Restriction map for
pFN21AA0370
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# NO CUT ENZYMES ( 84 enzymes) #
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Acc65I AclI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BfrI
BglI BlnI BsaAI BsiWI BsmBI
BsmI BspDI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoT22I FauI FseI FspI
HaeII HindIII KasI KpnI KspI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 353 GACGTC GACGT^C
AccI 3166 GTCGAC GT^[AC][GT]AC
AccII 913 CGCG CG^CG
AflIII 2600 ACATGT A^C[GA][CT]GT
Alw44I 3301 GTGCAC G^TGCAC
AocI 629 CCTCAGG CC^T[ACGT]AGG
ApaLI 3301 GTGCAC G^TGCAC
BbsI 450 GAAGAC GAAGAC(2/6)
BbvCI 2899 GCTGAGG CCTCAGC(-5/-2)
BcgI 2419 CGACAGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2766 GTATCC GTATCC(6/5)
BglII 3047 AGATCT A^GATCT
BlpI 2514 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2514 GCTGAGC GC^T[ACGT]AGC
BpuAI 450 GAAGAC GAAGAC(2/6)
BsiEI 915 CGGCCG CG[GA][CT]^CG
BspHI 1230 TCATGA T^CATGA
BspLU11I 2600 ACATGT A^CATGT
BsrDI 2775 CATTGC GCAATG(2/0)
BssSI 2638 CACGAG CACGAG(-5/-1)
BstUI 913 CGCG CG^CG
Bsu36I 629 CCTCAGG CC^T[ACGT]AGG
CelII 2514 GCTGAGC GC^T[ACGT]AGC
DraI 2986 TTTAAA TTT^AAA
DraIII 1060 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 915 CGGCCG C^GGCCG
EclXI 915 CGGCCG C^GGCCG
Eco52I 915 CGGCCG C^GGCCG
Eco81I 629 CCTCAGG CC^T[ACGT]AGG
EcoRV 1255 GATATC GAT^ATC
HpaI 874 GTTAAC GTT^AAC
MvnI 913 CGCG CG^CG
NcoI 1970 CCATGG C^CATGG
RcaI 1230 TCATGA T^CATGA
SalI 3166 GTCGAC G^TCGAC
SbfI 2685 CCTGCAGG CCTGCA^GG
ScaI 1706 AGTACT AGT^ACT
SexAI 533 ACCAGGT A^CC[AT]GGT
SmaI 2276 CCCGGG CCC^GGG
SmlI 1668 CTTGAG C^T[CT][GA]AG
SphI 1860 GCATGC GCATG^C
Sse8387I 2685 CCTGCAGG CCTGCA^GG
StuI 2541 AGGCCT AGG^CCT
XmaCI 2276 CCCGGG C^CCGGG
XmaI 2276 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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AcyI 353 GACGTC G[GA]^CG[CT]C
867 GGCGTC
BfaI 164 CTAG C^TAG
1078 CTAG
BsaHI 353 GACGTC G[GA]^CG[CT]C
867 GGCGTC
BsaI 2043 GGTCTC GGTCTC(1/5)
2457 GGTCTC
BstAPI 540 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2648 GCACAGGCTGC
Hin1I 353 GACGTC G[GA]^CG[CT]C
867 GGCGTC
MaeI 164 CTAG C^TAG
1078 CTAG
NaeI 917 GCCGGC GCC^GGC
1640 GCCGGC
NgoMIV 917 GCCGGC G^CCGGC
1640 GCCGGC
NspI 1860 GCATGC [GA]CATG^[CT]
2600 ACATGT
PvuII 1405 CAGCTG CAG^CTG
3213 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanII BbvI
BclI BcnI BmyI BpmI BsaBI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspEI BspMI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI