Restriction map for
pFN21AA0361
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# NO CUT ENZYMES ( 74 enzymes) #
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AatII Acc65I AflII AgeI AscI
AseI AsnI Asp718I AspI AvrII
BamHI BcgI BfrI BglII BlnI
BsaBI BsiWI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI HpaI KpnI
MamI MluI MseI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1366 GTCTAC GT^[AC][GT]AC
AclI 1745 AACGTT AA^CGTT
AflIII 906 ACATGT A^C[GA][CT]GT
AhdI 905 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1914 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 905 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3693 TGCGCA TGC^GCA
BbeI 3670 GGCGCC GGCGC^C
BbsI 913 GTCTTC GAAGAC(2/6)
BciVI 1110 GTATCC GTATCC(6/5)
BfaI 598 CTAG C^TAG
BpuAI 913 GTCTTC GAAGAC(2/6)
BsmBI 3037 GAGACG CGTCTC(1/5)
BsmI 1647 GAATGC GAATGC(1/-1)
BspLU11I 906 ACATGT A^CATGT
BssSI 2854 CTCGTG CACGAG(-5/-1)
BstYI 1095 GGATCT [GA]^GATC[CT]
DraIII 1024 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2203 GACCCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 905 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1691 CTCTTC CTCTTC(1/4)
FspI 3693 TGCGCA TGC^GCA
HgaI 104 GCGTC GACGC(5/10)
HincII 3482 GTCAAC GT[CT]^[GA]AC
HindII 3482 GTCAAC GT[CT]^[GA]AC
HindIII 1101 AAGCTT A^AGCTT
KasI 3670 GGCGCC G^GCGCC
Ksp632I 1691 CTCTTC CTCTTC(1/4)
KspI 1014 CCGCGG CCGC^GG
MaeI 598 CTAG C^TAG
MfeI 3305 CAATTG C^AATTG
MflI 1095 GGATCT [GA]^GATC[CT]
MunI 3305 CAATTG C^AATTG
NarI 3670 GGCGCC GG^CGCC
PshAI 1725 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1745 AACGTT AA^CGTT
PvuI 1806 CGATCG CGAT^CG
SacII 1014 CCGCGG CCGC^GG
SapI 1690 GCTCTTC GCTCTTC(1/4)
SexAI 2249 ACCTGGT A^CC[AT]GGT
SfoI 3670 GGCGCC GGC^GCC
SmlI 2524 CTCAAG C^T[CT][GA]AG
SnaBI 3709 TACGTA TAC^GTA
SphI 494 GCATGC GCATG^C
XhoII 1095 GGATCT [GA]^GATC[CT]
XmnI 1914 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 37 enzymes) #
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AccII 325 CGCG CG^CG
1015 CGCG
AccIII 3437 TCCGGA T^CCGGA
3726 TCCGGA
AcsI 802 AAATTC [GA]^AATT[CT]
1119 GAATTT
AcyI 103 GGCGTC G[GA]^CG[CT]C
3670 GGCGCC
AfaI 1674 GTAC GT^AC
3807 GTAC
AocI 856 CCTGAGG CC^T[ACGT]AGG
3102 CCTGAGG
Aor51HI 614 AGCGCT AGC^GCT
2030 AGCGCT
ApoI 802 AAATTC [GA]^AATT[CT]
1119 GAATTT
BanI 1768 GGCACC G^G[CT][GA]CC
3670 GGCGCC
BbrPI 718 CACGTG CAC^GTG
1024 CACGTG
BclI 1493 TGATCA T^GATCA
1545 TGATCA
BlpI 1974 GCTGAGC GC^T[ACGT]AGC
3421 GCTGAGC
Bpu1102I 1974 GCTGAGC GC^T[ACGT]AGC
3421 GCTGAGC
BsaHI 103 GGCGTC G[GA]^CG[CT]C
3670 GGCGCC
BseAI 3437 TCCGGA T^CCGGA
3726 TCCGGA
BsiEI 438 CGACCG CG[GA][CT]^CG
1806 CGATCG
BspEI 3437 TCCGGA T^CCGGA
3726 TCCGGA
BspHI 761 TCATGA T^CATGA
1542 TCATGA
BspMI 254 ACCTGC ACCTGC(4/8)
1407 GCAGGT
BstUI 325 CGCG CG^CG
1015 CGCG
Bsu36I 856 CCTGAGG CC^T[ACGT]AGG
3102 CCTGAGG
CelII 1974 GCTGAGC GC^T[ACGT]AGC
3421 GCTGAGC
Eco47III 614 AGCGCT AGC^GCT
2030 AGCGCT
Eco81I 856 CCTGAGG CC^T[ACGT]AGG
3102 CCTGAGG
EcoNI 2880 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3027 CCTCTGGGAGG
FbaI 1493 TGATCA T^GATCA
1545 TGATCA
Hin1I 103 GGCGTC G[GA]^CG[CT]C
3670 GGCGCC
MroI 3437 TCCGGA T^CCGGA
3726 TCCGGA
MvnI 325 CGCG CG^CG
1015 CGCG
NaeI 539 GCCGGC GCC^GGC
1042 GCCGGC
NcoI 1295 CCATGG C^CATGG
2963 CCATGG
NgoMIV 539 GCCGGC G^CCGGC
1042 GCCGGC
PmaCI 718 CACGTG CAC^GTG
1024 CACGTG
PmlI 718 CACGTG CAC^GTG
1024 CACGTG
RcaI 761 TCATGA T^CATGA
1542 TCATGA
RsaI 1674 GTAC GT^AC
3807 GTAC
SacI 1882 GAGCTC GAGCT^C
2149 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AluI Alw44I AlwI AlwNI
ApaI ApaLI AspHI AvaI AvaII
BalI BanII BbvCI BbvI BcnI
BglI BmyI BpmI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI