Restriction map for
pFN21AA0361
(Linear) (Six-base) MAPSORT of: KIAA0361.seq Check: 1201 from: 1 to: 4014
KIAA0361.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1398 4014
Size: 1398 2616
AccI GT'mk_AC
Cuts at: 0 1367 4014
Size: 1367 2647
AclI AA'CG_TT
Cuts at: 0 1746 4014
Size: 1746 2268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2555 2658 2914 3333 3441 4014
Size: 2555 103 256 419 108 573
Fragments arranged by size:
2555 573 419 256 108 103
AfeI AGC'GCT
Cuts at: 0 616 2032 4014
Size: 616 1416 1982
Fragments arranged by size:
1982 1416 616
AflIII A'CryG_T
Cuts at: 0 906 4014
Size: 906 3108
AhdI GACnn_n'nnGTC
Cuts at: 0 910 4014
Size: 910 3104
AlwNI CAG_nnn'CTG
Cuts at: 0 55 735 1956 1992 2509 2691 2892 3394
Size: 55 680 1221 36 517 182 201 502
Cuts at: 3394 4014
Size: 620
Fragments arranged by size:
1221 680 620 517 502 201 182 55
36
ApaI G_GGCC'C
Cuts at: 0 275 1023 2099 2957 3122 3547 4014
Size: 275 748 1076 858 165 425 467
Fragments arranged by size:
1076 858 748 467 425 275 165
ApaLI G'TGCA_C
Cuts at: 0 739 1005 1981 4014
Size: 739 266 976 2033
Fragments arranged by size:
2033 976 739 266
ApoI r'AATT_y
Cuts at: 0 802 1119 4014
Size: 802 317 2895
Fragments arranged by size:
2895 802 317
AvaI C'yCGr_G
Cuts at: 0 221 639 2429 2587 3188 4014
Size: 221 418 1790 158 601 826
Fragments arranged by size:
1790 826 601 418 221 158
BanI G'GyrC_C
Cuts at: 0 1768 3670 4014
Size: 1768 1902 344
Fragments arranged by size:
1902 1768 344
BanII G_rGCy'C
Cuts at: 0 275 1023 1886 2099 2153 2457 2572 2957
Size: 275 748 863 213 54 304 115 385
Cuts at: 2957 3027 3122 3373 3547 4014
Size: 70 95 251 174 467
Fragments arranged by size:
863 748 467 385 304 275 251 213
174 115 95 70 54
BbeI G_GCGC'C
Cuts at: 0 3674 4014
Size: 3674 340
BbsI GAAGACnn'nnnn_
Cuts at: 0 906 4014
Size: 906 3108
BbvCI CC'TCA_GC
Cuts at: 0 143 161 404 1699 2416 2840 3427 4014
Size: 143 18 243 1295 717 424 587 587
Fragments arranged by size:
1295 717 587 587 424 243 143 18
BciVI GTATCCnnnnn_n'
Cuts at: 0 1121 4014
Size: 1121 2893
BclI T'GATC_A
Cuts at: 0 1493 1545 4014
Size: 1493 52 2469
Fragments arranged by size:
2469 1493 52
BglI GCCn_nnn'nGGC
Cuts at: 0 1971 2224 2356 2942 3564 4014
Size: 1971 253 132 586 622 450
Fragments arranged by size:
1971 622 586 450 253 132
BlpI GC'TnA_GC
Cuts at: 0 1975 3422 4014
Size: 1975 1447 592
Fragments arranged by size:
1975 1447 592
Bme1580I G_kGCm'C
Cuts at: 0 275 743 1009 1023 1740 1771 1985 2099
Size: 275 468 266 14 717 31 214 114
Cuts at: 2099 2596 2957 3122 3164 3547 4014
Size: 497 361 165 42 383 467
Fragments arranged by size:
717 497 468 467 383 361 275 266
214 165 114 42 31 14
BmgBI CAC'GTC
Cuts at: 0 3276 4014
Size: 3276 738
BmrI ACTGGGnnnn_n'
Cuts at: 0 1 149 341 538 2601 3783 4014
Size: 1 148 192 197 2063 1182 231
Fragments arranged by size:
2063 1182 231 197 192 148 1
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 571 1156 1213 1619 1799 1900 2014
Size: 283 288 585 57 406 180 101 114
Cuts at: 2014 2621 2779 2881 2947 3019 3952 4014
Size: 607 158 102 66 72 933 62
Fragments arranged by size:
933 607 585 406 288 283 180 158
114 102 101 72 66 62 57
Bpu10I CC'TnA_GC
Cuts at: 0 143 161 404 1699 2416 2715 2840 3427
Size: 143 18 243 1295 717 299 125 587
Cuts at: 3427 3893 4014
Size: 466 121
Fragments arranged by size:
1295 717 587 466 299 243 143 125
121 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2509 4014
Size: 2509 1505
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 1454 1775 2437 3218 4014
Size: 352 1102 321 662 781 796
Fragments arranged by size:
1102 796 781 662 352 321
BsaAI yAC'GTr
Cuts at: 0 720 1026 3711 4014
Size: 720 306 2685 303
Fragments arranged by size:
2685 720 306 303
BsaHI Gr'CG_yC
Cuts at: 0 104 3671 4014
Size: 104 3567 343
Fragments arranged by size:
3567 343 104
BsaWI w'CCGG_w
Cuts at: 0 440 1784 3437 3726 4014
Size: 440 1344 1653 289 288
Fragments arranged by size:
1653 1344 440 289 288
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1349 1379 2057 2087 2729 2759 4014
Size: 1349 30 678 30 642 30 1255
Fragments arranged by size:
1349 1255 678 642 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 1472 2099 2161 2698 2857 3016 3049
Size: 178 1294 627 62 537 159 159 33
Cuts at: 3049 3103 3223 4014
Size: 54 120 791
Fragments arranged by size:
1294 791 627 537 178 159 159 120
62 54 33
BseYI C'CCAG_C
Cuts at: 0 336 1593 1896 2078 2618 2931 3121 3181
Size: 336 1257 303 182 540 313 190 60
Cuts at: 3181 3256 3415 3503 3554 3614 4014
Size: 75 159 88 51 60 400
Fragments arranged by size:
1257 540 400 336 313 303 190 182
159 88 75 60 60 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1426 1432 1471 2887 3754 4014
Size: 418 1008 6 39 1416 867 260
Fragments arranged by size:
1416 1008 867 418 260 39 6
BsiEI CG_ry'CG
Cuts at: 0 441 1809 4014
Size: 441 1368 2205
Fragments arranged by size:
2205 1368 441
BsiHKAI G_wGCw'C
Cuts at: 0 646 686 743 769 1009 1886 1985 2153
Size: 646 40 57 26 240 877 99 168
Cuts at: 2153 4014
Size: 1861
Fragments arranged by size:
1861 877 646 240 168 99 57 40
26
BsmI GAATG_Cn'
Cuts at: 0 1653 4014
Size: 1653 2361
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3031 4014
Size: 3031 983
Bsp1286I G_dGCh'C
Cuts at: 0 275 646 686 743 769 1009 1023 1740
Size: 275 371 40 57 26 240 14 717
Cuts at: 1740 1771 1886 1985 2099 2153 2457 2572 2596
Size: 31 115 99 114 54 304 115 24
Cuts at: 2596 2957 3027 3122 3164 3373 3547 4014
Size: 361 70 95 42 209 174 467
Fragments arranged by size:
717 467 371 361 304 275 240 209
174 115 115 114 99 95 70 57
54 42 40 31 26 24 14
BspEI T'CCGG_A
Cuts at: 0 3437 3726 4014
Size: 3437 289 288
Fragments arranged by size:
3437 289 288
BspHI T'CATG_A
Cuts at: 0 761 1542 4014
Size: 761 781 2472
Fragments arranged by size:
2472 781 761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 263 1398 4014
Size: 263 1135 2616
Fragments arranged by size:
2616 1135 263
BsrBI CCG'CTC
Cuts at: 0 1217 2057 3111 3897 4014
Size: 1217 840 1054 786 117
Fragments arranged by size:
1217 1054 840 786 117
BsrDI GCAATG_nn'
Cuts at: 0 416 1569 1575 2919 4014
Size: 416 1153 6 1344 1095
Fragments arranged by size:
1344 1153 1095 416 6
BsrFI r'CCGG_y
Cuts at: 0 374 539 1042 2582 3056 4014
Size: 374 165 503 1540 474 958
Fragments arranged by size:
1540 958 503 474 374 165
BssSI C'ACGA_G
Cuts at: 0 2854 4014
Size: 2854 1160
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 423 571 724 1114 1434 2049 2172
Size: 336 87 148 153 390 320 615 123
Cuts at: 2172 3256 3759 3773 4014
Size: 1084 503 14 241
Fragments arranged by size:
1084 615 503 390 336 320 241 153
148 123 87 14
BstYI r'GATC_y
Cuts at: 0 1095 4014
Size: 1095 2919
Bsu36I CC'TnA_GG
Cuts at: 0 857 3103 4014
Size: 857 2246 911
Fragments arranged by size:
2246 911 857
BtgI C'CryG_G
Cuts at: 0 1014 1295 2018 2060 2228 2963 3324 4014
Size: 1014 281 723 42 168 735 361 690
Fragments arranged by size:
1014 735 723 690 361 281 168 42
BtsI GCAGTG_nn'
Cuts at: 0 825 1037 2092 2375 2399 3766 3922 4014
Size: 825 212 1055 283 24 1367 156 92
Fragments arranged by size:
1367 1055 825 283 212 156 92 24
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1853 1888 4014
Size: 1853 35 2126
Fragments arranged by size:
2126 1853 35
DraIII CAC_nnn'GTG
Cuts at: 0 1029 4014
Size: 1029 2985
DrdI GACnn_nn'nnGTC
Cuts at: 0 2209 4014
Size: 2209 1805
EaeI y'GGCC_r
Cuts at: 0 36 871 1242 1833 2021 2225 2231 3236
Size: 36 835 371 591 188 204 6 1005
Cuts at: 3236 3445 3565 3864 4014
Size: 209 120 299 150
Fragments arranged by size:
1005 835 591 371 299 209 204 188
150 120 36 6
EarI CTCTTCn'nnn_
Cuts at: 0 1697 4014
Size: 1697 2317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2359 4014
Size: 2359 1655
EcoICRI GAG'CTC
Cuts at: 0 1884 2151 4014
Size: 1884 267 1863
Fragments arranged by size:
1884 1863 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 571 1156 1213 1619 1799 1900 2014
Size: 283 288 585 57 406 180 101 114
Cuts at: 2014 2555 2621 2658 2779 2881 2914 2947 3019
Size: 541 66 37 121 102 33 33 72
Cuts at: 3019 3333 3441 3952 4014
Size: 314 108 511 62
Fragments arranged by size:
585 541 511 406 314 288 283 180
121 114 108 102 101 72 66 62
57 37 33 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2884 3031 4014
Size: 2884 147 983
Fragments arranged by size:
2884 983 147
EcoO109I rG'GnC_Cy
Cuts at: 0 147 340 594 861 1019 1323 1360 1512
Size: 147 193 254 267 158 304 37 152
Cuts at: 1512 1635 1703 2095 2096 2954 3099 3118 3288
Size: 123 68 392 1 858 145 19 170
Cuts at: 3288 3544 3634 3827 4014
Size: 256 90 193 187
Fragments arranged by size:
858 392 304 267 256 254 193 193
187 170 158 152 147 145 123 90
68 37 19 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1938 4014
Size: 1938 2076
FspI TGC'GCA
Cuts at: 0 3695 4014
Size: 3695 319
FspAI rTGC'GCAy
Cuts at: 0 3695 4014
Size: 3695 319
HaeII r_GCGC'y
Cuts at: 0 372 618 1556 2034 3435 3603 3674 4014
Size: 372 246 938 478 1401 168 71 340
Fragments arranged by size:
1401 938 478 372 340 246 168 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 896 928 1349 1381 2532 2564 2729 2761
Size: 896 32 421 32 1151 32 165 32
Cuts at: 2761 2809 2841 3182 3214 3280 3312 3390 3422
Size: 48 32 341 32 66 32 78 32
Cuts at: 3422 4014
Size: 592
Fragments arranged by size:
1151 896 592 421 341 165 78 66
48 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 3484 4014
Size: 3484 530
HindIII A'AGCT_T
Cuts at: 0 1101 4014
Size: 1101 2913
KasI G'GCGC_C
Cuts at: 0 3670 4014
Size: 3670 344
MfeI C'AATT_G
Cuts at: 0 3305 4014
Size: 3305 709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1335 1362 4014
Size: 1335 27 2652
Fragments arranged by size:
2652 1335 27
MscI TGG'CCA
Cuts at: 0 38 1835 2023 4014
Size: 38 1797 188 1991
Fragments arranged by size:
1991 1797 188 38
MslI CAynn'nnrTG
Cuts at: 0 20 722 1733 4014
Size: 20 702 1011 2281
Fragments arranged by size:
2281 1011 702 20
MspA1I CmG'CkG
Cuts at: 0 142 403 633 1016 1248 1597 3051 3391
Size: 142 261 230 383 232 349 1454 340
Cuts at: 3391 4014
Size: 623
Fragments arranged by size:
1454 623 383 349 340 261 232 230
142
NaeI GCC'GGC
Cuts at: 0 541 1044 4014
Size: 541 503 2970
Fragments arranged by size:
2970 541 503
NarI GG'CG_CC
Cuts at: 0 3671 4014
Size: 3671 343
NcoI C'CATG_G
Cuts at: 0 1295 2963 4014
Size: 1295 1668 1051
Fragments arranged by size:
1668 1295 1051
NgoMIV G'CCGG_C
Cuts at: 0 539 1042 4014
Size: 539 503 2972
Fragments arranged by size:
2972 539 503
NspI r_CATG'y
Cuts at: 0 498 910 2753 4014
Size: 498 412 1843 1261
Fragments arranged by size:
1843 1261 498 412
PasI CC'CwG_GG
Cuts at: 0 1088 1969 2323 2382 2572 2810 3410 3832
Size: 1088 881 354 59 190 238 600 422
Cuts at: 3832 4014
Size: 182
Fragments arranged by size:
1088 881 600 422 354 238 190 182
59
PciI A'CATG_T
Cuts at: 0 906 4014
Size: 906 3108
PflMI CCAn_nnn'nTGG
Cuts at: 0 1029 1992 2925 2964 3383 4014
Size: 1029 963 933 39 419 631
Fragments arranged by size:
1029 963 933 631 419 39
PfoI T'CCnGG_A
Cuts at: 0 620 2276 4014
Size: 620 1656 1738
Fragments arranged by size:
1738 1656 620
PmlI CAC'GTG
Cuts at: 0 720 1026 4014
Size: 720 306 2988
Fragments arranged by size:
2988 720 306
PpuMI rG'GwC_Cy
Cuts at: 0 594 861 1360 1635 1703 3288 3827 4014
Size: 594 267 499 275 68 1585 539 187
Fragments arranged by size:
1585 594 539 499 275 267 187 68
PshAI GACnn'nnGTC
Cuts at: 0 1729 4014
Size: 1729 2285
PspOMI G'GGCC_C
Cuts at: 0 271 1019 2095 2953 3118 3543 4014
Size: 271 748 1076 858 165 425 471
Fragments arranged by size:
1076 858 748 471 425 271 165
PstI C_TGCA'G
Cuts at: 0 101 1537 1931 1952 3537 4014
Size: 101 1436 394 21 1585 477
Fragments arranged by size:
1585 1436 477 394 101 21
PvuI CG_AT'CG
Cuts at: 0 1809 4014
Size: 1809 2205
PvuII CAG'CTG
Cuts at: 0 142 403 1597 3051 3391 4014
Size: 142 261 1194 1454 340 623
Fragments arranged by size:
1454 1194 623 340 261 142
SacI G_AGCT'C
Cuts at: 0 1886 2153 4014
Size: 1886 267 1861
Fragments arranged by size:
1886 1861 267
SacII CC_GC'GG
Cuts at: 0 1017 4014
Size: 1017 2997
SanDI GG'GwC_CC
Cuts at: 0 1635 3827 4014
Size: 1635 2192 187
Fragments arranged by size:
2192 1635 187
SapI GCTCTTCn'nnn_
Cuts at: 0 1697 4014
Size: 1697 2317
SexAI A'CCwGG_T
Cuts at: 0 2249 4014
Size: 2249 1765
SfcI C'TryA_G
Cuts at: 0 97 628 1368 1533 1927 1948 2788 3061
Size: 97 531 740 165 394 21 840 273
Cuts at: 3061 3533 4014
Size: 472 481
Fragments arranged by size:
840 740 531 481 472 394 273 165
97 21
SfoI GGC'GCC
Cuts at: 0 3672 4014
Size: 3672 342
SmlI C'TyrA_G
Cuts at: 0 2524 4014
Size: 2524 1490
SnaBI TAC'GTA
Cuts at: 0 3711 4014
Size: 3711 303
SphI G_CATG'C
Cuts at: 0 498 4014
Size: 498 3516
StuI AGG'CCT
Cuts at: 0 655 2069 2741 2852 3572 3909 4014
Size: 655 1414 672 111 720 337 105
Fragments arranged by size:
1414 720 672 655 337 111 105
StyI C'CwwG_G
Cuts at: 0 1220 1295 1517 2171 2963 3910 4014
Size: 1220 75 222 654 792 947 104
Fragments arranged by size:
1220 947 792 654 222 104 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 520 784 1881 2374 3495 4014
Size: 520 264 1097 493 1121 519
Fragments arranged by size:
1121 1097 520 519 493 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2057 2089 2272 2304 4014
Size: 2057 32 183 32 1710
Fragments arranged by size:
2057 1710 183 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1030 3592 3627 4014
Size: 1030 2562 35 387
Fragments arranged by size:
2562 1030 387 35
XmnI GAAnn'nnTTC
Cuts at: 0 1918 4014
Size: 1918 2096
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AhdI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstXI
BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI
PstI PvuI PvuII SacI SacII SanDI SapI SexAI
SfcI SfoI SmlI SnaBI SphI StuI StyI TaqII
TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AflII AgeI AleI AlfI AloI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BfrBI BglII
BmtI BplI BsaBI BsiWI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I BtgZI ClaI DraI EagI EcoRI EcoRV
FseI HpaI KpnI MluI NdeI NheI NotI NruI
NsiI PacI PmeI PpiI PsiI PspXI PsrI RsrII
SalI SbfI ScaI SfiI SgfI SgrAI SmaI SpeI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI
ZraI