(Circular) (Six-base) MAPSORT of: pFN21AA0294.seq Check: 7611 from: 1 to: 8737 pFN21AA0294 8737 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5364 5364 Size: 8737 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6547 278 Size: 53 83 186 936 5011 2468 Fragments arranged by size: 5011 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3219 5146 6080 1937 Size: 1282 1927 934 4594 Fragments arranged by size: 4594 1927 1282 934 Acc65I G'GTAC_C Cuts at: 6058 6058 Size: 8737 AclI AA'CG_TT Cuts at: 1563 6864 7237 1563 Size: 5301 373 3063 Fragments arranged by size: 5301 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2981 3341 3683 3686 4037 4955 5879 6800 Size: 1349 360 342 3 351 918 924 921 Cuts at: 6800 8184 8452 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1349 924 921 918 360 351 342 268 3 AfeI AGC'GCT Cuts at: 2338 2338 Size: 8737 AflII C'TTAA_G Cuts at: 820 1017 3248 820 Size: 197 2231 6309 Fragments arranged by size: 6309 2231 197 AflIII A'CryG_T Cuts at: 1203 3524 4039 5163 7636 8613 1203 Size: 2321 515 1124 2473 977 1327 Fragments arranged by size: 2473 2321 1327 1124 977 515 AgeI A'CCGG_T Cuts at: 7568 7568 Size: 8737 AhdI GACnn_n'nnGTC Cuts at: 7466 7466 Size: 8737 AleI CACnn'nnGTG Cuts at: 1166 2231 2327 3503 1166 Size: 1065 96 1176 6400 Fragments arranged by size: 6400 1176 1065 96 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8737 AlwNI CAG_nnn'CTG Cuts at: 2675 5032 5413 6480 8052 2675 Size: 2357 381 1067 1572 3360 Fragments arranged by size: 3360 2357 1572 1067 381 ApaLI G'TGCA_C Cuts at: 5957 6794 7950 5957 Size: 837 1156 6744 Fragments arranged by size: 6744 1156 837 ApoI r'AATT_y Cuts at: 1493 2762 3109 3551 4359 4753 5567 6046 6272 Size: 1269 347 442 808 394 814 479 226 Cuts at: 6272 6424 8533 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1269 814 808 479 442 394 347 226 152 AseI AT'TA_AT Cuts at: 160 1032 7291 160 Size: 872 6259 1606 Fragments arranged by size: 6259 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8737 AvaI C'yCGr_G Cuts at: 1870 1943 3164 4376 4862 5639 6062 1870 Size: 73 1221 1212 486 777 423 4545 Fragments arranged by size: 4545 1221 1212 777 486 423 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4160 4193 4160 Size: 33 8704 BamHI G'GATC_C Cuts at: 5087 5863 6067 6434 5087 Size: 776 204 367 7390 Fragments arranged by size: 7390 776 367 204 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2383 2407 2996 4092 4849 Size: 325 217 627 596 24 589 1096 757 Cuts at: 4849 5403 5459 5995 6058 7513 618 Size: 554 56 536 63 1455 1842 Fragments arranged by size: 1842 1455 1096 757 627 596 589 554 536 325 217 63 56 24 BanII G_rGCy'C Cuts at: 1391 2022 3723 5080 6056 1391 Size: 631 1701 1357 976 4072 Fragments arranged by size: 4072 1701 1357 976 631 BbeI G_GCGC'C Cuts at: 5999 5999 Size: 8737 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3329 3424 3919 4533 5166 5185 5661 Size: 971 1430 95 495 614 633 19 476 Cuts at: 5661 928 Size: 4004 Fragments arranged by size: 4004 1430 971 633 614 495 476 95 19 BbvCI CC'TCA_GC Cuts at: 2340 2340 Size: 8737 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6928 6962 1903 Size: 34 4991 34 3678 Fragments arranged by size: 4991 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6629 7839 6629 Size: 1210 7527 BclI T'GATC_A Cuts at: 1548 3766 5115 1548 Size: 2218 1349 5170 Fragments arranged by size: 5170 2218 1349 BfrBI ATG'CAT Cuts at: 3470 3470 Size: 8737 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2591 5918 7348 Size: 107 122 71 631 605 919 3327 1430 Cuts at: 7348 136 Size: 1525 Fragments arranged by size: 3327 1525 1430 919 631 605 122 107 71 BglII A'GATC_T Cuts at: 3086 8732 3086 Size: 5646 3091 BlpI GC'TnA_GC Cuts at: 6154 6154 Size: 8737 Bme1580I G_kGCm'C Cuts at: 1790 2334 2370 2412 4852 5406 5961 6798 7954 Size: 544 36 42 2440 554 555 837 1156 Cuts at: 7954 1790 Size: 2573 Fragments arranged by size: 2573 2440 1156 837 555 554 544 42 36 BmgBI CAC'GTC Cuts at: 1318 2469 5022 5498 1318 Size: 1151 2553 476 4557 Fragments arranged by size: 4557 2553 1151 476 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2225 3215 4043 Size: 416 53 83 399 24 808 990 828 Cuts at: 4043 6468 7426 442 Size: 2425 958 1753 Fragments arranged by size: 2425 1753 990 958 828 808 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8737 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3424 5817 3424 Size: 2393 6344 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2190 2235 2428 3032 3635 5255 5794 7397 Size: 951 45 193 604 603 1620 539 1603 Cuts at: 7397 1239 Size: 2579 Fragments arranged by size: 2579 1620 1603 951 604 603 539 193 45 Bpu10I CC'TnA_GC Cuts at: 2340 2942 5792 2340 Size: 602 2850 5285 Fragments arranged by size: 5285 2850 602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3116 3500 5333 6862 7727 8025 3116 Size: 384 1833 1529 865 298 3828 Fragments arranged by size: 3828 1833 1529 865 384 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2363 4859 5542 7400 882 Size: 617 864 2496 683 1858 2219 Fragments arranged by size: 2496 2219 1858 864 683 617 BsaAI yAC'GTr Cuts at: 493 1204 3238 6528 493 Size: 711 2034 3290 2702 Fragments arranged by size: 3290 2702 2034 711 BsaBI GATnn'nnATC Cuts at: 3765 4536 4736 8731 3765 Size: 771 200 3995 3771 Fragments arranged by size: 3995 3771 771 200 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4047 5127 5286 5996 Size: 53 83 186 936 2514 1080 159 710 Cuts at: 5996 6544 6926 7561 275 Size: 548 382 635 1451 Fragments arranged by size: 2514 1451 1080 936 710 635 548 382 186 159 83 53 BsaWI w'CCGG_w Cuts at: 5180 5522 7170 7568 7842 7989 8400 5180 Size: 342 1648 398 274 147 411 5517 Fragments arranged by size: 5517 1648 411 398 342 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2743 2773 3888 3918 5175 Size: 30 165 30 1340 30 1115 30 1257 Cuts at: 5175 5205 5463 5493 5759 5789 1178 Size: 30 258 30 266 30 4126 Fragments arranged by size: 4126 1340 1257 1115 266 258 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2357 2360 2549 3909 4511 4556 4841 4931 Size: 1171 3 189 1360 602 45 285 90 Cuts at: 4931 5697 5767 1186 Size: 766 70 4156 Fragments arranged by size: 4156 1360 1171 766 602 285 189 90 70 45 3 BseYI C'CCAG_C Cuts at: 2333 2589 5743 7940 8436 2333 Size: 256 3154 2197 496 2634 Fragments arranged by size: 3154 2634 2197 496 256 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2172 5576 1163 Size: 573 436 3404 4324 Fragments arranged by size: 4324 3404 573 436 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2566 3586 4403 4875 6003 6187 Size: 275 186 565 1020 817 472 1128 184 Cuts at: 6187 6948 7976 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1128 1028 1020 817 761 565 472 275 186 184 BsiHKAI G_wGCw'C Cuts at: 2259 2880 3723 4086 5826 5961 6798 6883 7954 Size: 621 843 363 1740 135 837 85 1071 Cuts at: 7954 2259 Size: 3042 Fragments arranged by size: 3042 1740 1071 843 837 621 363 135 85 BsmI GAATG_Cn' Cuts at: 6248 6341 6248 Size: 93 8644 BsmBI CGTCTCn'nnnn_ Cuts at: 4747 5028 4747 Size: 281 8456 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2259 2334 2370 2412 2880 3723 Size: 399 232 237 75 36 42 468 843 Cuts at: 3723 4086 4852 5080 5406 5826 5961 6056 6798 Size: 363 766 228 326 420 135 95 742 Cuts at: 6798 6883 7954 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 843 766 742 468 420 399 363 326 237 232 228 135 95 85 75 42 36 BspEI T'CCGG_A Cuts at: 5180 8400 5180 Size: 3220 5517 BspHI T'CATG_A Cuts at: 2854 3979 5004 5151 6626 8356 2854 Size: 1125 1025 147 1475 1730 3235 Fragments arranged by size: 3235 1730 1475 1125 1025 147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2427 3175 5364 6092 844 Size: 1583 748 2189 728 3489 Fragments arranged by size: 3489 2189 1583 748 728 BsrBI CCG'CTC Cuts at: 1394 1823 2033 3045 6624 1394 Size: 429 210 1012 3579 3507 Fragments arranged by size: 3579 3507 1012 429 210 BsrDI GCAATG_nn' Cuts at: 65 1248 7232 7406 65 Size: 1183 5984 174 1396 Fragments arranged by size: 5984 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5488 7381 7568 1700 Size: 110 3678 1893 187 2869 Fragments arranged by size: 3678 2869 1893 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8737 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8737 BssSI C'ACGA_G Cuts at: 2975 5477 5594 6797 7809 2975 Size: 2502 117 1203 1012 3903 Fragments arranged by size: 3903 2502 1203 1012 117 BstAPI GCAn_nnn'nTGC Cuts at: 794 4803 794 Size: 4009 4728 BstBI TT'CG_AA Cuts at: 7541 7541 Size: 8737 BstEII G'GTnAC_C Cuts at: 1184 5708 1184 Size: 4524 4213 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2473 3463 5731 2233 Size: 240 990 2268 5239 Fragments arranged by size: 5239 2268 990 240 BstYI r'GATC_y Cuts at: 1972 3086 3348 5087 5292 5863 6018 6067 6434 Size: 1114 262 1739 205 571 155 49 367 Cuts at: 6434 6819 6836 8288 8374 8386 8732 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1739 1452 1114 571 385 367 346 262 205 155 86 49 17 12 Bsu36I CC'TnA_GG Cuts at: 5311 5646 5311 Size: 335 8402 BtgI C'CryG_G Cuts at: 513 1065 2004 3411 3456 3504 4125 4680 5440 Size: 552 939 1407 45 48 621 555 760 Cuts at: 5440 5583 513 Size: 143 3667 Fragments arranged by size: 3667 1407 939 760 621 555 552 143 48 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3055 3441 5356 487 Size: 683 56 1829 386 1915 3868 Fragments arranged by size: 3868 1915 1829 683 386 56 BtsI GCAGTG_nn' Cuts at: 3362 3841 6253 7047 7067 3362 Size: 479 2412 794 20 5032 Fragments arranged by size: 5032 2412 794 479 20 ClaI AT'CG_AT Cuts at: 1554 5750 1554 Size: 4196 4541 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2898 2933 529 Size: 35 2334 35 6333 Fragments arranged by size: 6333 2334 35 35 DraI TTT'AAA Cuts at: 2871 3558 6041 2871 Size: 687 2483 5567 Fragments arranged by size: 5567 2483 687 DraIII CAC_nnn'GTG Cuts at: 5169 5169 Size: 8737 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7744 809 Size: 507 6428 1802 Fragments arranged by size: 6428 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2604 3384 4122 Size: 54 1425 50 275 18 774 780 738 Cuts at: 4122 4219 4476 4593 4872 5274 5580 6184 7073 Size: 97 257 117 279 402 306 604 889 Cuts at: 7073 8702 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 780 774 738 604 402 306 279 275 257 117 97 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4872 6184 1537 Size: 275 3060 1312 4090 Fragments arranged by size: 4090 3060 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2070 2076 2772 3029 3705 4488 5521 5982 Size: 717 6 696 257 676 783 1033 461 Cuts at: 5982 6667 1353 Size: 685 3423 Fragments arranged by size: 3423 1033 783 717 696 685 676 461 257 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2137 5315 7324 7698 7844 387 Size: 1064 138 548 3178 2009 374 146 1280 Fragments arranged by size: 3178 2009 1280 1064 548 374 146 138 EcoICRI GAG'CTC Cuts at: 3721 3721 Size: 8737 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2235 2428 2981 3032 3341 3635 Size: 393 558 45 193 553 51 309 294 Cuts at: 3635 3683 3686 4037 4955 5255 5794 5879 6800 Size: 48 3 351 918 300 539 85 921 Cuts at: 6800 7397 8184 8452 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 921 918 787 597 558 553 539 393 351 309 300 294 268 193 85 51 48 45 3 EcoNI CCTnn'n_nnAGG Cuts at: 2157 3052 3650 4493 2157 Size: 895 598 843 6401 Fragments arranged by size: 6401 895 843 598 EcoO109I rG'GnC_Cy Cuts at: 2140 2817 5787 2140 Size: 677 2970 5090 Fragments arranged by size: 5090 2970 677 EcoRI G'AATT_C Cuts at: 4359 6046 6424 4359 Size: 1687 378 6672 Fragments arranged by size: 6672 1687 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4985 4985 Size: 8737 FspI TGC'GCA Cuts at: 2828 7243 2828 Size: 4415 4322 HaeII r_GCGC'y Cuts at: 1678 2340 5924 5999 7884 1678 Size: 662 3584 75 1885 2531 Fragments arranged by size: 3584 2531 1885 662 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2103 2135 2611 2643 3049 Size: 32 135 32 531 32 476 32 406 Cuts at: 3049 3071 3081 3103 3158 3190 4280 4312 5175 Size: 22 10 22 55 32 1090 32 863 Cuts at: 5175 5207 5520 5552 5759 5791 5813 5845 5855 Size: 32 313 32 207 32 22 32 10 Cuts at: 5855 5887 7378 7410 7452 7484 1373 Size: 32 1491 32 42 32 2626 Fragments arranged by size: 2626 1491 1090 863 531 476 406 313 207 135 55 42 32 32 32 32 32 32 32 32 32 32 32 32 32 22 22 22 10 10 HincII GTy'rAC Cuts at: 669 1657 1938 2272 3127 3220 4462 5050 6081 Size: 988 281 334 855 93 1242 588 1031 Cuts at: 6081 6327 669 Size: 246 3079 Fragments arranged by size: 3079 1242 1031 988 855 588 334 281 246 93 HindIII A'AGCT_T Cuts at: 748 3824 748 Size: 3076 5661 HpaI GTT'AAC Cuts at: 6327 6327 Size: 8737 KasI G'GCGC_C Cuts at: 5995 5995 Size: 8737 KpnI G_GTAC'C Cuts at: 6062 6062 Size: 8737 MfeI C'AATT_G Cuts at: 6336 6336 Size: 8737 MluI A'CGCG_T Cuts at: 8613 8613 Size: 8737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2439 2567 4552 7851 8035 1305 Size: 1134 128 1985 3299 184 2007 Fragments arranged by size: 3299 2007 1985 1134 184 128 MscI TGG'CCA Cuts at: 10 64 1489 1832 3386 4221 4478 4595 5582 Size: 54 1425 343 1554 835 257 117 987 Cuts at: 5582 10 Size: 3165 Fragments arranged by size: 3165 1554 1425 987 835 343 257 117 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 2327 3503 4583 5003 5204 5276 Size: 648 1065 96 1176 1080 420 201 72 Cuts at: 5276 6695 7054 7213 518 Size: 1419 359 159 2042 Fragments arranged by size: 2042 1419 1176 1080 1065 648 420 359 201 159 96 72 MspA1I CmG'CkG Cuts at: 1606 2589 3280 4234 4418 5233 5410 6153 6830 Size: 983 691 954 184 815 177 743 677 Cuts at: 6830 7978 8223 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 983 954 815 743 691 677 245 184 177 NarI GG'CG_CC Cuts at: 5996 5996 Size: 8737 NcoI C'CATG_G Cuts at: 513 1065 2004 3456 513 Size: 552 939 1452 5794 Fragments arranged by size: 5794 1452 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8737 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8737 NotI GC'GGCC_GC Cuts at: 6184 6184 Size: 8737 NsiI A_TGCA'T Cuts at: 3472 3472 Size: 8737 NspI r_CATG'y Cuts at: 1132 1229 3528 3661 4043 4582 4810 5395 6095 Size: 97 2299 133 382 539 228 585 700 Cuts at: 6095 7640 1132 Size: 1545 2229 Fragments arranged by size: 2299 2229 1545 700 585 539 382 228 133 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8737 PciI A'CATG_T Cuts at: 3524 4039 7636 3524 Size: 515 3597 4625 Fragments arranged by size: 4625 3597 515 PflMI CCAn_nnn'nTGG Cuts at: 1108 4723 1108 Size: 3615 5122 PfoI T'CCnGG_A Cuts at: 2304 3651 2304 Size: 1347 7390 PmeI GTTT'AAAC Cuts at: 6041 6041 Size: 8737 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6806 6838 8319 8351 6806 Size: 32 1481 32 7192 Fragments arranged by size: 7192 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2817 5787 2817 Size: 2970 5767 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8737 PsiI TTA'TAA Cuts at: 4632 4938 6307 4632 Size: 306 1369 7062 Fragments arranged by size: 7062 1369 306 PstI C_TGCA'G Cuts at: 830 2041 3357 4358 5361 6089 830 Size: 1211 1316 1001 1003 728 3478 Fragments arranged by size: 3478 1316 1211 1003 1001 728 PvuI CG_AT'CG Cuts at: 2001 3586 2001 Size: 1585 7152 PvuII CAG'CTG Cuts at: 4234 4418 5233 4234 Size: 184 815 7738 Fragments arranged by size: 7738 815 184 SacI G_AGCT'C Cuts at: 3723 3723 Size: 8737 SalI G'TCGA_C Cuts at: 1936 3218 6079 1936 Size: 1282 2861 4594 Fragments arranged by size: 4594 2861 1282 SanDI GG'GwC_CC Cuts at: 5787 5787 Size: 8737 SapI GCTCTTCn'nnn_ Cuts at: 2772 5982 2772 Size: 3210 5527 SbfI CC_TGCA'GG Cuts at: 5361 6089 5361 Size: 728 8009 ScaI AGT'ACT Cuts at: 6985 8697 6985 Size: 1712 7025 SfcI C'TryA_G Cuts at: 826 1045 2037 3353 3598 4354 5220 5357 6085 Size: 219 992 1316 245 756 866 137 728 Cuts at: 6085 7220 7901 8092 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1316 1135 992 866 756 728 681 245 219 191 137 SfoI GGC'GCC Cuts at: 5997 5997 Size: 8737 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8737 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8737 SmaI CCC'GGG Cuts at: 1872 4378 6064 1872 Size: 2506 1686 4545 Fragments arranged by size: 4545 2506 1686 SmlI C'TyrA_G Cuts at: 820 1017 1943 3095 3248 3515 5348 6841 7742 Size: 197 926 1152 153 267 1833 1493 901 Cuts at: 7742 8004 820 Size: 262 1553 Fragments arranged by size: 1833 1553 1493 1152 926 901 267 262 197 153 SnaBI TAC'GTA Cuts at: 493 3238 6528 493 Size: 2745 3290 2702 Fragments arranged by size: 3290 2745 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8737 SphI G_CATG'C Cuts at: 1132 4810 6095 1132 Size: 3678 1285 3774 Fragments arranged by size: 3774 3678 1285 SspI AAT'ATT Cuts at: 5 52 6661 5 Size: 47 6609 2081 Fragments arranged by size: 6609 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8737 StyI C'CwwG_G Cuts at: 513 1065 2004 2657 2736 3000 3456 5907 6176 Size: 552 939 653 79 264 456 2451 269 Cuts at: 6176 513 Size: 3074 Fragments arranged by size: 3074 2451 939 653 552 456 269 264 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2206 2552 4417 4730 6764 6781 6934 Size: 1255 626 346 1865 313 2034 17 153 Cuts at: 6934 7119 7564 325 Size: 185 445 1498 Fragments arranged by size: 2034 1865 1498 1255 626 445 346 313 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6983 8695 96 Size: 274 80 33 51 1443 5006 1712 138 Fragments arranged by size: 5006 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2743 2775 3624 3656 4677 4709 5463 5495 8697 Size: 32 849 32 1021 32 754 32 3202 Cuts at: 8697 8729 2743 Size: 32 2751 Fragments arranged by size: 3202 2751 1021 849 754 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3061 1316 Size: 1745 6992 XbaI T'CTAG_A Cuts at: 5818 6073 5818 Size: 255 8482 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3007 5088 5623 5920 3007 Size: 2081 535 297 5824 Fragments arranged by size: 5824 2081 535 297 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8737 XmaI C'CCGG_G Cuts at: 1870 4376 6062 1870 Size: 2506 1686 4545 Fragments arranged by size: 4545 2506 1686 XmnI GAAnn'nnTTC Cuts at: 3113 3491 4927 6866 3113 Size: 378 1436 1939 4984 Fragments arranged by size: 4984 1939 1436 378 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6545 276 Size: 53 83 186 936 5011 2468 Fragments arranged by size: 5011 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI