(Circular) (Six-base) MAPSORT of: pFN21AA0309.seq Check: 2878 from: 1 to: 13852 pFN21AA0309 13852 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5107 9616 10030 5107 Size: 4509 414 8929 Fragments arranged by size: 8929 4509 414 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 11032 11662 278 Size: 53 83 186 936 9496 630 2468 Fragments arranged by size: 9496 2468 936 630 186 83 53 AccI GT'mk_AC Cuts at: 1937 6368 11195 1937 Size: 4431 4827 4594 Fragments arranged by size: 4827 4594 4431 Acc65I G'GTAC_C Cuts at: 6403 6634 11173 6403 Size: 231 4539 9082 Fragments arranged by size: 9082 4539 231 AclI AA'CG_TT Cuts at: 1563 3997 11979 12352 1563 Size: 2434 7982 373 3063 Fragments arranged by size: 7982 3063 2434 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2318 3051 3063 3540 4687 5607 5631 6795 Size: 686 733 12 477 1147 920 24 1164 Cuts at: 6795 7242 8160 8790 9393 9437 9561 11004 11915 Size: 447 918 630 603 44 124 1443 911 Cuts at: 11915 13299 13567 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1443 1384 1164 1147 920 918 911 733 686 630 603 477 447 268 124 44 24 12 AfeI AGC'GCT Cuts at: 4208 8402 4208 Size: 4194 9658 AflII C'TTAA_G Cuts at: 820 1017 7564 820 Size: 197 6547 7108 Fragments arranged by size: 7108 6547 197 AflIII A'CryG_T Cuts at: 1203 6058 12751 13728 1203 Size: 4855 6693 977 1327 Fragments arranged by size: 6693 4855 1327 977 AgeI A'CCGG_T Cuts at: 4666 6400 12683 4666 Size: 1734 6283 5835 Fragments arranged by size: 6283 5835 1734 AhdI GACnn_n'nnGTC Cuts at: 2674 6024 7366 12581 2674 Size: 3350 1342 5215 3945 Fragments arranged by size: 5215 3945 3350 1342 AleI CACnn'nnGTG Cuts at: 1166 3965 5570 9791 1166 Size: 2799 1605 4221 5227 Fragments arranged by size: 5227 4221 2799 1605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3303 8382 9809 1184 Size: 2119 5079 1427 5227 Fragments arranged by size: 5227 5079 2119 1427 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7158 7190 8504 8536 7158 Size: 32 1314 32 12474 Fragments arranged by size: 12474 1314 32 32 AlwNI CAG_nnn'CTG Cuts at: 3311 3431 3707 5024 6314 6502 6655 6697 6823 Size: 120 276 1317 1290 188 153 42 126 Cuts at: 6823 7856 7878 8849 9227 9341 9440 10079 10148 Size: 1033 22 971 378 114 99 639 69 Cuts at: 10148 10178 11595 13167 3311 Size: 30 1417 1572 3996 Fragments arranged by size: 3996 1572 1417 1317 1290 1033 971 639 378 276 188 153 126 120 114 99 69 42 30 22 ApaI G_GGCC'C Cuts at: 2195 2485 3381 3660 4997 5109 5870 6746 6860 Size: 290 896 279 1337 112 761 876 114 Cuts at: 6860 7201 9885 10209 10891 2195 Size: 341 2684 324 682 5156 Fragments arranged by size: 5156 2684 1337 896 876 761 682 341 324 290 279 114 112 ApaLI G'TGCA_C Cuts at: 11909 13065 11909 Size: 1156 12696 ApoI r'AATT_y Cuts at: 1493 8270 8414 11161 11387 11539 13648 1493 Size: 6777 144 2747 226 152 2109 1697 Fragments arranged by size: 6777 2747 2109 1697 226 152 144 AseI AT'TA_AT Cuts at: 160 1032 12406 160 Size: 872 11374 1606 Fragments arranged by size: 11374 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 13852 AvaI C'yCGr_G Cuts at: 1870 1943 3018 3246 5412 5544 6478 7509 7935 Size: 73 1075 228 2166 132 934 1031 426 Cuts at: 7935 8349 8667 9823 9983 10559 11177 1870 Size: 414 318 1156 160 576 618 4545 Fragments arranged by size: 4545 2166 1156 1075 1031 934 618 576 426 414 318 228 160 132 73 AvrII C'CTAG_G Cuts at: 8783 8783 Size: 13852 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6957 6990 6957 Size: 33 13819 BamHI G'GATC_C Cuts at: 11182 11549 11182 Size: 367 13485 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 2314 2448 4002 4833 Size: 325 217 627 345 182 134 1554 831 Cuts at: 4833 4984 5306 5902 5941 6109 6298 6403 6634 Size: 151 322 596 39 168 189 105 231 Cuts at: 6634 6664 6947 7204 7414 8591 9331 10099 11173 Size: 30 283 257 210 1177 740 768 1074 Cuts at: 11173 12628 618 Size: 1455 1842 Fragments arranged by size: 1842 1554 1455 1177 1074 831 768 740 627 596 345 325 322 283 257 231 217 210 189 182 168 151 134 105 39 30 BanII G_rGCy'C Cuts at: 1391 2195 2325 2485 3054 3381 3660 4039 4997 Size: 804 130 160 569 327 279 379 958 Cuts at: 4997 5031 5109 5468 5551 5870 6485 6746 6836 Size: 34 78 359 83 319 615 261 90 Cuts at: 6836 6860 7201 7503 7516 8457 8668 9423 9885 Size: 24 341 302 13 941 211 755 462 Cuts at: 9885 10209 10891 11171 1391 Size: 324 682 280 4072 Fragments arranged by size: 4072 958 941 804 755 682 615 569 462 379 359 341 327 324 319 302 280 279 261 211 160 130 90 83 78 34 24 13 BbeI G_GCGC'C Cuts at: 4988 4988 Size: 13852 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3887 4315 6000 8648 928 Size: 971 1988 428 1685 2648 6132 Fragments arranged by size: 6132 2648 1988 1685 971 428 BbvCI CC'TCA_GC Cuts at: 2743 2958 3192 6391 8331 9345 9430 9549 2743 Size: 215 234 3199 1940 1014 85 119 7046 Fragments arranged by size: 7046 3199 1940 1014 234 215 119 85 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 10807 10841 12043 12077 1903 Size: 34 8870 34 1202 34 3678 Fragments arranged by size: 8870 3678 1202 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3172 6985 11744 12954 3172 Size: 3813 4759 1210 4070 Fragments arranged by size: 4759 4070 3813 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 13852 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3914 5225 7269 Size: 107 122 71 631 605 2242 1311 2044 Cuts at: 7269 7331 8237 9718 12463 136 Size: 62 906 1481 2745 1525 Fragments arranged by size: 2745 2242 2044 1525 1481 1311 906 631 605 122 107 71 62 BglII A'GATC_T Cuts at: 9211 13847 9211 Size: 4636 9216 BlpI GC'TnA_GC Cuts at: 2268 3233 8259 8355 11269 2268 Size: 965 5026 96 2914 4851 Fragments arranged by size: 5026 4851 2914 965 96 Bme1580I G_kGCm'C Cuts at: 1790 2145 2195 2485 3381 3660 4007 4997 5109 Size: 355 50 290 896 279 347 990 112 Cuts at: 5109 5311 5870 6746 6860 7201 8130 9066 9127 Size: 202 559 876 114 341 929 936 61 Cuts at: 9127 9204 9885 10209 10891 11913 13069 1790 Size: 77 681 324 682 1022 1156 2573 Fragments arranged by size: 2573 1156 1022 990 936 929 896 876 682 681 559 355 347 341 324 290 279 202 114 112 77 61 50 BmgBI CAC'GTC Cuts at: 1318 7466 1318 Size: 6148 7704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2353 3943 3988 Size: 416 53 83 399 24 936 1590 45 Cuts at: 3988 4997 5048 6689 6836 7546 8837 8849 10136 Size: 1009 51 1641 147 710 1291 12 1287 Cuts at: 10136 10886 11583 12541 442 Size: 750 697 958 1753 Fragments arranged by size: 1753 1641 1590 1291 1287 1009 958 936 750 710 697 416 399 147 83 53 51 45 24 12 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 13852 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4372 8471 10022 10120 4372 Size: 4099 1551 98 8104 Fragments arranged by size: 8104 4099 1551 98 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2100 2168 2572 2689 2737 3636 3701 4733 Size: 861 68 404 117 48 899 65 1032 Cuts at: 4733 5001 6144 6168 6249 6357 6603 6627 6693 Size: 268 1143 24 81 108 246 24 66 Cuts at: 6693 6711 6720 6759 7148 8123 8204 8441 8453 Size: 18 9 39 389 975 81 237 12 Cuts at: 8453 8784 8988 9126 9173 9437 9485 9499 10053 Size: 331 204 138 47 264 48 14 554 Cuts at: 10053 12512 1239 Size: 2459 2579 Fragments arranged by size: 2579 2459 1143 1032 975 899 861 554 404 389 331 268 264 246 237 204 138 117 108 81 81 68 66 65 48 48 47 39 24 24 18 14 12 9 Bpu10I CC'TnA_GC Cuts at: 2391 2743 2958 3192 4724 4941 5025 6165 6391 Size: 352 215 234 1532 217 84 1140 226 Cuts at: 6391 7899 8331 9345 9430 9549 2391 Size: 1508 432 1014 85 119 6694 Fragments arranged by size: 6694 1532 1508 1140 1014 432 352 234 226 217 215 119 85 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2076 2618 4040 5570 6850 7610 8664 9121 11977 Size: 542 1422 1530 1280 760 1054 457 2856 Cuts at: 11977 12842 13140 2076 Size: 865 298 2788 Fragments arranged by size: 2856 2788 1530 1422 1280 1054 865 760 542 457 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 9808 10071 10151 10798 10842 12515 882 Size: 617 8309 263 80 647 44 1673 2219 Fragments arranged by size: 8309 2219 1673 647 617 263 80 44 BsaAI yAC'GTr Cuts at: 493 1204 5668 11643 493 Size: 711 4464 5975 2702 Fragments arranged by size: 5975 4464 2702 711 BsaBI GATnn'nnATC Cuts at: 4740 13846 4740 Size: 9106 4746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3758 4243 4985 7081 Size: 53 83 186 936 2225 485 742 2096 Cuts at: 7081 9625 11029 11659 12041 12676 275 Size: 2544 1404 630 382 635 1451 Fragments arranged by size: 2544 2225 2096 1451 1404 936 742 635 630 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3441 4666 6289 6343 6400 12285 12683 12957 13104 Size: 1225 1623 54 57 5885 398 274 147 Cuts at: 13104 13515 3441 Size: 411 3778 Fragments arranged by size: 5885 3778 1623 1225 411 398 274 147 57 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3974 4004 4233 4246 4263 Size: 30 165 30 2571 30 229 13 17 Cuts at: 4263 4276 4277 4307 6757 6787 7810 7840 8504 Size: 13 1 30 2450 30 1023 30 664 Cuts at: 8504 8534 8950 8980 8986 9016 1178 Size: 30 416 30 6 30 6014 Fragments arranged by size: 6014 2571 2450 1023 664 416 229 165 30 30 30 30 30 30 30 30 30 17 13 13 6 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2471 2507 3284 3390 3488 3578 4211 5004 Size: 1285 36 777 106 98 90 633 793 Cuts at: 5004 5649 5710 6261 6264 6273 7994 8159 8354 Size: 645 61 551 3 9 1721 165 195 Cuts at: 8354 8450 8465 8528 8552 8555 8558 8904 8955 Size: 96 15 63 24 3 3 346 51 Cuts at: 8955 9027 9175 9395 9609 10985 11012 11015 1186 Size: 72 148 220 214 1376 27 3 4023 Fragments arranged by size: 4023 1721 1376 1285 793 777 645 633 551 346 220 214 195 165 148 106 98 96 90 72 63 61 51 36 27 24 15 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2047 4007 5037 5446 5809 5869 5914 6088 6514 Size: 1960 1030 409 363 60 45 174 426 Cuts at: 6514 6745 6751 6805 6889 6907 7034 7289 7347 Size: 231 6 54 84 18 127 255 58 Cuts at: 7347 8558 9281 9924 9958 10079 10209 10224 10517 Size: 1211 723 643 34 121 130 15 293 Cuts at: 10517 13055 13551 2047 Size: 2538 496 2348 Fragments arranged by size: 2538 2348 1960 1211 1030 723 643 496 426 409 363 293 255 231 174 130 127 121 84 60 58 54 45 34 18 15 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 6836 8247 8941 9261 1163 Size: 573 5100 1411 694 320 5754 Fragments arranged by size: 5754 5100 1411 694 573 320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4667 6864 11302 12063 13091 1540 Size: 275 186 2666 2197 4438 761 1028 2301 Fragments arranged by size: 4438 2666 2301 2197 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5031 5468 7503 7650 8457 9423 10003 11913 11998 Size: 437 2035 147 807 966 580 1910 85 Cuts at: 11998 13069 5031 Size: 1071 5814 Fragments arranged by size: 5814 2035 1910 1071 966 807 580 437 147 85 BsmI GAATG_Cn' Cuts at: 3852 7677 10692 11363 11456 3852 Size: 3825 3015 671 93 6248 Fragments arranged by size: 6248 3825 3015 671 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4234 9987 4234 Size: 5753 8099 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2195 2325 2485 3054 3381 3660 Size: 399 355 50 130 160 569 327 279 Cuts at: 3660 4007 4039 4997 5031 5109 5311 5468 5551 Size: 347 32 958 34 78 202 157 83 Cuts at: 5551 5870 6485 6746 6836 6860 7201 7503 7516 Size: 319 615 261 90 24 341 302 13 Cuts at: 7516 7650 8130 8457 8668 9066 9127 9204 9423 Size: 134 480 327 211 398 61 77 219 Cuts at: 9423 9885 10003 10209 10891 11171 11913 11998 13069 Size: 462 118 206 682 280 742 85 1071 Cuts at: 13069 1391 Size: 2174 Fragments arranged by size: 2174 1071 958 742 682 615 569 480 462 399 398 355 347 341 327 327 319 302 280 279 261 219 211 206 202 160 157 134 130 118 90 85 83 78 77 61 50 34 32 24 13 BspEI T'CCGG_A Cuts at: 13515 13515 Size: 13852 BspHI T'CATG_A Cuts at: 3004 4596 11741 13471 3004 Size: 1592 7145 1730 3385 Fragments arranged by size: 7145 3385 1730 1592 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2634 5107 7728 9616 10030 11207 844 Size: 1790 2473 2621 1888 414 1177 3489 Fragments arranged by size: 3489 2621 2473 1888 1790 1177 414 BsrBI CCG'CTC Cuts at: 1394 1823 6953 11739 1394 Size: 429 5130 4786 3507 Fragments arranged by size: 5130 4786 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2563 2875 3693 7016 9415 9936 12347 Size: 1183 1315 312 818 3323 2399 521 2411 Cuts at: 12347 12521 65 Size: 174 1396 Fragments arranged by size: 3323 2411 2399 1396 1315 1183 818 521 312 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4666 5514 6400 8319 8598 11043 12496 Size: 110 2856 848 886 1919 279 2445 1453 Cuts at: 12496 12683 1700 Size: 187 2869 Fragments arranged by size: 2869 2856 2445 1919 1453 886 848 279 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 13852 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 13852 BssSI C'ACGA_G Cuts at: 2453 8640 10296 11912 12924 2453 Size: 6187 1656 1616 1012 3381 Fragments arranged by size: 6187 3381 1656 1616 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6194 6947 7797 8825 794 Size: 5400 753 850 1028 5821 Fragments arranged by size: 5821 5400 1028 850 753 BstBI TT'CG_AA Cuts at: 9703 12656 9703 Size: 2953 10899 BstEII G'GTnAC_C Cuts at: 1184 3823 4669 6026 1184 Size: 2639 846 1357 9010 Fragments arranged by size: 9010 2639 1357 846 BstXI CCAn_nnnn'nTGG Cuts at: 3806 6220 7452 10217 10720 3806 Size: 2414 1232 2765 503 6938 Fragments arranged by size: 6938 2765 2414 1232 503 BstYI r'GATC_y Cuts at: 1972 7733 9211 9234 10174 11182 11549 11934 11951 Size: 5761 1478 23 940 1008 367 385 17 Cuts at: 11951 13403 13489 13501 13847 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 5761 1977 1478 1452 1008 940 385 367 346 86 23 17 12 Bsu36I CC'TnA_GG Cuts at: 2310 3057 3402 9108 9594 10956 10990 2310 Size: 747 345 5706 486 1362 34 5172 Fragments arranged by size: 5706 5172 1362 747 486 345 34 BtgI C'CryG_G Cuts at: 513 1065 2526 3225 3264 3381 4731 4979 5364 Size: 552 1461 699 39 117 1350 248 385 Cuts at: 5364 6081 6969 7705 7890 10713 513 Size: 717 888 736 185 2823 3652 Fragments arranged by size: 3652 2823 1461 1350 888 736 717 699 552 385 248 185 117 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4319 6288 487 Size: 683 56 3093 1969 8051 Fragments arranged by size: 8051 3093 1969 683 56 BtsI GCAGTG_nn' Cuts at: 2375 3325 3643 5156 7047 7614 9908 10001 10400 Size: 950 318 1513 1891 567 2294 93 399 Cuts at: 10400 10416 11368 12162 12182 2375 Size: 16 952 794 20 4045 Fragments arranged by size: 4045 2294 1891 1513 952 950 794 567 399 318 93 20 16 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 13852 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3899 3934 529 Size: 35 3335 35 10447 Fragments arranged by size: 10447 3335 35 35 DraI TTT'AAA Cuts at: 4015 11156 4015 Size: 7141 6711 DraIII CAC_nnn'GTG Cuts at: 7645 7645 Size: 13852 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 12859 809 Size: 507 11543 1802 Fragments arranged by size: 11543 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2529 3738 5512 Size: 54 1425 50 275 18 699 1209 1774 Cuts at: 5512 5988 6126 6861 7708 7927 8028 8160 8317 Size: 476 138 735 847 219 101 132 157 Cuts at: 8317 9168 9671 11299 12188 13817 8 Size: 851 503 1628 889 1629 43 Fragments arranged by size: 1774 1629 1628 1425 1209 889 851 847 735 699 503 476 275 219 157 138 132 101 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6861 11299 1537 Size: 275 5049 4438 4090 Fragments arranged by size: 5049 4438 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 2294 2355 2725 2743 2817 2928 3042 Size: 848 93 61 370 18 74 111 114 Cuts at: 3042 3132 3150 3168 3387 3516 3555 3606 5586 Size: 90 18 18 219 129 39 51 1980 Cuts at: 5586 5659 5701 6058 6291 8003 8136 8145 8208 Size: 73 42 357 233 1712 133 9 63 Cuts at: 8208 8463 9480 10472 10694 11782 1353 Size: 255 1017 992 222 1088 3423 Fragments arranged by size: 3423 1980 1712 1088 1017 992 848 370 357 255 233 222 219 133 129 114 111 93 90 74 73 63 61 51 42 39 18 18 18 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8513 8698 12439 12813 12959 387 Size: 1064 138 6924 185 3741 374 146 1280 Fragments arranged by size: 6924 3741 1280 1064 374 185 146 138 EcoICRI GAG'CTC Cuts at: 5029 5466 7501 8455 9421 5029 Size: 437 2035 954 966 9460 Fragments arranged by size: 9460 2035 966 954 437 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2168 2318 2572 2689 2737 3051 Size: 393 468 68 150 254 117 48 314 Cuts at: 3051 3063 3540 3636 3701 4687 4733 5001 5607 Size: 12 477 96 65 986 46 268 606 Cuts at: 5607 5631 6144 6168 6249 6357 6603 6627 6693 Size: 24 513 24 81 108 246 24 66 Cuts at: 6693 6711 6720 6759 6795 7148 7242 8123 8160 Size: 18 9 39 36 353 94 881 37 Cuts at: 8160 8204 8441 8453 8784 8790 8988 9126 9173 Size: 44 237 12 331 6 198 138 47 Cuts at: 9173 9393 9437 9485 9499 9561 10053 11004 11915 Size: 220 44 48 14 62 492 951 911 Cuts at: 11915 12512 13299 13567 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 986 951 911 881 787 606 597 513 492 477 468 393 353 331 314 268 268 254 246 237 220 198 150 138 117 108 96 94 81 68 66 65 62 48 48 47 46 44 44 39 37 36 24 24 24 18 14 12 12 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 3033 3651 4424 5016 7994 3033 Size: 618 773 592 2978 8891 Fragments arranged by size: 8891 2978 773 618 592 EcoO109I rG'GnC_Cy Cuts at: 2120 2191 2972 3377 3656 3938 4994 5021 5106 Size: 71 781 405 279 282 1056 27 85 Cuts at: 5106 6587 7197 7198 9477 9599 9689 9882 10047 Size: 1481 610 1 2279 122 90 193 165 Cuts at: 10047 10206 10887 10888 10952 11016 2120 Size: 159 681 1 64 64 4956 Fragments arranged by size: 4956 2279 1481 1056 781 681 610 405 282 279 193 165 159 122 90 85 71 64 64 27 1 1 EcoRI G'AATT_C Cuts at: 11161 11539 11161 Size: 378 13474 EcoRV GAT'ATC Cuts at: 8221 8221 Size: 13852 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3275 3275 Size: 13852 FseI GG_CCGG'CC Cuts at: 8323 8323 Size: 13852 FspI TGC'GCA Cuts at: 2435 7547 11087 12358 2435 Size: 5112 3540 1271 3929 Fragments arranged by size: 5112 3929 3540 1271 FspAI rTGC'GCAy Cuts at: 2435 7547 2435 Size: 5112 8740 HaeII r_GCGC'y Cuts at: 1678 2401 4159 4210 4988 6884 8404 11097 12999 Size: 723 1758 51 778 1896 1520 2693 1902 Cuts at: 12999 1678 Size: 2531 Fragments arranged by size: 2693 2531 1902 1896 1758 1520 778 723 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2242 2274 2660 2692 3524 Size: 32 135 32 670 32 386 32 832 Cuts at: 3524 3556 3883 3915 4233 4246 4265 4277 4278 Size: 32 327 32 318 13 19 12 1 Cuts at: 4278 4309 6010 6042 6685 6717 6757 6789 7352 Size: 31 1701 32 643 32 40 32 563 Cuts at: 7352 7384 7635 7667 8330 8362 9464 9496 10328 Size: 32 251 32 663 32 1102 32 832 Cuts at: 10328 10360 12493 12525 12567 12599 1373 Size: 32 2133 32 42 32 2626 Fragments arranged by size: 2626 2133 1701 1102 832 832 670 663 643 563 386 327 318 251 135 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 31 19 13 12 1 HincII GTy'rAC Cuts at: 669 1657 1938 4529 4918 5505 5880 11196 11442 Size: 988 281 2591 389 587 375 5316 246 Cuts at: 11442 669 Size: 3079 Fragments arranged by size: 5316 3079 2591 988 587 389 375 281 246 HindIII A'AGCT_T Cuts at: 748 3842 748 Size: 3094 10758 HpaI GTT'AAC Cuts at: 5505 11442 5505 Size: 5937 7915 KasI G'GCGC_C Cuts at: 4984 4984 Size: 13852 KpnI G_GTAC'C Cuts at: 6407 6638 11177 6407 Size: 231 4539 9082 Fragments arranged by size: 9082 4539 231 MfeI C'AATT_G Cuts at: 7487 11451 7487 Size: 3964 9888 MluI A'CGCG_T Cuts at: 13728 13728 Size: 13852 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2384 2946 3045 3183 6437 6620 6710 7201 Size: 1079 562 99 138 3254 183 90 491 Cuts at: 7201 10016 10334 10752 12966 13150 1305 Size: 2815 318 418 2214 184 2007 Fragments arranged by size: 3254 2815 2214 2007 1079 562 491 418 318 184 183 138 99 90 MscI TGG'CCA Cuts at: 10 64 1489 1832 2531 3740 5990 6128 7710 Size: 54 1425 343 699 1209 2250 138 1582 Cuts at: 7710 7929 8030 8162 9170 10 Size: 219 101 132 1008 4692 Fragments arranged by size: 4692 2250 1582 1425 1209 1008 699 343 219 138 132 101 54 MslI CAynn'nnrTG Cuts at: 518 1166 3009 3123 3965 4616 5570 7661 9791 Size: 648 1843 114 842 651 954 2091 2130 Cuts at: 9791 10718 11810 12169 12328 518 Size: 927 1092 359 159 2042 Fragments arranged by size: 2130 2091 2042 1843 1092 954 927 842 651 648 359 159 114 MspA1I CmG'CkG Cuts at: 1606 2464 2747 2962 3191 3707 4509 4624 4753 Size: 858 283 215 229 516 802 115 129 Cuts at: 4753 5347 5480 5918 7150 7366 7914 8386 8745 Size: 594 133 438 1232 216 548 472 359 Cuts at: 8745 9548 9772 11268 11945 13093 13338 1606 Size: 803 224 1496 677 1148 245 2120 Fragments arranged by size: 2120 1496 1232 1148 858 803 802 677 594 548 516 472 438 359 283 245 229 224 216 215 133 129 115 NaeI GCC'GGC Cuts at: 5516 8321 11045 5516 Size: 2805 2724 8323 Fragments arranged by size: 8323 2805 2724 NarI GG'CG_CC Cuts at: 4985 4985 Size: 13852 NcoI C'CATG_G Cuts at: 513 1065 2526 3225 3264 5364 6969 7705 7890 Size: 552 1461 699 39 2100 1605 736 185 Cuts at: 7890 10713 513 Size: 2823 3652 Fragments arranged by size: 3652 2823 2100 1605 1461 736 699 552 185 39 NdeI CA'TA_TG Cuts at: 387 387 Size: 13852 NgoMIV G'CCGG_C Cuts at: 5514 8319 11043 5514 Size: 2805 2724 8323 Fragments arranged by size: 8323 2805 2724 NheI G'CTAG_C Cuts at: 1052 1052 Size: 13852 NotI GC'GGCC_GC Cuts at: 11299 11299 Size: 13852 NruI TCG'CGA Cuts at: 4338 4338 Size: 13852 NspI r_CATG'y Cuts at: 1132 1229 4502 6062 6096 7436 7546 9850 11210 Size: 97 3273 1560 34 1340 110 2304 1360 Cuts at: 11210 12755 1132 Size: 1545 2229 Fragments arranged by size: 3273 2304 2229 1560 1545 1360 1340 110 97 34 PasI CC'CwG_GG Cuts at: 1348 4990 5684 6583 6853 10148 10313 10594 10948 Size: 3642 694 899 270 3295 165 281 354 Cuts at: 10948 1348 Size: 4252 Fragments arranged by size: 4252 3642 3295 899 694 354 281 270 165 PciI A'CATG_T Cuts at: 6058 12751 6058 Size: 6693 7159 PflMI CCAn_nnn'nTGG Cuts at: 1108 3311 7305 9133 10148 10898 1108 Size: 2203 3994 1828 1015 750 4062 Fragments arranged by size: 4062 3994 2203 1828 1015 750 PfoI T'CCnGG_A Cuts at: 3789 5923 6373 7227 7479 8418 10182 3789 Size: 2134 450 854 252 939 1764 7459 Fragments arranged by size: 7459 2134 1764 939 854 450 252 PmeI GTTT'AAAC Cuts at: 11156 11156 Size: 13852 PmlI CAC'GTG Cuts at: 5668 5668 Size: 13852 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8504 8536 11921 11953 13434 13466 8504 Size: 32 3385 32 1481 32 8890 Fragments arranged by size: 8890 3385 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3938 5021 6587 9477 9599 11016 3938 Size: 1083 1566 2890 122 1417 6774 Fragments arranged by size: 6774 2890 1566 1417 1083 122 PshAI GACnn'nnGTC Cuts at: 1613 4764 7223 7914 8642 1613 Size: 3151 2459 691 728 6823 Fragments arranged by size: 6823 3151 2459 728 691 PsiI TTA'TAA Cuts at: 11422 11422 Size: 13852 PspOMI G'GGCC_C Cuts at: 2191 2481 3377 3656 4993 5105 5866 6742 6856 Size: 290 896 279 1337 112 761 876 114 Cuts at: 6856 7197 9881 10205 10887 2191 Size: 341 2684 324 682 5156 Fragments arranged by size: 5156 2684 1337 896 876 761 682 341 324 290 279 114 112 PspXI vC'TCGA_Gb Cuts at: 5544 5544 Size: 13852 PstI C_TGCA'G Cuts at: 830 3703 4125 4514 4932 5163 8750 10333 11204 Size: 2873 422 389 418 231 3587 1583 871 Cuts at: 11204 830 Size: 3478 Fragments arranged by size: 3587 3478 2873 1583 871 422 418 389 231 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 13852 PvuII CAG'CTG Cuts at: 2747 2962 3191 3707 4624 5480 7366 8386 9548 Size: 215 229 516 917 856 1886 1020 1162 Cuts at: 9548 2747 Size: 7051 Fragments arranged by size: 7051 1886 1162 1020 917 856 516 229 215 SacI G_AGCT'C Cuts at: 5031 5468 7503 8457 9423 5031 Size: 437 2035 954 966 9460 Fragments arranged by size: 9460 2035 966 954 437 SalI G'TCGA_C Cuts at: 1936 11194 1936 Size: 9258 4594 SanDI GG'GwC_CC Cuts at: 3938 6587 11016 3938 Size: 2649 4429 6774 Fragments arranged by size: 6774 4429 2649 SapI GCTCTTCn'nnn_ Cuts at: 2201 2725 2743 2928 3042 3387 3606 8463 9480 Size: 524 18 185 114 345 219 4857 1017 Cuts at: 9480 2201 Size: 6573 Fragments arranged by size: 6573 4857 1017 524 345 219 185 114 18 SbfI CC_TGCA'GG Cuts at: 11204 11204 Size: 13852 ScaI AGT'ACT Cuts at: 2663 3593 12100 13812 2663 Size: 930 8507 1712 2703 Fragments arranged by size: 8507 2703 1712 930 SexAI A'CCwGG_T Cuts at: 6022 6022 Size: 13852 SfcI C'TryA_G Cuts at: 826 1045 3183 3699 4121 4510 4928 5159 5748 Size: 219 2138 516 422 389 418 231 589 Cuts at: 5748 8746 10329 10931 11200 12335 13016 13207 826 Size: 2998 1583 602 269 1135 681 191 1471 Fragments arranged by size: 2998 2138 1583 1471 1135 681 602 589 516 422 418 389 269 231 219 191 SfoI GGC'GCC Cuts at: 4986 4986 Size: 13852 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 13852 SgrAI Cr'CCGG_yG Cuts at: 1700 8598 1700 Size: 6898 6954 SmaI CCC'GGG Cuts at: 1872 7511 7937 8351 8669 9825 11179 1872 Size: 5639 426 414 318 1156 1354 4545 Fragments arranged by size: 5639 4545 1354 1156 426 414 318 SmlI C'TyrA_G Cuts at: 820 1017 1943 2091 2597 3246 4055 5544 5585 Size: 197 926 148 506 649 809 1489 41 Cuts at: 5585 6829 7564 7625 8679 9100 10559 11956 12857 Size: 1244 735 61 1054 421 1459 1397 901 Cuts at: 12857 13119 820 Size: 262 1553 Fragments arranged by size: 1553 1489 1459 1397 1244 1054 926 901 809 735 649 506 421 262 197 148 61 41 SnaBI TAC'GTA Cuts at: 493 11643 493 Size: 11150 2702 SpeI A'CTAG_T Cuts at: 152 9812 152 Size: 9660 4192 SphI G_CATG'C Cuts at: 1132 6096 7436 11210 1132 Size: 4964 1340 3774 3774 Fragments arranged by size: 4964 3774 3774 1340 SrfI GCCC'GGGC Cuts at: 8669 8669 Size: 13852 SspI AAT'ATT Cuts at: 5 52 11776 5 Size: 47 11724 2081 Fragments arranged by size: 11724 2081 47 StuI AGG'CCT Cuts at: 1517 4136 4438 5578 7760 8426 8788 9106 1517 Size: 2619 302 1140 2182 666 362 318 6263 Fragments arranged by size: 6263 2619 2182 1140 666 362 318 302 StyI C'CwwG_G Cuts at: 513 1065 2526 2532 3113 3225 3264 3723 5364 Size: 552 1461 6 581 112 39 459 1641 Cuts at: 5364 5937 6876 6969 6993 7451 7705 7890 8241 Size: 573 939 93 24 458 254 185 351 Cuts at: 8241 8508 8783 9093 9291 10600 10713 11065 11291 Size: 267 275 310 198 1309 113 352 226 Cuts at: 11291 513 Size: 3074 Fragments arranged by size: 3074 1641 1461 1309 939 581 573 552 459 458 352 351 310 275 267 254 226 198 185 113 112 93 39 24 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5578 6541 7458 7565 7673 8249 8609 Size: 1255 3998 963 917 107 108 576 360 Cuts at: 8609 9295 10389 10483 11879 11896 12049 12234 12679 Size: 686 1094 94 1396 17 153 185 445 Cuts at: 12679 325 Size: 1498 Fragments arranged by size: 3998 1498 1396 1255 1094 963 917 686 576 445 360 185 153 108 107 94 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2661 3591 7336 Size: 274 80 33 51 1443 684 930 3745 Cuts at: 7336 12098 13810 96 Size: 4762 1712 138 Fragments arranged by size: 4762 3745 1712 1443 930 684 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2339 2371 2398 2430 5657 5689 5785 5814 5817 Size: 32 27 32 3227 32 96 29 3 Cuts at: 5817 5846 5895 5927 7810 7842 8950 8982 13812 Size: 29 49 32 1883 32 1108 32 4830 Cuts at: 13812 13844 2339 Size: 32 2347 Fragments arranged by size: 4830 3227 2347 1883 1108 96 49 32 32 32 32 32 32 32 29 29 27 3 Tth111I GACn'n_nGTC Cuts at: 1316 6607 6655 6697 10142 1316 Size: 5291 48 42 3445 5026 Fragments arranged by size: 5291 5026 3445 48 42 XbaI T'CTAG_A Cuts at: 11188 11188 Size: 13852 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2143 5045 5530 5566 8416 9289 9640 2143 Size: 2902 485 36 2850 873 351 6355 Fragments arranged by size: 6355 2902 2850 873 485 351 36 XhoI C'TCGA_G Cuts at: 1943 3246 5544 10559 1943 Size: 1303 2298 5015 5236 Fragments arranged by size: 5236 5015 2298 1303 XmaI C'CCGG_G Cuts at: 1870 7509 7935 8349 8667 9823 11177 1870 Size: 5639 426 414 318 1156 1354 4545 Fragments arranged by size: 5639 4545 1354 1156 426 414 318 XmnI GAAnn'nnTTC Cuts at: 2518 11981 2518 Size: 9463 4389 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 11030 11660 276 Size: 53 83 186 936 9496 630 2468 Fragments arranged by size: 9496 2468 936 630 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BfrBI BsiWI BstZ17I NsiI PacI PsrI RsrII SacII SfiI SwaI