(Circular) (Six-base) MAPSORT of: pFN21ASDF0261.seq Check: 6023 from: 1 to: 9520 pFN21ASDF0261 9520 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3004 3619 6285 6706 3004 Size: 615 2666 421 5818 Fragments arranged by size: 5818 2666 615 421 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5706 7330 278 Size: 53 83 186 936 4170 1624 2468 Fragments arranged by size: 4170 2468 1624 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5811 6863 1937 Size: 3874 1052 4594 Fragments arranged by size: 4594 3874 1052 Acc65I G'GTAC_C Cuts at: 6841 6841 Size: 9520 AclI AA'CG_TT Cuts at: 1563 7647 8020 1563 Size: 6084 373 3063 Fragments arranged by size: 6084 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2526 3612 4049 4301 5157 6026 6779 7583 Size: 894 1086 437 252 856 869 753 804 Cuts at: 7583 8967 9235 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1086 894 869 856 804 753 437 268 252 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9323 AflIII A'CryG_T Cuts at: 1203 6263 8419 9396 1203 Size: 5060 2156 977 1327 Fragments arranged by size: 5060 2156 1327 977 AgeI A'CCGG_T Cuts at: 8351 8351 Size: 9520 AhdI GACnn_n'nnGTC Cuts at: 8249 8249 Size: 9520 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9520 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5531 1184 Size: 4347 5173 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5838 5870 5838 Size: 32 9488 AlwNI CAG_nnn'CTG Cuts at: 3686 5140 5222 5365 5863 6757 7263 8835 3686 Size: 1454 82 143 498 894 506 1572 4371 Fragments arranged by size: 4371 1572 1454 894 506 498 143 82 ApaLI G'TGCA_C Cuts at: 6747 7577 8733 6747 Size: 830 1156 7534 Fragments arranged by size: 7534 1156 830 ApoI r'AATT_y Cuts at: 1493 2078 2474 5558 6829 7055 7207 9316 1493 Size: 585 396 3084 1271 226 152 2109 1697 Fragments arranged by size: 3084 2109 1697 1271 585 396 226 152 AseI AT'TA_AT Cuts at: 160 1032 8074 160 Size: 872 7042 1606 Fragments arranged by size: 7042 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9520 AvaI C'yCGr_G Cuts at: 1870 1943 3320 4617 5387 6315 6591 6845 1870 Size: 73 1377 1297 770 928 276 254 4545 Fragments arranged by size: 4545 1377 1297 928 770 276 254 73 AvrII C'CTAG_G Cuts at: 4312 4312 Size: 9520 BamHI G'GATC_C Cuts at: 3482 4182 6850 7217 3482 Size: 700 2668 367 5785 Fragments arranged by size: 5785 2668 700 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3016 3127 3293 3812 6446 Size: 325 217 627 1229 111 166 519 2634 Cuts at: 6446 6841 8296 618 Size: 395 1455 1842 Fragments arranged by size: 2634 1842 1455 1229 627 519 395 325 217 166 111 BanII G_rGCy'C Cuts at: 1391 2286 2394 2685 2901 3387 3425 4857 5171 Size: 895 108 291 216 486 38 1432 314 Cuts at: 5171 6839 1391 Size: 1668 4072 Fragments arranged by size: 4072 1668 1432 895 486 314 291 216 108 38 BbeI G_GCGC'C Cuts at: 6450 6450 Size: 9520 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5541 928 Size: 971 3642 4907 Fragments arranged by size: 4907 3642 971 BbvCI CC'TCA_GC Cuts at: 2031 2307 3345 3491 4941 2031 Size: 276 1038 146 1450 6610 Fragments arranged by size: 6610 1450 1038 276 146 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7711 7745 1903 Size: 34 5774 34 3678 Fragments arranged by size: 5774 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7412 8622 7412 Size: 1210 8310 BclI T'GATC_A Cuts at: 1548 1548 Size: 9520 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2358 3922 5044 Size: 107 122 71 631 605 686 1564 1122 Cuts at: 5044 5136 6050 8131 136 Size: 92 914 2081 1525 Fragments arranged by size: 2081 1564 1525 1122 914 686 631 605 122 107 92 71 BglII A'GATC_T Cuts at: 9515 9515 Size: 9520 BlpI GC'TnA_GC Cuts at: 6937 6937 Size: 9520 Bme1580I G_kGCm'C Cuts at: 1790 3697 6751 7581 8737 1790 Size: 1907 3054 830 1156 2573 Fragments arranged by size: 3054 2573 1907 1156 830 BmgBI CAC'GTC Cuts at: 1318 6628 1318 Size: 5310 4210 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2341 2913 3428 Size: 416 53 83 399 24 924 572 515 Cuts at: 3428 3576 3629 7251 8209 442 Size: 148 53 3622 958 1753 Fragments arranged by size: 3622 1753 958 924 572 515 416 399 148 83 53 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9520 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2554 2979 2554 Size: 425 9095 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2732 3663 3855 4841 5039 5146 5387 5449 Size: 1493 931 192 986 198 107 241 62 Cuts at: 5449 5927 5978 6086 6563 6635 6686 8180 1239 Size: 478 51 108 477 72 51 1494 2579 Fragments arranged by size: 2579 1494 1493 986 931 478 477 241 198 192 108 107 72 62 51 51 Bpu10I CC'TnA_GC Cuts at: 2031 2307 2964 3345 3491 4425 4941 6134 2031 Size: 276 657 381 146 934 516 1193 5417 Fragments arranged by size: 5417 1193 934 657 516 381 276 146 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4026 4504 5924 7645 8510 8808 4026 Size: 478 1420 1721 865 298 4738 Fragments arranged by size: 4738 1721 1420 865 478 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2125 2591 4060 4105 4894 5431 8183 Size: 617 626 466 1469 45 789 537 2752 Cuts at: 8183 882 Size: 2219 Fragments arranged by size: 2752 2219 1469 789 626 617 537 466 45 BsaAI yAC'GTr Cuts at: 493 1204 3674 6817 7311 493 Size: 711 2470 3143 494 2702 Fragments arranged by size: 3143 2702 2470 711 494 BsaBI GATnn'nnATC Cuts at: 9514 9514 Size: 9520 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5027 5703 6447 7327 Size: 53 83 186 936 3494 676 744 880 Cuts at: 7327 7709 8344 275 Size: 382 635 1451 Fragments arranged by size: 3494 1451 936 880 744 676 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2323 4849 7953 8351 8625 8772 9183 2323 Size: 2526 3104 398 274 147 411 2660 Fragments arranged by size: 3104 2660 2526 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2365 2395 3769 3799 4117 Size: 30 165 30 962 30 1374 30 318 Cuts at: 4117 4147 5012 5042 5360 5390 5976 6006 1178 Size: 30 865 30 318 30 586 30 4692 Fragments arranged by size: 4692 1374 962 865 586 318 318 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2046 2048 2122 2480 2675 2729 3217 3230 Size: 860 2 74 358 195 54 488 13 Cuts at: 3230 3261 3452 3543 3567 4194 4231 4290 4618 Size: 31 191 91 24 627 37 59 328 Cuts at: 4618 5287 5419 6016 6047 6134 6349 6542 1186 Size: 669 132 597 31 87 215 193 4164 Fragments arranged by size: 4164 860 669 627 597 488 358 328 215 195 193 191 132 91 87 74 59 54 37 31 31 24 13 2 BseYI C'CCAG_C Cuts at: 2040 3181 3796 4070 4082 5003 5301 8723 9219 Size: 1141 615 274 12 921 298 3422 496 Cuts at: 9219 2040 Size: 2341 Fragments arranged by size: 3422 2341 1141 921 615 496 298 274 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3654 4997 6263 6731 1163 Size: 573 1918 1343 1266 468 3952 Fragments arranged by size: 3952 1918 1343 1266 573 468 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6970 7731 8759 1540 Size: 275 186 4969 761 1028 2301 Fragments arranged by size: 4969 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2394 3398 3679 4616 4857 5667 6751 7581 7666 Size: 1004 281 937 241 810 1084 830 85 Cuts at: 7666 8737 2394 Size: 1071 3177 Fragments arranged by size: 3177 1084 1071 1004 937 830 810 281 241 85 BsmI GAATG_Cn' Cuts at: 7031 7124 7031 Size: 93 9427 BsmBI CGTCTCn'nnnn_ Cuts at: 2234 5694 2234 Size: 3460 6060 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2286 2394 2685 2901 3387 3398 3425 Size: 399 496 108 291 216 486 11 27 Cuts at: 3425 3679 3697 4616 4857 5171 5667 6751 6839 Size: 254 18 919 241 314 496 1084 88 Cuts at: 6839 7581 7666 8737 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1084 1071 919 742 496 496 486 399 314 291 254 241 216 108 88 85 27 18 11 BspEI T'CCGG_A Cuts at: 2323 4849 9183 2323 Size: 2526 4334 2660 Fragments arranged by size: 4334 2660 2526 BspHI T'CATG_A Cuts at: 3306 7409 9139 3306 Size: 4103 1730 3687 Fragments arranged by size: 4103 3687 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2803 3004 3619 3943 4669 5451 5598 5823 Size: 1959 201 615 324 726 782 147 225 Cuts at: 5823 6285 6706 6875 844 Size: 462 421 169 3489 Fragments arranged by size: 3489 1959 782 726 615 462 421 324 225 201 169 147 BsrBI CCG'CTC Cuts at: 1394 1823 3248 6172 7407 1394 Size: 429 1425 2924 1235 3507 Fragments arranged by size: 3507 2924 1425 1235 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4580 8015 8189 65 Size: 1183 3332 3435 174 1396 Fragments arranged by size: 3435 3332 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5130 8164 8351 1700 Size: 110 3320 3034 187 2869 Fragments arranged by size: 3320 3034 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9520 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9520 BssSI C'ACGA_G Cuts at: 4260 7580 8592 4260 Size: 3320 1012 5188 Fragments arranged by size: 5188 3320 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5140 794 Size: 4346 5174 BstBI TT'CG_AA Cuts at: 2007 8324 2007 Size: 6317 3203 BstEII G'GTnAC_C Cuts at: 1184 4968 1184 Size: 3784 5736 BstXI CCAn_nnnn'nTGG Cuts at: 2394 2449 3165 2394 Size: 55 716 8749 Fragments arranged by size: 8749 716 55 BstYI r'GATC_y Cuts at: 1972 2507 3482 4182 4524 5346 6501 6850 7217 Size: 535 975 700 342 822 1155 349 367 Cuts at: 7217 7602 7619 9071 9157 9169 9515 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1155 975 822 700 535 385 367 349 346 342 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2094 2658 3261 4902 5163 2094 Size: 564 603 1641 261 6451 Fragments arranged by size: 6451 1641 603 564 261 BtgI C'CryG_G Cuts at: 513 1065 2022 4167 4236 5193 5402 5457 5493 Size: 552 957 2145 69 957 209 55 36 Cuts at: 5493 513 Size: 4540 Fragments arranged by size: 4540 2145 957 957 552 209 69 55 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8781 Fragments arranged by size: 8781 683 56 BtsI GCAGTG_nn' Cuts at: 3195 5083 6658 7036 7830 7850 3195 Size: 1888 1575 378 794 20 4865 Fragments arranged by size: 4865 1888 1575 794 378 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9520 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4563 4598 529 Size: 35 3999 35 5451 Fragments arranged by size: 5451 3999 35 35 DraI TTT'AAA Cuts at: 6824 6824 Size: 9520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3439 4074 4665 4679 8527 809 Size: 507 2123 635 591 14 3848 1802 Fragments arranged by size: 3848 2123 1802 635 591 507 14 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2480 4252 4374 Size: 54 1425 50 275 18 650 1772 122 Cuts at: 4374 4722 6513 6967 7856 9485 8 Size: 348 1791 454 889 1629 43 Fragments arranged by size: 1791 1772 1629 1425 889 650 454 348 275 122 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6967 1537 Size: 275 5155 4090 Fragments arranged by size: 5155 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2457 2523 2545 2577 2640 2988 2996 3018 Size: 1104 66 22 32 63 348 8 22 Cuts at: 3018 3190 3207 4831 5176 5681 5727 6002 6290 Size: 172 17 1624 345 505 46 275 288 Cuts at: 6290 6470 7450 1353 Size: 180 980 3423 Fragments arranged by size: 3423 1624 1104 980 505 348 345 288 275 180 172 66 63 46 32 22 22 17 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3050 5792 8107 8481 8627 387 Size: 1064 138 1461 2742 2315 374 146 1280 Fragments arranged by size: 2742 2315 1461 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2392 4855 2392 Size: 2463 7057 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2526 2732 3612 3663 3855 4049 4301 Size: 393 894 206 880 51 192 194 252 Cuts at: 4301 4841 5039 5146 5157 5387 5449 5927 5978 Size: 540 198 107 11 230 62 478 51 Cuts at: 5978 6026 6086 6563 6635 6686 6779 7583 8180 Size: 48 60 477 72 51 93 804 597 Cuts at: 8180 8967 9235 1239 Size: 787 268 1524 Fragments arranged by size: 1524 894 880 804 787 597 540 478 477 393 268 252 230 206 198 194 192 107 93 72 62 60 51 51 51 48 11 EcoNI CCTnn'n_nnAGG Cuts at: 3646 4944 5021 5339 5480 3646 Size: 1298 77 318 141 7686 Fragments arranged by size: 7686 1298 318 141 77 EcoO109I rG'GnC_Cy Cuts at: 2155 3630 3792 3924 4862 4906 5137 5210 5419 Size: 1475 162 132 938 44 231 73 209 Cuts at: 5419 2155 Size: 6256 Fragments arranged by size: 6256 1475 938 231 209 162 132 73 44 EcoRI G'AATT_C Cuts at: 2078 6829 7207 2078 Size: 4751 378 4391 Fragments arranged by size: 4751 4391 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4364 4680 4364 Size: 316 9204 FspI TGC'GCA Cuts at: 5144 5501 5594 8026 5144 Size: 357 93 2432 6638 Fragments arranged by size: 6638 2432 357 93 HaeII r_GCGC'y Cuts at: 1678 5712 5932 6450 8667 1678 Size: 4034 220 518 2217 2531 Fragments arranged by size: 4034 2531 2217 518 220 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2144 2176 2365 2397 2404 Size: 32 135 32 572 32 189 32 7 Cuts at: 2404 2436 3338 3370 3701 3733 3769 3801 4924 Size: 32 902 32 331 32 36 32 1123 Cuts at: 4924 4956 5012 5044 5139 5171 5360 5392 6625 Size: 32 56 32 95 32 189 32 1233 Cuts at: 6625 6657 8161 8193 8235 8267 1373 Size: 32 1504 32 42 32 2626 Fragments arranged by size: 2626 1504 1233 1123 902 572 331 189 189 135 95 56 42 36 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 6864 7110 669 Size: 988 281 4926 246 3079 Fragments arranged by size: 4926 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 6491 748 Size: 5743 3777 HpaI GTT'AAC Cuts at: 7110 7110 Size: 9520 KasI G'GCGC_C Cuts at: 6446 6446 Size: 9520 KpnI G_GTAC'C Cuts at: 6845 6845 Size: 9520 MfeI C'AATT_G Cuts at: 7119 7119 Size: 9520 MluI A'CGCG_T Cuts at: 6263 9396 6263 Size: 3133 6387 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5647 5679 5989 6442 6513 6756 8634 8818 Size: 4342 32 310 453 71 243 1878 184 Cuts at: 8818 1305 Size: 2007 Fragments arranged by size: 4342 2007 1878 453 310 243 184 71 32 MscI TGG'CCA Cuts at: 10 64 1489 1832 2482 4254 4376 6515 10 Size: 54 1425 343 650 1772 122 2139 3015 Fragments arranged by size: 3015 2139 1772 1425 650 343 122 54 MslI CAynn'nnrTG Cuts at: 518 1166 4260 5609 7478 7837 7996 518 Size: 648 3094 1349 1869 359 159 2042 Fragments arranged by size: 3094 2042 1869 1349 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3138 4370 5034 5305 5362 5404 5449 5469 Size: 1532 1232 664 271 57 42 45 20 Cuts at: 5469 6073 6796 6936 7613 8761 9006 1606 Size: 604 723 140 677 1148 245 2120 Fragments arranged by size: 2120 1532 1232 1148 723 677 664 604 271 245 140 57 45 42 20 NaeI GCC'GGC Cuts at: 5132 5132 Size: 9520 NarI GG'CG_CC Cuts at: 6447 6447 Size: 9520 NcoI C'CATG_G Cuts at: 513 1065 2022 4167 5457 513 Size: 552 957 2145 1290 4576 Fragments arranged by size: 4576 2145 1290 957 552 NdeI CA'TA_TG Cuts at: 387 4629 387 Size: 4242 5278 NgoMIV G'CCGG_C Cuts at: 5130 5130 Size: 9520 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9520 NotI GC'GGCC_GC Cuts at: 6967 6967 Size: 9520 NspI r_CATG'y Cuts at: 1132 1229 4331 6878 8423 1132 Size: 97 3102 2547 1545 2229 Fragments arranged by size: 3102 2547 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4095 4961 6239 1348 Size: 2747 866 1278 4629 Fragments arranged by size: 4629 2747 1278 866 PciI A'CATG_T Cuts at: 8419 8419 Size: 9520 PflMI CCAn_nnn'nTGG Cuts at: 1108 2710 4842 6064 1108 Size: 1602 2132 1222 4564 Fragments arranged by size: 4564 2132 1602 1222 PfoI T'CCnGG_A Cuts at: 5206 5886 6078 5206 Size: 680 192 8648 Fragments arranged by size: 8648 680 192 PmeI GTTT'AAAC Cuts at: 6824 6824 Size: 9520 PmlI CAC'GTG Cuts at: 3674 3674 Size: 9520 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2172 2204 7589 7621 9102 9134 2172 Size: 32 5385 32 1481 32 2558 Fragments arranged by size: 5385 2558 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2155 4862 5210 2155 Size: 2707 348 6465 Fragments arranged by size: 6465 2707 348 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9520 PsiI TTA'TAA Cuts at: 7090 7090 Size: 9520 PstI C_TGCA'G Cuts at: 830 2275 2596 2670 3015 5448 6872 830 Size: 1445 321 74 345 2433 1424 3478 Fragments arranged by size: 3478 2433 1445 1424 345 321 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9520 PvuII CAG'CTG Cuts at: 5034 5305 6073 6796 5034 Size: 271 768 723 7758 Fragments arranged by size: 7758 768 723 271 SacI G_AGCT'C Cuts at: 2394 4857 2394 Size: 2463 7057 SacII CC_GC'GG Cuts at: 5405 5405 Size: 9520 SalI G'TCGA_C Cuts at: 1936 6862 1936 Size: 4926 4594 SanDI GG'GwC_CC Cuts at: 4862 5210 4862 Size: 348 9172 SapI GCTCTTCn'nnn_ Cuts at: 2577 2640 3190 5176 5681 2577 Size: 63 550 1986 505 6416 Fragments arranged by size: 6416 1986 550 505 63 SbfI CC_TGCA'GG Cuts at: 6872 6872 Size: 9520 ScaI AGT'ACT Cuts at: 7768 9480 7768 Size: 1712 7808 SexAI A'CCwGG_T Cuts at: 3876 3876 Size: 9520 SfcI C'TryA_G Cuts at: 826 1045 2271 2592 2666 3011 3943 3988 4528 Size: 219 1226 321 74 345 932 45 540 Cuts at: 4528 5444 6791 6868 8003 8684 8875 826 Size: 916 1347 77 1135 681 191 1471 Fragments arranged by size: 1471 1347 1226 1135 932 916 681 540 345 321 219 191 77 74 45 SfoI GGC'GCC Cuts at: 6448 6448 Size: 9520 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9520 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9520 SmaI CCC'GGG Cuts at: 1872 3322 5389 6847 1872 Size: 1450 2067 1458 4545 Fragments arranged by size: 4545 2067 1458 1450 SmlI C'TyrA_G Cuts at: 820 1017 1943 4041 4519 5939 7624 8525 8787 Size: 197 926 2098 478 1420 1685 901 262 Cuts at: 8787 820 Size: 1553 Fragments arranged by size: 2098 1685 1553 1420 926 901 478 262 197 SnaBI TAC'GTA Cuts at: 493 6817 7311 493 Size: 6324 494 2702 Fragments arranged by size: 6324 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9520 SphI G_CATG'C Cuts at: 1132 4331 6878 1132 Size: 3199 2547 3774 Fragments arranged by size: 3774 3199 2547 SspI AAT'ATT Cuts at: 5 52 6165 7444 5 Size: 47 6113 1279 2081 Fragments arranged by size: 6113 2081 1279 47 StuI AGG'CCT Cuts at: 1517 2312 4534 5582 1517 Size: 795 2222 1048 5455 Fragments arranged by size: 5455 2222 1048 795 StyI C'CwwG_G Cuts at: 513 1065 2022 2127 2259 3514 3557 3788 4167 Size: 552 957 105 132 1255 43 231 379 Cuts at: 4167 4312 5457 6219 6510 6959 513 Size: 145 1145 762 291 449 3074 Fragments arranged by size: 3074 1255 1145 957 762 552 449 379 291 231 145 132 105 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4241 7547 7564 7717 7902 8347 325 Size: 1255 2661 3306 17 153 185 445 1498 Fragments arranged by size: 3306 2661 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7766 9478 96 Size: 274 80 33 51 1443 5789 1712 138 Fragments arranged by size: 5789 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4117 4149 5073 5105 9480 9512 4117 Size: 32 924 32 4375 32 4125 Fragments arranged by size: 4375 4125 924 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3334 5777 5918 1316 Size: 2018 2443 141 4918 Fragments arranged by size: 4918 2443 2018 141 XbaI T'CTAG_A Cuts at: 2422 4060 6856 2422 Size: 1638 2796 5086 Fragments arranged by size: 5086 2796 1638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2166 3415 6793 2166 Size: 1249 3378 4893 Fragments arranged by size: 4893 3378 1249 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9520 XmaI C'CCGG_G Cuts at: 1870 3320 5387 6845 1870 Size: 1450 2067 1458 4545 Fragments arranged by size: 4545 2067 1458 1450 XmnI GAAnn'nnTTC Cuts at: 6492 7649 6492 Size: 1157 8363 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5704 7328 276 Size: 53 83 186 936 4170 1624 2468 Fragments arranged by size: 4170 2468 1624 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SfiI SrfI SwaI