(Circular) (Six-base) MAPSORT of: pFN21AA0256.seq Check: 3620 from: 1 to: 7873 pFN21AA0256 7873 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2912 2912 Size: 7873 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5683 278 Size: 53 83 186 936 4147 2468 Fragments arranged by size: 4147 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2321 5216 1937 Size: 384 2895 4594 Fragments arranged by size: 4594 2895 384 Acc65I G'GTAC_C Cuts at: 4387 5194 4387 Size: 807 7066 AclI AA'CG_TT Cuts at: 1563 6000 6373 1563 Size: 4437 373 3063 Fragments arranged by size: 4437 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4491 4506 4946 5936 7320 7588 1632 Size: 2859 15 440 990 1384 268 1917 Fragments arranged by size: 2859 1917 1384 990 440 268 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7676 AflIII A'CryG_T Cuts at: 1203 3351 6772 7749 1203 Size: 2148 3421 977 1327 Fragments arranged by size: 3421 2148 1327 977 AgeI A'CCGG_T Cuts at: 6704 6704 Size: 7873 AhdI GACnn_n'nnGTC Cuts at: 6602 6602 Size: 7873 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7873 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7873 AlwNI CAG_nnn'CTG Cuts at: 2618 4751 5118 5616 7188 2618 Size: 2133 367 498 1572 3303 Fragments arranged by size: 3303 2133 1572 498 367 ApaLI G'TGCA_C Cuts at: 5930 7086 5930 Size: 1156 6717 ApoI r'AATT_y Cuts at: 1493 4170 4786 5182 5408 5560 7669 1493 Size: 2677 616 396 226 152 2109 1697 Fragments arranged by size: 2677 2109 1697 616 396 226 152 AseI AT'TA_AT Cuts at: 160 1032 6427 160 Size: 872 5395 1606 Fragments arranged by size: 5395 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7873 AvaI C'yCGr_G Cuts at: 1870 1943 5198 1870 Size: 73 3255 4545 Fragments arranged by size: 4545 3255 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4803 4836 4803 Size: 33 7840 BamHI G'GATC_C Cuts at: 3662 5203 5570 3662 Size: 1541 367 5965 Fragments arranged by size: 5965 1541 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4387 5032 5194 6649 618 Size: 325 217 627 2600 645 162 1455 1842 Fragments arranged by size: 2600 1842 1455 645 627 325 217 162 BanII G_rGCy'C Cuts at: 1391 2017 5192 1391 Size: 626 3175 4072 Fragments arranged by size: 4072 3175 626 BbeI G_GCGC'C Cuts at: 5036 5036 Size: 7873 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2467 5001 928 Size: 971 568 2534 3800 Fragments arranged by size: 3800 2534 971 568 BbvCI CC'TCA_GC Cuts at: 2046 4359 4660 2046 Size: 2313 301 5259 Fragments arranged by size: 5259 2313 301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6064 6098 1903 Size: 34 4127 34 3678 Fragments arranged by size: 4127 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4570 5765 6975 4570 Size: 1195 1210 5468 Fragments arranged by size: 5468 1210 1195 BclI T'GATC_A Cuts at: 1548 1548 Size: 7873 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6484 136 Size: 107 122 71 631 605 4812 1525 Fragments arranged by size: 4812 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4777 7868 4777 Size: 3091 4782 BlpI GC'TnA_GC Cuts at: 2298 5290 2298 Size: 2992 4881 Bme1580I G_kGCm'C Cuts at: 1790 2356 5934 7090 1790 Size: 566 3578 1156 2573 Fragments arranged by size: 3578 2573 1156 566 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7873 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3747 4584 5604 Size: 416 53 83 399 24 2330 837 1020 Cuts at: 5604 6562 442 Size: 958 1753 Fragments arranged by size: 2330 1753 1020 958 837 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7873 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4771 4771 Size: 7873 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3756 5066 6533 1239 Size: 2517 1310 1467 2579 Fragments arranged by size: 2579 2517 1467 1310 Bpu10I CC'TnA_GC Cuts at: 2046 3081 4359 4660 2046 Size: 1035 1278 301 5259 Fragments arranged by size: 5259 1278 1035 301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3231 5998 6863 7161 3231 Size: 2767 865 298 3943 Fragments arranged by size: 3943 2767 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2699 4931 4936 6536 882 Size: 617 1200 2232 5 1600 2219 Fragments arranged by size: 2232 2219 1600 1200 617 5 BsaAI yAC'GTr Cuts at: 493 1204 5664 493 Size: 711 4460 2702 Fragments arranged by size: 4460 2702 711 BsaBI GATnn'nnATC Cuts at: 7867 7867 Size: 7873 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5033 5680 6062 6697 Size: 53 83 186 936 3500 647 382 635 Cuts at: 6697 275 Size: 1451 Fragments arranged by size: 3500 1451 936 647 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6306 6704 6978 7125 7536 6306 Size: 398 274 147 411 6643 Fragments arranged by size: 6643 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7648 Fragments arranged by size: 7648 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4319 4871 4874 4877 1186 Size: 3133 552 3 3 4182 Fragments arranged by size: 4182 3133 552 3 3 BseYI C'CCAG_C Cuts at: 2156 4673 7076 7572 2156 Size: 2517 2403 496 2457 Fragments arranged by size: 2517 2457 2403 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2207 2940 3353 4922 1163 Size: 573 471 733 413 1569 4114 Fragments arranged by size: 4114 1569 733 573 471 413 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5323 6084 7112 1540 Size: 275 186 3322 761 1028 2301 Fragments arranged by size: 3322 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5142 5934 6019 7090 5142 Size: 792 85 1071 5925 Fragments arranged by size: 5925 1071 792 85 BsmI GAATG_Cn' Cuts at: 2447 5384 5477 2447 Size: 2937 93 4843 Fragments arranged by size: 4843 2937 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4009 4009 Size: 7873 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2356 5142 5192 5934 6019 7090 Size: 399 227 339 2786 50 742 85 1071 Cuts at: 7090 1391 Size: 2174 Fragments arranged by size: 2786 2174 1071 742 399 339 227 85 50 BspEI T'CCGG_A Cuts at: 7536 7536 Size: 7873 BspHI T'CATG_A Cuts at: 4879 5762 7492 4879 Size: 883 1730 5260 Fragments arranged by size: 5260 1730 883 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2603 2912 5228 844 Size: 1759 309 2316 3489 Fragments arranged by size: 3489 2316 1759 309 BsrBI CCG'CTC Cuts at: 1394 1823 5760 1394 Size: 429 3937 3507 Fragments arranged by size: 3937 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3390 3525 4347 6368 6542 65 Size: 1183 2142 135 822 2021 174 1396 Fragments arranged by size: 2142 2021 1396 1183 822 174 135 BsrFI r'CCGG_y Cuts at: 1700 1810 6517 6704 1700 Size: 110 4707 187 2869 Fragments arranged by size: 4707 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7873 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7873 BssSI C'ACGA_G Cuts at: 5933 6945 5933 Size: 1012 6861 BstAPI GCAn_nnn'nTGC Cuts at: 794 2351 3236 4430 5075 794 Size: 1557 885 1194 645 3592 Fragments arranged by size: 3592 1557 1194 885 645 BstBI TT'CG_AA Cuts at: 6677 6677 Size: 7873 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7873 BstYI r'GATC_y Cuts at: 1972 3662 3991 4777 5203 5570 5955 5972 7424 Size: 1690 329 786 426 367 385 17 1452 Cuts at: 7424 7510 7522 7868 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1690 1452 786 426 385 367 346 329 86 17 12 BstZ17I GTA'TAC Cuts at: 2322 2322 Size: 7873 Bsu36I CC'TnA_GG Cuts at: 5037 5037 Size: 7873 BtgI C'CryG_G Cuts at: 513 1065 2004 2019 4152 513 Size: 552 939 15 2133 4234 Fragments arranged by size: 4234 2133 939 552 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7134 Fragments arranged by size: 7134 683 56 BtsI GCAGTG_nn' Cuts at: 2709 4453 5389 6183 6203 2709 Size: 1744 936 794 20 4379 Fragments arranged by size: 4379 1744 936 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7873 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7838 DraI TTT'AAA Cuts at: 3609 5177 3609 Size: 1568 6305 DraIII CAC_nnn'GTG Cuts at: 2351 2351 Size: 7873 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3808 6880 809 Size: 507 2492 3072 1802 Fragments arranged by size: 3072 2492 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5320 6209 7838 Size: 54 1425 50 275 18 3490 889 1629 Cuts at: 7838 8 Size: 43 Fragments arranged by size: 3490 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5320 1537 Size: 275 3508 4090 Fragments arranged by size: 4090 3508 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2069 2743 3948 5013 5803 1353 Size: 716 674 1205 1065 790 3423 Fragments arranged by size: 3423 1205 1065 790 716 674 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6460 6834 6980 387 Size: 1064 138 4871 374 146 1280 Fragments arranged by size: 4871 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3756 4491 4506 4946 5066 5936 6533 Size: 393 2124 735 15 440 120 870 597 Cuts at: 6533 7320 7588 1239 Size: 787 268 1524 Fragments arranged by size: 2124 1524 870 787 735 597 440 393 268 120 15 EcoNI CCTnn'n_nnAGG Cuts at: 2859 2859 Size: 7873 EcoO109I rG'GnC_Cy Cuts at: 2615 2615 Size: 7873 EcoRI G'AATT_C Cuts at: 4786 5182 5560 4786 Size: 396 378 7099 Fragments arranged by size: 7099 396 378 EcoRV GAT'ATC Cuts at: 2683 2683 Size: 7873 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3652 3652 Size: 7873 FspI TGC'GCA Cuts at: 6379 6379 Size: 7873 HaeII r_GCGC'y Cuts at: 1678 3637 5036 7020 1678 Size: 1959 1399 1984 2531 Fragments arranged by size: 2531 1984 1959 1399 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2508 2540 2612 2644 2660 Size: 32 135 32 936 32 72 32 16 Cuts at: 2660 2692 2881 2913 6514 6546 6588 6620 1373 Size: 32 189 32 3601 32 42 32 2626 Fragments arranged by size: 3601 2626 936 189 135 72 42 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 5217 5463 669 Size: 988 281 3279 246 3079 Fragments arranged by size: 3279 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3219 3291 748 Size: 2471 72 5330 Fragments arranged by size: 5330 2471 72 HpaI GTT'AAC Cuts at: 5463 5463 Size: 7873 KasI G'GCGC_C Cuts at: 5032 5032 Size: 7873 KpnI G_GTAC'C Cuts at: 4391 5198 4391 Size: 807 7066 MfeI C'AATT_G Cuts at: 4617 5472 4617 Size: 855 7018 MluI A'CGCG_T Cuts at: 7749 7749 Size: 7873 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 6987 7171 1305 Size: 1116 4566 184 2007 Fragments arranged by size: 4566 2007 1116 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6051 Fragments arranged by size: 6051 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2914 5831 6190 6349 518 Size: 648 1748 2917 359 159 2042 Fragments arranged by size: 2917 2042 1748 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2045 2335 2572 3308 5289 5966 7114 7359 Size: 439 290 237 736 1981 677 1148 245 Cuts at: 7359 1606 Size: 2120 Fragments arranged by size: 2120 1981 1148 736 677 439 290 245 237 NarI GG'CG_CC Cuts at: 5033 5033 Size: 7873 NcoI C'CATG_G Cuts at: 513 1065 2004 4152 513 Size: 552 939 2148 4234 Fragments arranged by size: 4234 2148 939 552 NdeI CA'TA_TG Cuts at: 387 4053 387 Size: 3666 4207 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7873 NotI GC'GGCC_GC Cuts at: 5320 5320 Size: 7873 NspI r_CATG'y Cuts at: 1132 1229 3355 3751 4831 5231 6776 1132 Size: 97 2126 396 1080 400 1545 2229 Fragments arranged by size: 2229 2126 1545 1080 400 396 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7873 PciI A'CATG_T Cuts at: 3351 6772 3351 Size: 3421 4452 PflMI CCAn_nnn'nTGG Cuts at: 1108 2362 1108 Size: 1254 6619 PfoI T'CCnGG_A Cuts at: 3969 3969 Size: 7873 PmeI GTTT'AAAC Cuts at: 5177 5177 Size: 7873 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5942 5974 7455 7487 5942 Size: 32 1481 32 6328 Fragments arranged by size: 6328 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2615 2615 Size: 7873 PshAI GACnn'nnGTC Cuts at: 1613 3418 1613 Size: 1805 6068 PsiI TTA'TAA Cuts at: 4082 5443 4082 Size: 1361 6512 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3776 3808 3776 Size: 32 7841 PstI C_TGCA'G Cuts at: 830 2831 2860 3442 4426 4448 5123 5225 830 Size: 2001 29 582 984 22 675 102 3478 Fragments arranged by size: 3478 2001 984 675 582 102 29 22 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7873 PvuII CAG'CTG Cuts at: 2045 2335 3308 2045 Size: 290 973 6610 Fragments arranged by size: 6610 973 290 SalI G'TCGA_C Cuts at: 1936 5215 1936 Size: 3279 4594 SapI GCTCTTCn'nnn_ Cuts at: 2743 2743 Size: 7873 SbfI CC_TGCA'GG Cuts at: 5225 5225 Size: 7873 ScaI AGT'ACT Cuts at: 4654 6121 7833 4654 Size: 1467 1712 4694 Fragments arranged by size: 4694 1712 1467 SfcI C'TryA_G Cuts at: 826 1045 2313 2827 2856 3438 4422 4444 4644 Size: 219 1268 514 29 582 984 22 200 Cuts at: 4644 4893 5119 5221 6356 7037 7228 826 Size: 249 226 102 1135 681 191 1471 Fragments arranged by size: 1471 1268 1135 984 681 582 514 249 226 219 200 191 102 29 22 SfoI GGC'GCC Cuts at: 5034 5034 Size: 7873 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7873 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7873 SmaI CCC'GGG Cuts at: 1872 5200 1872 Size: 3328 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3246 5977 6878 7140 820 Size: 197 926 1303 2731 901 262 1553 Fragments arranged by size: 2731 1553 1303 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5664 493 Size: 5171 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7873 SphI G_CATG'C Cuts at: 1132 3751 4831 5231 1132 Size: 2619 1080 400 3774 Fragments arranged by size: 3774 2619 1080 400 SspI AAT'ATT Cuts at: 5 52 3094 5797 5 Size: 47 3042 2703 2081 Fragments arranged by size: 3042 2703 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7873 StyI C'CwwG_G Cuts at: 513 1065 2004 2733 2972 4152 4189 5312 513 Size: 552 939 729 239 1180 37 1123 3074 Fragments arranged by size: 3074 1180 1123 939 729 552 239 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2025 5900 5917 6070 6255 6700 325 Size: 1255 445 3875 17 153 185 445 1498 Fragments arranged by size: 3875 1498 1255 445 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4652 4742 6119 Size: 274 80 33 51 1443 2675 90 1377 Cuts at: 6119 7831 96 Size: 1712 138 Fragments arranged by size: 2675 1712 1443 1377 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2437 2469 3172 3204 4200 4232 5047 5079 7833 Size: 32 703 32 996 32 815 32 2754 Cuts at: 7833 7865 2437 Size: 32 2445 Fragments arranged by size: 2754 2445 996 815 703 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7873 XbaI T'CTAG_A Cuts at: 2999 5209 2999 Size: 2210 5663 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2899 3734 4115 2899 Size: 835 381 6657 Fragments arranged by size: 6657 835 381 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7873 XmaI C'CCGG_G Cuts at: 1870 5198 1870 Size: 3328 4545 XmnI GAAnn'nnTTC Cuts at: 2128 3031 4959 6002 2128 Size: 903 1928 1043 3999 Fragments arranged by size: 3999 1928 1043 903 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5681 276 Size: 53 83 186 936 4147 2468 Fragments arranged by size: 4147 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BfrBI BsiWI BstXI EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI