Restriction map for
pFN21AA0389
##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI AspEI AspI AviII
AvrII BbrPI BcgI BciVI BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII Bst1107I BstUI BstZ17I CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
KspI MamI MluI MroI MvnI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI Tsp45I
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1224 GGTACC G^GTACC
AflII 901 CTTAAG C^TTAAG
AflIII 2147 ACATGT A^C[GA][CT]GT
Alw44I 960 GTGCAC G^TGCAC
AocI 3039 CCTGAGG CC^T[ACGT]AGG
ApaLI 960 GTGCAC G^TGCAC
Asp718I 1224 GGTACC G^GTACC
AvaI 2593 CCCGAG C^[CT]CG[GA]G
BamHI 2187 GGATCC G^GATCC
BbeI 24 GGCGCC GGCGC^C
BfrI 901 CTTAAG C^TTAAG
BglI 3088 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3728 AGATCT A^GATCT
BsaAI 3254 TACGTG [CT]AC^GT[GA]
BsaWI 3135 ACCGGA [AT]^CCGG[AT]
BsmBI 1715 GAGACG CGTCTC(1/5)
BsoBI 2593 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2150 TGTACA T^GTACA
BspLU11I 2147 ACATGT A^CATGT
BspMI 907 GCAGGT ACCTGC(4/8)
BsrDI 1427 GCAATG GCAATG(2/0)
BsrFI 463 GCCGGC [GA]^CCGG[CT]
BsrGI 2150 TGTACA T^GTACA
BstAPI 1246 GCAAACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2110 GGTTACC G^GT[ACGT]ACC
BstPI 2110 GGTTACC G^GT[ACGT]ACC
Bsu36I 3039 CCTGAGG CC^T[ACGT]AGG
Cfr10I 463 GCCGGC [GA]^CCGG[CT]
DraII 3177 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 3039 CCTGAGG CC^T[ACGT]AGG
EcoO109I 3177 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2110 GGTTACC G^GT[ACGT]ACC
HincII 2256 GTTAAC GT[CT]^[GA]AC
HindII 2256 GTTAAC GT[CT]^[GA]AC
HpaI 2256 GTTAAC GTT^AAC
KasI 24 GGCGCC G^GCGCC
KpnI 1224 GGTACC GGTAC^C
NaeI 463 GCCGGC GCC^GGC
NarI 24 GGCGCC GG^CGCC
NgoMIV 463 GCCGGC G^CCGGC
PflMI 1773 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3177 AGGTCCT [GA]G^G[AT]CC[CT]
SfoI 24 GGCGCC GGC^GCC
SspBI 2150 TGTACA T^GTACA
SwaI 200 ATTTAAAT ATTT^AAAT
Van91I 1773 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3641 CCATCCTACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 24 GGCGCC G[GA]^CG[CT]C
1027 GGCGTC
BalI 2645 TGGCCA TGG^CCA
3146 TGGCCA
BbsI 585 GAAGAC GAAGAC(2/6)
2824 GAAGAC
BbvCI 3439 CCTCAGC CCTCAGC(-5/-2)
2775 GCTGAGG
BpuAI 585 GAAGAC GAAGAC(2/6)
2824 GAAGAC
BsaHI 24 GGCGCC G[GA]^CG[CT]C
1027 GGCGTC
BsgI 831 CTGCAC GTGCAG(16/14)
1033 CTGCAC
BsmFI 361 GGGAC GGGAC(10/14)
3023 GGGAC
BsmI 3789 GAATGC GAATGC(1/-1)
2939 GCATTC
BspHI 2133 TCATGA T^CATGA
2306 TCATGA
BssSI 2120 CACGAG CACGAG(-5/-1)
1256 CTCGTG
BstBI 1973 TTCGAA TT^CGAA
2471 TTCGAA
BstXI 1559 CCAAATTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3585 CCATTTTGATGG
DsaI 939 CCATGG C^C[GA][CT]GG
3598 CCATGG
EaeI 2645 TGGCCA [CT]^GGCC[GA]
3146 TGGCCA
FauI 1747 GCGGG CCCGC(4/6)
3477 GCGGG
HaeII 24 GGCGCC [GA]GCGC^[CT]
3096 GGCGCT
HgaI 2670 GACGC GACGC(5/10)
1028 GCGTC
Hin1I 24 GGCGCC G[GA]^CG[CT]C
1027 GGCGTC
MaeIII 2111 GTTAC ^GT[ACGT]AC
3400 GTTAC
MfeI 89 CAATTG C^AATTG
386 CAATTG
MluNI 2645 TGGCCA TGG^CCA
3146 TGGCCA
MscI 2645 TGGCCA TGG^CCA
3146 TGGCCA
MunI 89 CAATTG C^AATTG
386 CAATTG
NcoI 939 CCATGG C^CATGG
3598 CCATGG
NdeI 263 CATATG CA^TATG
1804 CATATG
NspI 2147 ACATGT [GA]CATG^[CT]
2365 ACATGC
NspV 1973 TTCGAA TT^CGAA
2471 TTCGAA
PvuII 2962 CAGCTG CAG^CTG
3203 CAGCTG
RcaI 2133 TCATGA T^CATGA
2306 TCATGA
ScaI 1382 AGTACT AGT^ACT
1978 AGTACT
SfuI 1973 TTCGAA TT^CGAA
2471 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BanII BbvI BclI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII XmnI