(Circular) (Six-base) MAPSORT of: pFN21ASDF0187.seq Check: 99 from: 1 to: 6418 pFN21ASDF0187 6418 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4228 278 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3761 1937 Size: 1824 4594 Acc65I G'GTAC_C Cuts at: 3739 3739 Size: 6418 AclI AA'CG_TT Cuts at: 1563 4545 4918 1563 Size: 2982 373 3063 Fragments arranged by size: 3063 2982 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2182 2273 3079 3144 3247 3331 3415 4481 Size: 550 91 806 65 103 84 84 1066 Cuts at: 4481 5865 6133 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1066 806 550 268 103 91 84 84 65 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6221 AflIII A'CryG_T Cuts at: 1203 2130 5317 6294 1203 Size: 927 3187 977 1327 Fragments arranged by size: 3187 1327 977 927 AgeI A'CCGG_T Cuts at: 5249 5249 Size: 6418 AhdI GACnn_n'nnGTC Cuts at: 2447 5147 2447 Size: 2700 3718 AleI CACnn'nnGTG Cuts at: 1166 3156 1166 Size: 1990 4428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6418 AlwNI CAG_nnn'CTG Cuts at: 2275 2308 2758 4161 5733 2275 Size: 33 450 1403 1572 2960 Fragments arranged by size: 2960 1572 1403 450 33 ApaI G_GGCC'C Cuts at: 2232 2232 Size: 6418 ApaLI G'TGCA_C Cuts at: 3577 4475 5631 3577 Size: 898 1156 4364 Fragments arranged by size: 4364 1156 898 ApoI r'AATT_y Cuts at: 1493 3465 3549 3727 3953 4105 6214 1493 Size: 1972 84 178 226 152 2109 1697 Fragments arranged by size: 2109 1972 1697 226 178 152 84 AseI AT'TA_AT Cuts at: 160 1032 4972 160 Size: 872 3940 1606 Fragments arranged by size: 3940 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 AvaI C'yCGr_G Cuts at: 1870 1943 2232 2521 3558 3743 1870 Size: 73 289 289 1037 185 4545 Fragments arranged by size: 4545 1037 289 289 185 73 BamHI G'GATC_C Cuts at: 2503 3297 3748 4115 2503 Size: 794 451 367 4806 Fragments arranged by size: 4806 794 451 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2494 3739 5194 618 Size: 325 217 627 707 1245 1455 1842 Fragments arranged by size: 1842 1455 1245 707 627 325 217 BanII G_rGCy'C Cuts at: 1391 2232 2358 2605 3107 3443 3737 1391 Size: 841 126 247 502 336 294 4072 Fragments arranged by size: 4072 841 502 336 294 247 126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 2424 928 Size: 971 231 294 4922 Fragments arranged by size: 4922 971 294 231 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4609 4643 1903 Size: 34 2672 34 3678 Fragments arranged by size: 3678 2672 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4310 5520 4310 Size: 1210 5208 BclI T'GATC_A Cuts at: 1548 1548 Size: 6418 BfrBI ATG'CAT Cuts at: 3683 3683 Size: 6418 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5029 136 Size: 107 122 71 631 605 3357 1525 Fragments arranged by size: 3357 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6413 6413 Size: 6418 BlpI GC'TnA_GC Cuts at: 3835 3835 Size: 6418 Bme1580I G_kGCm'C Cuts at: 1790 2232 3581 4479 5635 1790 Size: 442 1349 898 1156 2573 Fragments arranged by size: 2573 1349 1156 898 442 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6418 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2545 4149 5107 Size: 416 53 83 399 24 1128 1604 958 Cuts at: 5107 442 Size: 1753 Fragments arranged by size: 1753 1604 1128 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6418 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2258 2872 3670 2258 Size: 614 798 5006 Fragments arranged by size: 5006 798 614 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2154 2330 2342 2756 3243 3411 5078 1239 Size: 915 176 12 414 487 168 1667 2579 Fragments arranged by size: 2579 1667 915 487 414 176 168 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4543 5408 5706 4543 Size: 865 298 5255 Fragments arranged by size: 5255 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2646 5081 882 Size: 617 1147 2435 2219 Fragments arranged by size: 2435 2219 1147 617 BsaAI yAC'GTr Cuts at: 493 1204 3715 4209 493 Size: 711 2511 494 2702 Fragments arranged by size: 2702 2511 711 494 BsaBI GATnn'nnATC Cuts at: 6412 6412 Size: 6418 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4225 4607 5242 275 Size: 53 83 186 936 2692 382 635 1451 Fragments arranged by size: 2692 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4851 5249 5523 5670 6081 4851 Size: 398 274 147 411 5188 Fragments arranged by size: 5188 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2152 2182 1178 Size: 30 165 30 749 30 5414 Fragments arranged by size: 5414 749 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2157 2411 2861 2996 1186 Size: 971 254 450 135 4608 Fragments arranged by size: 4608 971 450 254 135 BseYI C'CCAG_C Cuts at: 3667 5621 6117 3667 Size: 1954 496 3968 Fragments arranged by size: 3968 1954 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2055 2610 2655 1163 Size: 573 319 555 45 4926 Fragments arranged by size: 4926 573 555 319 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3868 4629 5657 1540 Size: 275 186 1867 761 1028 2301 Fragments arranged by size: 2301 1867 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2358 2605 3107 3443 3581 4479 4564 5635 2358 Size: 247 502 336 138 898 85 1071 3141 Fragments arranged by size: 3141 1071 898 502 336 247 138 85 BsmI GAATG_Cn' Cuts at: 3929 4022 3929 Size: 93 6325 BsmBI CGTCTCn'nnnn_ Cuts at: 2577 2577 Size: 6418 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2358 2605 3107 3443 3581 3737 Size: 399 442 126 247 502 336 138 156 Cuts at: 3737 4479 4564 5635 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 502 442 399 336 247 156 138 126 85 BspEI T'CCGG_A Cuts at: 6081 6081 Size: 6418 BspHI T'CATG_A Cuts at: 2635 3319 4307 6037 2635 Size: 684 988 1730 3016 Fragments arranged by size: 3016 1730 988 684 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3773 844 Size: 2929 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4305 1394 Size: 429 2482 3507 Fragments arranged by size: 3507 2482 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2703 4913 5087 65 Size: 1183 1455 2210 174 1396 Fragments arranged by size: 2210 1455 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5062 5249 1700 Size: 110 3252 187 2869 Fragments arranged by size: 3252 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6418 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6418 BssSI C'ACGA_G Cuts at: 2540 2598 3181 4478 5490 2540 Size: 58 583 1297 1012 3468 Fragments arranged by size: 3468 1297 1012 583 58 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6418 BstBI TT'CG_AA Cuts at: 2007 5222 2007 Size: 3215 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6418 BstYI r'GATC_y Cuts at: 1972 2304 2503 2909 3297 3748 4115 4500 4517 Size: 332 199 406 388 451 367 385 17 Cuts at: 4517 5969 6055 6067 6413 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 451 406 388 385 367 346 332 199 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2979 2979 Size: 6418 BtgI C'CryG_G Cuts at: 513 1065 2049 3135 513 Size: 552 984 1086 3796 Fragments arranged by size: 3796 1086 984 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2675 487 Size: 683 56 1449 4230 Fragments arranged by size: 4230 1449 683 56 BtsI GCAGTG_nn' Cuts at: 3934 4728 4748 3934 Size: 794 20 5604 Fragments arranged by size: 5604 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6418 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6383 DraI TTT'AAA Cuts at: 3722 3722 Size: 6418 DraIII CAC_nnn'GTG Cuts at: 3585 3585 Size: 6418 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5425 809 Size: 507 4109 1802 Fragments arranged by size: 4109 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2279 3865 4754 Size: 54 1425 50 275 18 449 1586 889 Cuts at: 4754 6383 8 Size: 1629 43 Fragments arranged by size: 1629 1586 1425 889 449 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3865 1537 Size: 275 2053 4090 Fragments arranged by size: 4090 2053 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2103 2610 4348 1353 Size: 750 507 1738 3423 Fragments arranged by size: 3423 1738 750 507 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2177 3191 5005 5379 5525 387 Size: 1064 138 588 1014 1814 374 146 1280 Fragments arranged by size: 1814 1280 1064 1014 588 374 146 138 EcoICRI GAG'CTC Cuts at: 2356 2603 3105 3441 2356 Size: 247 502 336 5333 Fragments arranged by size: 5333 502 336 247 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2182 2273 2330 2342 2756 3079 Size: 393 522 28 91 57 12 414 323 Cuts at: 3079 3144 3243 3247 3331 3411 3415 4481 5078 Size: 65 99 4 84 80 4 1066 597 Cuts at: 5078 5865 6133 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1066 787 597 522 414 393 323 268 99 91 84 80 65 57 28 12 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 2348 2348 Size: 6418 EcoO109I rG'GnC_Cy Cuts at: 2229 2449 2229 Size: 220 6198 EcoRI G'AATT_C Cuts at: 3465 3549 3727 4105 3465 Size: 84 178 378 5778 Fragments arranged by size: 5778 378 178 84 FspI TGC'GCA Cuts at: 4924 4924 Size: 6418 HaeII r_GCGC'y Cuts at: 1678 2206 5565 1678 Size: 528 3359 2531 Fragments arranged by size: 3359 2531 528 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2433 2465 2482 2514 2870 Size: 32 135 32 861 32 17 32 356 Cuts at: 2870 2902 3526 3558 5059 5091 5133 5165 1373 Size: 32 624 32 1501 32 42 32 2626 Fragments arranged by size: 2626 1501 861 624 356 135 42 32 32 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 2853 3762 4008 669 Size: 988 281 915 909 246 3079 Fragments arranged by size: 3079 988 915 909 281 246 HindIII A'AGCT_T Cuts at: 748 3091 3510 748 Size: 2343 419 3656 Fragments arranged by size: 3656 2343 419 HpaI GTT'AAC Cuts at: 4008 4008 Size: 6418 KpnI G_GTAC'C Cuts at: 3743 3743 Size: 6418 MfeI C'AATT_G Cuts at: 4017 4017 Size: 6418 MluI A'CGCG_T Cuts at: 6294 6294 Size: 6418 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3132 3300 5532 5716 1305 Size: 1827 168 2232 184 2007 Fragments arranged by size: 2232 2007 1827 184 168 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4596 Fragments arranged by size: 4596 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2720 3156 4376 4735 4894 518 Size: 648 1554 436 1220 359 159 2042 Fragments arranged by size: 2042 1554 1220 648 436 359 159 MspA1I CmG'CkG Cuts at: 1606 2684 3834 4511 5659 5904 1606 Size: 1078 1150 677 1148 245 2120 Fragments arranged by size: 2120 1150 1148 1078 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5866 NdeI CA'TA_TG Cuts at: 387 387 Size: 6418 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6418 NotI GC'GGCC_GC Cuts at: 3865 3865 Size: 6418 NsiI A_TGCA'T Cuts at: 3685 3685 Size: 6418 NspI r_CATG'y Cuts at: 1132 1229 2134 3776 5321 1132 Size: 97 905 1642 1545 2229 Fragments arranged by size: 2229 1642 1545 905 97 PasI CC'CwG_GG Cuts at: 1348 2818 1348 Size: 1470 4948 PciI A'CATG_T Cuts at: 2130 5317 2130 Size: 3187 3231 PflMI CCAn_nnn'nTGG Cuts at: 1108 2662 2698 1108 Size: 1554 36 4828 Fragments arranged by size: 4828 1554 36 PfoI T'CCnGG_A Cuts at: 2307 2307 Size: 6418 PmeI GTTT'AAAC Cuts at: 3722 3722 Size: 6418 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3133 3165 4487 4519 6000 6032 3133 Size: 32 1322 32 1481 32 3519 Fragments arranged by size: 3519 1481 1322 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2449 2449 Size: 6418 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6418 PsiI TTA'TAA Cuts at: 3988 3988 Size: 6418 PspOMI G'GGCC_C Cuts at: 2228 2228 Size: 6418 PspXI vC'TCGA_Gb Cuts at: 2521 2521 Size: 6418 PstI C_TGCA'G Cuts at: 830 2373 3770 830 Size: 1543 1397 3478 Fragments arranged by size: 3478 1543 1397 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6418 SacI G_AGCT'C Cuts at: 2358 2605 3107 3443 2358 Size: 247 502 336 5333 Fragments arranged by size: 5333 502 336 247 SalI G'TCGA_C Cuts at: 1936 3760 1936 Size: 1824 4594 SapI GCTCTTCn'nnn_ Cuts at: 2610 2610 Size: 6418 SbfI CC_TGCA'GG Cuts at: 3770 3770 Size: 6418 ScaI AGT'ACT Cuts at: 4666 6378 4666 Size: 1712 4706 SfcI C'TryA_G Cuts at: 826 1045 2153 2369 3021 3766 4901 5582 5773 Size: 219 1108 216 652 745 1135 681 191 Cuts at: 5773 826 Size: 1471 Fragments arranged by size: 1471 1135 1108 745 681 652 219 216 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6418 SmaI CCC'GGG Cuts at: 1872 3745 1872 Size: 1873 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2521 3558 4522 5423 5685 820 Size: 197 926 578 1037 964 901 262 1553 Fragments arranged by size: 1553 1037 964 926 901 578 262 197 SnaBI TAC'GTA Cuts at: 493 3715 4209 493 Size: 3222 494 2702 Fragments arranged by size: 3222 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6418 SphI G_CATG'C Cuts at: 1132 3776 1132 Size: 2644 3774 SspI AAT'ATT Cuts at: 5 52 4342 5 Size: 47 4290 2081 Fragments arranged by size: 4290 2081 47 StuI AGG'CCT Cuts at: 1517 3344 3428 3596 1517 Size: 1827 84 168 4339 Fragments arranged by size: 4339 1827 168 84 StyI C'CwwG_G Cuts at: 513 1065 2295 2349 3062 3857 513 Size: 552 1230 54 713 795 3074 Fragments arranged by size: 3074 1230 795 713 552 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2564 4445 4462 4615 4800 5245 325 Size: 1255 984 1881 17 153 185 445 1498 Fragments arranged by size: 1881 1498 1255 984 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3660 4664 6376 Size: 274 80 33 51 1443 1683 1004 1712 Cuts at: 6376 96 Size: 138 Fragments arranged by size: 1712 1683 1443 1004 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2152 2184 6378 6410 2152 Size: 32 4194 32 2160 Fragments arranged by size: 4194 2160 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6418 XbaI T'CTAG_A Cuts at: 2413 3754 2413 Size: 1341 5077 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2222 2921 2222 Size: 699 5719 XhoI C'TCGA_G Cuts at: 1943 2521 3558 1943 Size: 578 1037 4803 Fragments arranged by size: 4803 1037 578 XmaI C'CCGG_G Cuts at: 1870 3743 1870 Size: 1873 4545 XmnI GAAnn'nnTTC Cuts at: 3129 3260 3428 4547 3129 Size: 131 168 1119 5000 Fragments arranged by size: 5000 1119 168 131 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4226 276 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI