(Circular) (Six-base) MAPSORT of: pFN21AA0371.seq Check: 1137 from: 1 to: 8299 pFN21AA0371 8299 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2374 5308 2374 Size: 2934 5365 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6109 278 Size: 53 83 186 936 4573 2468 Fragments arranged by size: 4573 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5642 1937 Size: 3705 4594 Acc65I G'GTAC_C Cuts at: 4466 5620 4466 Size: 1154 7145 AclI AA'CG_TT Cuts at: 1563 3418 5598 6426 6799 1563 Size: 1855 2180 828 373 3063 Fragments arranged by size: 3063 2180 1855 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2078 2357 2886 3446 4641 5180 5322 6362 Size: 446 279 529 560 1195 539 142 1040 Cuts at: 6362 7746 8014 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1195 1040 560 539 529 446 279 268 142 AfeI AGC'GCT Cuts at: 3189 3189 Size: 8299 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8102 AflIII A'CryG_T Cuts at: 1203 3602 4545 4736 7198 8175 1203 Size: 2399 943 191 2462 977 1327 Fragments arranged by size: 2462 2399 1327 977 943 191 AgeI A'CCGG_T Cuts at: 3378 7130 3378 Size: 3752 4547 AhdI GACnn_n'nnGTC Cuts at: 7028 7028 Size: 8299 AleI CACnn'nnGTG Cuts at: 1166 3758 4016 1166 Size: 2592 258 5449 Fragments arranged by size: 5449 2592 258 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3125 1184 Size: 1941 6358 AlwNI CAG_nnn'CTG Cuts at: 2646 2972 3088 4805 5095 5158 5185 6042 7614 Size: 326 116 1717 290 63 27 857 1572 Cuts at: 7614 2646 Size: 3331 Fragments arranged by size: 3331 1717 1572 857 326 290 116 63 27 ApaI G_GGCC'C Cuts at: 4584 4584 Size: 8299 ApaLI G'TGCA_C Cuts at: 3100 3774 6356 7512 3100 Size: 674 2582 1156 3887 Fragments arranged by size: 3887 2582 1156 674 ApoI r'AATT_y Cuts at: 1493 3368 5608 5834 5986 8095 1493 Size: 1875 2240 226 152 2109 1697 Fragments arranged by size: 2240 2109 1875 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6853 160 Size: 872 5821 1606 Fragments arranged by size: 5821 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8299 AvaI C'yCGr_G Cuts at: 1870 1943 2838 5624 1870 Size: 73 895 2786 4545 Fragments arranged by size: 4545 2786 895 73 AvrII C'CTAG_G Cuts at: 4000 4000 Size: 8299 BamHI G'GATC_C Cuts at: 3979 5629 5996 3979 Size: 1650 367 6282 Fragments arranged by size: 6282 1650 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2676 3794 4028 4466 4793 Size: 325 217 627 889 1118 234 438 327 Cuts at: 4793 5620 7075 618 Size: 827 1455 1842 Fragments arranged by size: 1842 1455 1118 889 827 627 438 327 325 234 217 BanII G_rGCy'C Cuts at: 1391 2589 4320 4584 5618 1391 Size: 1198 1731 264 1034 4072 Fragments arranged by size: 4072 1731 1198 1034 264 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4199 928 Size: 971 2300 5028 Fragments arranged by size: 5028 2300 971 BbvCI CC'TCA_GC Cuts at: 4059 4207 4059 Size: 148 8151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3755 3789 6490 6524 1903 Size: 34 1818 34 2701 34 3678 Fragments arranged by size: 3678 2701 1818 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6191 7401 6191 Size: 1210 7089 BclI T'GATC_A Cuts at: 1548 1548 Size: 8299 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3966 4130 6910 Size: 107 122 71 631 605 2294 164 2780 Cuts at: 6910 136 Size: 1525 Fragments arranged by size: 2780 2294 1525 631 605 164 122 107 71 BglII A'GATC_T Cuts at: 2052 2380 4598 8294 2052 Size: 328 2218 3696 2057 Fragments arranged by size: 3696 2218 2057 328 BlpI GC'TnA_GC Cuts at: 3285 3953 5716 3285 Size: 668 1763 5868 Fragments arranged by size: 5868 1763 668 Bme1580I G_kGCm'C Cuts at: 1790 3104 3778 4584 4798 6360 7516 1790 Size: 1314 674 806 214 1562 1156 2573 Fragments arranged by size: 2573 1562 1314 1156 806 674 214 BmgBI CAC'GTC Cuts at: 1318 2872 1318 Size: 1554 6745 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3419 4247 4436 Size: 416 53 83 399 24 2002 828 189 Cuts at: 4436 5499 6030 6988 442 Size: 1063 531 958 1753 Fragments arranged by size: 2002 1753 1063 958 828 531 416 399 189 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8299 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4296 5314 4296 Size: 1018 7281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2551 3993 4325 4391 4832 5192 5437 5536 Size: 1312 1442 332 66 441 360 245 99 Cuts at: 5536 6959 1239 Size: 1423 2579 Fragments arranged by size: 2579 1442 1423 1312 441 360 332 245 99 66 Bpu10I CC'TnA_GC Cuts at: 3818 4059 4207 3818 Size: 241 148 7910 Fragments arranged by size: 7910 241 148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2051 2565 4945 6424 7289 7587 2051 Size: 514 2380 1479 865 298 2763 Fragments arranged by size: 2763 2380 1479 865 514 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2442 2853 4505 6962 882 Size: 617 943 411 1652 2457 2219 Fragments arranged by size: 2457 2219 1652 943 617 411 BsaAI yAC'GTr Cuts at: 493 1204 6090 493 Size: 711 4886 2702 Fragments arranged by size: 4886 2702 711 BsaBI GATnn'nnATC Cuts at: 2088 2757 3216 8293 2088 Size: 669 459 5077 2094 Fragments arranged by size: 5077 2094 669 459 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6106 6488 7123 275 Size: 53 83 186 936 4573 382 635 1451 Fragments arranged by size: 4573 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3378 6732 7130 7404 7551 7962 3378 Size: 3354 398 274 147 411 3715 Fragments arranged by size: 3715 3354 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5165 5195 1178 Size: 30 165 30 3762 30 4282 Fragments arranged by size: 4282 3762 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3009 3088 4115 4270 4319 1186 Size: 1823 79 1027 155 49 5166 Fragments arranged by size: 5166 1823 1027 155 79 49 BseYI C'CCAG_C Cuts at: 3252 3784 3908 4649 5310 5483 7502 7998 3252 Size: 532 124 741 661 173 2019 496 3553 Fragments arranged by size: 3553 2019 741 661 532 496 173 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2476 3539 3744 1163 Size: 573 740 1063 205 5718 Fragments arranged by size: 5718 1063 740 573 205 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2804 3227 5749 6510 7538 1540 Size: 275 186 803 423 2522 761 1028 2301 Fragments arranged by size: 2522 2301 1028 803 761 423 275 186 BsiHKAI G_wGCw'C Cuts at: 2129 2589 3104 3778 3936 6360 6445 7516 2129 Size: 460 515 674 158 2424 85 1071 2912 Fragments arranged by size: 2912 2424 1071 674 515 460 158 85 BsmI GAATG_Cn' Cuts at: 2249 3011 3500 5810 5903 2249 Size: 762 489 2310 93 4645 Fragments arranged by size: 4645 2310 762 489 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2589 3104 3778 3936 4320 4584 Size: 399 339 460 515 674 158 384 264 Cuts at: 4584 4798 5618 6360 6445 7516 1391 Size: 214 820 742 85 1071 2174 Fragments arranged by size: 2174 1071 820 742 674 515 460 399 384 339 264 214 158 85 BspEI T'CCGG_A Cuts at: 7962 7962 Size: 8299 BspHI T'CATG_A Cuts at: 6188 7918 6188 Size: 1730 6569 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2374 3741 4129 5308 5654 844 Size: 1530 1367 388 1179 346 3489 Fragments arranged by size: 3489 1530 1367 1179 388 346 BsrBI CCG'CTC Cuts at: 1394 1823 6186 1394 Size: 429 4363 3507 Fragments arranged by size: 4363 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2160 2692 2697 4795 4873 5580 6794 Size: 1183 912 532 5 2098 78 707 1214 Cuts at: 6794 6968 65 Size: 174 1396 Fragments arranged by size: 2098 1396 1214 1183 912 707 532 174 78 5 BsrFI r'CCGG_y Cuts at: 1700 1810 3226 3378 3822 3891 3936 6943 7130 Size: 110 1416 152 444 69 45 3007 187 Cuts at: 7130 1700 Size: 2869 Fragments arranged by size: 3007 2869 1416 444 187 152 110 69 45 BsrGI T'GTAC_A Cuts at: 96 5070 96 Size: 4974 3325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8299 BssSI C'ACGA_G Cuts at: 3335 6359 7371 3335 Size: 3024 1012 4263 Fragments arranged by size: 4263 3024 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2283 2402 5523 794 Size: 1489 119 3121 3570 Fragments arranged by size: 3570 3121 1489 119 BstBI TT'CG_AA Cuts at: 4393 7103 4393 Size: 2710 5589 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8299 BstXI CCAn_nnnn'nTGG Cuts at: 2687 3760 2687 Size: 1073 7226 BstYI r'GATC_y Cuts at: 1972 2052 2380 3115 3979 4598 5629 5996 6381 Size: 80 328 735 864 619 1031 367 385 Cuts at: 6381 6398 7850 7936 7948 8294 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1031 864 735 619 385 367 346 328 86 80 17 12 Bsu36I CC'TnA_GG Cuts at: 4200 4200 Size: 8299 BtgI C'CryG_G Cuts at: 513 1065 2004 4641 513 Size: 552 939 2637 4171 Fragments arranged by size: 4171 2637 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7560 Fragments arranged by size: 7560 683 56 BtsI GCAGTG_nn' Cuts at: 2327 3238 3443 3732 5374 5422 5815 6609 6629 Size: 911 205 289 1642 48 393 794 20 Cuts at: 6629 2327 Size: 3997 Fragments arranged by size: 3997 1642 911 794 393 289 205 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8299 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2548 2583 529 Size: 35 1984 35 6245 Fragments arranged by size: 6245 1984 35 35 DraI TTT'AAA Cuts at: 2488 5603 2488 Size: 3115 5184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7306 809 Size: 507 5990 1802 Fragments arranged by size: 5990 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3224 3361 3885 Size: 54 1425 50 275 18 1394 137 524 Cuts at: 3885 5367 5746 6635 8264 8 Size: 1482 379 889 1629 43 Fragments arranged by size: 1629 1482 1425 1394 889 524 379 275 137 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3224 5746 1537 Size: 275 1412 2522 4090 Fragments arranged by size: 4090 2522 1412 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2007 2333 2390 4395 5156 6229 1353 Size: 654 326 57 2005 761 1073 3423 Fragments arranged by size: 3423 2005 1073 761 654 326 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6886 7260 7406 387 Size: 1064 138 5297 374 146 1280 Fragments arranged by size: 5297 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2587 2587 Size: 8299 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2357 2551 2886 3446 3993 4325 Size: 393 446 279 194 335 560 547 332 Cuts at: 4325 4391 4641 4832 5180 5192 5322 5437 5536 Size: 66 250 191 348 12 130 115 99 Cuts at: 5536 6362 6959 7746 8014 1239 Size: 826 597 787 268 1524 Fragments arranged by size: 1524 826 787 597 560 547 446 393 348 335 332 279 268 250 194 191 130 115 99 66 12 EcoO109I rG'GnC_Cy Cuts at: 2643 3331 4162 4345 4375 4581 4680 4815 2643 Size: 688 831 183 30 206 99 135 6127 Fragments arranged by size: 6127 831 688 206 183 135 99 30 EcoRI G'AATT_C Cuts at: 5608 5986 5608 Size: 378 7921 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3521 4448 4796 3521 Size: 927 348 7024 Fragments arranged by size: 7024 927 348 FspI TGC'GCA Cuts at: 6805 6805 Size: 8299 HaeII r_GCGC'y Cuts at: 1678 3191 3898 3943 5095 7446 1678 Size: 1513 707 45 1152 2351 2531 Fragments arranged by size: 2531 2351 1513 1152 707 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2172 2204 2372 2404 2786 Size: 32 135 32 600 32 168 32 382 Cuts at: 2786 2818 2942 2974 3100 3132 4246 4278 5048 Size: 32 124 32 126 32 1114 32 770 Cuts at: 5048 5080 6940 6972 7014 7046 1373 Size: 32 1860 32 42 32 2626 Fragments arranged by size: 2626 1860 1114 770 600 382 168 135 126 124 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2823 5643 5889 669 Size: 988 281 885 2820 246 3079 Fragments arranged by size: 3079 2820 988 885 281 246 HindIII A'AGCT_T Cuts at: 748 2781 2936 748 Size: 2033 155 6111 Fragments arranged by size: 6111 2033 155 HpaI GTT'AAC Cuts at: 5889 5889 Size: 8299 KpnI G_GTAC'C Cuts at: 4470 5624 4470 Size: 1154 7145 MfeI C'AATT_G Cuts at: 5898 5898 Size: 8299 MluI A'CGCG_T Cuts at: 8175 8175 Size: 8299 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2383 2565 2950 5110 5619 7413 7597 1305 Size: 1078 182 385 2160 509 1794 184 2007 Fragments arranged by size: 2160 2007 1794 1078 509 385 184 182 MscI TGG'CCA Cuts at: 10 64 1489 1832 3363 3887 10 Size: 54 1425 343 1531 524 4422 Fragments arranged by size: 4422 1531 1425 524 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3107 3553 3758 4016 4477 4550 5066 Size: 648 1941 446 205 258 461 73 516 Cuts at: 5066 5205 5339 5379 6257 6616 6775 518 Size: 139 134 40 878 359 159 2042 Fragments arranged by size: 2042 1941 878 648 516 461 446 359 258 205 159 139 134 73 40 MspA1I CmG'CkG Cuts at: 1606 2071 2706 2969 3223 3771 4924 5310 5715 Size: 465 635 263 254 548 1153 386 405 Cuts at: 5715 6392 7540 7785 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1153 1148 677 635 548 465 405 386 263 254 245 NaeI GCC'GGC Cuts at: 3824 3893 3824 Size: 69 8230 NcoI C'CATG_G Cuts at: 513 1065 2004 4641 513 Size: 552 939 2637 4171 Fragments arranged by size: 4171 2637 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8299 NgoMIV G'CCGG_C Cuts at: 3822 3891 3822 Size: 69 8230 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8299 NotI GC'GGCC_GC Cuts at: 5746 5746 Size: 8299 NspI r_CATG'y Cuts at: 1132 1229 2474 3206 3606 4549 5065 5657 7202 Size: 97 1245 732 400 943 516 592 1545 Cuts at: 7202 1132 Size: 2229 Fragments arranged by size: 2229 1545 1245 943 732 592 516 400 97 PasI CC'CwG_GG Cuts at: 1348 4353 1348 Size: 3005 5294 PciI A'CATG_T Cuts at: 3602 4545 7198 3602 Size: 943 2653 4703 Fragments arranged by size: 4703 2653 943 PflMI CCAn_nnn'nTGG Cuts at: 1108 3274 1108 Size: 2166 6133 PfoI T'CCnGG_A Cuts at: 2076 2645 3118 5151 2076 Size: 569 473 2033 5224 Fragments arranged by size: 5224 2033 569 473 PmeI GTTT'AAAC Cuts at: 5603 5603 Size: 8299 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6368 6400 7881 7913 6368 Size: 32 1481 32 6754 Fragments arranged by size: 6754 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2643 3331 4162 4345 4375 2643 Size: 688 831 183 30 6567 Fragments arranged by size: 6567 831 688 183 30 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8299 PsiI TTA'TAA Cuts at: 5869 5869 Size: 8299 PspOMI G'GGCC_C Cuts at: 4580 4580 Size: 8299 PstI C_TGCA'G Cuts at: 830 3438 3902 4943 5285 5429 5651 830 Size: 2608 464 1041 342 144 222 3478 Fragments arranged by size: 3478 2608 1041 464 342 222 144 PvuI CG_AT'CG Cuts at: 2001 2804 2001 Size: 803 7496 PvuII CAG'CTG Cuts at: 2071 2969 3771 4924 5310 2071 Size: 898 802 1153 386 5060 Fragments arranged by size: 5060 1153 898 802 386 SacI G_AGCT'C Cuts at: 2589 2589 Size: 8299 SalI G'TCGA_C Cuts at: 1936 5641 1936 Size: 3705 4594 SbfI CC_TGCA'GG Cuts at: 5651 5651 Size: 8299 ScaI AGT'ACT Cuts at: 6547 8259 6547 Size: 1712 6587 SfcI C'TryA_G Cuts at: 826 1045 2671 3434 3898 4539 4939 5281 5425 Size: 219 1626 763 464 641 400 342 144 Cuts at: 5425 5527 5647 6782 7463 7654 826 Size: 102 120 1135 681 191 1471 Fragments arranged by size: 1626 1471 1135 763 681 641 464 400 342 219 191 144 120 102 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8299 SgrAI Cr'CCGG_yG Cuts at: 1700 3936 1700 Size: 2236 6063 SmaI CCC'GGG Cuts at: 1872 5626 1872 Size: 3754 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2030 2580 2838 4960 6403 7304 Size: 197 926 87 550 258 2122 1443 901 Cuts at: 7304 7566 820 Size: 262 1553 Fragments arranged by size: 2122 1553 1443 926 901 550 262 258 197 87 SnaBI TAC'GTA Cuts at: 493 6090 493 Size: 5597 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8299 SphI G_CATG'C Cuts at: 1132 3206 5657 1132 Size: 2074 2451 3774 Fragments arranged by size: 3774 2451 2074 SspI AAT'ATT Cuts at: 5 52 6223 5 Size: 47 6171 2081 Fragments arranged by size: 6171 2081 47 StuI AGG'CCT Cuts at: 1517 4205 1517 Size: 2688 5611 StyI C'CwwG_G Cuts at: 513 1065 2004 3567 4000 4585 4641 4714 5738 Size: 552 939 1563 433 585 56 73 1024 Cuts at: 5738 513 Size: 3074 Fragments arranged by size: 3074 1563 1024 939 585 552 433 73 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4229 4879 6326 6343 6496 6681 7126 Size: 1255 2649 650 1447 17 153 185 445 Cuts at: 7126 325 Size: 1498 Fragments arranged by size: 2649 1498 1447 1255 650 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2556 3235 5070 Size: 274 80 33 51 1443 579 679 1835 Cuts at: 5070 6545 8257 96 Size: 1475 1712 138 Fragments arranged by size: 1835 1712 1475 1443 679 579 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3332 3364 4512 4544 8259 8291 3332 Size: 32 1148 32 3715 32 3340 Fragments arranged by size: 3715 3340 1148 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8299 XbaI T'CTAG_A Cuts at: 5635 5635 Size: 8299 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2724 2724 Size: 8299 XhoI C'TCGA_G Cuts at: 1943 2838 1943 Size: 895 7404 XmaI C'CCGG_G Cuts at: 1870 5624 1870 Size: 3754 4545 XmnI GAAnn'nnTTC Cuts at: 4428 5444 6428 4428 Size: 1016 984 6299 Fragments arranged by size: 6299 1016 984 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6107 276 Size: 53 83 186 936 4573 2468 Fragments arranged by size: 4573 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI