(Circular) (Six-base) MAPSORT of: pFN21ASDF0136.seq Check: 7252 from: 1 to: 7144 pFN21ASDF0136 7144 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3092 4033 4070 3092 Size: 941 37 6166 Fragments arranged by size: 6166 941 37 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4954 278 Size: 53 83 186 936 3418 2468 Fragments arranged by size: 3418 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4487 1937 Size: 2550 4594 Acc65I G'GTAC_C Cuts at: 4170 4465 4170 Size: 295 6849 AclI AA'CG_TT Cuts at: 1563 5271 5644 1563 Size: 3708 373 3063 Fragments arranged by size: 3708 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2744 3266 3779 5207 6591 6859 1632 Size: 1112 522 513 1428 1384 268 1917 Fragments arranged by size: 1917 1428 1384 1112 522 513 268 AflII C'TTAA_G Cuts at: 820 1017 2530 820 Size: 197 1513 5434 Fragments arranged by size: 5434 1513 197 AflIII A'CryG_T Cuts at: 1203 6043 7020 1203 Size: 4840 977 1327 Fragments arranged by size: 4840 1327 977 AgeI A'CCGG_T Cuts at: 3672 4167 5975 3672 Size: 495 1808 4841 Fragments arranged by size: 4841 1808 495 AhdI GACnn_n'nnGTC Cuts at: 5873 5873 Size: 7144 AleI CACnn'nnGTG Cuts at: 1166 3095 1166 Size: 1929 5215 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7144 AlwNI CAG_nnn'CTG Cuts at: 2035 2488 2701 3416 3667 3826 4887 6459 2035 Size: 453 213 715 251 159 1061 1572 2720 Fragments arranged by size: 2720 1572 1061 715 453 251 213 159 ApaI G_GGCC'C Cuts at: 2087 2840 2873 3481 2087 Size: 753 33 608 5750 Fragments arranged by size: 5750 753 608 33 ApaLI G'TGCA_C Cuts at: 2111 2152 3004 4163 5201 6357 2111 Size: 41 852 1159 1038 1156 2898 Fragments arranged by size: 2898 1159 1156 1038 852 41 ApoI r'AATT_y Cuts at: 1493 4453 4679 4831 6940 1493 Size: 2960 226 152 2109 1697 Fragments arranged by size: 2960 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5698 160 Size: 872 4666 1606 Fragments arranged by size: 4666 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7144 AvaI C'yCGr_G Cuts at: 1870 1943 2231 2372 2575 4054 4469 1870 Size: 73 288 141 203 1479 415 4545 Fragments arranged by size: 4545 1479 415 288 203 141 73 BamHI G'GATC_C Cuts at: 4474 4841 4474 Size: 367 6777 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2237 2306 3934 4010 4170 Size: 325 217 627 450 69 1628 76 160 Cuts at: 4170 4402 4465 5920 618 Size: 232 63 1455 1842 Fragments arranged by size: 1842 1628 1455 627 450 325 232 217 160 76 69 63 BanII G_rGCy'C Cuts at: 1391 2087 2481 2840 2873 2901 3481 4463 1391 Size: 696 394 359 33 28 580 982 4072 Fragments arranged by size: 4072 982 696 580 394 359 33 28 BbeI G_GCGC'C Cuts at: 3938 4014 3938 Size: 76 7068 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3163 3376 3886 3914 3967 4012 4189 Size: 971 1264 213 510 28 53 45 177 Cuts at: 4189 928 Size: 3883 Fragments arranged by size: 3883 1264 971 510 213 177 53 45 28 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4379 4413 5335 5369 1903 Size: 34 2442 34 922 34 3678 Fragments arranged by size: 3678 2442 922 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5036 6246 5036 Size: 1210 5934 BclI T'GATC_A Cuts at: 1548 1548 Size: 7144 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2601 2696 2745 Size: 107 122 71 631 605 929 95 49 Cuts at: 2745 3943 5755 136 Size: 1198 1812 1525 Fragments arranged by size: 1812 1525 1198 929 631 605 122 107 95 71 49 BglII A'GATC_T Cuts at: 7139 7139 Size: 7144 BlpI GC'TnA_GC Cuts at: 4561 4561 Size: 7144 Bme1580I G_kGCm'C Cuts at: 1790 2087 2115 2156 2240 2840 2873 3008 3481 Size: 297 28 41 84 600 33 135 473 Cuts at: 3481 4167 5205 6361 1790 Size: 686 1038 1156 2573 Fragments arranged by size: 2573 1156 1038 686 600 473 297 135 84 41 33 28 BmgBI CAC'GTC Cuts at: 1318 3382 1318 Size: 2064 5080 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 4875 5833 Size: 416 53 83 399 24 897 2561 958 Cuts at: 5833 442 Size: 1753 Fragments arranged by size: 2561 1753 958 897 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7144 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2441 2462 2465 2734 2915 3203 3530 Size: 953 249 21 3 269 181 288 327 Cuts at: 3530 3554 3824 4145 5804 1239 Size: 24 270 321 1659 2579 Fragments arranged by size: 2579 1659 953 327 321 288 270 269 249 181 24 21 3 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2762 3554 4265 5269 6134 6432 2762 Size: 792 711 1004 865 298 3474 Fragments arranged by size: 3474 1004 865 792 711 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2054 4150 5807 882 Size: 617 555 2096 1657 2219 Fragments arranged by size: 2219 2096 1657 617 555 BsaAI yAC'GTr Cuts at: 493 1204 3544 4441 4935 493 Size: 711 2340 897 494 2702 Fragments arranged by size: 2702 2340 897 711 494 BsaBI GATnn'nnATC Cuts at: 7138 7138 Size: 7144 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3624 3935 4011 4951 Size: 53 83 186 936 2091 311 76 940 Cuts at: 4951 5333 5968 275 Size: 382 635 1451 Fragments arranged by size: 2091 1451 940 936 635 382 311 186 83 76 53 BsaWI w'CCGG_w Cuts at: 3672 4074 4167 5577 5975 6249 6396 6807 3672 Size: 402 93 1410 398 274 147 411 4009 Fragments arranged by size: 4009 1410 411 402 398 274 147 93 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6919 Fragments arranged by size: 6919 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2459 2462 2465 2891 3455 3778 3848 4379 Size: 1273 3 3 426 564 323 70 531 Cuts at: 4379 4384 4406 1186 Size: 5 22 3924 Fragments arranged by size: 3924 1273 564 531 426 323 70 22 5 3 3 BseYI C'CCAG_C Cuts at: 2028 2087 2279 2399 3322 3358 3607 3642 3835 Size: 59 192 120 923 36 249 35 193 Cuts at: 3835 6347 6843 2028 Size: 2512 496 2329 Fragments arranged by size: 2512 2329 923 496 249 193 192 120 59 36 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2360 2371 2869 3890 1163 Size: 573 624 11 498 1021 4417 Fragments arranged by size: 4417 1021 624 573 498 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4594 5355 6383 1540 Size: 275 186 2593 761 1028 2301 Fragments arranged by size: 2593 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2115 2156 2249 2481 2760 2901 3008 3846 3971 Size: 41 93 232 279 141 107 838 125 Cuts at: 3971 4061 4167 5205 5290 6361 2115 Size: 90 106 1038 85 1071 2898 Fragments arranged by size: 2898 1071 1038 838 279 232 141 125 107 106 93 90 85 41 BsmI GAATG_Cn' Cuts at: 4655 4748 4655 Size: 93 7051 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2115 2156 2240 2249 2481 2760 Size: 399 297 28 41 84 9 232 279 Cuts at: 2760 2840 2873 2901 3008 3481 3846 3971 4061 Size: 80 33 28 107 473 365 125 90 Cuts at: 4061 4167 4463 5205 5290 6361 1391 Size: 106 296 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 473 399 365 297 296 279 232 125 107 106 90 85 84 80 41 33 28 28 9 BspEI T'CCGG_A Cuts at: 4074 6807 4074 Size: 2733 4411 BspHI T'CATG_A Cuts at: 3396 5033 6763 3396 Size: 1637 1730 3777 Fragments arranged by size: 3777 1730 1637 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3092 4033 4070 4499 844 Size: 2248 941 37 429 3489 Fragments arranged by size: 3489 2248 941 429 37 BsrBI CCG'CTC Cuts at: 1394 1823 2191 2388 5031 1394 Size: 429 368 197 2643 3507 Fragments arranged by size: 3507 2643 429 368 197 BsrDI GCAATG_nn' Cuts at: 65 1248 3786 4303 5639 5813 65 Size: 1183 2538 517 1336 174 1396 Fragments arranged by size: 2538 1396 1336 1183 517 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2361 2631 3672 3942 4100 4167 5788 Size: 110 551 270 1041 270 158 67 1621 Cuts at: 5788 5975 1700 Size: 187 2869 Fragments arranged by size: 2869 1621 1041 551 270 270 187 158 110 67 BsrGI T'GTAC_A Cuts at: 96 3099 96 Size: 3003 4141 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7144 BssSI C'ACGA_G Cuts at: 5204 6216 5204 Size: 1012 6132 BstAPI GCAn_nnn'nTGC Cuts at: 794 3003 4409 794 Size: 2209 1406 3529 Fragments arranged by size: 3529 2209 1406 BstBI TT'CG_AA Cuts at: 2007 5948 2007 Size: 3941 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7144 BstXI CCAn_nnnn'nTGG Cuts at: 2440 2978 3919 2440 Size: 538 941 5665 Fragments arranged by size: 5665 941 538 BstYI r'GATC_y Cuts at: 1972 4474 4841 5226 5243 6695 6781 6793 7139 Size: 2502 367 385 17 1452 86 12 346 Cuts at: 7139 1972 Size: 1977 Fragments arranged by size: 2502 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2841 2841 Size: 7144 BtgI C'CryG_G Cuts at: 513 1065 2022 2433 2708 3369 513 Size: 552 957 411 275 661 4288 Fragments arranged by size: 4288 957 661 552 411 275 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6405 Fragments arranged by size: 6405 683 56 BtsI GCAGTG_nn' Cuts at: 2499 4660 5454 5474 2499 Size: 2161 794 20 4169 Fragments arranged by size: 4169 2161 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7144 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7109 DraI TTT'AAA Cuts at: 4448 4448 Size: 7144 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6151 809 Size: 507 4835 1802 Fragments arranged by size: 4835 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2268 2293 Size: 54 1425 50 275 18 420 18 25 Cuts at: 2293 2629 2944 3258 3372 3421 3501 3546 3576 Size: 336 315 314 114 49 80 45 30 Cuts at: 3576 3921 4221 4591 5480 7109 8 Size: 345 300 370 889 1629 43 Fragments arranged by size: 1629 1425 889 420 370 345 336 315 314 300 275 114 80 54 50 49 45 43 30 25 18 18 EagI C'GGCC_G Cuts at: 1537 1812 4591 1537 Size: 275 2779 4090 Fragments arranged by size: 4090 2779 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2558 3755 3851 5074 1353 Size: 1205 1197 96 1223 3423 Fragments arranged by size: 3423 1223 1205 1197 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2380 2396 2677 3784 5731 6105 Size: 1064 138 791 16 281 1107 1947 374 Cuts at: 6105 6251 387 Size: 146 1280 Fragments arranged by size: 1947 1280 1107 1064 791 374 281 146 138 16 EcoICRI GAG'CTC Cuts at: 2479 2899 2479 Size: 420 6724 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2441 2462 2465 2734 2744 2915 Size: 393 560 249 21 3 269 10 171 Cuts at: 2915 3203 3266 3530 3554 3779 3824 4145 5207 Size: 288 63 264 24 225 45 321 1062 Cuts at: 5207 5804 6591 6859 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1062 787 597 560 393 321 288 269 268 264 249 225 171 63 45 24 21 10 3 EcoNI CCTnn'n_nnAGG Cuts at: 2444 2471 3221 4280 2444 Size: 27 750 1059 5308 Fragments arranged by size: 5308 1059 750 27 EcoO109I rG'GnC_Cy Cuts at: 2083 2084 2571 2698 2837 2870 3477 3478 4136 Size: 1 487 127 139 33 607 1 658 Cuts at: 4136 2083 Size: 5091 Fragments arranged by size: 5091 658 607 487 139 127 33 1 1 EcoRI G'AATT_C Cuts at: 4453 4831 4453 Size: 378 6766 FspI TGC'GCA Cuts at: 5650 5650 Size: 7144 HaeII r_GCGC'y Cuts at: 1678 2681 2716 3938 4014 6291 1678 Size: 1003 35 1222 76 2277 2531 Fragments arranged by size: 2531 2277 1222 1003 76 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2295 2327 3391 3423 5785 Size: 32 135 32 723 32 1064 32 2362 Cuts at: 5785 5817 5859 5891 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2362 1064 723 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4488 4734 669 Size: 988 281 2550 246 3079 Fragments arranged by size: 3079 2550 988 281 246 HindIII A'AGCT_T Cuts at: 748 4331 748 Size: 3583 3561 HpaI GTT'AAC Cuts at: 4734 4734 Size: 7144 KasI G'GCGC_C Cuts at: 3934 4010 3934 Size: 76 7068 KpnI G_GTAC'C Cuts at: 4174 4469 4174 Size: 295 6849 MfeI C'AATT_G Cuts at: 4743 4743 Size: 7144 MluI A'CGCG_T Cuts at: 7020 7020 Size: 7144 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6258 6442 1305 Size: 4953 184 2007 Fragments arranged by size: 4953 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 3423 3548 3578 3923 Size: 54 1425 343 438 1153 125 30 345 Cuts at: 3923 10 Size: 3231 Fragments arranged by size: 3231 1425 1153 438 345 343 125 54 30 MslI CAynn'nnrTG Cuts at: 518 1166 2933 3095 4244 5102 5461 5620 518 Size: 648 1767 162 1149 858 359 159 2042 Fragments arranged by size: 2042 1767 1149 858 648 359 162 159 MspA1I CmG'CkG Cuts at: 1606 2503 2607 3271 4016 4560 5237 6385 6630 Size: 897 104 664 745 544 677 1148 245 Cuts at: 6630 1606 Size: 2120 Fragments arranged by size: 2120 1148 897 745 677 664 544 245 104 NaeI GCC'GGC Cuts at: 2363 2633 3944 4102 2363 Size: 270 1311 158 5405 Fragments arranged by size: 5405 1311 270 158 NarI GG'CG_CC Cuts at: 3935 4011 3935 Size: 76 7068 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5635 Fragments arranged by size: 5635 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7144 NgoMIV G'CCGG_C Cuts at: 2361 2631 3942 4100 2361 Size: 270 1311 158 5405 Fragments arranged by size: 5405 1311 270 158 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7144 NotI GC'GGCC_GC Cuts at: 4591 4591 Size: 7144 NspI r_CATG'y Cuts at: 1132 1229 2278 2515 3040 4502 6047 1132 Size: 97 1049 237 525 1462 1545 2229 Fragments arranged by size: 2229 1545 1462 1049 525 237 97 PasI CC'CwG_GG Cuts at: 1348 2066 3012 4140 4158 1348 Size: 718 946 1128 18 4334 Fragments arranged by size: 4334 1128 946 718 18 PciI A'CATG_T Cuts at: 6043 6043 Size: 7144 PflMI CCAn_nnn'nTGG Cuts at: 1108 2035 2488 3607 1108 Size: 927 453 1119 4645 Fragments arranged by size: 4645 1119 927 453 PfoI T'CCnGG_A Cuts at: 2136 3801 2136 Size: 1665 5479 PmeI GTTT'AAAC Cuts at: 4448 4448 Size: 7144 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5213 5245 6726 6758 5213 Size: 32 1481 32 5599 Fragments arranged by size: 5599 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2571 2571 Size: 7144 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7144 PsiI TTA'TAA Cuts at: 4714 4714 Size: 7144 PspOMI G'GGCC_C Cuts at: 2083 2836 2869 3477 2083 Size: 753 33 608 5750 Fragments arranged by size: 5750 753 608 33 PspXI vC'TCGA_Gb Cuts at: 4054 4054 Size: 7144 PstI C_TGCA'G Cuts at: 830 2181 2697 3557 3633 4496 830 Size: 1351 516 860 76 863 3478 Fragments arranged by size: 3478 1351 863 860 516 76 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7144 PvuII CAG'CTG Cuts at: 3271 3271 Size: 7144 SacI G_AGCT'C Cuts at: 2481 2901 2481 Size: 420 6724 SalI G'TCGA_C Cuts at: 1936 4486 1936 Size: 2550 4594 SapI GCTCTTCn'nnn_ Cuts at: 2558 3851 2558 Size: 1293 5851 SbfI CC_TGCA'GG Cuts at: 2697 3633 4496 2697 Size: 936 863 5345 Fragments arranged by size: 5345 936 863 ScaI AGT'ACT Cuts at: 2324 5392 7104 2324 Size: 3068 1712 2364 Fragments arranged by size: 3068 2364 1712 SfcI C'TryA_G Cuts at: 826 1045 2177 2693 2950 3553 3629 4492 5627 Size: 219 1132 516 257 603 76 863 1135 Cuts at: 5627 6308 6499 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1132 863 681 603 516 257 219 191 76 SfoI GGC'GCC Cuts at: 3936 4012 3936 Size: 76 7068 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7144 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7144 SmaI CCC'GGG Cuts at: 1872 4471 1872 Size: 2599 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2530 2777 3569 4054 4280 5248 Size: 197 926 587 247 792 485 226 968 Cuts at: 5248 6149 6411 820 Size: 901 262 1553 Fragments arranged by size: 1553 968 926 901 792 587 485 262 247 226 197 SnaBI TAC'GTA Cuts at: 493 4441 4935 493 Size: 3948 494 2702 Fragments arranged by size: 3948 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7144 SphI G_CATG'C Cuts at: 1132 2278 4502 1132 Size: 1146 2224 3774 Fragments arranged by size: 3774 2224 1146 SspI AAT'ATT Cuts at: 5 52 3232 5068 5 Size: 47 3180 1836 2081 Fragments arranged by size: 3180 2081 1836 47 StuI AGG'CCT Cuts at: 1517 2892 1517 Size: 1375 5769 StyI C'CwwG_G Cuts at: 513 1065 2022 4583 513 Size: 552 957 2561 3074 Fragments arranged by size: 3074 2561 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2255 2939 3008 3025 4102 4150 5171 Size: 1255 675 684 69 17 1077 48 1021 Cuts at: 5171 5188 5341 5526 5971 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1077 1021 684 675 445 185 153 69 48 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 2937 3099 Size: 274 80 33 51 1443 345 615 162 Cuts at: 3099 5390 7102 96 Size: 2291 1712 138 Fragments arranged by size: 2291 1712 1443 615 345 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2247 2279 3716 3748 7104 7136 2247 Size: 32 1437 32 3356 32 2255 Fragments arranged by size: 3356 2255 1437 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3692 1316 Size: 2376 4768 XbaI T'CTAG_A Cuts at: 4480 4480 Size: 7144 XhoI C'TCGA_G Cuts at: 1943 4054 1943 Size: 2111 5033 XmaI C'CCGG_G Cuts at: 1870 4469 1870 Size: 2599 4545 XmnI GAAnn'nnTTC Cuts at: 5273 5273 Size: 7144 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4952 276 Size: 53 83 186 936 3418 2468 Fragments arranged by size: 3418 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI