Restriction map for
pFN21ASDF0183
(Circular) (Six-base) MAPSORT of: pFN21ASDF0183.seq Check: 7404 from: 1 to: 5407
pFN21ASDF0183 5407 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2299 2320 2299
Size: 21 5386
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2454 3217 278
Size: 53 83 186 936 918 763 2468
Fragments arranged by size:
2468 936 918 763 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 3470 4854 5122 1632
Size: 986 852 1384 268 1917
Fragments arranged by size:
1917 1384 986 852 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 4306 5283 1203
Size: 3103 977 1327
Fragments arranged by size:
3103 1327 977
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5407
AlwNI CAG_nnn'CTG
Cuts at: 3150 4722 3150
Size: 1572 3835
ApaLI G'TGCA_C
Cuts at: 3464 4620 3464
Size: 1156 4251
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2732 1870
Size: 73 146 643 4545
Fragments arranged by size:
4545 643 146 73
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2728 4183 618
Size: 325 217 627 297 644 1455 1842
Fragments arranged by size:
1842 1455 644 627 325 297 217
BanII G_rGCy'C
Cuts at: 1391 2274 2726 1391
Size: 883 452 4072
Fragments arranged by size:
4072 883 452
BbeI G_GCGC'C
Cuts at: 2088 2088
Size: 5407
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3598 3632 1903
Size: 34 1661 34 3678
Fragments arranged by size:
3678 1661 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2300 3299 4509 2300
Size: 999 1210 3198
Fragments arranged by size:
3198 1210 999
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4018 136
Size: 107 122 71 631 605 2346 1525
Fragments arranged by size:
2346 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5402 5402
Size: 5407
BlpI GC'TnA_GC
Cuts at: 2824 2824
Size: 5407
Bme1580I G_kGCm'C
Cuts at: 1790 3468 4624 1790
Size: 1678 1156 2573
Fragments arranged by size:
2573 1678 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5407
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4096 442
Size: 416 53 83 399 24 1721 958 1753
Fragments arranged by size:
1753 1721 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4067 1239
Size: 2828 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3532 4397 4695 3532
Size: 865 298 4244
Fragments arranged by size:
4244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2237 4070 882
Size: 617 738 1833 2219
Fragments arranged by size:
2219 1833 738 617
BsaAI yAC'GTr
Cuts at: 493 1204 2704 3198 493
Size: 711 1500 494 2702
Fragments arranged by size:
2702 1500 711 494
BsaBI GATnn'nnATC
Cuts at: 5401 5401
Size: 5407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2451 3214 3596
Size: 53 83 186 936 552 366 763 382
Cuts at: 3596 4231 275
Size: 635 1451
Fragments arranged by size:
1451 936 763 635 552 382 366 186
83 53
BsaWI w'CCGG_w
Cuts at: 3840 4238 4512 4659 5070 3840
Size: 398 274 147 411 4177
Fragments arranged by size:
4177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2375 2405 1178
Size: 30 165 30 972 30 4180
Fragments arranged by size:
4180 972 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5407
BseYI C'CCAG_C
Cuts at: 4610 5106 4610
Size: 496 4911
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4834
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 2286 2857 3618 4646 1540
Size: 275 186 81 204 571 761 1028 2301
Fragments arranged by size:
2301 1028 761 571 275 204 186 81
BsiHKAI G_wGCw'C
Cuts at: 2160 2274 3468 3553 4624 2160
Size: 114 1194 85 1071 2943
Fragments arranged by size:
2943 1194 1071 114 85
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2274 2726 3468 3553 4624 1391
Size: 399 370 114 452 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 452 399 370 114 85
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2299 2320 2762 844
Size: 1455 21 442 3489
Fragments arranged by size:
3489 1455 442 21
BsrBI CCG'CTC
Cuts at: 1394 1823 2223 3294 1394
Size: 429 400 1071 3507
Fragments arranged by size:
3507 1071 429 400
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4076 65
Size: 1183 2654 174 1396
Fragments arranged by size:
2654 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 2285 4051 4238 1700
Size: 110 271 204 1766 187 2869
Fragments arranged by size:
2869 1766 271 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5407
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5407
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2125 794
Size: 1331 4076
BstBI TT'CG_AA
Cuts at: 2007 4211 2007
Size: 2204 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2093 2737 3104 3489 3506 4958 5044 5056
Size: 121 644 367 385 17 1452 86 12
Cuts at: 5056 5402 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 644 385 367 346 121 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2110 2110
Size: 5407
BtgI C'CryG_G
Cuts at: 513 1065 2022 2070 2316 2379 513
Size: 552 957 48 246 63 3541
Fragments arranged by size:
3541 957 552 246 63 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 3717 3737 2923
Size: 794 20 4593
Fragments arranged by size:
4593 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5372
DraI TTT'AAA
Cuts at: 2538 2711 2538
Size: 173 5234
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4414 809
Size: 507 3098 1802
Fragments arranged by size:
3098 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2283 2313
Size: 54 1425 50 275 18 249 204 30
Cuts at: 2313 2854 3743 5372 8
Size: 541 889 1629 43
Fragments arranged by size:
1629 1425 889 541 275 249 204 54
50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2079 2283 2854 1537
Size: 275 267 204 571 4090
Fragments arranged by size:
4090 571 275 267 204
EarI CTCTTCn'nnn_
Cuts at: 1353 2421 3337 1353
Size: 1068 916 3423
Fragments arranged by size:
3423 1068 916
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2288 2573 3994 4368 4514 387
Size: 1064 138 699 285 1421 374 146 1280
Fragments arranged by size:
1421 1280 1064 699 374 285 146 138
EcoICRI GAG'CTC
Cuts at: 2272 2272
Size: 5407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2618 3470 4067 4854 5122 1239
Size: 393 986 852 597 787 268 1524
Fragments arranged by size:
1524 986 852 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2306 2306
Size: 5407
EcoO109I rG'GnC_Cy
Cuts at: 2431 2431
Size: 5407
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 2088 4554 1678
Size: 410 2466 2531
Fragments arranged by size:
2531 2466 410
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 2556 2588 4048
Size: 32 135 32 803 32 149 32 1460
Cuts at: 4048 4080 4122 4154 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1460 803 149 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 2997 669
Size: 988 281 813 246 3079
Fragments arranged by size:
3079 988 813 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KasI G'GCGC_C
Cuts at: 2084 2084
Size: 5407
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4521 4705 1305
Size: 3216 184 2007
Fragments arranged by size:
3216 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2315 10
Size: 54 1425 343 483 3102
Fragments arranged by size:
3102 1425 483 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 3365 3724 3883 518
Size: 648 1293 906 359 159 2042
Fragments arranged by size:
2042 1293 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2072 2078 2344 2482 2823 3500 4648 4893
Size: 466 6 266 138 341 677 1148 245
Cuts at: 4893 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 466 341 266 245 138
6
NaeI GCC'GGC
Cuts at: 2083 2083
Size: 5407
NarI GG'CG_CC
Cuts at: 2085 2085
Size: 5407
NcoI C'CATG_G
Cuts at: 513 1065 2022 2316 2379 513
Size: 552 957 294 63 3541
Fragments arranged by size:
3541 957 552 294 63
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NgoMIV G'CCGG_C
Cuts at: 2081 2081
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2390 2765 4310 1132
Size: 97 1161 375 1545 2229
Fragments arranged by size:
2229 1545 1161 375 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5407
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2665 1108
Size: 1557 3850
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 4989 5021 3476
Size: 32 1481 32 3862
Fragments arranged by size:
3862 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2552 2977 2552
Size: 425 4982
PstI C_TGCA'G
Cuts at: 830 2056 2759 830
Size: 1226 703 3478
Fragments arranged by size:
3478 1226 703
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
PvuII CAG'CTG
Cuts at: 2482 2482
Size: 5407
SacI G_AGCT'C
Cuts at: 2274 2274
Size: 5407
SacII CC_GC'GG
Cuts at: 2073 2073
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SexAI A'CCwGG_T
Cuts at: 2664 2664
Size: 5407
SfcI C'TryA_G
Cuts at: 826 1045 2052 2755 3890 4571 4762 826
Size: 219 1007 703 1135 681 191 1471
Fragments arranged by size:
1471 1135 1007 703 681 219 191
SfoI GGC'GCC
Cuts at: 2086 2086
Size: 5407
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2091 2734 1872
Size: 219 643 4545
Fragments arranged by size:
4545 643 219
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3511 4412 4674 820
Size: 197 926 1568 901 262 1553
Fragments arranged by size:
1568 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2704 3198 493
Size: 2211 494 2702
Fragments arranged by size:
2702 2211 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5407
StyI C'CwwG_G
Cuts at: 513 1065 2022 2166 2316 2379 2846 513
Size: 552 957 144 150 63 467 3074
Fragments arranged by size:
3074 957 552 467 150 144 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 3434 3451 3604 3789 4234 325
Size: 1255 681 1173 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1173 681 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 3653 5365
Size: 274 80 33 51 1443 168 1508 1712
Cuts at: 5365 96
Size: 138
Fragments arranged by size:
1712 1508 1443 274 168 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2399 2431 5367 5399 2399
Size: 32 2936 32 2407
Fragments arranged by size:
2936 2407 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5407
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2089 2732 1870
Size: 219 643 4545
Fragments arranged by size:
4545 643 219
XmnI GAAnn'nnTTC
Cuts at: 3536 3536
Size: 5407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2452 3215 276
Size: 53 83 186 936 918 763 2468
Fragments arranged by size:
2468 936 918 763 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI
SrfI SwaI XcmI