Restriction map for
pFN21ASDF0168
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspI AvrII BbeI BciVI BclI
BfaI BfrI BglII BlnI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstBI BstXI BstZ17I Bsu36I
ClaI DraI Eco47III Eco81I EcoRI
EcoRV FbaI HincII HindII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I RcaI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 1315 GGCGTC G[GA]^CG[CT]C
ApaI 743 GGGCCC GGGCC^C
Asp700I 637 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1598 TGGCCA TGG^CCA
BbsI 538 GAAGAC GAAGAC(2/6)
BcgI 1310 GCACCGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 538 GAAGAC GAAGAC(2/6)
BsaHI 1315 GGCGTC G[GA]^CG[CT]C
BsaI 904 GAGACC GGTCTC(1/5)
BspMI 800 ACCTGC ACCTGC(4/8)
BsrBI 966 GAGCGG CCGCTC(-3/-3)
BsrDI 1274 CATTGC GCAATG(2/0)
BssSI 1686 CACGAG CACGAG(-5/-1)
CpoI 969 CGGACCG CG^G[AT]CCG
DrdI 1402 GACTGGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 857 CCGTGG C^C[GA][CT]GG
EarI 1237 GAAGAG CTCTTC(1/4)
EcoNI 1620 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 105 ATGCAT ATGCA^T
FseI 668 GGCCGGCC GGCCGG^CC
Hin1I 1315 GGCGTC G[GA]^CG[CT]C
HindIII 1071 AAGCTT A^AGCTT
Ksp632I 1237 GAAGAG CTCTTC(1/4)
MluNI 1598 TGGCCA TGG^CCA
MscI 1598 TGGCCA TGG^CCA
NsiI 105 ATGCAT ATGCA^T
PflMI 384 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 767 GACTC GAGTC(4/5)
PshAI 1473 GACGGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 594 CTGCAG CTGCA^G
PvuI 1128 CGATCG CGAT^CG
RsrII 969 CGGACCG CG^G[AT]CCG
SacI 822 GAGCTC GAGCT^C
SfcI 594 CTGCAG C^T[GA][CT]AG
TfiI 924 GAATC G^A[AT]TC
TspRI 587 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 384 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 637 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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AflIII 431 ACACGT A^C[GA][CT]GT
1193 ACACGT
AhdI 245 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
691 GACGTGATGTC
Alw44I 445 GTGCAC G^TGCAC
889 GTGCAC
AlwNI 385 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
679 CAGCTTCTG
ApaLI 445 GTGCAC G^TGCAC
889 GTGCAC
AspEI 245 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
691 GACGTGATGTC
AviII 344 TGCGCA TGC^GCA
461 TGCGCA
BamHI 114 GGATCC G^GATCC
365 GGATCC
BanI 483 GGTGCC G^G[CT][GA]CC
1309 GGCACC
BbvCI 45 CCTCAGC CCTCAGC(-5/-2)
756 CCTCAGC
BlpI 231 GCTCAGC GC^T[ACGT]AGC
819 GCTGAGC
Bpu1102I 231 GCTCAGC GC^T[ACGT]AGC
819 GCTGAGC
BsmBI 689 GAGACG CGTCTC(1/5)
1471 GAGACG
BstAPI 882 GCACGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1041 GCACTACGTGC
BstEII 144 GGTGACC G^GT[ACGT]ACC
252 GGTCACC
BstPI 144 GGTGACC G^GT[ACGT]ACC
252 GGTCACC
BstYI 114 GGATCC [GA]^GATC[CT]
365 GGATCC
CelII 231 GCTCAGC GC^T[ACGT]AGC
819 GCTGAGC
EagI 667 CGGCCG C^GGCCG
671 CGGCCG
Eam1105I 245 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
691 GACGTGATGTC
EclXI 667 CGGCCG C^GGCCG
671 CGGCCG
Eco52I 667 CGGCCG C^GGCCG
671 CGGCCG
EcoO65I 144 GGTGACC G^GT[ACGT]ACC
252 GGTCACC
FspI 344 TGCGCA TGC^GCA
461 TGCGCA
HinfI 767 GACTC G^A[ACGT]TC
924 GAATC
MaeIII 145 GTGAC ^GT[ACGT]AC
253 GTCAC
MflI 114 GGATCC [GA]^GATC[CT]
365 GGATCC
PpuMI 797 GGGACCT [GA]G^G[AT]CC[CT]
1690 AGGACCT
PvuII 130 CAGCTG CAG^CTG
750 CAGCTG
SfiI 3 GGCCCCATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
735 GGCCCTCCGGGCC
SgrAI 1311 CACCGGCG C[GA]^CCGG[CT]G
1373 CGCCGGCG
SphI 107 GCATGC GCATG^C
347 GCATGC
Tsp45I 145 GTGAC ^GT[GC]AC
253 GTCAC
XhoII 114 GGATCC [GA]^GATC[CT]
365 GGATCC
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbrPI
BbvI BcnI BglI BmyI BpmI
BsaAI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PmaCI PmlI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TseI TthHB8I