(Circular) (Six-base) MAPSORT of: pFN21AA0361.seq Check: 1599 from: 1 to: 8719 pFN21AA0361 8719 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3403 3403 Size: 8719 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6529 278 Size: 53 83 186 936 4993 2468 Fragments arranged by size: 4993 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3372 6062 1937 Size: 1435 2690 4594 Fragments arranged by size: 4594 2690 1435 Acc65I G'GTAC_C Cuts at: 6040 6040 Size: 8719 AclI AA'CG_TT Cuts at: 1563 3751 6846 7219 1563 Size: 2188 3095 373 3063 Fragments arranged by size: 3095 3063 2188 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4560 4663 4919 5338 5446 6782 8166 8434 Size: 2928 103 256 419 108 1336 1384 268 Cuts at: 8434 1632 Size: 1917 Fragments arranged by size: 2928 1917 1384 1336 419 268 256 108 103 AfeI AGC'GCT Cuts at: 2621 4037 2621 Size: 1416 7303 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8522 AflIII A'CryG_T Cuts at: 1203 2911 7618 8595 1203 Size: 1708 4707 977 1327 Fragments arranged by size: 4707 1708 1327 977 AgeI A'CCGG_T Cuts at: 7550 7550 Size: 8719 AhdI GACnn_n'nnGTC Cuts at: 2915 7448 2915 Size: 4533 4186 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8719 AlwNI CAG_nnn'CTG Cuts at: 2060 2740 3961 3997 4514 4696 4897 5399 6462 Size: 680 1221 36 517 182 201 502 1063 Cuts at: 6462 8034 2060 Size: 1572 2745 Fragments arranged by size: 2745 1572 1221 1063 680 517 502 201 182 36 ApaI G_GGCC'C Cuts at: 2280 3028 4104 4962 5127 5552 2280 Size: 748 1076 858 165 425 5447 Fragments arranged by size: 5447 1076 858 748 425 165 ApaLI G'TGCA_C Cuts at: 2744 3010 3986 6776 7932 2744 Size: 266 976 2790 1156 3531 Fragments arranged by size: 3531 2790 1156 976 266 ApoI r'AATT_y Cuts at: 1493 2807 3124 6028 6254 6406 8515 1493 Size: 1314 317 2904 226 152 2109 1697 Fragments arranged by size: 2904 2109 1697 1314 317 226 152 AseI AT'TA_AT Cuts at: 160 1032 7273 160 Size: 872 6241 1606 Fragments arranged by size: 6241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8719 AvaI C'yCGr_G Cuts at: 1870 1943 2226 2644 4434 4592 5193 6044 1870 Size: 73 283 418 1790 158 601 851 4545 Fragments arranged by size: 4545 1790 851 601 418 283 158 73 BamHI G'GATC_C Cuts at: 6049 6416 6049 Size: 367 8352 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3773 5675 6040 7495 618 Size: 325 217 627 1986 1902 365 1455 1842 Fragments arranged by size: 1986 1902 1842 1455 627 365 325 217 BanII G_rGCy'C Cuts at: 1391 2280 3028 3891 4104 4158 4462 4577 4962 Size: 889 748 863 213 54 304 115 385 Cuts at: 4962 5032 5127 5378 5552 6038 1391 Size: 70 95 251 174 486 4072 Fragments arranged by size: 4072 889 863 748 486 385 304 251 213 174 115 95 70 54 BbeI G_GCGC'C Cuts at: 5679 5679 Size: 8719 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2911 928 Size: 971 1012 6736 Fragments arranged by size: 6736 1012 971 BbvCI CC'TCA_GC Cuts at: 2148 2166 2409 3704 4421 4845 5432 2148 Size: 18 243 1295 717 424 587 5435 Fragments arranged by size: 5435 1295 717 587 424 243 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6910 6944 1903 Size: 34 4973 34 3678 Fragments arranged by size: 4973 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3126 6611 7821 3126 Size: 3485 1210 4024 Fragments arranged by size: 4024 3485 1210 BclI T'GATC_A Cuts at: 1548 3498 3550 1548 Size: 1950 52 6717 Fragments arranged by size: 6717 1950 52 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3976 4229 4361 Size: 107 122 71 631 605 2304 253 132 Cuts at: 4361 4947 5569 7330 136 Size: 586 622 1761 1525 Fragments arranged by size: 2304 1761 1525 631 622 605 586 253 132 122 107 71 BglII A'GATC_T Cuts at: 8714 8714 Size: 8719 BlpI GC'TnA_GC Cuts at: 3980 5427 6136 3980 Size: 1447 709 6563 Fragments arranged by size: 6563 1447 709 Bme1580I G_kGCm'C Cuts at: 1790 2280 2748 3014 3028 3745 3776 3990 4104 Size: 490 468 266 14 717 31 214 114 Cuts at: 4104 4601 4962 5127 5169 5552 6780 7936 1790 Size: 497 361 165 42 383 1228 1156 2573 Fragments arranged by size: 2573 1228 1156 717 497 490 468 383 361 266 214 165 114 42 31 14 BmgBI CAC'GTC Cuts at: 1318 5281 1318 Size: 3963 4756 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2006 2154 2346 Size: 416 53 83 399 24 589 148 192 Cuts at: 2346 2543 4606 5788 6450 7408 442 Size: 197 2063 1182 662 958 1753 Fragments arranged by size: 2063 1753 1182 958 662 589 416 399 197 192 148 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 2576 3161 3218 3624 3804 3905 4019 Size: 1049 288 585 57 406 180 101 114 Cuts at: 4019 4626 4784 4886 4952 5024 5957 7379 1239 Size: 607 158 102 66 72 933 1422 2579 Fragments arranged by size: 2579 1422 1049 933 607 585 406 288 180 158 114 102 101 72 66 57 Bpu10I CC'TnA_GC Cuts at: 2148 2166 2409 3704 4421 4720 4845 5432 5898 Size: 18 243 1295 717 299 125 587 466 Cuts at: 5898 2148 Size: 4969 Fragments arranged by size: 4969 1295 717 587 466 299 243 125 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4514 6844 7709 8007 4514 Size: 2330 865 298 5226 Fragments arranged by size: 5226 2330 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 3459 3780 4442 5223 7382 882 Size: 617 858 1102 321 662 781 2159 2219 Fragments arranged by size: 2219 2159 1102 858 781 662 617 321 BsaAI yAC'GTr Cuts at: 493 1204 2725 3031 5716 6510 493 Size: 711 1521 306 2685 794 2702 Fragments arranged by size: 2702 2685 1521 794 711 306 BsaBI GATnn'nnATC Cuts at: 8713 8713 Size: 8719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2109 5676 6526 6908 Size: 53 83 186 936 576 3567 850 382 Cuts at: 6908 7543 275 Size: 635 1451 Fragments arranged by size: 3567 1451 936 850 635 576 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2445 3789 5442 5731 7152 7550 7824 7971 8382 Size: 1344 1653 289 1421 398 274 147 411 Cuts at: 8382 2445 Size: 2782 Fragments arranged by size: 2782 1653 1421 1344 411 398 289 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3354 3384 4062 4092 4734 Size: 30 165 30 1951 30 678 30 642 Cuts at: 4734 4764 1178 Size: 30 5133 Fragments arranged by size: 5133 1951 678 642 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 3477 4104 4166 4703 4862 5021 5054 Size: 997 1294 627 62 537 159 159 33 Cuts at: 5054 5108 5228 1186 Size: 54 120 4677 Fragments arranged by size: 4677 1294 997 627 537 159 159 120 62 54 33 BseYI C'CCAG_C Cuts at: 2341 3598 3901 4083 4623 4936 5126 5186 5261 Size: 1257 303 182 540 313 190 60 75 Cuts at: 5261 5420 5508 5559 5619 7922 8418 2341 Size: 159 88 51 60 2303 496 2642 Fragments arranged by size: 2642 2303 1257 540 496 313 303 190 182 159 88 75 60 60 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2423 3431 3437 3476 4892 5759 1163 Size: 573 687 1008 6 39 1416 867 4123 Fragments arranged by size: 4123 1416 1008 867 687 573 39 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2446 3814 6169 6930 7958 1540 Size: 275 186 445 1368 2355 761 1028 2301 Fragments arranged by size: 2355 2301 1368 1028 761 445 275 186 BsiHKAI G_wGCw'C Cuts at: 2651 2691 2748 2774 3014 3891 3990 4158 6780 Size: 40 57 26 240 877 99 168 2622 Cuts at: 6780 6865 7936 2651 Size: 85 1071 3434 Fragments arranged by size: 3434 2622 1071 877 240 168 99 85 57 40 26 BsmI GAATG_Cn' Cuts at: 3658 6230 6323 3658 Size: 2572 93 6054 Fragments arranged by size: 6054 2572 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5036 5036 Size: 8719 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2280 2651 2691 2748 2774 3014 3028 Size: 399 490 371 40 57 26 240 14 Cuts at: 3028 3745 3776 3891 3990 4104 4158 4462 4577 Size: 717 31 115 99 114 54 304 115 Cuts at: 4577 4601 4962 5032 5127 5169 5378 5552 6038 Size: 24 361 70 95 42 209 174 486 Cuts at: 6038 6780 6865 7936 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 717 490 486 399 371 361 304 240 209 174 115 115 114 99 95 85 70 57 54 42 40 31 26 24 14 BspEI T'CCGG_A Cuts at: 5442 5731 8382 5442 Size: 289 2651 5779 Fragments arranged by size: 5779 2651 289 BspHI T'CATG_A Cuts at: 2766 3547 6608 8338 2766 Size: 781 3061 1730 3147 Fragments arranged by size: 3147 3061 1730 781 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2268 3403 6074 844 Size: 1424 1135 2671 3489 Fragments arranged by size: 3489 2671 1424 1135 BsrBI CCG'CTC Cuts at: 1394 1823 3222 4062 5116 5902 6606 1394 Size: 429 1399 840 1054 786 704 3507 Fragments arranged by size: 3507 1399 1054 840 786 704 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2421 3574 3580 4924 7214 7388 65 Size: 1183 1173 1153 6 1344 2290 174 1396 Fragments arranged by size: 2290 1396 1344 1183 1173 1153 174 6 BsrFI r'CCGG_y Cuts at: 1700 1810 2379 2544 3047 4587 5061 7363 7550 Size: 110 569 165 503 1540 474 2302 187 Cuts at: 7550 1700 Size: 2869 Fragments arranged by size: 2869 2302 1540 569 503 474 187 165 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8719 BssSI C'ACGA_G Cuts at: 4859 6779 7791 4859 Size: 1920 1012 5787 Fragments arranged by size: 5787 1920 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8719 BstBI TT'CG_AA Cuts at: 7523 7523 Size: 8719 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8719 BstXI CCAn_nnnn'nTGG Cuts at: 2341 2428 2576 2729 3119 3439 4054 4177 5261 Size: 87 148 153 390 320 615 123 1084 Cuts at: 5261 5764 5778 2341 Size: 503 14 5282 Fragments arranged by size: 5282 1084 615 503 390 320 153 148 123 87 14 BstYI r'GATC_y Cuts at: 1972 3100 6049 6416 6801 6818 8270 8356 8368 Size: 1128 2949 367 385 17 1452 86 12 Cuts at: 8368 8714 1972 Size: 346 1977 Fragments arranged by size: 2949 1977 1452 1128 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2862 5108 2862 Size: 2246 6473 BtgI C'CryG_G Cuts at: 513 1065 3019 3300 4023 4065 4233 4968 5329 Size: 552 1954 281 723 42 168 735 361 Cuts at: 5329 513 Size: 3903 Fragments arranged by size: 3903 1954 735 723 552 361 281 168 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7980 Fragments arranged by size: 7980 683 56 BtsI GCAGTG_nn' Cuts at: 2830 3042 4097 4380 4404 5771 5927 6235 7029 Size: 212 1055 283 24 1367 156 308 794 Cuts at: 7029 7049 2830 Size: 20 4500 Fragments arranged by size: 4500 1367 1055 794 308 283 212 156 24 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3858 3893 529 Size: 35 3294 35 5355 Fragments arranged by size: 5355 3294 35 35 DraI TTT'AAA Cuts at: 6023 6023 Size: 8719 DraIII CAC_nnn'GTG Cuts at: 3034 3034 Size: 8719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4214 7726 809 Size: 507 2898 3512 1802 Fragments arranged by size: 3512 2898 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2041 2876 3247 Size: 54 1425 50 275 18 211 835 371 Cuts at: 3247 3838 4026 4230 4236 5241 5450 5570 5869 Size: 591 188 204 6 1005 209 120 299 Cuts at: 5869 6166 7055 8684 8 Size: 297 889 1629 43 Fragments arranged by size: 1629 1425 1005 889 835 591 371 299 297 275 211 209 204 188 120 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 6166 1537 Size: 275 4354 4090 Fragments arranged by size: 4354 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3702 6649 1353 Size: 2349 2947 3423 Fragments arranged by size: 3423 2947 2349 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4364 7306 7680 7826 387 Size: 1064 138 2775 2942 374 146 1280 Fragments arranged by size: 2942 2775 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3889 4156 3889 Size: 267 8452 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2576 3161 3218 3624 3804 3905 Size: 393 656 288 585 57 406 180 101 Cuts at: 3905 4019 4560 4626 4663 4784 4886 4919 4952 Size: 114 541 66 37 121 102 33 33 Cuts at: 4952 5024 5338 5446 5957 6782 7379 8166 8434 Size: 72 314 108 511 825 597 787 268 Cuts at: 8434 1239 Size: 1524 Fragments arranged by size: 1524 825 787 656 597 585 541 511 406 393 314 288 268 180 121 114 108 102 101 72 66 57 37 33 33 EcoNI CCTnn'n_nnAGG Cuts at: 4889 5036 4889 Size: 147 8572 EcoO109I rG'GnC_Cy Cuts at: 2152 2345 2599 2866 3024 3328 3365 3517 3640 Size: 193 254 267 158 304 37 152 123 Cuts at: 3640 3708 4100 4101 4959 5104 5123 5293 5549 Size: 68 392 1 858 145 19 170 256 Cuts at: 5549 5639 5832 2152 Size: 90 193 5039 Fragments arranged by size: 5039 858 392 304 267 256 254 193 193 170 158 152 145 123 90 68 37 19 1 EcoRI G'AATT_C Cuts at: 6028 6406 6028 Size: 378 8341 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3943 3943 Size: 8719 FspI TGC'GCA Cuts at: 5700 7225 5700 Size: 1525 7194 FspAI rTGC'GCAy Cuts at: 5700 5700 Size: 8719 HaeII r_GCGC'y Cuts at: 1678 2377 2623 3561 4039 5440 5608 5679 7866 Size: 699 246 938 478 1401 168 71 2187 Cuts at: 7866 1678 Size: 2531 Fragments arranged by size: 2531 2187 1401 938 699 478 246 168 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2901 2933 3354 3386 4537 Size: 32 135 32 1329 32 421 32 1151 Cuts at: 4537 4569 4734 4766 4814 4846 5187 5219 5285 Size: 32 165 32 48 32 341 32 66 Cuts at: 5285 5317 5395 5427 7360 7392 7434 7466 1373 Size: 32 78 32 1933 32 42 32 2626 Fragments arranged by size: 2626 1933 1329 1151 421 341 165 135 78 66 48 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5489 6063 6309 669 Size: 988 281 3551 574 246 3079 Fragments arranged by size: 3551 3079 988 574 281 246 HindIII A'AGCT_T Cuts at: 748 3106 748 Size: 2358 6361 HpaI GTT'AAC Cuts at: 6309 6309 Size: 8719 KasI G'GCGC_C Cuts at: 5675 5675 Size: 8719 KpnI G_GTAC'C Cuts at: 6044 6044 Size: 8719 MfeI C'AATT_G Cuts at: 5310 6318 5310 Size: 1008 7711 MluI A'CGCG_T Cuts at: 8595 8595 Size: 8719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3340 3367 7833 8017 1305 Size: 2035 27 4466 184 2007 Fragments arranged by size: 4466 2035 2007 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 2043 3840 4028 10 Size: 54 1425 343 211 1797 188 4701 Fragments arranged by size: 4701 1797 1425 343 211 188 54 MslI CAynn'nnrTG Cuts at: 518 1166 2025 2727 3738 6677 7036 7195 518 Size: 648 859 702 1011 2939 359 159 2042 Fragments arranged by size: 2939 2042 1011 859 702 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2147 2408 2638 3021 3253 3602 5056 5396 Size: 541 261 230 383 232 349 1454 340 Cuts at: 5396 6135 6812 7960 8205 1606 Size: 739 677 1148 245 2120 Fragments arranged by size: 2120 1454 1148 739 677 541 383 349 340 261 245 232 230 NaeI GCC'GGC Cuts at: 2546 3049 2546 Size: 503 8216 NarI GG'CG_CC Cuts at: 5676 5676 Size: 8719 NcoI C'CATG_G Cuts at: 513 1065 3300 4968 513 Size: 552 2235 1668 4264 Fragments arranged by size: 4264 2235 1668 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8719 NgoMIV G'CCGG_C Cuts at: 2544 3047 2544 Size: 503 8216 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8719 NotI GC'GGCC_GC Cuts at: 6166 6166 Size: 8719 NspI r_CATG'y Cuts at: 1132 1229 2503 2915 4758 6077 7622 1132 Size: 97 1274 412 1843 1319 1545 2229 Fragments arranged by size: 2229 1843 1545 1319 1274 412 97 PasI CC'CwG_GG Cuts at: 1348 3093 3974 4328 4387 4577 4815 5415 5837 Size: 1745 881 354 59 190 238 600 422 Cuts at: 5837 1348 Size: 4230 Fragments arranged by size: 4230 1745 881 600 422 354 238 190 59 PciI A'CATG_T Cuts at: 2911 7618 2911 Size: 4707 4012 PflMI CCAn_nnn'nTGG Cuts at: 1108 3034 3997 4930 4969 5388 1108 Size: 1926 963 933 39 419 4439 Fragments arranged by size: 4439 1926 963 933 419 39 PfoI T'CCnGG_A Cuts at: 2625 4281 2625 Size: 1656 7063 PmeI GTTT'AAAC Cuts at: 6023 6023 Size: 8719 PmlI CAC'GTG Cuts at: 2725 3031 2725 Size: 306 8413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6788 6820 8301 8333 6788 Size: 32 1481 32 7174 Fragments arranged by size: 7174 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2599 2866 3365 3640 3708 5293 5832 2599 Size: 267 499 275 68 1585 539 5486 Fragments arranged by size: 5486 1585 539 499 275 267 68 PshAI GACnn'nnGTC Cuts at: 1613 3734 1613 Size: 2121 6598 PsiI TTA'TAA Cuts at: 6289 6289 Size: 8719 PspOMI G'GGCC_C Cuts at: 2276 3024 4100 4958 5123 5548 2276 Size: 748 1076 858 165 425 5447 Fragments arranged by size: 5447 1076 858 748 425 165 PstI C_TGCA'G Cuts at: 830 2106 3542 3936 3957 5542 6071 830 Size: 1276 1436 394 21 1585 529 3478 Fragments arranged by size: 3478 1585 1436 1276 529 394 21 PvuI CG_AT'CG Cuts at: 2001 3814 2001 Size: 1813 6906 PvuII CAG'CTG Cuts at: 2147 2408 3602 5056 5396 2147 Size: 261 1194 1454 340 5470 Fragments arranged by size: 5470 1454 1194 340 261 SacI G_AGCT'C Cuts at: 3891 4158 3891 Size: 267 8452 SacII CC_GC'GG Cuts at: 3022 3022 Size: 8719 SalI G'TCGA_C Cuts at: 1936 6061 1936 Size: 4125 4594 SanDI GG'GwC_CC Cuts at: 3640 5832 3640 Size: 2192 6527 SapI GCTCTTCn'nnn_ Cuts at: 3702 3702 Size: 8719 SbfI CC_TGCA'GG Cuts at: 6071 6071 Size: 8719 ScaI AGT'ACT Cuts at: 6967 8679 6967 Size: 1712 7007 SexAI A'CCwGG_T Cuts at: 4254 4254 Size: 8719 SfcI C'TryA_G Cuts at: 826 1045 2102 2633 3373 3538 3932 3953 4793 Size: 219 1057 531 740 165 394 21 840 Cuts at: 4793 5066 5538 6067 7202 7883 8074 826 Size: 273 472 529 1135 681 191 1471 Fragments arranged by size: 1471 1135 1057 840 740 681 531 529 472 394 273 219 191 165 21 SfoI GGC'GCC Cuts at: 5677 5677 Size: 8719 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8719 SmaI CCC'GGG Cuts at: 1872 6046 1872 Size: 4174 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4529 6823 7724 7986 820 Size: 197 926 2586 2294 901 262 1553 Fragments arranged by size: 2586 2294 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5716 6510 493 Size: 5223 794 2702 Fragments arranged by size: 5223 2702 794 SpeI A'CTAG_T Cuts at: 152 152 Size: 8719 SphI G_CATG'C Cuts at: 1132 2503 6077 1132 Size: 1371 3574 3774 Fragments arranged by size: 3774 3574 1371 SspI AAT'ATT Cuts at: 5 52 6643 5 Size: 47 6591 2081 Fragments arranged by size: 6591 2081 47 StuI AGG'CCT Cuts at: 1517 2660 4074 4746 4857 5577 5914 1517 Size: 1143 1414 672 111 720 337 4322 Fragments arranged by size: 4322 1414 1143 720 672 337 111 StyI C'CwwG_G Cuts at: 513 1065 3225 3300 3522 4176 4968 5915 6158 Size: 552 2160 75 222 654 792 947 243 Cuts at: 6158 513 Size: 3074 Fragments arranged by size: 3074 2160 947 792 654 552 243 222 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2525 2789 3886 4379 5500 6746 6763 Size: 1255 945 264 1097 493 1121 1246 17 Cuts at: 6763 6916 7101 7546 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1246 1121 1097 945 493 445 264 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6965 8677 96 Size: 274 80 33 51 1443 4988 1712 138 Fragments arranged by size: 4988 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4062 4094 4277 4309 8679 8711 4062 Size: 32 183 32 4370 32 4070 Fragments arranged by size: 4370 4070 183 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8719 XbaI T'CTAG_A Cuts at: 6055 6055 Size: 8719 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3035 5597 5632 3035 Size: 2562 35 6122 Fragments arranged by size: 6122 2562 35 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8719 XmaI C'CCGG_G Cuts at: 1870 6044 1870 Size: 4174 4545 XmnI GAAnn'nnTTC Cuts at: 3923 6848 3923 Size: 2925 5794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6527 276 Size: 53 83 186 936 4993 2468 Fragments arranged by size: 4993 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FseI NruI NsiI PacI PspXI PsrI RsrII SfiI SrfI SwaI