Restriction map for
pFN21AA0389
(Linear) (Six-base) MAPSORT of: KIAA0389.seq Check: 5647 from: 1 to: 3855
KIAA0389.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
Acc65I G'GTAC_C
Cuts at: 0 1224 3855
Size: 1224 2631
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 788 1058 1111 1486 2050 2462 2969
Size: 308 480 270 53 375 564 412 507
Cuts at: 2969 3769 3855
Size: 800 86
Fragments arranged by size:
800 564 507 480 412 375 308 270
86 53
AflII C'TTAA_G
Cuts at: 0 901 3855
Size: 901 2954
AflIII A'CryG_T
Cuts at: 0 2147 3855
Size: 2147 1708
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2473 3855
Size: 2473 1382
AlwNI CAG_nnn'CTG
Cuts at: 0 2375 3208 3846 3855
Size: 2375 833 638 9
Fragments arranged by size:
2375 833 638 9
ApaLI G'TGCA_C
Cuts at: 0 960 3855
Size: 960 2895
ApoI r'AATT_y
Cuts at: 0 254 484 622 1953 2051 2293 2554 2624
Size: 254 230 138 1331 98 242 261 70
Cuts at: 2624 3163 3310 3855
Size: 539 147 545
Fragments arranged by size:
1331 545 539 261 254 242 230 147
138 98 70
AvaI C'yCGr_G
Cuts at: 0 2593 3855
Size: 2593 1262
BamHI G'GATC_C
Cuts at: 0 2187 3855
Size: 2187 1668
BanI G'GyrC_C
Cuts at: 0 24 1201 1224 3855
Size: 24 1177 23 2631
Fragments arranged by size:
2631 1177 24 23
BanII G_rGCy'C
Cuts at: 0 655 3068 3290 3855
Size: 655 2413 222 565
Fragments arranged by size:
2413 655 565 222
BbeI G_GCGC'C
Cuts at: 0 28 3855
Size: 28 3827
BbsI GAAGACnn'nnnn_
Cuts at: 0 592 2831 3855
Size: 592 2239 1024
Fragments arranged by size:
2239 1024 592
BbvCI CC'TCA_GC
Cuts at: 0 2776 3440 3855
Size: 2776 664 415
Fragments arranged by size:
2776 664 415
BclI T'GATC_A
Cuts at: 0 1296 1617 2298 3855
Size: 1296 321 681 1557
Fragments arranged by size:
1557 1296 681 321
BglI GCCn_nnn'nGGC
Cuts at: 0 3094 3855
Size: 3094 761
BglII A'GATC_T
Cuts at: 0 3728 3855
Size: 3728 127
Bme1580I G_kGCm'C
Cuts at: 0 964 1204 2533 3855
Size: 964 240 1329 1322
Fragments arranged by size:
1329 1322 964 240
BmgBI CAC'GTC
Cuts at: 0 2818 3855
Size: 2818 1037
BmrI ACTGGGnnnn_n'
Cuts at: 0 1144 1767 3855
Size: 1144 623 2088
Fragments arranged by size:
2088 1144 623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 2003 3028 3855
Size: 470 1533 1025 827
Fragments arranged by size:
1533 1025 827 470
Bpu10I CC'TnA_GC
Cuts at: 0 2776 3440 3855
Size: 2776 664 415
Fragments arranged by size:
2776 664 415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 2893 3712 3855
Size: 406 2487 819 143
Fragments arranged by size:
2487 819 406 143
BsaAI yAC'GTr
Cuts at: 0 3256 3855
Size: 3256 599
BsaHI Gr'CG_yC
Cuts at: 0 25 1028 3855
Size: 25 1003 2827
Fragments arranged by size:
2827 1003 25
BsaWI w'CCGG_w
Cuts at: 0 3135 3855
Size: 3135 720
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 656 668 686 698 3855
Size: 656 12 18 12 3157
Fragments arranged by size:
3157 656 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2505 2716 2866 2995 3709 3855
Size: 2505 211 150 129 714 146
Fragments arranged by size:
2505 714 211 150 146 129
BseYI C'CCAG_C
Cuts at: 0 1607 3450 3855
Size: 1607 1843 405
Fragments arranged by size:
1843 1607 405
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 816 1018 3855
Size: 816 202 2837
Fragments arranged by size:
2837 816 202
BsiHKAI G_wGCw'C
Cuts at: 0 422 655 964 2049 2398 3068 3290 3390
Size: 422 233 309 1085 349 670 222 100
Cuts at: 3390 3855
Size: 465
Fragments arranged by size:
1085 670 465 422 349 309 233 222
100
BsmI GAATG_Cn'
Cuts at: 0 2939 3795 3855
Size: 2939 856 60
Fragments arranged by size:
2939 856 60
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1709 3855
Size: 1709 2146
Bsp1286I G_dGCh'C
Cuts at: 0 422 655 964 1204 2049 2398 2533 3068
Size: 422 233 309 240 845 349 135 535
Cuts at: 3068 3290 3390 3855
Size: 222 100 465
Fragments arranged by size:
845 535 465 422 349 309 240 233
222 135 100
BspHI T'CATG_A
Cuts at: 0 2133 2306 3855
Size: 2133 173 1549
Fragments arranged by size:
2133 1549 173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 898 3855
Size: 898 2957
BsrDI GCAATG_nn'
Cuts at: 0 1434 3855
Size: 1434 2421
BsrFI r'CCGG_y
Cuts at: 0 463 3855
Size: 463 3392
BsrGI T'GTAC_A
Cuts at: 0 2150 3855
Size: 2150 1705
BssSI C'ACGA_G
Cuts at: 0 1256 2120 3855
Size: 1256 864 1735
Fragments arranged by size:
1735 1256 864
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1252 3855
Size: 1252 2603
BstBI TT'CG_AA
Cuts at: 0 1974 2472 3855
Size: 1974 498 1383
Fragments arranged by size:
1974 1383 498
BstEII G'GTnAC_C
Cuts at: 0 2110 3855
Size: 2110 1745
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1566 3592 3855
Size: 1566 2026 263
Fragments arranged by size:
2026 1566 263
BstYI r'GATC_y
Cuts at: 0 701 921 2187 3728 3855
Size: 701 220 1266 1541 127
Fragments arranged by size:
1541 1266 701 220 127
Bsu36I CC'TnA_GG
Cuts at: 0 3040 3855
Size: 3040 815
BtgI C'CryG_G
Cuts at: 0 939 3598 3855
Size: 939 2659 257
Fragments arranged by size:
2659 939 257
BtsI GCAGTG_nn'
Cuts at: 0 214 293 1760 3855
Size: 214 79 1467 2095
Fragments arranged by size:
2095 1467 214 79
DraI TTT'AAA
Cuts at: 0 203 821 2217 3855
Size: 203 618 1396 1638
Fragments arranged by size:
1638 1396 618 203
EaeI y'GGCC_r
Cuts at: 0 2645 3146 3855
Size: 2645 501 709
Fragments arranged by size:
2645 709 501
EarI CTCTTCn'nnn_
Cuts at: 0 149 1015 2753 2843 2900 3355 3855
Size: 149 866 1738 90 57 455 500
Fragments arranged by size:
1738 866 500 455 149 90 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2877 3517 3855
Size: 2877 640 338
Fragments arranged by size:
2877 640 338
EcoICRI GAG'CTC
Cuts at: 0 653 3066 3288 3855
Size: 653 2413 222 567
Fragments arranged by size:
2413 653 567 222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 470 788 1058 1111 1486 2003 2050
Size: 308 162 318 270 53 375 517 47
Cuts at: 2050 2462 2969 3028 3769 3855
Size: 412 507 59 741 86
Fragments arranged by size:
741 517 507 412 375 318 308 270
162 86 59 53 47
EcoO109I rG'GnC_Cy
Cuts at: 0 3178 3855
Size: 3178 677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1018 1171 3855
Size: 1018 153 2684
Fragments arranged by size:
2684 1018 153
HaeII r_GCGC'y
Cuts at: 0 28 3100 3855
Size: 28 3072 755
Fragments arranged by size:
3072 755 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2601 2633 2971 3003 3162 3194 3855
Size: 2601 32 338 32 159 32 661
Fragments arranged by size:
2601 661 338 159 32 32 32
HincII GTy'rAC
Cuts at: 0 2258 3855
Size: 2258 1597
HindIII A'AGCT_T
Cuts at: 0 398 1000 1989 2219 3855
Size: 398 602 989 230 1636
Fragments arranged by size:
1636 989 602 398 230
HpaI GTT'AAC
Cuts at: 0 2258 3855
Size: 2258 1597
KasI G'GCGC_C
Cuts at: 0 24 3855
Size: 24 3831
KpnI G_GTAC'C
Cuts at: 0 1228 3855
Size: 1228 2627
MfeI C'AATT_G
Cuts at: 0 89 386 3855
Size: 89 297 3469
Fragments arranged by size:
3469 297 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 3855
Size: 642 3213
MscI TGG'CCA
Cuts at: 0 2647 3148 3855
Size: 2647 501 707
Fragments arranged by size:
2647 707 501
MslI CAynn'nnrTG
Cuts at: 0 415 641 967 1924 2455 2665 3590 3673
Size: 415 226 326 957 531 210 925 83
Cuts at: 3673 3855
Size: 182
Fragments arranged by size:
957 925 531 415 326 226 210 182
83
MspA1I CmG'CkG
Cuts at: 0 2964 3205 3477 3855
Size: 2964 241 272 378
Fragments arranged by size:
2964 378 272 241
NaeI GCC'GGC
Cuts at: 0 465 3855
Size: 465 3390
NarI GG'CG_CC
Cuts at: 0 25 3855
Size: 25 3830
NcoI C'CATG_G
Cuts at: 0 939 3598 3855
Size: 939 2659 257
Fragments arranged by size:
2659 939 257
NdeI CA'TA_TG
Cuts at: 0 264 1805 3855
Size: 264 1541 2050
Fragments arranged by size:
2050 1541 264
NgoMIV G'CCGG_C
Cuts at: 0 463 3855
Size: 463 3392
NspI r_CATG'y
Cuts at: 0 2151 2369 3855
Size: 2151 218 1486
Fragments arranged by size:
2151 1486 218
PciI A'CATG_T
Cuts at: 0 2147 3855
Size: 2147 1708
PflMI CCAn_nnn'nTGG
Cuts at: 0 1779 3855
Size: 1779 2076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1466 1498 3855
Size: 1466 32 2357
Fragments arranged by size:
2357 1466 32
PpuMI rG'GwC_Cy
Cuts at: 0 3178 3855
Size: 3178 677
PsiI TTA'TAA
Cuts at: 0 1219 3855
Size: 1219 2636
PstI C_TGCA'G
Cuts at: 0 153 1638 3842 3855
Size: 153 1485 2204 13
Fragments arranged by size:
2204 1485 153 13
PvuII CAG'CTG
Cuts at: 0 2964 3205 3855
Size: 2964 241 650
Fragments arranged by size:
2964 650 241
SacI G_AGCT'C
Cuts at: 0 655 3068 3290 3855
Size: 655 2413 222 565
Fragments arranged by size:
2413 655 565 222
ScaI AGT'ACT
Cuts at: 0 1384 1980 3855
Size: 1384 596 1875
Fragments arranged by size:
1875 1384 596
SfcI C'TryA_G
Cuts at: 0 35 149 1634 3838 3855
Size: 35 114 1485 2204 17
Fragments arranged by size:
2204 1485 114 35 17
SfoI GGC'GCC
Cuts at: 0 26 3855
Size: 26 3829
SmlI C'TyrA_G
Cuts at: 0 421 901 2872 3691 3855
Size: 421 480 1971 819 164
Fragments arranged by size:
1971 819 480 421 164
SspI AAT'ATT
Cuts at: 0 60 1047 1120 3855
Size: 60 987 73 2735
Fragments arranged by size:
2735 987 73 60
StyI C'CwwG_G
Cuts at: 0 939 1038 3598 3855
Size: 939 99 2560 257
Fragments arranged by size:
2560 939 257 99
SwaI ATTT'AAAT
Cuts at: 0 203 3855
Size: 203 3652
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3654 3855
Size: 3654 201
TatI w'GTAC_w
Cuts at: 0 1382 1978 2150 3186 3542 3855
Size: 1382 596 172 1036 356 313
Fragments arranged by size:
1382 1036 596 356 313 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 668 700 3705 3737 3855
Size: 668 32 3005 32 118
Fragments arranged by size:
3005 668 118 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3648 3855
Size: 3648 207
XmnI GAAnn'nnTTC
Cuts at: 0 785 1436 1460 3311 3855
Size: 785 651 24 1851 544
Fragments arranged by size:
1851 785 651 544 24
Enzymes that do cut:
Acc65I AcuI AflII AflIII AlfI AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI
BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PpiI PpuMI PsiI
PstI PvuII SacI ScaI SfcI SfoI SmlI SspI
StyI SwaI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AhdI AleI
AloI ApaI AscI AseI AsiSI AvrII BaeI BcgI
BciVI BfrBI BlpI BmtI BplI BsaI BsaBI BsiEI
BsiWI BspEI BsrBI BssHII BstZ17I BtgZI ClaI CspCI
DraIII DrdI EagI EcoNI EcoRI EcoRV FseI FspI
FspAI MluI NheI NotI NruI NsiI PacI PasI
PfoI PmeI PmlI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
Tth111I XbaI XhoI XmaI ZraI