(Circular) (Six-base) MAPSORT of: pFN21AA0397.seq Check: 6010 from: 1 to: 7723 pFN21AA0397 7723 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3090 3775 3808 3090 Size: 685 33 7005 Fragments arranged by size: 7005 685 33 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3729 4470 5533 278 Size: 53 83 186 936 2193 741 1063 2468 Fragments arranged by size: 2468 2193 1063 936 741 186 83 53 AccI GT'mk_AC Cuts at: 1937 5066 1937 Size: 3129 4594 Acc65I G'GTAC_C Cuts at: 3810 5044 3810 Size: 1234 6489 AclI AA'CG_TT Cuts at: 1563 5850 6223 1563 Size: 4287 373 3063 Fragments arranged by size: 4287 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2897 2953 4038 4567 5786 7170 7438 1632 Size: 1265 56 1085 529 1219 1384 268 1917 Fragments arranged by size: 1917 1384 1265 1219 1085 529 268 56 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7526 AflIII A'CryG_T Cuts at: 1203 2389 3627 4482 6622 7599 1203 Size: 1186 1238 855 2140 977 1327 Fragments arranged by size: 2140 1327 1238 1186 977 855 AgeI A'CCGG_T Cuts at: 3444 6554 3444 Size: 3110 4613 AhdI GACnn_n'nnGTC Cuts at: 6452 6452 Size: 7723 AleI CACnn'nnGTG Cuts at: 1166 2225 3020 4073 1166 Size: 1059 795 1053 4816 Fragments arranged by size: 4816 1059 1053 795 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2874 3038 4971 1184 Size: 1690 164 1933 3936 Fragments arranged by size: 3936 1933 1690 164 AlwNI CAG_nnn'CTG Cuts at: 2278 2498 3426 4675 5466 7038 2278 Size: 220 928 1249 791 1572 2963 Fragments arranged by size: 2963 1572 1249 928 791 220 ApaI G_GGCC'C Cuts at: 3330 4239 3330 Size: 909 6814 ApaLI G'TGCA_C Cuts at: 3566 5780 6936 3566 Size: 2214 1156 4353 Fragments arranged by size: 4353 2214 1156 ApoI r'AATT_y Cuts at: 1493 5032 5258 5410 7519 1493 Size: 3539 226 152 2109 1697 Fragments arranged by size: 3539 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6277 160 Size: 872 5245 1606 Fragments arranged by size: 5245 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7723 AvaI C'yCGr_G Cuts at: 1870 1943 3123 3495 3598 4055 4153 4451 4980 Size: 73 1180 372 103 457 98 298 529 Cuts at: 4980 5048 1870 Size: 68 4545 Fragments arranged by size: 4545 1180 529 457 372 298 103 98 73 68 BamHI G'GATC_C Cuts at: 2676 4273 5053 5420 2676 Size: 1597 780 367 4979 Fragments arranged by size: 4979 1597 780 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2616 2962 3345 3810 4067 Size: 325 217 627 829 346 383 465 257 Cuts at: 4067 4534 4628 5044 6499 618 Size: 467 94 416 1455 1842 Fragments arranged by size: 1842 1455 829 627 467 465 416 383 346 325 257 217 94 BanII G_rGCy'C Cuts at: 1391 2345 3141 3330 3357 3374 4008 4239 4269 Size: 954 796 189 27 17 634 231 30 Cuts at: 4269 5042 1391 Size: 773 4072 Fragments arranged by size: 4072 954 796 773 634 231 189 30 27 17 BbeI G_GCGC'C Cuts at: 4538 4538 Size: 7723 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2115 2240 928 Size: 971 216 125 6411 Fragments arranged by size: 6411 971 216 125 BbvCI CC'TCA_GC Cuts at: 2369 2369 Size: 7723 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 5914 5948 1903 Size: 34 1961 34 1982 34 3678 Fragments arranged by size: 3678 1982 1961 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5615 6825 5615 Size: 1210 6513 BclI T'GATC_A Cuts at: 1548 4561 1548 Size: 3013 4710 BfrBI ATG'CAT Cuts at: 4702 4702 Size: 7723 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3039 3343 3457 Size: 107 122 71 631 605 1367 304 114 Cuts at: 3457 6334 136 Size: 2877 1525 Fragments arranged by size: 2877 1525 1367 631 605 304 122 114 107 71 BglII A'GATC_T Cuts at: 7718 7718 Size: 7723 BlpI GC'TnA_GC Cuts at: 2344 4944 5140 2344 Size: 2600 196 4927 Fragments arranged by size: 4927 2600 196 Bme1580I G_kGCm'C Cuts at: 1790 2243 2734 2967 3233 3330 3570 4070 4239 Size: 453 491 233 266 97 240 500 169 Cuts at: 4239 5784 6940 1790 Size: 1545 1156 2573 Fragments arranged by size: 2573 1545 1156 500 491 453 266 240 233 169 97 BmgBI CAC'GTC Cuts at: 1318 2238 3628 4501 1318 Size: 920 1390 873 4540 Fragments arranged by size: 4540 1390 920 873 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2237 2953 5454 Size: 416 53 83 399 24 820 716 2501 Cuts at: 5454 6412 442 Size: 958 1753 Fragments arranged by size: 2501 1753 958 820 716 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7723 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2889 4123 4243 2889 Size: 1234 120 6369 Fragments arranged by size: 6369 1234 120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3125 3371 3379 3692 4095 4213 4328 6383 Size: 1886 246 8 313 403 118 115 2055 Cuts at: 6383 1239 Size: 2579 Fragments arranged by size: 2579 2055 1886 403 313 246 118 115 8 Bpu10I CC'TnA_GC Cuts at: 2369 3455 4038 2369 Size: 1086 583 6054 Fragments arranged by size: 6054 1086 583 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2546 2677 5848 6713 7011 2546 Size: 131 3171 865 298 3258 Fragments arranged by size: 3258 3171 865 298 131 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6386 882 Size: 617 4887 2219 Fragments arranged by size: 4887 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2392 2797 3928 4483 5514 493 Size: 711 1188 405 1131 555 1031 2702 Fragments arranged by size: 2702 1188 1131 1031 711 555 405 BsaBI GATnn'nnATC Cuts at: 7717 7717 Size: 7723 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2173 3726 4467 4535 Size: 53 83 186 936 640 1553 741 68 Cuts at: 4535 5530 5912 6547 275 Size: 995 382 635 1451 Fragments arranged by size: 1553 1451 995 936 741 640 635 382 186 83 68 53 BsaWI w'CCGG_w Cuts at: 2679 3258 3444 6156 6554 6828 6975 7386 2679 Size: 579 186 2712 398 274 147 411 3016 Fragments arranged by size: 3016 2712 579 411 398 274 186 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3344 3374 3935 3965 4643 Size: 30 165 30 1941 30 561 30 678 Cuts at: 4643 4673 1178 Size: 30 4228 Fragments arranged by size: 4228 1941 678 561 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 3236 3364 3382 3892 4120 4217 4334 Size: 907 1143 128 18 510 228 97 117 Cuts at: 4334 1186 Size: 4575 Fragments arranged by size: 4575 1143 907 510 228 128 117 97 18 BseYI C'CCAG_C Cuts at: 2665 3153 3329 3419 3880 4277 6926 7422 2665 Size: 488 176 90 461 397 2649 496 2966 Fragments arranged by size: 2966 2649 496 488 461 397 176 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2160 2738 2825 2834 3382 3950 4370 Size: 573 424 578 87 9 548 568 420 Cuts at: 4370 4427 4495 4532 5021 1163 Size: 57 68 37 489 3865 Fragments arranged by size: 3865 578 573 568 548 489 424 420 87 68 57 37 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3445 5173 5934 6962 1540 Size: 275 186 1444 1728 761 1028 2301 Fragments arranged by size: 2301 1728 1444 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3291 3570 3711 4494 4839 5784 5869 6940 3291 Size: 279 141 783 345 945 85 1071 4074 Fragments arranged by size: 4074 1071 945 783 345 279 141 85 BsmI GAATG_Cn' Cuts at: 3201 4090 5234 5327 3201 Size: 889 1144 93 5597 Fragments arranged by size: 5597 1144 889 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2164 4458 2164 Size: 2294 5429 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2243 2345 2734 2967 3141 3233 3291 Size: 399 453 102 389 233 174 92 58 Cuts at: 3291 3330 3357 3374 3570 3711 4008 4070 4239 Size: 39 27 17 196 141 297 62 169 Cuts at: 4239 4269 4494 4839 5042 5784 5869 6940 1391 Size: 30 225 345 203 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 453 399 389 345 297 233 225 203 196 174 169 141 102 92 85 62 58 39 30 27 17 BspEI T'CCGG_A Cuts at: 2679 3258 7386 2679 Size: 579 4128 3016 Fragments arranged by size: 4128 3016 579 BspHI T'CATG_A Cuts at: 2898 4593 5612 7342 2898 Size: 1695 1019 1730 3279 Fragments arranged by size: 3279 1730 1695 1019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3090 3775 3808 4392 5078 844 Size: 2246 685 33 584 686 3489 Fragments arranged by size: 3489 2246 686 685 584 33 BsrBI CCG'CTC Cuts at: 1394 1823 3009 3454 3868 5610 1394 Size: 429 1186 445 414 1742 3507 Fragments arranged by size: 3507 1742 1186 445 429 414 BsrDI GCAATG_nn' Cuts at: 65 1248 2124 6218 6392 65 Size: 1183 876 4094 174 1396 Fragments arranged by size: 4094 1396 1183 876 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3444 3591 6367 6554 1700 Size: 110 1634 147 2776 187 2869 Fragments arranged by size: 2869 2776 1634 187 147 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7723 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7723 BssSI C'ACGA_G Cuts at: 2957 3284 3710 5783 6795 2957 Size: 327 426 2073 1012 3885 Fragments arranged by size: 3885 2073 1012 426 327 BstAPI GCAn_nnn'nTGC Cuts at: 794 2991 794 Size: 2197 5526 BstBI TT'CG_AA Cuts at: 4197 6527 4197 Size: 2330 5393 BstEII G'GTnAC_C Cuts at: 1184 4546 1184 Size: 3362 4361 BstYI r'GATC_y Cuts at: 1972 2676 3714 4273 4671 4965 5053 5420 5805 Size: 704 1038 559 398 294 88 367 385 Cuts at: 5805 5822 7274 7360 7372 7718 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1038 704 559 398 385 367 346 294 88 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2308 2376 3904 4293 2308 Size: 68 1528 389 5738 Fragments arranged by size: 5738 1528 389 68 BtgI C'CryG_G Cuts at: 513 1065 2004 3210 3537 3609 4074 4095 4146 Size: 552 939 1206 327 72 465 21 51 Cuts at: 4146 4632 513 Size: 486 3604 Fragments arranged by size: 3604 1206 939 552 486 465 327 72 51 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4837 487 Size: 683 56 3611 3373 Fragments arranged by size: 3611 3373 683 56 BtsI GCAGTG_nn' Cuts at: 2148 2203 2483 2989 3520 3801 5239 6033 6053 Size: 55 280 506 531 281 1438 794 20 Cuts at: 6053 2148 Size: 3818 Fragments arranged by size: 3818 1438 794 531 506 281 280 55 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7723 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7688 DraI TTT'AAA Cuts at: 5027 5027 Size: 7723 DraIII CAC_nnn'GTG Cuts at: 4999 4999 Size: 7723 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3553 6730 809 Size: 507 2237 3177 1802 Fragments arranged by size: 3177 2237 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3274 3745 5170 Size: 54 1425 50 275 18 1444 471 1425 Cuts at: 5170 6059 7688 8 Size: 889 1629 43 Fragments arranged by size: 1629 1444 1425 1425 889 471 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5170 1537 Size: 275 3358 4090 Fragments arranged by size: 4090 3358 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2529 3473 4194 4592 5653 1353 Size: 1176 944 721 398 1061 3423 Fragments arranged by size: 3423 1176 1061 944 721 398 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2465 6310 6684 6830 387 Size: 1064 138 876 3845 374 146 1280 Fragments arranged by size: 3845 1280 1064 876 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2897 2953 3125 3371 3379 3692 4038 Size: 393 1265 56 172 246 8 313 346 Cuts at: 4038 4095 4213 4328 4567 5786 6383 7170 7438 Size: 57 118 115 239 1219 597 787 268 Cuts at: 7438 1239 Size: 1524 Fragments arranged by size: 1524 1265 1219 787 597 393 346 313 268 246 239 172 118 115 57 56 8 EcoNI CCTnn'n_nnAGG Cuts at: 3620 4175 4868 3620 Size: 555 693 6475 Fragments arranged by size: 6475 693 555 EcoO109I rG'GnC_Cy Cuts at: 2326 2359 2534 3603 4012 4052 4984 2326 Size: 33 175 1069 409 40 932 5065 Fragments arranged by size: 5065 1069 932 409 175 40 33 EcoRI G'AATT_C Cuts at: 5032 5410 5032 Size: 378 7345 FspI TGC'GCA Cuts at: 2195 6229 2195 Size: 4034 3689 HaeII r_GCGC'y Cuts at: 1678 2411 2665 3563 3937 4538 6870 1678 Size: 733 254 898 374 601 2332 2531 Fragments arranged by size: 2531 2332 898 733 601 374 254 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2557 2589 3625 3657 4872 Size: 32 135 32 985 32 1036 32 1215 Cuts at: 4872 4904 6364 6396 6438 6470 1373 Size: 32 1460 32 42 32 2626 Fragments arranged by size: 2626 1460 1215 1036 985 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5067 5313 669 Size: 988 281 3129 246 3079 Fragments arranged by size: 3129 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7723 HpaI GTT'AAC Cuts at: 5313 5313 Size: 7723 KasI G'GCGC_C Cuts at: 4534 4534 Size: 7723 KpnI G_GTAC'C Cuts at: 3814 5048 3814 Size: 1234 6489 MfeI C'AATT_G Cuts at: 5322 5322 Size: 7723 MluI A'CGCG_T Cuts at: 7599 7599 Size: 7723 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2688 6837 7021 1305 Size: 1383 4149 184 2007 Fragments arranged by size: 4149 2007 1383 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3747 10 Size: 54 1425 343 1915 3986 Fragments arranged by size: 3986 1915 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2225 3020 4073 4707 4953 5681 6040 Size: 648 1059 795 1053 634 246 728 359 Cuts at: 6040 6199 518 Size: 159 2042 Fragments arranged by size: 2042 1059 1053 795 728 648 634 359 246 159 MspA1I CmG'CkG Cuts at: 1606 2167 2183 2352 2614 3058 3145 3611 3862 Size: 561 16 169 262 444 87 466 251 Cuts at: 3862 4567 4578 5139 5816 6964 7209 1606 Size: 705 11 561 677 1148 245 2120 Fragments arranged by size: 2120 1148 705 677 561 561 466 444 262 251 245 169 87 16 11 NarI GG'CG_CC Cuts at: 4535 4535 Size: 7723 NcoI C'CATG_G Cuts at: 513 1065 2004 4632 513 Size: 552 939 2628 3604 Fragments arranged by size: 3604 2628 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7723 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7723 NotI GC'GGCC_GC Cuts at: 5170 5170 Size: 7723 NsiI A_TGCA'T Cuts at: 4704 4704 Size: 7723 NspI r_CATG'y Cuts at: 1132 1229 3413 4522 4657 5081 6626 1132 Size: 97 2184 1109 135 424 1545 2229 Fragments arranged by size: 2229 2184 1545 1109 424 135 97 PasI CC'CwG_GG Cuts at: 1348 2671 1348 Size: 1323 6400 PciI A'CATG_T Cuts at: 6622 6622 Size: 7723 PflMI CCAn_nnn'nTGG Cuts at: 1108 2800 4675 1108 Size: 1692 1875 4156 Fragments arranged by size: 4156 1875 1692 PfoI T'CCnGG_A Cuts at: 4674 4674 Size: 7723 PmeI GTTT'AAAC Cuts at: 5027 5027 Size: 7723 PmlI CAC'GTG Cuts at: 2797 3928 2797 Size: 1131 6592 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5792 5824 7305 7337 5792 Size: 32 1481 32 6178 Fragments arranged by size: 6178 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2534 4012 4052 4984 2534 Size: 1478 40 932 5273 Fragments arranged by size: 5273 1478 932 40 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7723 PsiI TTA'TAA Cuts at: 5293 5293 Size: 7723 PspOMI G'GGCC_C Cuts at: 3326 4235 3326 Size: 909 6814 PstI C_TGCA'G Cuts at: 830 2283 2462 2863 4249 5075 830 Size: 1453 179 401 1386 826 3478 Fragments arranged by size: 3478 1453 1386 826 401 179 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7723 PvuII CAG'CTG Cuts at: 2167 4567 4578 2167 Size: 2400 11 5312 Fragments arranged by size: 5312 2400 11 SacII CC_GC'GG Cuts at: 3612 3612 Size: 7723 SalI G'TCGA_C Cuts at: 1936 5065 1936 Size: 3129 4594 SanDI GG'GwC_CC Cuts at: 2534 2534 Size: 7723 SbfI CC_TGCA'GG Cuts at: 5075 5075 Size: 7723 ScaI AGT'ACT Cuts at: 2468 5971 7683 2468 Size: 3503 1712 2508 Fragments arranged by size: 3503 2508 1712 SfcI C'TryA_G Cuts at: 826 1045 2279 2458 2859 3427 4245 4353 4573 Size: 219 1234 179 401 568 818 108 220 Cuts at: 4573 5071 6206 6887 7078 826 Size: 498 1135 681 191 1471 Fragments arranged by size: 1471 1234 1135 818 681 568 498 401 220 219 191 179 108 SfoI GGC'GCC Cuts at: 4536 4536 Size: 7723 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7723 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7723 SmaI CCC'GGG Cuts at: 1872 3497 3600 4057 4982 5050 1872 Size: 1625 103 457 925 68 4545 Fragments arranged by size: 4545 1625 925 457 103 68 SmlI C'TyrA_G Cuts at: 820 1017 1943 2561 2692 4451 5827 6728 6990 Size: 197 926 618 131 1759 1376 901 262 Cuts at: 6990 820 Size: 1553 Fragments arranged by size: 1759 1553 1376 926 901 618 262 197 131 SnaBI TAC'GTA Cuts at: 493 5514 493 Size: 5021 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7723 SphI G_CATG'C Cuts at: 1132 5081 1132 Size: 3949 3774 SspI AAT'ATT Cuts at: 5 52 5647 5 Size: 47 5595 2081 Fragments arranged by size: 5595 2081 47 StuI AGG'CCT Cuts at: 1517 2381 3839 4874 1517 Size: 864 1458 1035 4366 Fragments arranged by size: 4366 1458 1035 864 StyI C'CwwG_G Cuts at: 513 1065 2004 2223 2544 3621 4632 5162 513 Size: 552 939 219 321 1077 1011 530 3074 Fragments arranged by size: 3074 1077 1011 939 552 530 321 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3894 4655 4736 5750 5767 5920 6105 Size: 1255 2314 761 81 1014 17 153 185 Cuts at: 6105 6550 325 Size: 445 1498 Fragments arranged by size: 2314 1498 1255 1014 761 445 185 153 81 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2466 5969 7681 Size: 274 80 33 51 1443 489 3503 1712 Cuts at: 7681 96 Size: 138 Fragments arranged by size: 3503 1712 1443 489 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3071 3103 3344 3376 3935 3967 7683 7715 3071 Size: 32 241 32 559 32 3716 32 3079 Fragments arranged by size: 3716 3079 559 241 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2024 2432 4988 1316 Size: 708 408 2556 4051 Fragments arranged by size: 4051 2556 708 408 XbaI T'CTAG_A Cuts at: 5059 5059 Size: 7723 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2900 2900 Size: 7723 XhoI C'TCGA_G Cuts at: 1943 4451 1943 Size: 2508 5215 XmaI C'CCGG_G Cuts at: 1870 3495 3598 4055 4980 5048 1870 Size: 1625 103 457 925 68 4545 Fragments arranged by size: 4545 1625 925 457 103 68 XmnI GAAnn'nnTTC Cuts at: 3173 5852 3173 Size: 2679 5044 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3727 4468 5531 276 Size: 53 83 186 936 2193 741 1063 2468 Fragments arranged by size: 2468 2193 1063 936 741 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI