(Circular) (Six-base) MAPSORT of: pFN21ASDF0168.seq Check: 1211 from: 1 to: 6430 pFN21ASDF0168 6430 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3773 1937 Size: 1836 4594 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 2972 2981 4493 5877 6145 1632 Size: 581 759 9 1512 1384 268 1917 Fragments arranged by size: 1917 1512 1384 759 581 268 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 2454 3216 5329 6306 1203 Size: 1251 762 2113 977 1327 Fragments arranged by size: 2113 1327 1251 977 762 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 2273 2719 5159 2273 Size: 446 2440 3544 Fragments arranged by size: 3544 2440 446 AleI CACnn'nnGTG Cuts at: 1166 3083 1166 Size: 1917 4513 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2632 1184 Size: 1448 4982 AlwNI CAG_nnn'CTG Cuts at: 2413 2707 4173 5745 2413 Size: 294 1466 1572 3098 Fragments arranged by size: 3098 1572 1466 294 ApaI G_GGCC'C Cuts at: 2770 2770 Size: 6430 ApaLI G'TGCA_C Cuts at: 2468 2912 4487 5643 2468 Size: 444 1575 1156 3255 Fragments arranged by size: 3255 1575 1156 444 ApoI r'AATT_y Cuts at: 1493 3739 3965 4117 6226 1493 Size: 2246 226 152 2109 1697 Fragments arranged by size: 2246 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2510 2669 3572 3755 1870 Size: 73 567 159 903 183 4545 Fragments arranged by size: 4545 903 567 183 159 73 BamHI G'GATC_C Cuts at: 2137 2388 3760 4127 2137 Size: 251 1372 367 4440 Fragments arranged by size: 4440 1372 367 251 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2506 3332 3751 5206 618 Size: 325 217 627 719 826 419 1455 1842 Fragments arranged by size: 1842 1455 826 719 627 419 325 217 BanII G_rGCy'C Cuts at: 1391 2181 2421 2770 2849 3749 1391 Size: 790 240 349 79 900 4072 Fragments arranged by size: 4072 900 790 349 240 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2568 928 Size: 971 669 4790 Fragments arranged by size: 4790 971 669 BbvCI CC'TCA_GC Cuts at: 2069 2780 2069 Size: 711 5719 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3322 3356 4621 4655 1903 Size: 34 1385 34 1265 34 3678 Fragments arranged by size: 3678 1385 1265 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BfrBI ATG'CAT Cuts at: 2130 2130 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2033 2765 3112 Size: 107 122 71 631 605 361 732 347 Cuts at: 3112 3378 5041 136 Size: 266 1663 1525 Fragments arranged by size: 1663 1525 732 631 605 361 347 266 122 107 71 BglII A'GATC_T Cuts at: 6425 6425 Size: 6430 BlpI GC'TnA_GC Cuts at: 2255 2843 3847 2255 Size: 588 1004 4838 Fragments arranged by size: 4838 1004 588 Bme1580I G_kGCm'C Cuts at: 1790 2472 2511 2770 2916 3582 4491 5647 1790 Size: 682 39 259 146 666 909 1156 2573 Fragments arranged by size: 2573 1156 909 682 666 259 146 39 BmgBI CAC'GTC Cuts at: 1318 2716 2971 3289 1318 Size: 1398 255 318 4459 Fragments arranged by size: 4459 1398 318 255 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2434 2621 4161 Size: 416 53 83 399 24 1017 187 1540 Cuts at: 4161 5119 442 Size: 958 1753 Fragments arranged by size: 1753 1540 1017 958 416 399 187 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 2777 3654 5090 1239 Size: 1067 471 877 1436 2579 Fragments arranged by size: 2579 1436 1067 877 471 Bpu10I CC'TnA_GC Cuts at: 2069 2684 2780 3092 2069 Size: 615 96 312 5407 Fragments arranged by size: 5407 615 312 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2144 2588 3271 4555 5420 5718 2144 Size: 444 683 1284 865 298 2856 Fragments arranged by size: 2856 1284 865 683 444 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2921 5093 882 Size: 617 1422 2172 2219 Fragments arranged by size: 2219 2172 1422 617 BsaAI yAC'GTr Cuts at: 493 1204 2457 2917 3070 3219 3727 4221 493 Size: 711 1253 460 153 149 508 494 2702 Fragments arranged by size: 2702 1253 711 508 494 460 153 149 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3339 4237 4619 5254 Size: 53 83 186 936 1806 898 382 635 Cuts at: 5254 275 Size: 1451 Fragments arranged by size: 1806 1451 936 898 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4863 5261 5535 5682 6093 4863 Size: 398 274 147 411 5200 Fragments arranged by size: 5200 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6205 Fragments arranged by size: 6205 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2057 3134 1186 Size: 850 21 1077 4482 Fragments arranged by size: 4482 1077 850 21 BseYI C'CCAG_C Cuts at: 2356 2375 2518 2817 3486 5633 6129 2356 Size: 19 143 299 669 2147 496 2657 Fragments arranged by size: 2657 2147 669 496 299 143 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2024 2720 2939 3047 1163 Size: 573 288 696 219 108 4546 Fragments arranged by size: 4546 696 573 288 219 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2693 2697 3154 3880 4641 5669 Size: 275 186 692 4 457 726 761 1028 Cuts at: 5669 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 726 692 457 275 186 4 BsiHKAI G_wGCw'C Cuts at: 2256 2472 2849 2916 3126 3468 4491 4576 5647 Size: 216 377 67 210 342 1023 85 1071 Cuts at: 5647 2256 Size: 3039 Fragments arranged by size: 3039 1071 1023 377 342 216 210 85 67 BsmI GAATG_Cn' Cuts at: 3941 4034 3941 Size: 93 6337 BsmBI CGTCTCn'nnnn_ Cuts at: 2706 3488 2706 Size: 782 5648 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2256 2421 2472 2511 2770 2849 Size: 399 391 75 165 51 39 259 79 Cuts at: 2849 2916 3126 3468 3582 3749 4491 4576 5647 Size: 67 210 342 114 167 742 85 1071 Cuts at: 5647 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 391 342 259 210 167 165 114 85 79 75 67 51 39 BspEI T'CCGG_A Cuts at: 6093 6093 Size: 6430 BspHI T'CATG_A Cuts at: 4319 6049 4319 Size: 1730 4700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2832 3785 844 Size: 1988 953 3489 Fragments arranged by size: 3489 1988 953 BsrBI CCG'CTC Cuts at: 1394 1823 2991 4317 1394 Size: 429 1168 1326 3507 Fragments arranged by size: 3507 1326 1168 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3296 4925 5099 65 Size: 1183 2048 1629 174 1396 Fragments arranged by size: 2048 1629 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2688 2692 2995 3073 3245 3335 3397 Size: 110 878 4 303 78 172 90 62 Cuts at: 3397 3458 5074 5261 1700 Size: 61 1616 187 2869 Fragments arranged by size: 2869 1616 878 303 187 172 110 90 78 62 61 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6430 BssHII G'CGCG_C Cuts at: 1925 2833 2835 3195 3517 1925 Size: 908 2 360 322 4838 Fragments arranged by size: 4838 908 360 322 2 BssSI C'ACGA_G Cuts at: 3709 4490 5502 3709 Size: 781 1012 4637 Fragments arranged by size: 4637 1012 781 BstAPI GCAn_nnn'nTGC Cuts at: 794 2911 3070 794 Size: 2117 159 4154 Fragments arranged by size: 4154 2117 159 BstBI TT'CG_AA Cuts at: 2007 5234 2007 Size: 3227 3203 BstEII G'GTnAC_C Cuts at: 1184 2167 2275 1184 Size: 983 108 5339 Fragments arranged by size: 5339 983 108 BstYI r'GATC_y Cuts at: 1972 2137 2388 3760 4127 4512 4529 5981 6067 Size: 165 251 1372 367 385 17 1452 86 Cuts at: 6067 6079 6425 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1372 385 367 346 251 165 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2880 513 Size: 552 957 858 4063 Fragments arranged by size: 4063 957 858 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2274 2642 2903 487 Size: 683 56 1048 368 261 4014 Fragments arranged by size: 4014 1048 683 368 261 56 BtsI GCAGTG_nn' Cuts at: 3946 4740 4760 3946 Size: 794 20 5616 Fragments arranged by size: 5616 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DraIII CAC_nnn'GTG Cuts at: 2476 2911 3070 2476 Size: 435 159 5836 Fragments arranged by size: 5836 435 159 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3431 5437 809 Size: 507 2115 2006 1802 Fragments arranged by size: 2115 2006 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2241 2690 Size: 54 1425 50 275 18 399 12 449 Cuts at: 2690 2694 3075 3136 3442 3564 3621 3877 4766 Size: 4 381 61 306 122 57 256 889 Cuts at: 4766 6395 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 449 399 381 306 275 256 122 61 57 54 50 43 18 12 4 EagI C'GGCC_G Cuts at: 1537 1812 2690 2694 3877 1537 Size: 275 878 4 1183 4090 Fragments arranged by size: 4090 1183 878 275 4 EarI CTCTTCn'nnn_ Cuts at: 1353 3255 4360 1353 Size: 1902 1105 3423 Fragments arranged by size: 3423 1902 1105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3371 5017 5391 5537 387 Size: 1064 138 1782 1646 374 146 1280 Fragments arranged by size: 1782 1646 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2847 2847 Size: 6430 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2306 2777 2972 2981 3654 4493 Size: 393 581 93 471 195 9 673 839 Cuts at: 4493 5090 5877 6145 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 839 787 673 597 581 471 393 268 195 93 9 EcoNI CCTnn'n_nnAGG Cuts at: 3647 3647 Size: 6430 EcoO109I rG'GnC_Cy Cuts at: 2410 2590 2758 2821 3105 3548 3714 2410 Size: 180 168 63 284 443 166 5126 Fragments arranged by size: 5126 443 284 180 168 166 63 EcoRI G'AATT_C Cuts at: 3739 4117 3739 Size: 378 6052 FseI GG_CCGG'CC Cuts at: 2696 2696 Size: 6430 FspI TGC'GCA Cuts at: 2369 2486 4936 2369 Size: 117 2450 3863 Fragments arranged by size: 3863 2450 117 FspAI rTGC'GCAy Cuts at: 2369 2369 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 2223 3053 3281 3404 3515 5577 1678 Size: 545 830 228 123 111 2062 2531 Fragments arranged by size: 2531 2062 830 545 228 123 111 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2259 2291 2705 2737 3143 Size: 32 135 32 687 32 414 32 406 Cuts at: 3143 3175 5071 5103 5145 5177 1373 Size: 32 1896 32 42 32 2626 Fragments arranged by size: 2626 1896 687 414 406 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3774 4020 669 Size: 988 281 1836 246 3079 Fragments arranged by size: 3079 1836 988 281 246 HindIII A'AGCT_T Cuts at: 748 3094 748 Size: 2346 4084 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3204 5544 5728 1305 Size: 1899 2340 184 2007 Fragments arranged by size: 2340 2007 1899 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3623 10 Size: 54 1425 343 1791 2817 Fragments arranged by size: 2817 1791 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2369 3083 3612 4388 4747 4906 518 Size: 648 1203 714 529 776 359 159 2042 Fragments arranged by size: 2042 1203 776 714 648 529 359 159 MspA1I CmG'CkG Cuts at: 1606 2155 2775 3028 3846 4523 5671 5916 1606 Size: 549 620 253 818 677 1148 245 2120 Fragments arranged by size: 2120 1148 818 677 620 549 253 245 NaeI GCC'GGC Cuts at: 2690 2694 3075 3247 3399 3460 2690 Size: 4 381 172 152 61 5660 Fragments arranged by size: 5660 381 172 152 61 4 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4921 Fragments arranged by size: 4921 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6430 NgoMIV G'CCGG_C Cuts at: 2688 2692 3073 3245 3397 3458 2688 Size: 4 381 172 152 61 5660 Fragments arranged by size: 5660 381 172 152 61 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NsiI A_TGCA'T Cuts at: 2132 2132 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 2134 2374 3043 3611 3788 5333 1132 Size: 97 905 240 669 568 177 1545 2229 Fragments arranged by size: 2229 1545 905 669 568 240 177 97 PasI CC'CwG_GG Cuts at: 1348 2896 1348 Size: 1548 4882 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 2413 1108 Size: 1305 5125 PfoI T'CCnGG_A Cuts at: 2283 2283 Size: 6430 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PmlI CAC'GTG Cuts at: 2457 2917 3219 2457 Size: 460 302 5668 Fragments arranged by size: 5668 460 302 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4499 4531 6012 6044 4499 Size: 32 1481 32 4885 Fragments arranged by size: 4885 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2821 3714 2821 Size: 893 5537 PshAI GACnn'nnGTC Cuts at: 1613 3500 1613 Size: 1887 4543 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspOMI G'GGCC_C Cuts at: 2766 2766 Size: 6430 PstI C_TGCA'G Cuts at: 830 2621 3782 830 Size: 1791 1161 3478 Fragments arranged by size: 3478 1791 1161 PvuI CG_AT'CG Cuts at: 2001 3154 2001 Size: 1153 5277 PvuII CAG'CTG Cuts at: 2155 2775 2155 Size: 620 5810 RsrII CG'GwC_CG Cuts at: 2993 2993 Size: 6430 SacI G_AGCT'C Cuts at: 2849 2849 Size: 6430 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 4678 6390 4678 Size: 1712 4718 SfcI C'TryA_G Cuts at: 826 1045 2617 3778 4913 5594 5785 826 Size: 219 1572 1161 1135 681 191 1471 Fragments arranged by size: 1572 1471 1161 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2033 2765 2033 Size: 732 5698 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 3335 3397 1700 Size: 1635 62 4733 Fragments arranged by size: 4733 1635 62 SmaI CCC'GGG Cuts at: 1872 3757 1872 Size: 1885 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 2603 3250 4534 5435 5697 Size: 197 926 216 444 647 1284 901 262 Cuts at: 5697 820 Size: 1553 Fragments arranged by size: 1553 1284 926 901 647 444 262 216 197 SnaBI TAC'GTA Cuts at: 493 3727 4221 493 Size: 3234 494 2702 Fragments arranged by size: 3234 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 2134 2374 3788 1132 Size: 1002 240 1414 3774 Fragments arranged by size: 3774 1414 1002 240 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6430 StyI C'CwwG_G Cuts at: 513 1065 2022 2247 3081 3676 3869 513 Size: 552 957 225 834 595 193 3074 Fragments arranged by size: 3074 957 834 595 552 225 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2453 2801 3478 4457 4474 4627 4812 Size: 1255 873 348 677 979 17 153 185 Cuts at: 4812 5257 325 Size: 445 1498 Fragments arranged by size: 1498 1255 979 873 677 445 348 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2796 4676 6388 Size: 274 80 33 51 1443 819 1880 1712 Cuts at: 6388 96 Size: 138 Fragments arranged by size: 1880 1712 1443 819 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2985 3017 6390 6422 2985 Size: 32 3373 32 2993 Fragments arranged by size: 3373 2993 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6430 XbaI T'CTAG_A Cuts at: 3766 3766 Size: 6430 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2029 Size: 6430 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6430 XmaI C'CCGG_G Cuts at: 1870 3755 1870 Size: 1885 4545 XmnI GAAnn'nnTTC Cuts at: 2664 4559 2664 Size: 1895 4535 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI KasI NarI NruI PacI PspXI PsrI SacII SanDI SapI SexAI SfoI SrfI SwaI