(Circular) (Six-base) MAPSORT of: pFN21AA0389.seq Check: 4065 from: 1 to: 8560 pFN21AA0389 8560 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6370 278 Size: 53 83 186 936 4834 2468 Fragments arranged by size: 4834 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5903 1937 Size: 3966 4594 Acc65I G'GTAC_C Cuts at: 3229 5881 3229 Size: 2652 5908 AclI AA'CG_TT Cuts at: 1563 6687 7060 1563 Size: 5124 373 3063 Fragments arranged by size: 5124 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2313 2793 3063 3116 3491 4055 4467 4974 Size: 681 480 270 53 375 564 412 507 Cuts at: 4974 5774 6623 8007 8275 1632 Size: 800 849 1384 268 1917 Fragments arranged by size: 1917 1384 849 800 681 564 507 480 412 375 270 268 53 AflII C'TTAA_G Cuts at: 820 1017 2906 820 Size: 197 1889 6474 Fragments arranged by size: 6474 1889 197 AflIII A'CryG_T Cuts at: 1203 4152 7459 8436 1203 Size: 2949 3307 977 1327 Fragments arranged by size: 3307 2949 1327 977 AgeI A'CCGG_T Cuts at: 7391 7391 Size: 8560 AhdI GACnn_n'nnGTC Cuts at: 7289 7289 Size: 8560 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4478 1184 Size: 3294 5266 AlwNI CAG_nnn'CTG Cuts at: 4380 5213 5851 6303 7875 4380 Size: 833 638 452 1572 5065 Fragments arranged by size: 5065 1572 833 638 452 ApaLI G'TGCA_C Cuts at: 2965 6617 7773 2965 Size: 3652 1156 3752 Fragments arranged by size: 3752 3652 1156 ApoI r'AATT_y Cuts at: 1493 2259 2489 2627 3958 4056 4298 4559 4629 Size: 766 230 138 1331 98 242 261 70 Cuts at: 4629 5168 5315 5869 6095 6247 8356 1493 Size: 539 147 554 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 766 554 539 261 242 230 226 152 147 138 98 70 AseI AT'TA_AT Cuts at: 160 1032 7114 160 Size: 872 6082 1606 Fragments arranged by size: 6082 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8560 AvaI C'yCGr_G Cuts at: 1870 1943 4598 5885 1870 Size: 73 2655 1287 4545 Fragments arranged by size: 4545 2655 1287 73 BamHI G'GATC_C Cuts at: 4192 5890 6257 4192 Size: 1698 367 6495 Fragments arranged by size: 6495 1698 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 3206 3229 5881 7336 Size: 325 217 627 242 1177 23 2652 1455 Cuts at: 7336 618 Size: 1842 Fragments arranged by size: 2652 1842 1455 1177 627 325 242 217 23 BanII G_rGCy'C Cuts at: 1391 2660 5073 5295 5879 1391 Size: 1269 2413 222 584 4072 Fragments arranged by size: 4072 2413 1269 584 222 BbeI G_GCGC'C Cuts at: 2033 2033 Size: 8560 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2597 4836 928 Size: 971 698 2239 4652 Fragments arranged by size: 4652 2239 971 698 BbvCI CC'TCA_GC Cuts at: 4781 5445 4781 Size: 664 7896 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6751 6785 1903 Size: 34 4814 34 3678 Fragments arranged by size: 4814 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6452 7662 6452 Size: 1210 7350 BclI T'GATC_A Cuts at: 1548 3301 3622 4303 1548 Size: 1753 321 681 5805 Fragments arranged by size: 5805 1753 681 321 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5099 7171 136 Size: 107 122 71 631 605 3427 2072 1525 Fragments arranged by size: 3427 2072 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5733 8555 5733 Size: 2822 5738 BlpI GC'TnA_GC Cuts at: 5977 5977 Size: 8560 Bme1580I G_kGCm'C Cuts at: 1790 2969 3209 4538 6621 7777 1790 Size: 1179 240 1329 2083 1156 2573 Fragments arranged by size: 2573 2083 1329 1179 1156 240 BmgBI CAC'GTC Cuts at: 1318 4823 1318 Size: 3505 5055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3149 3772 6291 Size: 416 53 83 399 24 1732 623 2519 Cuts at: 6291 7249 442 Size: 958 1753 Fragments arranged by size: 2519 1753 1732 958 623 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8560 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2475 4008 5033 7220 1239 Size: 1236 1533 1025 2187 2579 Fragments arranged by size: 2579 2187 1533 1236 1025 Bpu10I CC'TnA_GC Cuts at: 4781 5445 4781 Size: 664 7896 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2411 4898 5717 6685 7550 7848 2411 Size: 2487 819 968 865 298 3123 Fragments arranged by size: 3123 2487 968 865 819 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7223 882 Size: 617 5724 2219 Fragments arranged by size: 5724 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5261 6351 493 Size: 711 4057 1090 2702 Fragments arranged by size: 4057 2702 1090 711 BsaBI GATnn'nnATC Cuts at: 8554 8554 Size: 8560 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 3033 6367 6749 Size: 53 83 186 936 497 1003 3334 382 Cuts at: 6749 7384 275 Size: 635 1451 Fragments arranged by size: 3334 1451 1003 936 635 497 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5140 6993 7391 7665 7812 8223 5140 Size: 1853 398 274 147 411 5477 Fragments arranged by size: 5477 1853 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2661 2673 2691 2703 1178 Size: 30 165 30 1258 12 18 12 7035 Fragments arranged by size: 7035 1258 165 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4510 4721 4871 5000 5714 1186 Size: 3324 211 150 129 714 4032 Fragments arranged by size: 4032 3324 714 211 150 129 BseYI C'CCAG_C Cuts at: 3612 5455 7763 8259 3612 Size: 1843 2308 496 3913 Fragments arranged by size: 3913 2308 1843 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2821 3023 1163 Size: 573 1085 202 6700 Fragments arranged by size: 6700 1085 573 202 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6010 6771 7799 1540 Size: 275 186 4009 761 1028 2301 Fragments arranged by size: 4009 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2427 2660 2969 4054 4403 5073 5295 5395 6621 Size: 233 309 1085 349 670 222 100 1226 Cuts at: 6621 6706 7777 2427 Size: 85 1071 3210 Fragments arranged by size: 3210 1226 1085 1071 670 349 309 233 222 100 85 BsmI GAATG_Cn' Cuts at: 4944 5800 6071 6164 4944 Size: 856 271 93 7340 Fragments arranged by size: 7340 856 271 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3714 3714 Size: 8560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2427 2660 2969 3209 4054 4403 4538 Size: 399 637 233 309 240 845 349 135 Cuts at: 4538 5073 5295 5395 5879 6621 6706 7777 1391 Size: 535 222 100 484 742 85 1071 2174 Fragments arranged by size: 2174 1071 845 742 637 535 484 399 349 309 240 233 222 135 100 85 BspEI T'CCGG_A Cuts at: 8223 8223 Size: 8560 BspHI T'CATG_A Cuts at: 4138 4311 6449 8179 4138 Size: 173 2138 1730 4519 Fragments arranged by size: 4519 2138 1730 173 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2903 5915 844 Size: 2059 3012 3489 Fragments arranged by size: 3489 3012 2059 BsrBI CCG'CTC Cuts at: 1394 1823 6447 1394 Size: 429 4624 3507 Fragments arranged by size: 4624 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3439 7055 7229 65 Size: 1183 2191 3616 174 1396 Fragments arranged by size: 3616 2191 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2468 7204 7391 1700 Size: 110 658 4736 187 2869 Fragments arranged by size: 4736 2869 658 187 110 BsrGI T'GTAC_A Cuts at: 96 4155 96 Size: 4059 4501 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8560 BssSI C'ACGA_G Cuts at: 3261 4125 6620 7632 3261 Size: 864 2495 1012 4189 Fragments arranged by size: 4189 2495 1012 864 BstAPI GCAn_nnn'nTGC Cuts at: 794 3257 794 Size: 2463 6097 BstBI TT'CG_AA Cuts at: 3979 4477 7364 3979 Size: 498 2887 5175 Fragments arranged by size: 5175 2887 498 BstEII G'GTnAC_C Cuts at: 1184 4115 1184 Size: 2931 5629 BstXI CCAn_nnnn'nTGG Cuts at: 3571 5597 3571 Size: 2026 6534 BstYI r'GATC_y Cuts at: 1972 2706 2926 4192 5733 5890 6257 6642 6659 Size: 734 220 1266 1541 157 367 385 17 Cuts at: 6659 8111 8197 8209 8555 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1541 1452 1266 734 385 367 346 220 157 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5045 5045 Size: 8560 BtgI C'CryG_G Cuts at: 513 1065 2004 2944 5603 513 Size: 552 939 940 2659 3470 Fragments arranged by size: 3470 2659 940 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7821 Fragments arranged by size: 7821 683 56 BtsI GCAGTG_nn' Cuts at: 2219 2298 3765 6076 6870 6890 2219 Size: 79 1467 2311 794 20 3889 Fragments arranged by size: 3889 2311 1467 794 79 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8525 DraI TTT'AAA Cuts at: 2208 2826 4222 5864 2208 Size: 618 1396 1642 4904 Fragments arranged by size: 4904 1642 1396 618 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7567 809 Size: 507 6251 1802 Fragments arranged by size: 6251 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4650 5151 6007 Size: 54 1425 50 275 18 2820 501 856 Cuts at: 6007 6896 8525 8 Size: 889 1629 43 Fragments arranged by size: 2820 1629 1425 889 856 501 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6007 1537 Size: 275 4195 4090 Fragments arranged by size: 4195 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 3020 4758 4848 4905 5360 6490 1353 Size: 801 866 1738 90 57 455 1130 3423 Fragments arranged by size: 3423 1738 1130 866 801 455 90 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4882 5522 7147 7521 7667 387 Size: 1064 138 3293 640 1625 374 146 1280 Fragments arranged by size: 3293 1625 1280 1064 640 374 146 138 EcoICRI GAG'CTC Cuts at: 2658 5071 5293 2658 Size: 2413 222 5925 Fragments arranged by size: 5925 2413 222 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2313 2475 2793 3063 3116 3491 4008 Size: 393 681 162 318 270 53 375 517 Cuts at: 4008 4055 4467 4974 5033 5774 6623 7220 8007 Size: 47 412 507 59 741 849 597 787 Cuts at: 8007 8275 1239 Size: 268 1524 Fragments arranged by size: 1524 849 787 741 681 597 517 507 412 393 375 318 270 268 162 59 53 47 EcoO109I rG'GnC_Cy Cuts at: 5183 5183 Size: 8560 EcoRI G'AATT_C Cuts at: 5869 6247 5869 Size: 378 8182 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3023 3176 3023 Size: 153 8407 FspI TGC'GCA Cuts at: 7066 7066 Size: 8560 HaeII r_GCGC'y Cuts at: 1678 2033 5105 7707 1678 Size: 355 3072 2602 2531 Fragments arranged by size: 3072 2602 2531 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4606 4638 4976 5008 5167 Size: 32 135 32 3034 32 338 32 159 Cuts at: 5167 5199 7201 7233 7275 7307 1373 Size: 32 2002 32 42 32 2626 Fragments arranged by size: 3034 2626 2002 338 159 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4263 5904 6150 669 Size: 988 281 2325 1641 246 3079 Fragments arranged by size: 3079 2325 1641 988 281 246 HindIII A'AGCT_T Cuts at: 748 2403 3005 3994 4224 748 Size: 1655 602 989 230 5084 Fragments arranged by size: 5084 1655 989 602 230 HpaI GTT'AAC Cuts at: 4263 6150 4263 Size: 1887 6673 KasI G'GCGC_C Cuts at: 2029 2029 Size: 8560 KpnI G_GTAC'C Cuts at: 3233 5885 3233 Size: 2652 5908 MfeI C'AATT_G Cuts at: 2094 2391 6159 2094 Size: 297 3768 4495 Fragments arranged by size: 4495 3768 297 MluI A'CGCG_T Cuts at: 8436 8436 Size: 8560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2647 7674 7858 1305 Size: 1342 5027 184 2007 Fragments arranged by size: 5027 2007 1342 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4652 5153 10 Size: 54 1425 343 2820 501 3417 Fragments arranged by size: 3417 2820 1425 501 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2420 2646 2972 3929 4460 4670 5595 Size: 648 1254 226 326 957 531 210 925 Cuts at: 5595 5678 6518 6877 7036 518 Size: 83 840 359 159 2042 Fragments arranged by size: 2042 1254 957 925 840 648 531 359 326 226 210 159 83 MspA1I CmG'CkG Cuts at: 1606 4969 5210 5482 5976 6653 7801 8046 1606 Size: 3363 241 272 494 677 1148 245 2120 Fragments arranged by size: 3363 2120 1148 677 494 272 245 241 NaeI GCC'GGC Cuts at: 2470 2470 Size: 8560 NarI GG'CG_CC Cuts at: 2030 2030 Size: 8560 NcoI C'CATG_G Cuts at: 513 1065 2004 2944 5603 513 Size: 552 939 940 2659 3470 Fragments arranged by size: 3470 2659 940 939 552 NdeI CA'TA_TG Cuts at: 387 2269 3810 387 Size: 1882 1541 5137 Fragments arranged by size: 5137 1882 1541 NgoMIV G'CCGG_C Cuts at: 2468 2468 Size: 8560 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8560 NotI GC'GGCC_GC Cuts at: 6007 6007 Size: 8560 NspI r_CATG'y Cuts at: 1132 1229 4156 4374 5918 7463 1132 Size: 97 2927 218 1544 1545 2229 Fragments arranged by size: 2927 2229 1545 1544 218 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8560 PciI A'CATG_T Cuts at: 4152 7459 4152 Size: 3307 5253 PflMI CCAn_nnn'nTGG Cuts at: 1108 3784 1108 Size: 2676 5884 PmeI GTTT'AAAC Cuts at: 5864 5864 Size: 8560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3471 3503 6629 6661 8142 8174 3471 Size: 32 3126 32 1481 32 3857 Fragments arranged by size: 3857 3126 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5183 5183 Size: 8560 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8560 PsiI TTA'TAA Cuts at: 3224 6130 3224 Size: 2906 5654 PstI C_TGCA'G Cuts at: 830 2158 3643 5847 5912 830 Size: 1328 1485 2204 65 3478 Fragments arranged by size: 3478 2204 1485 1328 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8560 PvuII CAG'CTG Cuts at: 4969 5210 4969 Size: 241 8319 SacI G_AGCT'C Cuts at: 2660 5073 5295 2660 Size: 2413 222 5925 Fragments arranged by size: 5925 2413 222 SalI G'TCGA_C Cuts at: 1936 5902 1936 Size: 3966 4594 SbfI CC_TGCA'GG Cuts at: 5912 5912 Size: 8560 ScaI AGT'ACT Cuts at: 3389 3985 6808 8520 3389 Size: 596 2823 1712 3429 Fragments arranged by size: 3429 2823 1712 596 SfcI C'TryA_G Cuts at: 826 1045 2040 2154 3639 5843 5908 7043 7724 Size: 219 995 114 1485 2204 65 1135 681 Cuts at: 7724 7915 826 Size: 191 1471 Fragments arranged by size: 2204 1485 1471 1135 995 681 219 191 114 65 SfoI GGC'GCC Cuts at: 2031 2031 Size: 8560 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8560 SmaI CCC'GGG Cuts at: 1872 5887 1872 Size: 4015 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2426 2906 4877 5696 6664 7565 Size: 197 926 483 480 1971 819 968 901 Cuts at: 7565 7827 820 Size: 262 1553 Fragments arranged by size: 1971 1553 968 926 901 819 483 480 262 197 SnaBI TAC'GTA Cuts at: 493 6351 493 Size: 5858 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8560 SphI G_CATG'C Cuts at: 1132 5918 1132 Size: 4786 3774 SspI AAT'ATT Cuts at: 5 52 2065 3052 3125 6484 5 Size: 47 2013 987 73 3359 2081 Fragments arranged by size: 3359 2081 2013 987 73 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8560 StyI C'CwwG_G Cuts at: 513 1065 2004 2944 3043 5603 5999 513 Size: 552 939 940 99 2560 396 3074 Fragments arranged by size: 3074 2560 940 939 552 396 99 SwaI ATTT'AAAT Cuts at: 2208 2208 Size: 8560 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5659 6587 6604 6757 6942 7387 325 Size: 1255 4079 928 17 153 185 445 1498 Fragments arranged by size: 4079 1498 1255 928 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3387 3983 4155 Size: 274 80 33 51 1443 1410 596 172 Cuts at: 4155 5191 5547 6806 8518 96 Size: 1036 356 1259 1712 138 Fragments arranged by size: 1712 1443 1410 1259 1036 596 356 274 172 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2673 2705 5710 5742 8520 8552 2673 Size: 32 3005 32 2778 32 2681 Fragments arranged by size: 3005 2778 2681 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8560 XbaI T'CTAG_A Cuts at: 5896 5896 Size: 8560 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5653 5653 Size: 8560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8560 XmaI C'CCGG_G Cuts at: 1870 5885 1870 Size: 4015 4545 XmnI GAAnn'nnTTC Cuts at: 2790 3441 3465 5316 6689 2790 Size: 651 24 1851 1373 4661 Fragments arranged by size: 4661 1851 1373 651 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6368 276 Size: 53 83 186 936 4834 2468 Fragments arranged by size: 4834 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI