(Circular) (Six-base) MAPSORT of: pFN21AA0051.seq Check: 6607 from: 1 to: 9676 pFN21AA0051 9676 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4352 4635 6562 4352 Size: 283 1927 7466 Fragments arranged by size: 7466 1927 283 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2113 7486 278 Size: 53 83 186 936 577 5373 2468 Fragments arranged by size: 5373 2468 936 577 186 83 53 AccI GT'mk_AC Cuts at: 1937 2232 7019 1937 Size: 295 4787 4594 Fragments arranged by size: 4787 4594 295 Acc65I G'GTAC_C Cuts at: 6486 6997 6486 Size: 511 9165 AclI AA'CG_TT Cuts at: 1563 6172 7803 8176 1563 Size: 4609 1631 373 3063 Fragments arranged by size: 4609 3063 1631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3536 4032 4340 5525 5576 5684 5813 7739 Size: 1904 496 308 1185 51 108 129 1926 Cuts at: 7739 9123 9391 1632 Size: 1384 268 1917 Fragments arranged by size: 1926 1917 1904 1384 1185 496 308 268 129 108 51 AfeI AGC'GCT Cuts at: 4475 4475 Size: 9676 AflII C'TTAA_G Cuts at: 820 1017 5746 820 Size: 197 4729 4750 Fragments arranged by size: 4750 4729 197 AflIII A'CryG_T Cuts at: 1203 5040 8575 9552 1203 Size: 3837 3535 977 1327 Fragments arranged by size: 3837 3535 1327 977 AgeI A'CCGG_T Cuts at: 8507 8507 Size: 9676 AhdI GACnn_n'nnGTC Cuts at: 8405 8405 Size: 9676 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9676 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2606 6300 1184 Size: 1422 3694 4560 Fragments arranged by size: 4560 3694 1422 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4269 4301 4269 Size: 32 9644 AlwNI CAG_nnn'CTG Cuts at: 3055 3455 4447 4720 4927 6445 6868 7419 8991 Size: 400 992 273 207 1518 423 551 1572 Cuts at: 8991 3055 Size: 3740 Fragments arranged by size: 3740 1572 1518 992 551 423 400 273 207 ApaLI G'TGCA_C Cuts at: 7733 8889 7733 Size: 1156 8520 ApoI r'AATT_y Cuts at: 1493 2697 2889 3545 3819 4138 4150 4295 4312 Size: 1204 192 656 274 319 12 145 17 Cuts at: 4312 4616 5786 5837 6821 6985 7211 7363 9472 Size: 304 1170 51 984 164 226 152 2109 Cuts at: 9472 1493 Size: 1697 Fragments arranged by size: 2109 1697 1204 1170 984 656 319 304 274 226 192 164 152 145 51 17 12 AseI AT'TA_AT Cuts at: 160 1032 2676 3189 3639 3894 5757 8230 160 Size: 872 1644 513 450 255 1863 2473 1606 Fragments arranged by size: 2473 1863 1644 1606 872 513 450 255 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9676 AvaI C'yCGr_G Cuts at: 1870 1943 4167 4557 7001 1870 Size: 73 2224 390 2444 4545 Fragments arranged by size: 4545 2444 2224 390 73 AvrII C'CTAG_G Cuts at: 2176 6647 2176 Size: 4471 5205 BamHI G'GATC_C Cuts at: 4591 7006 7373 4591 Size: 2415 367 6894 Fragments arranged by size: 6894 2415 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5186 6004 6486 6997 8452 Size: 325 217 627 3399 818 482 511 1455 Cuts at: 8452 618 Size: 1842 Fragments arranged by size: 3399 1842 1455 818 627 511 482 325 217 BanII G_rGCy'C Cuts at: 1391 2763 2986 3354 5684 6995 1391 Size: 1372 223 368 2330 1311 4072 Fragments arranged by size: 4072 2330 1372 1311 368 223 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2567 3227 5393 6348 928 Size: 971 668 660 2166 955 4256 Fragments arranged by size: 4256 2166 971 955 668 660 BbvCI CC'TCA_GC Cuts at: 4587 4587 Size: 9676 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2696 2722 2730 2756 4148 4182 4448 Size: 34 759 26 8 26 1392 34 266 Cuts at: 4448 4482 7867 7901 1903 Size: 34 3385 34 3678 Fragments arranged by size: 3678 3385 1392 759 266 34 34 34 34 26 26 8 BciVI GTATCCnnnnn_n' Cuts at: 2468 3279 4271 4361 7568 8778 2468 Size: 811 992 90 3207 1210 3366 Fragments arranged by size: 3366 3207 1210 992 811 90 BclI T'GATC_A Cuts at: 1548 3390 4248 6447 1548 Size: 1842 858 2199 4777 Fragments arranged by size: 4777 2199 1842 858 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8287 136 Size: 107 122 71 631 605 6615 1525 Fragments arranged by size: 6615 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2185 5214 9671 2185 Size: 3029 4457 2190 Fragments arranged by size: 4457 3029 2190 BlpI GC'TnA_GC Cuts at: 2799 7093 2799 Size: 4294 5382 Bme1580I G_kGCm'C Cuts at: 1790 3940 5191 6009 7737 8893 1790 Size: 2150 1251 818 1728 1156 2573 Fragments arranged by size: 2573 2150 1728 1251 1156 818 BmgBI CAC'GTC Cuts at: 1318 6115 6284 1318 Size: 4797 169 4710 Fragments arranged by size: 4797 4710 169 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2255 4078 5219 Size: 416 53 83 399 24 838 1823 1141 Cuts at: 5219 5767 7407 8365 442 Size: 548 1640 958 1753 Fragments arranged by size: 1823 1753 1640 1141 958 838 548 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5372 1056 Size: 4316 5360 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 3144 4124 4742 6141 8336 1239 Size: 989 916 980 618 1399 2195 2579 Fragments arranged by size: 2579 2195 1399 989 980 916 618 Bpu10I CC'TnA_GC Cuts at: 2219 3522 4587 5358 2219 Size: 1303 1065 771 6537 Fragments arranged by size: 6537 1303 1065 771 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3728 4862 7801 8666 8964 3728 Size: 1134 2939 865 298 4440 Fragments arranged by size: 4440 2939 1134 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4100 4880 6203 8339 882 Size: 617 2601 780 1323 2136 2219 Fragments arranged by size: 2601 2219 2136 1323 780 617 BsaAI yAC'GTr Cuts at: 493 1204 7467 493 Size: 711 6263 2702 Fragments arranged by size: 6263 2702 711 BsaBI GATnn'nnATC Cuts at: 3288 9670 3288 Size: 6382 3294 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2110 7483 7865 8500 Size: 53 83 186 936 577 5373 382 635 Cuts at: 8500 275 Size: 1451 Fragments arranged by size: 5373 1451 936 635 577 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5956 6192 8109 8507 8781 8928 9339 5956 Size: 236 1917 398 274 147 411 6293 Fragments arranged by size: 6293 1917 411 398 274 236 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3104 3134 3359 3389 4397 Size: 30 165 30 1701 30 225 30 1008 Cuts at: 4397 4427 6882 6912 1178 Size: 30 2455 30 3942 Fragments arranged by size: 3942 2455 1701 1008 225 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2225 2882 3125 4187 4673 5072 6511 Size: 919 120 657 243 1062 486 399 1439 Cuts at: 6511 1186 Size: 4351 Fragments arranged by size: 4351 1439 1062 919 657 486 399 243 120 BseYI C'CCAG_C Cuts at: 2032 3150 3252 3732 8879 9375 2032 Size: 1118 102 480 5147 496 2333 Fragments arranged by size: 5147 2333 1118 496 480 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 9103 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2048 7126 7887 8915 1540 Size: 275 186 47 5078 761 1028 2301 Fragments arranged by size: 5078 2301 1028 761 275 186 47 BsiHKAI G_wGCw'C Cuts at: 3354 5964 7737 7822 8893 3354 Size: 2610 1773 85 1071 4137 Fragments arranged by size: 4137 2610 1773 1071 85 BsmI GAATG_Cn' Cuts at: 2371 2740 4347 5206 6136 7187 7280 2371 Size: 369 1607 859 930 1051 93 4767 Fragments arranged by size: 4767 1607 1051 930 859 369 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2101 6274 2101 Size: 4173 5503 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2763 2986 3354 3940 5191 5684 5964 Size: 399 973 223 368 586 1251 493 280 Cuts at: 5964 6009 6995 7737 7822 8893 1391 Size: 45 986 742 85 1071 2174 Fragments arranged by size: 2174 1251 1071 986 973 742 586 493 399 368 280 223 85 45 BspEI T'CCGG_A Cuts at: 5956 9339 5956 Size: 3383 6293 BspHI T'CATG_A Cuts at: 5374 6900 7565 9295 5374 Size: 1526 665 1730 5755 Fragments arranged by size: 5755 1730 1526 665 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4352 4416 4635 5073 6562 6611 6750 6876 Size: 3508 64 219 438 1489 49 139 126 Cuts at: 6876 7031 844 Size: 155 3489 Fragments arranged by size: 3508 3489 1489 438 219 155 139 126 64 49 BsrBI CCG'CTC Cuts at: 1394 1823 7563 1394 Size: 429 5740 3507 Fragments arranged by size: 5740 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5490 5685 5949 6456 8171 8345 65 Size: 1183 4242 195 264 507 1715 174 1396 Fragments arranged by size: 4242 1715 1396 1183 507 264 195 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8320 8507 1700 Size: 110 6510 187 2869 Fragments arranged by size: 6510 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9676 BssSI C'ACGA_G Cuts at: 7736 8748 7736 Size: 1012 8664 BstAPI GCAn_nnn'nTGC Cuts at: 794 2657 3143 794 Size: 1863 486 7327 Fragments arranged by size: 7327 1863 486 BstBI TT'CG_AA Cuts at: 6807 8480 6807 Size: 1673 8003 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9676 BstXI CCAn_nnnn'nTGG Cuts at: 2206 4960 5008 6826 2206 Size: 2754 48 1818 5056 Fragments arranged by size: 5056 2754 1818 48 BstYI r'GATC_y Cuts at: 1972 2185 4176 4591 5214 6378 7006 7373 7758 Size: 213 1991 415 623 1164 628 367 385 Cuts at: 7758 7775 9227 9313 9325 9671 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1991 1977 1452 1164 628 623 415 385 367 346 213 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3877 3877 Size: 9676 BtgI C'CryG_G Cuts at: 513 1065 2370 4273 5182 5191 6417 513 Size: 552 1305 1903 909 9 1226 3772 Fragments arranged by size: 3772 1903 1305 1226 909 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8937 Fragments arranged by size: 8937 683 56 BtsI GCAGTG_nn' Cuts at: 3369 7192 7986 8006 3369 Size: 3823 794 20 5039 Fragments arranged by size: 5039 3823 794 20 ClaI AT'CG_AT Cuts at: 1554 5584 1554 Size: 4030 5646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9641 DraI TTT'AAA Cuts at: 6048 6393 6760 6980 6048 Size: 345 367 220 8744 Fragments arranged by size: 8744 367 345 220 DraIII CAC_nnn'GTG Cuts at: 3935 3935 Size: 9676 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3084 6085 8683 809 Size: 507 1768 3001 2598 1802 Fragments arranged by size: 3001 2598 1802 1768 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2045 4233 4962 Size: 54 1425 50 275 18 215 2188 729 Cuts at: 4962 5194 7123 8012 9641 8 Size: 232 1929 889 1629 43 Fragments arranged by size: 2188 1929 1629 1425 889 729 275 232 215 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2045 7123 1537 Size: 275 233 5078 4090 Fragments arranged by size: 5078 4090 275 233 EarI CTCTTCn'nnn_ Cuts at: 1353 2150 2751 3068 3758 4680 4892 6363 7606 Size: 797 601 317 690 922 212 1471 1243 Cuts at: 7606 1353 Size: 3423 Fragments arranged by size: 3423 1471 1243 922 797 690 601 317 212 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 1999 8263 8637 8783 387 Size: 1064 138 410 6264 374 146 1280 Fragments arranged by size: 6264 1280 1064 410 374 146 138 EcoICRI GAG'CTC Cuts at: 3352 3352 Size: 9676 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 3144 3536 4032 4124 4340 4742 Size: 393 596 916 392 496 92 216 402 Cuts at: 4742 5525 5576 5684 5813 6141 7739 8336 9123 Size: 783 51 108 129 328 1598 597 787 Cuts at: 9123 9391 1239 Size: 268 1524 Fragments arranged by size: 1598 1524 916 787 783 597 596 496 402 393 392 328 268 216 129 108 92 51 EcoNI CCTnn'n_nnAGG Cuts at: 2977 3104 5999 2977 Size: 127 2895 6654 Fragments arranged by size: 6654 2895 127 EcoO109I rG'GnC_Cy Cuts at: 3682 6739 6865 3682 Size: 3057 126 6493 Fragments arranged by size: 6493 3057 126 EcoRI G'AATT_C Cuts at: 2697 3545 4295 6985 7363 2697 Size: 848 750 2690 378 5010 Fragments arranged by size: 5010 2690 848 750 378 EcoRV GAT'ATC Cuts at: 2419 3565 4747 2419 Size: 1146 1182 7348 Fragments arranged by size: 7348 1182 1146 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2548 3892 6574 6740 2548 Size: 1344 2682 166 5484 Fragments arranged by size: 5484 2682 1344 166 FspI TGC'GCA Cuts at: 8182 8182 Size: 9676 HaeII r_GCGC'y Cuts at: 1678 4477 5369 8823 1678 Size: 2799 892 3454 2531 Fragments arranged by size: 3454 2799 2531 892 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3104 3136 3359 3391 5131 Size: 32 135 32 1532 32 223 32 1740 Cuts at: 5131 5163 5237 5269 5299 5331 5840 5872 6273 Size: 32 74 32 30 32 509 32 401 Cuts at: 6273 6305 6882 6914 8317 8349 8391 8423 1373 Size: 32 577 32 1403 32 42 32 2626 Fragments arranged by size: 2626 1740 1532 1403 577 509 401 223 135 74 42 32 32 32 32 32 32 32 32 32 32 32 32 30 HincII GTy'rAC Cuts at: 669 1657 1938 2026 2530 6934 7020 7266 669 Size: 988 281 88 504 4404 86 246 3079 Fragments arranged by size: 4404 3079 988 504 281 246 88 86 HindIII A'AGCT_T Cuts at: 748 3570 4914 748 Size: 2822 1344 5510 Fragments arranged by size: 5510 2822 1344 HpaI GTT'AAC Cuts at: 7266 7266 Size: 9676 KpnI G_GTAC'C Cuts at: 6490 7001 6490 Size: 511 9165 MfeI C'AATT_G Cuts at: 3437 7275 3437 Size: 3838 5838 MluI A'CGCG_T Cuts at: 5040 9552 5040 Size: 4512 5164 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2550 3792 3867 4890 5070 5913 6778 6809 Size: 1245 1242 75 1023 180 843 865 31 Cuts at: 6809 8790 8974 1305 Size: 1981 184 2007 Fragments arranged by size: 2007 1981 1245 1242 1023 865 843 184 180 75 31 MscI TGG'CCA Cuts at: 10 64 1489 1832 4235 4964 5196 10 Size: 54 1425 343 2403 729 232 4490 Fragments arranged by size: 4490 2403 1425 729 343 232 54 MslI CAynn'nnrTG Cuts at: 518 1166 2588 4422 4512 5943 7634 7993 8152 Size: 648 1422 1834 90 1431 1691 359 159 Cuts at: 8152 518 Size: 2042 Fragments arranged by size: 2042 1834 1691 1431 1422 648 359 159 90 MspA1I CmG'CkG Cuts at: 1606 3256 3800 4225 4720 6481 7092 7769 8917 Size: 1650 544 425 495 1761 611 677 1148 Cuts at: 8917 9162 1606 Size: 245 2120 Fragments arranged by size: 2120 1761 1650 1148 677 611 544 495 425 245 NcoI C'CATG_G Cuts at: 513 1065 2370 513 Size: 552 1305 7819 Fragments arranged by size: 7819 1305 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9676 NheI G'CTAG_C Cuts at: 1052 5368 1052 Size: 4316 5360 NotI GC'GGCC_GC Cuts at: 7123 7123 Size: 9676 NspI r_CATG'y Cuts at: 1132 1229 2175 2440 2660 3533 5419 5708 7034 Size: 97 946 265 220 873 1886 289 1326 Cuts at: 7034 8579 1132 Size: 1545 2229 Fragments arranged by size: 2229 1886 1545 1326 946 873 289 265 220 97 PacI TTA_AT'TAA Cuts at: 3639 3639 Size: 9676 PasI CC'CwG_GG Cuts at: 1348 3001 1348 Size: 1653 8023 PciI A'CATG_T Cuts at: 8575 8575 Size: 9676 PflMI CCAn_nnn'nTGG Cuts at: 1108 4102 4630 4720 1108 Size: 2994 528 90 6064 Fragments arranged by size: 6064 2994 528 90 PfoI T'CCnGG_A Cuts at: 2064 3786 4304 4500 2064 Size: 1722 518 196 7240 Fragments arranged by size: 7240 1722 518 196 PmeI GTTT'AAAC Cuts at: 6980 6980 Size: 9676 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2243 2275 3321 3353 7745 7777 9258 9290 2243 Size: 32 1046 32 4392 32 1481 32 2629 Fragments arranged by size: 4392 2629 1481 1046 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 6739 6865 6739 Size: 126 9550 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9676 PsiI TTA'TAA Cuts at: 7246 7246 Size: 9676 PstI C_TGCA'G Cuts at: 830 2089 2991 3021 3105 3120 3315 3681 3700 Size: 1259 902 30 84 15 195 366 19 Cuts at: 3700 4263 4494 6876 7028 830 Size: 563 231 2382 152 3478 Fragments arranged by size: 3478 2382 1259 902 563 366 231 195 152 84 30 19 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9676 PvuII CAG'CTG Cuts at: 3256 3800 4225 4720 3256 Size: 544 425 495 8212 Fragments arranged by size: 8212 544 495 425 SacI G_AGCT'C Cuts at: 3354 3354 Size: 9676 SalI G'TCGA_C Cuts at: 1936 7018 1936 Size: 5082 4594 SapI GCTCTTCn'nnn_ Cuts at: 2751 2751 Size: 9676 SbfI CC_TGCA'GG Cuts at: 7028 7028 Size: 9676 ScaI AGT'ACT Cuts at: 4496 5855 7924 9636 4496 Size: 1359 2069 1712 4536 Fragments arranged by size: 4536 2069 1712 1359 SexAI A'CCwGG_T Cuts at: 3591 3591 Size: 9676 SfcI C'TryA_G Cuts at: 826 1045 2085 2412 2987 3017 3101 3116 3311 Size: 219 1040 327 575 30 84 15 195 Cuts at: 3311 3677 3686 3696 4259 4490 6872 6878 7024 Size: 366 9 10 563 231 2382 6 146 Cuts at: 7024 8159 8840 9031 826 Size: 1135 681 191 1471 Fragments arranged by size: 2382 1471 1135 1040 681 575 563 366 327 231 219 195 191 146 84 30 15 10 9 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9676 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9676 SmaI CCC'GGG Cuts at: 1872 7003 1872 Size: 5131 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3707 4877 5746 7780 8681 8943 Size: 197 926 1764 1170 869 2034 901 262 Cuts at: 8943 820 Size: 1553 Fragments arranged by size: 2034 1764 1553 1170 926 901 869 262 197 SnaBI TAC'GTA Cuts at: 493 7467 493 Size: 6974 2702 SpeI A'CTAG_T Cuts at: 152 6511 152 Size: 6359 3317 SphI G_CATG'C Cuts at: 1132 2175 5708 7034 1132 Size: 1043 3533 1326 3774 Fragments arranged by size: 3774 3533 1326 1043 SspI AAT'ATT Cuts at: 5 52 3469 7600 5 Size: 47 3417 4131 2081 Fragments arranged by size: 4131 3417 2081 47 StuI AGG'CCT Cuts at: 1517 2493 4245 1517 Size: 976 1752 6948 Fragments arranged by size: 6948 1752 976 StyI C'CwwG_G Cuts at: 513 1065 2176 2370 2763 6647 7115 513 Size: 552 1111 194 393 3884 468 3074 Fragments arranged by size: 3884 3074 1111 552 468 393 194 SwaI ATTT'AAAT Cuts at: 6048 6048 Size: 9676 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3356 3360 3869 4048 5933 7703 7720 Size: 1255 1776 4 509 179 1885 1770 17 Cuts at: 7720 7873 8058 8503 325 Size: 153 185 445 1498 Fragments arranged by size: 1885 1776 1770 1498 1255 509 445 185 179 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4494 5853 7922 Size: 274 80 33 51 1443 2517 1359 2069 Cuts at: 7922 9634 96 Size: 1712 138 Fragments arranged by size: 2517 2069 1712 1443 1359 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4749 4781 9636 9668 4749 Size: 32 4855 32 4757 Fragments arranged by size: 4855 4757 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3321 1316 Size: 2005 7671 XbaI T'CTAG_A Cuts at: 7012 7012 Size: 9676 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 5005 2203 Size: 2802 6874 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9676 XmaI C'CCGG_G Cuts at: 1870 7001 1870 Size: 5131 4545 XmnI GAAnn'nnTTC Cuts at: 2255 7805 2255 Size: 5550 4126 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2111 7484 276 Size: 53 83 186 936 577 5373 2468 Fragments arranged by size: 5373 2468 936 577 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI