(Circular) (Six-base) MAPSORT of: pFN21AA0338.seq Check: 5312 from: 1 to: 7348 pFN21AA0338 7348 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5158 278 Size: 53 83 186 936 3622 2468 Fragments arranged by size: 3622 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2859 4593 4691 1937 Size: 922 1734 98 4594 Fragments arranged by size: 4594 1734 922 98 Acc65I G'GTAC_C Cuts at: 4669 4669 Size: 7348 AclI AA'CG_TT Cuts at: 1563 5475 5848 1563 Size: 3912 373 3063 Fragments arranged by size: 3912 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3539 3578 3806 5411 6795 7063 1632 Size: 1907 39 228 1605 1384 268 1917 Fragments arranged by size: 1917 1907 1605 1384 268 228 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7151 AflIII A'CryG_T Cuts at: 1203 6247 7224 1203 Size: 5044 977 1327 Fragments arranged by size: 5044 1327 977 AgeI A'CCGG_T Cuts at: 3075 6179 3075 Size: 3104 4244 AhdI GACnn_n'nnGTC Cuts at: 6077 6077 Size: 7348 AleI CACnn'nnGTG Cuts at: 1166 2096 1166 Size: 930 6418 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7348 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3764 3796 3764 Size: 32 7316 AlwNI CAG_nnn'CTG Cuts at: 2368 3353 3407 4528 5091 6663 2368 Size: 985 54 1121 563 1572 3053 Fragments arranged by size: 3053 1572 1121 985 563 54 ApaI G_GGCC'C Cuts at: 3730 4000 3730 Size: 270 7078 ApaLI G'TGCA_C Cuts at: 5405 6561 5405 Size: 1156 6192 ApoI r'AATT_y Cuts at: 1493 2506 4657 4883 5035 7144 1493 Size: 1013 2151 226 152 2109 1697 Fragments arranged by size: 2151 2109 1697 1013 226 152 AseI AT'TA_AT Cuts at: 160 1032 5902 160 Size: 872 4870 1606 Fragments arranged by size: 4870 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7348 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2745 2800 3723 3978 4673 1870 Size: 73 126 676 55 923 255 695 4545 Fragments arranged by size: 4545 923 695 676 255 126 73 55 BamHI G'GATC_C Cuts at: 4678 5045 4678 Size: 367 6981 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3650 4394 4669 6124 618 Size: 325 217 627 1863 744 275 1455 1842 Fragments arranged by size: 1863 1842 1455 744 627 325 275 217 BanII G_rGCy'C Cuts at: 1391 2654 2724 3147 3176 3546 3567 3695 3730 Size: 1263 70 423 29 370 21 128 35 Cuts at: 3730 3933 4000 4086 4667 1391 Size: 203 67 86 581 4072 Fragments arranged by size: 4072 1263 581 423 370 203 128 86 70 67 35 29 21 BbeI G_GCGC'C Cuts at: 4398 4398 Size: 7348 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2565 3912 928 Size: 971 666 1347 4364 Fragments arranged by size: 4364 1347 971 666 BbvCI CC'TCA_GC Cuts at: 2691 3408 3683 3858 2691 Size: 717 275 175 6181 Fragments arranged by size: 6181 717 275 175 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2060 2094 3883 3917 4035 4069 5539 Size: 34 123 34 1789 34 118 34 1470 Cuts at: 5539 5573 1903 Size: 34 3678 Fragments arranged by size: 3678 1789 1470 123 118 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5240 6450 5240 Size: 1210 6138 BclI T'GATC_A Cuts at: 1548 1548 Size: 7348 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5959 136 Size: 107 122 71 631 605 4287 1525 Fragments arranged by size: 4287 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2982 7343 2982 Size: 4361 2987 BlpI GC'TnA_GC Cuts at: 2670 4765 2670 Size: 2095 5253 Bme1580I G_kGCm'C Cuts at: 1790 3653 3730 4000 5409 6565 1790 Size: 1863 77 270 1409 1156 2573 Fragments arranged by size: 2573 1863 1409 1156 270 77 BmgBI CAC'GTC Cuts at: 1318 3719 1318 Size: 2401 4947 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2657 3607 4069 Size: 416 53 83 399 24 1240 950 462 Cuts at: 4069 4506 5079 6037 442 Size: 437 573 958 1753 Fragments arranged by size: 1753 1240 958 950 573 462 437 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7348 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2149 2149 Size: 7348 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2774 3805 3872 6008 1239 Size: 1535 1031 67 2136 2579 Fragments arranged by size: 2579 2136 1535 1031 67 Bpu10I CC'TnA_GC Cuts at: 2046 2691 3141 3408 3683 3858 3927 2046 Size: 645 450 267 275 175 69 5467 Fragments arranged by size: 5467 645 450 275 267 175 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2963 4268 5473 6338 6636 2963 Size: 1305 1205 865 298 3675 Fragments arranged by size: 3675 1305 1205 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4247 6011 882 Size: 617 2748 1764 2219 Fragments arranged by size: 2748 2219 1764 617 BsaAI yAC'GTr Cuts at: 493 1204 5139 493 Size: 711 3935 2702 Fragments arranged by size: 3935 2702 711 BsaBI GATnn'nnATC Cuts at: 4512 7342 4512 Size: 2830 4518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4395 5155 5537 6172 Size: 53 83 186 936 2862 760 382 635 Cuts at: 6172 275 Size: 1451 Fragments arranged by size: 2862 1451 936 760 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2490 3075 3183 5781 6179 6453 6600 7011 2490 Size: 585 108 2598 398 274 147 411 2827 Fragments arranged by size: 2827 2598 585 411 398 274 147 108 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2318 2348 1178 Size: 30 165 30 915 30 6178 Fragments arranged by size: 6178 915 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2210 2708 2771 3010 3425 3613 3713 Size: 880 144 498 63 239 415 188 100 Cuts at: 3713 4634 1186 Size: 921 3900 Fragments arranged by size: 3900 921 880 498 415 239 188 144 100 63 BseYI C'CCAG_C Cuts at: 2154 2243 2547 2674 3319 4001 4185 6551 7047 Size: 89 304 127 645 682 184 2366 496 Cuts at: 7047 2154 Size: 2455 Fragments arranged by size: 2455 2366 682 645 496 304 184 127 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2684 2849 3228 1163 Size: 573 948 165 379 5283 Fragments arranged by size: 5283 948 573 379 165 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3937 4798 5559 6587 1540 Size: 275 186 1936 861 761 1028 2301 Fragments arranged by size: 2301 1936 1028 861 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2724 3050 3546 3821 3933 5409 5494 6565 2724 Size: 326 496 275 112 1476 85 1071 3507 Fragments arranged by size: 3507 1476 1071 496 326 275 112 85 BsmI GAATG_Cn' Cuts at: 2836 4859 4952 2836 Size: 2023 93 5232 Fragments arranged by size: 5232 2023 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2654 2724 3050 3147 3176 3546 3567 Size: 399 864 70 326 97 29 370 21 Cuts at: 3567 3653 3695 3730 3821 3933 4000 4086 4667 Size: 86 42 35 91 112 67 86 581 Cuts at: 4667 5409 5494 6565 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 864 742 581 399 370 326 112 97 91 86 86 85 70 67 42 35 29 21 BspEI T'CCGG_A Cuts at: 2490 7011 2490 Size: 4521 2827 BspHI T'CATG_A Cuts at: 5237 6967 5237 Size: 1730 5618 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2871 4180 4703 844 Size: 2027 1309 523 3489 Fragments arranged by size: 3489 2027 1309 523 BsrBI CCG'CTC Cuts at: 1394 1823 5235 1394 Size: 429 3412 3507 Fragments arranged by size: 3507 3412 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5843 6017 65 Size: 1183 4595 174 1396 Fragments arranged by size: 4595 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2613 2940 3075 3237 5992 6179 1700 Size: 110 803 327 135 162 2755 187 2869 Fragments arranged by size: 2869 2755 803 327 187 162 135 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7348 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7348 BssSI C'ACGA_G Cuts at: 5408 6420 5408 Size: 1012 6336 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7348 BstBI TT'CG_AA Cuts at: 3903 6152 3903 Size: 2249 5099 BstEII G'GTnAC_C Cuts at: 1184 4576 1184 Size: 3392 3956 BstYI r'GATC_y Cuts at: 1972 2487 2973 2982 3015 3982 4678 5045 5430 Size: 515 486 9 33 967 696 367 385 Cuts at: 5430 5447 6899 6985 6997 7343 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 967 696 515 486 385 367 346 86 33 17 12 9 BstZ17I GTA'TAC Cuts at: 4594 4594 Size: 7348 BtgI C'CryG_G Cuts at: 513 1065 2113 3889 4248 4269 4335 513 Size: 552 1048 1776 359 21 66 3526 Fragments arranged by size: 3526 1776 1048 552 359 66 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3762 487 Size: 683 56 2536 4073 Fragments arranged by size: 4073 2536 683 56 BtsI GCAGTG_nn' Cuts at: 4398 4864 5658 5678 4398 Size: 466 794 20 6068 Fragments arranged by size: 6068 794 466 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7348 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7313 DraI TTT'AAA Cuts at: 4652 4652 Size: 7348 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3872 6355 809 Size: 507 2556 2483 1802 Fragments arranged by size: 2556 2483 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2116 2353 3801 Size: 54 1425 50 275 18 286 237 1448 Cuts at: 3801 4119 4332 4536 4795 5684 7313 8 Size: 318 213 204 259 889 1629 43 Fragments arranged by size: 1629 1448 1425 889 318 286 275 259 237 213 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4795 1537 Size: 275 2983 4090 Fragments arranged by size: 4090 2983 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5278 1353 Size: 3925 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5935 6309 6455 387 Size: 1064 138 4346 374 146 1280 Fragments arranged by size: 4346 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2722 3544 3931 2722 Size: 822 387 6139 Fragments arranged by size: 6139 822 387 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2774 3539 3578 3805 3806 3872 5411 Size: 393 1142 765 39 227 1 66 1539 Cuts at: 5411 6008 6795 7063 1239 Size: 597 787 268 1524 Fragments arranged by size: 1539 1524 1142 787 765 597 393 268 227 66 39 1 EcoNI CCTnn'n_nnAGG Cuts at: 2405 2992 2405 Size: 587 6761 EcoO109I rG'GnC_Cy Cuts at: 2021 2056 3198 3350 3552 3727 3964 3996 3997 Size: 35 1142 152 202 175 237 32 1 Cuts at: 3997 4050 4525 2021 Size: 53 475 4844 Fragments arranged by size: 4844 1142 475 237 202 175 152 53 35 32 1 EcoRI G'AATT_C Cuts at: 4657 5035 4657 Size: 378 6970 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2431 2431 Size: 7348 FspI TGC'GCA Cuts at: 5854 5854 Size: 7348 HaeII r_GCGC'y Cuts at: 1678 3228 3900 4398 6495 1678 Size: 1550 672 498 2097 2531 Fragments arranged by size: 2531 2097 1550 672 498 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3368 3400 4001 4033 4244 Size: 32 135 32 1796 32 601 32 211 Cuts at: 4244 4276 5989 6021 6063 6095 1373 Size: 32 1713 32 42 32 2626 Fragments arranged by size: 2626 1796 1713 601 211 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4692 4938 669 Size: 988 281 2754 246 3079 Fragments arranged by size: 3079 2754 988 281 246 HindIII A'AGCT_T Cuts at: 748 2840 748 Size: 2092 5256 HpaI GTT'AAC Cuts at: 4938 4938 Size: 7348 KasI G'GCGC_C Cuts at: 4394 4394 Size: 7348 KpnI G_GTAC'C Cuts at: 4673 4673 Size: 7348 MfeI C'AATT_G Cuts at: 3051 4947 3051 Size: 1896 5452 MluI A'CGCG_T Cuts at: 7224 7224 Size: 7348 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3455 6462 6646 1305 Size: 2150 3007 184 2007 Fragments arranged by size: 3007 2150 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3803 4121 4334 4538 10 Size: 54 1425 343 1971 318 213 204 2820 Fragments arranged by size: 2820 1971 1425 343 318 213 204 54 MslI CAynn'nnrTG Cuts at: 518 1166 2096 5306 5665 5824 518 Size: 648 930 3210 359 159 2042 Fragments arranged by size: 3210 2042 930 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2551 2590 4304 4764 5441 6589 6834 Size: 478 467 39 1714 460 677 1148 245 Cuts at: 6834 1606 Size: 2120 Fragments arranged by size: 2120 1714 1148 677 478 467 460 245 39 NaeI GCC'GGC Cuts at: 2615 2615 Size: 7348 NarI GG'CG_CC Cuts at: 4395 4395 Size: 7348 NcoI C'CATG_G Cuts at: 513 1065 3889 4269 513 Size: 552 2824 380 3592 Fragments arranged by size: 3592 2824 552 380 NdeI CA'TA_TG Cuts at: 387 387 Size: 7348 NgoMIV G'CCGG_C Cuts at: 2613 2613 Size: 7348 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7348 NotI GC'GGCC_GC Cuts at: 4795 4795 Size: 7348 NspI r_CATG'y Cuts at: 1132 1229 2642 2702 4706 6251 1132 Size: 97 1413 60 2004 1545 2229 Fragments arranged by size: 2229 2004 1545 1413 97 60 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7348 PciI A'CATG_T Cuts at: 6247 6247 Size: 7348 PflMI CCAn_nnn'nTGG Cuts at: 1108 4528 1108 Size: 3420 3928 PfoI T'CCnGG_A Cuts at: 4289 4289 Size: 7348 PmeI GTTT'AAAC Cuts at: 4652 4652 Size: 7348 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5417 5449 6930 6962 5417 Size: 32 1481 32 5803 Fragments arranged by size: 5803 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3198 3552 3198 Size: 354 6994 PshAI GACnn'nnGTC Cuts at: 1613 4163 1613 Size: 2550 4798 PsiI TTA'TAA Cuts at: 4918 4918 Size: 7348 PspOMI G'GGCC_C Cuts at: 3726 3996 3726 Size: 270 7078 PstI C_TGCA'G Cuts at: 830 2110 2589 4158 4560 4700 830 Size: 1280 479 1569 402 140 3478 Fragments arranged by size: 3478 1569 1280 479 402 140 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7348 PvuII CAG'CTG Cuts at: 2551 2590 4304 2551 Size: 39 1714 5595 Fragments arranged by size: 5595 1714 39 SacI G_AGCT'C Cuts at: 2724 3546 3933 2724 Size: 822 387 6139 Fragments arranged by size: 6139 822 387 SalI G'TCGA_C Cuts at: 1936 4690 1936 Size: 2754 4594 SbfI CC_TGCA'GG Cuts at: 2110 4700 2110 Size: 2590 4758 ScaI AGT'ACT Cuts at: 5596 7308 5596 Size: 1712 5636 SexAI A'CCwGG_T Cuts at: 2376 2376 Size: 7348 SfcI C'TryA_G Cuts at: 826 1045 2106 2206 2585 4154 4556 4696 5831 Size: 219 1061 100 379 1569 402 140 1135 Cuts at: 5831 6512 6703 826 Size: 681 191 1471 Fragments arranged by size: 1569 1471 1135 1061 681 402 379 219 191 140 100 SfoI GGC'GCC Cuts at: 4396 4396 Size: 7348 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7348 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7348 SmaI CCC'GGG Cuts at: 1872 2747 2802 3725 3980 4675 1872 Size: 875 55 923 255 695 4545 Fragments arranged by size: 4545 923 875 695 255 55 SmlI C'TyrA_G Cuts at: 820 1017 1943 2978 4283 5452 6353 6615 820 Size: 197 926 1035 1305 1169 901 262 1553 Fragments arranged by size: 1553 1305 1169 1035 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5139 493 Size: 4646 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7348 SphI G_CATG'C Cuts at: 1132 2642 4706 1132 Size: 1510 2064 3774 Fragments arranged by size: 3774 2064 1510 SspI AAT'ATT Cuts at: 5 52 5272 5 Size: 47 5220 2081 Fragments arranged by size: 5220 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7348 StyI C'CwwG_G Cuts at: 513 1065 2469 2874 3153 3645 3889 4269 4787 Size: 552 1404 405 279 492 244 380 518 Cuts at: 4787 513 Size: 3074 Fragments arranged by size: 3074 1404 552 518 492 405 380 279 244 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2136 2666 3166 3316 4199 4445 5375 Size: 1255 556 530 500 150 883 246 930 Cuts at: 5375 5392 5545 5730 6175 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 930 883 556 530 500 445 246 185 153 150 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5594 7306 96 Size: 274 80 33 51 1443 3617 1712 138 Fragments arranged by size: 3617 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2318 2350 7308 7340 2318 Size: 32 4958 32 2326 Fragments arranged by size: 4958 2326 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2378 1316 Size: 1062 6286 XbaI T'CTAG_A Cuts at: 4684 4684 Size: 7348 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3160 4193 3160 Size: 1033 6315 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7348 XmaI C'CCGG_G Cuts at: 1870 2745 2800 3723 3978 4673 1870 Size: 875 55 923 255 695 4545 Fragments arranged by size: 4545 923 875 695 255 55 XmnI GAAnn'nnTTC Cuts at: 5477 5477 Size: 7348 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5156 276 Size: 53 83 186 936 3622 2468 Fragments arranged by size: 3622 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI