826 Size: 191 1471 Fragments arranged by size: 1471 1274 1203 1135 1057 688 681 219 191 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7972 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7972 SmaI CCC'GGG Cuts at: 1872 5299 1872 Size: 3427 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3466 4400 5258 6076 6977 7239 Size: 197 926 1523 934 858 818 901 262 Cuts at: 7239 820 Size: 1553 Fragments arranged by size: 1553 1523 934 926 901 858 818 262 197 SnaBI TAC'GTA Cuts at: 493 4805 5763 493 Size: 4312 958 2702 Fragments arranged by size: 4312 2702 958 SpeI A'CTAG_T Cuts at: 152 3019 152 Size: 2867 5105 SphI G_CATG'C Cuts at: 1132 4289 5330 1132 Size: 3157 1041 3774 Fragments arranged by size: 3774 3157 1041 SspI AAT'ATT Cuts at: 5 52 5896 5 Size: 47 5844 2081 Fragments arranged by size: 5844 2081 47 StuI AGG'CCT Cuts at: 1517 2033 2484 3008 3777 4733 1517 Size: 516 451 524 769 956 4756 Fragments arranged by size: 4756 956 769 524 516 451 StyI C'CwwG_G Cuts at: 513 1065 2004 2576 3404 4593 4719 4999 5411 Size: 552 939 572 828 1189 126 280 412 Cuts at: 5411 513 Size: 3074 Fragments arranged by size: 3074 1189 939 828 572 552 412 280 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2338 2922 3786 4904 5999 6016 6169 Size: 1255 758 584 864 1118 1095 17 153 Cuts at: 6169 6354 6799 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1118 1095 864 758 584 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2154 3970 6218 Size: 274 80 33 51 1443 177 1816 2248 Cuts at: 6218 7930 96 Size: 1712 138 Fragments arranged by size: 2248 1816 1712 1443 274 177 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3142 3174 7932 7964 3142 Size: 32 4758 32 3150 Fragments arranged by size: 4758 3150 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7972 XbaI T'CTAG_A Cuts at: 3310 5308 3310 Size: 1998 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4027 4600 5127 4027 Size: 573 527 6872 Fragments arranged by size: 6872 573 527 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7972 XmaI C'CCGG_G Cuts at: 1870 5297 1870 Size: 3427 4545 XmnI GAAnn'nnTTC Cuts at: 6101 6101 Size: 7972 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5780 276 Size: 53 83 186 936 4246 2468 Fragments arranged by size: 4246 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI