Restriction map for
pFN21ASDF0168
(Linear) (Six-base) MAPSORT of: FLJ00168.seq Check: 8262 from: 1 to: 1701
FLJ00168.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 949 958 1701
Size: 190 759 9 743
Fragments arranged by size:
759 743 190 9
AflIII A'CryG_T
Cuts at: 0 431 1193 1701
Size: 431 762 508
Fragments arranged by size:
762 508 431
AhdI GACnn_n'nnGTC
Cuts at: 0 250 696 1701
Size: 250 446 1005
Fragments arranged by size:
1005 446 250
AleI CACnn'nnGTG
Cuts at: 0 1060 1701
Size: 1060 641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 609 1701
Size: 609 1092
AlwNI CAG_nnn'CTG
Cuts at: 0 390 684 1701
Size: 390 294 1017
Fragments arranged by size:
1017 390 294
ApaI G_GGCC'C
Cuts at: 0 747 1701
Size: 747 954
ApaLI G'TGCA_C
Cuts at: 0 445 889 1701
Size: 445 444 812
Fragments arranged by size:
812 445 444
AvaI C'yCGr_G
Cuts at: 0 487 646 1549 1701
Size: 487 159 903 152
Fragments arranged by size:
903 487 159 152
BamHI G'GATC_C
Cuts at: 0 114 365 1701
Size: 114 251 1336
Fragments arranged by size:
1336 251 114
BanI G'GyrC_C
Cuts at: 0 483 1309 1701
Size: 483 826 392
Fragments arranged by size:
826 483 392
BanII G_rGCy'C
Cuts at: 0 158 398 747 826 1701
Size: 158 240 349 79 875
Fragments arranged by size:
875 349 240 158 79
BbsI GAAGACnn'nnnn_
Cuts at: 0 545 1701
Size: 545 1156
BbvCI CC'TCA_GC
Cuts at: 0 46 757 1701
Size: 46 711 944
Fragments arranged by size:
944 711 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1299 1333 1701
Size: 1299 34 368
Fragments arranged by size:
1299 368 34
BfrBI ATG'CAT
Cuts at: 0 107 1701
Size: 107 1594
BglI GCCn_nnn'nGGC
Cuts at: 0 10 742 1089 1355 1701
Size: 10 732 347 266 346
Fragments arranged by size:
732 347 346 266 10
BlpI GC'TnA_GC
Cuts at: 0 232 820 1701
Size: 232 588 881
Fragments arranged by size:
881 588 232
Bme1580I G_kGCm'C
Cuts at: 0 449 488 747 893 1559 1701
Size: 449 39 259 146 666 142
Fragments arranged by size:
666 449 259 146 142 39
BmgBI CAC'GTC
Cuts at: 0 693 948 1266 1701
Size: 693 255 318 435
Fragments arranged by size:
693 435 318 255
BmrI ACTGGGnnnn_n'
Cuts at: 0 411 598 1701
Size: 411 187 1103
Fragments arranged by size:
1103 411 187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 754 1631 1701
Size: 283 471 877 70
Fragments arranged by size:
877 471 283 70
Bpu10I CC'TnA_GC
Cuts at: 0 46 661 757 1069 1701
Size: 46 615 96 312 632
Fragments arranged by size:
632 615 312 96 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 565 1248 1701
Size: 121 444 683 453
Fragments arranged by size:
683 453 444 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 898 1701
Size: 898 803
BsaAI yAC'GTr
Cuts at: 0 434 894 1047 1196 1701
Size: 434 460 153 149 505
Fragments arranged by size:
505 460 434 153 149
BsaHI Gr'CG_yC
Cuts at: 0 1316 1701
Size: 1316 385
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 13 34 1111 1701
Size: 13 21 1077 590
Fragments arranged by size:
1077 590 21 13
BseYI C'CCAG_C
Cuts at: 0 333 352 495 794 1463 1701
Size: 333 19 143 299 669 238
Fragments arranged by size:
669 333 299 238 143 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 697 916 1024 1701
Size: 1 696 219 108 677
Fragments arranged by size:
696 677 219 108 1
BsiEI CG_ry'CG
Cuts at: 0 670 674 1131 1701
Size: 670 4 457 570
Fragments arranged by size:
670 570 457 4
BsiHKAI G_wGCw'C
Cuts at: 0 233 449 826 893 1103 1445 1701
Size: 233 216 377 67 210 342 256
Fragments arranged by size:
377 342 256 233 216 210 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 683 1465 1701
Size: 683 782 236
Fragments arranged by size:
782 683 236
Bsp1286I G_dGCh'C
Cuts at: 0 158 233 398 449 488 747 826 893
Size: 158 75 165 51 39 259 79 67
Cuts at: 893 1103 1445 1559 1701
Size: 210 342 114 142
Fragments arranged by size:
342 259 210 165 158 142 114 79
75 67 51 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 809 1701
Size: 809 892
BsrBI CCG'CTC
Cuts at: 0 968 1701
Size: 968 733
BsrDI GCAATG_nn'
Cuts at: 0 1273 1701
Size: 1273 428
BsrFI r'CCGG_y
Cuts at: 0 665 669 972 1050 1222 1312 1374 1435
Size: 665 4 303 78 172 90 62 61
Cuts at: 1435 1701
Size: 266
Fragments arranged by size:
665 303 266 172 90 78 62 61
4
BssHII G'CGCG_C
Cuts at: 0 810 812 1172 1494 1701
Size: 810 2 360 322 207
Fragments arranged by size:
810 360 322 207 2
BssSI C'ACGA_G
Cuts at: 0 1686 1701
Size: 1686 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 888 1047 1701
Size: 888 159 654
Fragments arranged by size:
888 654 159
BstEII G'GTnAC_C
Cuts at: 0 144 252 1701
Size: 144 108 1449
Fragments arranged by size:
1449 144 108
BstYI r'GATC_y
Cuts at: 0 114 365 1701
Size: 114 251 1336
Fragments arranged by size:
1336 251 114
BtgI C'CryG_G
Cuts at: 0 857 1701
Size: 857 844
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 251 619 880 1701
Size: 251 368 261 821
Fragments arranged by size:
821 368 261 251
DraIII CAC_nnn'GTG
Cuts at: 0 453 888 1047 1701
Size: 453 435 159 654
Fragments arranged by size:
654 453 435 159
DrdI GACnn_nn'nnGTC
Cuts at: 0 1408 1701
Size: 1408 293
EaeI y'GGCC_r
Cuts at: 0 206 218 667 671 1052 1113 1419 1541
Size: 206 12 449 4 381 61 306 122
Cuts at: 1541 1598 1701
Size: 57 103
Fragments arranged by size:
449 381 306 206 122 103 61 57
12 4
EagI C'GGCC_G
Cuts at: 0 667 671 1701
Size: 667 4 1030
Fragments arranged by size:
1030 667 4
EarI CTCTTCn'nnn_
Cuts at: 0 1232 1701
Size: 1232 469
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1348 1701
Size: 1348 353
EcoICRI GAG'CTC
Cuts at: 0 824 1701
Size: 824 877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 283 754 949 958 1631 1701
Size: 190 93 471 195 9 673 70
Fragments arranged by size:
673 471 195 190 93 70 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1624 1701
Size: 1624 77
EcoO109I rG'GnC_Cy
Cuts at: 0 387 567 735 798 1082 1525 1691 1701
Size: 387 180 168 63 284 443 166 10
Fragments arranged by size:
443 387 284 180 168 166 63 10
FseI GG_CCGG'CC
Cuts at: 0 673 1701
Size: 673 1028
FspI TGC'GCA
Cuts at: 0 346 463 1701
Size: 346 117 1238
Fragments arranged by size:
1238 346 117
FspAI rTGC'GCAy
Cuts at: 0 346 1701
Size: 346 1355
HaeII r_GCGC'y
Cuts at: 0 200 1030 1258 1381 1492 1701
Size: 200 830 228 123 111 209
Fragments arranged by size:
830 228 209 200 123 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 236 268 682 714 1120 1152 1701
Size: 236 32 414 32 406 32 549
Fragments arranged by size:
549 414 406 236 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1071 1701
Size: 1071 630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1181 1701
Size: 1181 520
MscI TGG'CCA
Cuts at: 0 1600 1701
Size: 1600 101
MslI CAynn'nnrTG
Cuts at: 0 346 1060 1589 1701
Size: 346 714 529 112
Fragments arranged by size:
714 529 346 112
MspA1I CmG'CkG
Cuts at: 0 132 752 1005 1701
Size: 132 620 253 696
Fragments arranged by size:
696 620 253 132
NaeI GCC'GGC
Cuts at: 0 667 671 1052 1224 1376 1437 1701
Size: 667 4 381 172 152 61 264
Fragments arranged by size:
667 381 264 172 152 61 4
NgoMIV G'CCGG_C
Cuts at: 0 665 669 1050 1222 1374 1435 1701
Size: 665 4 381 172 152 61 266
Fragments arranged by size:
665 381 266 172 152 61 4
NsiI A_TGCA'T
Cuts at: 0 109 1701
Size: 109 1592
NspI r_CATG'y
Cuts at: 0 111 351 1020 1588 1701
Size: 111 240 669 568 113
Fragments arranged by size:
669 568 240 113 111
PasI CC'CwG_GG
Cuts at: 0 873 1701
Size: 873 828
PflMI CCAn_nnn'nTGG
Cuts at: 0 390 1701
Size: 390 1311
PfoI T'CCnGG_A
Cuts at: 0 260 1701
Size: 260 1441
PmlI CAC'GTG
Cuts at: 0 434 894 1196 1701
Size: 434 460 302 505
Fragments arranged by size:
505 460 434 302
PpuMI rG'GwC_Cy
Cuts at: 0 798 1691 1701
Size: 798 893 10
Fragments arranged by size:
893 798 10
PshAI GACnn'nnGTC
Cuts at: 0 1477 1701
Size: 1477 224
PspOMI G'GGCC_C
Cuts at: 0 743 1701
Size: 743 958
PstI C_TGCA'G
Cuts at: 0 598 1701
Size: 598 1103
PvuI CG_AT'CG
Cuts at: 0 1131 1701
Size: 1131 570
PvuII CAG'CTG
Cuts at: 0 132 752 1701
Size: 132 620 949
Fragments arranged by size:
949 620 132
RsrII CG'GwC_CG
Cuts at: 0 970 1701
Size: 970 731
SacI G_AGCT'C
Cuts at: 0 826 1701
Size: 826 875
SfcI C'TryA_G
Cuts at: 0 594 1701
Size: 594 1107
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 10 742 1701
Size: 10 732 959
Fragments arranged by size:
959 732 10
SgrAI Cr'CCGG_yG
Cuts at: 0 1312 1374 1701
Size: 1312 62 327
Fragments arranged by size:
1312 327 62
SmlI C'TyrA_G
Cuts at: 0 136 580 1227 1701
Size: 136 444 647 474
Fragments arranged by size:
647 474 444 136
SphI G_CATG'C
Cuts at: 0 111 351 1701
Size: 111 240 1350
Fragments arranged by size:
1350 240 111
StyI C'CwwG_G
Cuts at: 0 224 1058 1653 1701
Size: 224 834 595 48
Fragments arranged by size:
834 595 224 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 430 778 1455 1701
Size: 430 348 677 246
Fragments arranged by size:
677 430 348 246
TatI w'GTAC_w
Cuts at: 0 773 1701
Size: 773 928
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 962 994 1701
Size: 962 32 707
Fragments arranged by size:
962 707 32
XmnI GAAnn'nnTTC
Cuts at: 0 641 1701
Size: 641 1060
Enzymes that do cut:
AcuI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstEII BstYI BtgI BtgZI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI
FspI FspAI HaeII Hin4I HindIII MmeI MscI MslI
MspA1I NaeI NgoMIV NsiI NspI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SfcI SfiI SgrAI SmlI SphI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BciVI BclI BglII BmtI BplI BsaBI BsaWI BsaXI
BsiWI BsmI BspEI BspHI BsrGI BstBI BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DraI EcoRI EcoRV FalI
HincII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NruI PacI PciI PmeI PpiI
PsiI PspXI PsrI SacII SalI SanDI SapI SbfI
ScaI SexAI SfoI SgfI SmaI SnaBI SpeI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI