Restriction map for
pFN21ASDF0136
(Linear) (Six-base) MAPSORT of: FLJ00136.seq Check: 956 from: 1 to: 2415
FLJ00136.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1069 2010 2047 2415
Size: 1069 941 37 368
Fragments arranged by size:
1069 941 368 37
Acc65I G'GTAC_C
Cuts at: 0 2147 2415
Size: 2147 268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1243 1756 2415
Size: 721 522 513 659
Fragments arranged by size:
721 659 522 513
AflII C'TTAA_G
Cuts at: 0 507 2415
Size: 507 1908
AgeI A'CCGG_T
Cuts at: 0 1649 2144 2415
Size: 1649 495 271
Fragments arranged by size:
1649 495 271
AleI CACnn'nnGTG
Cuts at: 0 1072 2415
Size: 1072 1343
AlwNI CAG_nnn'CTG
Cuts at: 0 12 465 678 1393 1644 1803 2415
Size: 12 453 213 715 251 159 612
Fragments arranged by size:
715 612 453 251 213 159 12
ApaI G_GGCC'C
Cuts at: 0 64 817 850 1458 2415
Size: 64 753 33 608 957
Fragments arranged by size:
957 753 608 64 33
ApaLI G'TGCA_C
Cuts at: 0 88 129 981 2140 2415
Size: 88 41 852 1159 275
Fragments arranged by size:
1159 852 275 88 41
AvaI C'yCGr_G
Cuts at: 0 208 349 552 2031 2415
Size: 208 141 203 1479 384
Fragments arranged by size:
1479 384 208 203 141
BanI G'GyrC_C
Cuts at: 0 214 283 1911 1987 2147 2379 2415
Size: 214 69 1628 76 160 232 36
Fragments arranged by size:
1628 232 214 160 76 69 36
BanII G_rGCy'C
Cuts at: 0 64 458 817 850 878 1458 2415
Size: 64 394 359 33 28 580 957
Fragments arranged by size:
957 580 394 359 64 33 28
BbeI G_GCGC'C
Cuts at: 0 1915 1991 2415
Size: 1915 76 424
Fragments arranged by size:
1915 424 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 1140 1353 1863 1891 1944 1989 2166 2415
Size: 1140 213 510 28 53 45 177 249
Fragments arranged by size:
1140 510 249 213 177 53 45 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2356 2390 2415
Size: 2356 34 25
Fragments arranged by size:
2356 34 25
BglI GCCn_nnn'nGGC
Cuts at: 0 578 673 722 1920 2415
Size: 578 95 49 1198 495
Fragments arranged by size:
1198 578 495 95 49
Bme1580I G_kGCm'C
Cuts at: 0 64 92 133 217 817 850 985 1458
Size: 64 28 41 84 600 33 135 473
Cuts at: 1458 2144 2415
Size: 686 271
Fragments arranged by size:
686 600 473 271 135 84 64 41
33 28
BmgBI CAC'GTC
Cuts at: 0 1359 2415
Size: 1359 1056
BmrI ACTGGGnnnn_n'
Cuts at: 0 291 2415
Size: 291 2124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 418 439 442 711 892 1180 1507
Size: 169 249 21 3 269 181 288 327
Cuts at: 1507 1531 1801 2122 2415
Size: 24 270 321 293
Fragments arranged by size:
327 321 293 288 270 269 249 181
169 24 21 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 1531 2242 2415
Size: 739 792 711 173
Fragments arranged by size:
792 739 711 173
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 2127 2415
Size: 31 2096 288
Fragments arranged by size:
2096 288 31
BsaAI yAC'GTr
Cuts at: 0 1521 2415
Size: 1521 894
BsaHI Gr'CG_yC
Cuts at: 0 1601 1912 1988 2415
Size: 1601 311 76 427
Fragments arranged by size:
1601 427 311 76
BsaWI w'CCGG_w
Cuts at: 0 1649 2051 2144 2415
Size: 1649 402 93 271
Fragments arranged by size:
1649 402 271 93
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 439 442 868 1432 1755 1825 2356
Size: 436 3 3 426 564 323 70 531
Cuts at: 2356 2361 2383 2415
Size: 5 22 32
Fragments arranged by size:
564 531 436 426 323 70 32 22
5 3 3
BseYI C'CCAG_C
Cuts at: 0 5 64 256 376 1299 1335 1584 1619
Size: 5 59 192 120 923 36 249 35
Cuts at: 1619 1812 2415
Size: 193 603
Fragments arranged by size:
923 603 249 193 192 120 59 36
35 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 348 846 1867 2415
Size: 337 11 498 1021 548
Fragments arranged by size:
1021 548 498 337 11
BsiHKAI G_wGCw'C
Cuts at: 0 92 133 226 458 737 878 985 1823
Size: 92 41 93 232 279 141 107 838
Cuts at: 1823 1948 2038 2144 2415
Size: 125 90 106 271
Fragments arranged by size:
838 279 271 232 141 125 107 106
93 92 90 41
Bsp1286I G_dGCh'C
Cuts at: 0 64 92 133 217 226 458 737 817
Size: 64 28 41 84 9 232 279 80
Cuts at: 817 850 878 985 1458 1823 1948 2038 2144
Size: 33 28 107 473 365 125 90 106
Cuts at: 2144 2415
Size: 271
Fragments arranged by size:
473 365 279 271 232 125 107 106
90 84 80 64 41 33 28 28
9
BspEI T'CCGG_A
Cuts at: 0 2051 2415
Size: 2051 364
BspHI T'CATG_A
Cuts at: 0 1373 2415
Size: 1373 1042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1069 2010 2047 2415
Size: 1069 941 37 368
Fragments arranged by size:
1069 941 368 37
BsrBI CCG'CTC
Cuts at: 0 168 365 2415
Size: 168 197 2050
Fragments arranged by size:
2050 197 168
BsrDI GCAATG_nn'
Cuts at: 0 1763 2280 2415
Size: 1763 517 135
Fragments arranged by size:
1763 517 135
BsrFI r'CCGG_y
Cuts at: 0 338 608 1649 1919 2077 2144 2415
Size: 338 270 1041 270 158 67 271
Fragments arranged by size:
1041 338 271 270 270 158 67
BsrGI T'GTAC_A
Cuts at: 0 1076 2415
Size: 1076 1339
BstAPI GCAn_nnn'nTGC
Cuts at: 0 980 2386 2415
Size: 980 1406 29
Fragments arranged by size:
1406 980 29
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 955 1896 2415
Size: 417 538 941 519
Fragments arranged by size:
941 538 519 417
Bsu36I CC'TnA_GG
Cuts at: 0 818 2415
Size: 818 1597
BtgI C'CryG_G
Cuts at: 0 410 685 1346 2415
Size: 410 275 661 1069
Fragments arranged by size:
1069 661 410 275
BtsI GCAGTG_nn'
Cuts at: 0 476 2415
Size: 476 1939
EaeI y'GGCC_r
Cuts at: 0 227 245 270 606 921 1235 1349 1398
Size: 227 18 25 336 315 314 114 49
Cuts at: 1398 1478 1523 1553 1898 2198 2415
Size: 80 45 30 345 300 217
Fragments arranged by size:
345 336 315 314 300 227 217 114
80 49 45 30 25 18
EarI CTCTTCn'nnn_
Cuts at: 0 535 1732 1828 2415
Size: 535 1197 96 587
Fragments arranged by size:
1197 587 535 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 357 373 654 1761 2415
Size: 357 16 281 1107 654
Fragments arranged by size:
1107 654 357 281 16
EcoICRI GAG'CTC
Cuts at: 0 456 876 2415
Size: 456 420 1539
Fragments arranged by size:
1539 456 420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 418 439 442 711 721 892 1180
Size: 169 249 21 3 269 10 171 288
Cuts at: 1180 1243 1507 1531 1756 1801 2122 2415
Size: 63 264 24 225 45 321 293
Fragments arranged by size:
321 293 288 269 264 249 225 171
169 63 45 24 21 10 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 421 448 1198 2257 2415
Size: 421 27 750 1059 158
Fragments arranged by size:
1059 750 421 158 27
EcoO109I rG'GnC_Cy
Cuts at: 0 60 61 548 675 814 847 1454 1455
Size: 60 1 487 127 139 33 607 1
Cuts at: 1455 2113 2415
Size: 658 302
Fragments arranged by size:
658 607 487 302 139 127 60 33
1 1
HaeII r_GCGC'y
Cuts at: 0 658 693 1915 1991 2415
Size: 658 35 1222 76 424
Fragments arranged by size:
1222 658 424 76 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 272 304 1368 1400 2415
Size: 272 32 1064 32 1015
Fragments arranged by size:
1064 1015 272 32 32
HindIII A'AGCT_T
Cuts at: 0 2308 2415
Size: 2308 107
KasI G'GCGC_C
Cuts at: 0 1911 1987 2415
Size: 1911 76 428
Fragments arranged by size:
1911 428 76
KpnI G_GTAC'C
Cuts at: 0 2151 2415
Size: 2151 264
MscI TGG'CCA
Cuts at: 0 247 1400 1525 1555 1900 2415
Size: 247 1153 125 30 345 515
Fragments arranged by size:
1153 515 345 247 125 30
MslI CAynn'nnrTG
Cuts at: 0 910 1072 2221 2415
Size: 910 162 1149 194
Fragments arranged by size:
1149 910 194 162
MspA1I CmG'CkG
Cuts at: 0 480 584 1248 1993 2415
Size: 480 104 664 745 422
Fragments arranged by size:
745 664 480 422 104
NaeI GCC'GGC
Cuts at: 0 340 610 1921 2079 2415
Size: 340 270 1311 158 336
Fragments arranged by size:
1311 340 336 270 158
NarI GG'CG_CC
Cuts at: 0 1912 1988 2415
Size: 1912 76 427
Fragments arranged by size:
1912 427 76
NgoMIV G'CCGG_C
Cuts at: 0 338 608 1919 2077 2415
Size: 338 270 1311 158 338
Fragments arranged by size:
1311 338 338 270 158
NspI r_CATG'y
Cuts at: 0 255 492 1017 2415
Size: 255 237 525 1398
Fragments arranged by size:
1398 525 255 237
PasI CC'CwG_GG
Cuts at: 0 43 989 2117 2135 2415
Size: 43 946 1128 18 280
Fragments arranged by size:
1128 946 280 43 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 12 465 1584 2415
Size: 12 453 1119 831
Fragments arranged by size:
1119 831 453 12
PfoI T'CCnGG_A
Cuts at: 0 113 1778 2415
Size: 113 1665 637
Fragments arranged by size:
1665 637 113
PpuMI rG'GwC_Cy
Cuts at: 0 548 2415
Size: 548 1867
PspOMI G'GGCC_C
Cuts at: 0 60 813 846 1454 2415
Size: 60 753 33 608 961
Fragments arranged by size:
961 753 608 60 33
PspXI vC'TCGA_Gb
Cuts at: 0 2031 2415
Size: 2031 384
PstI C_TGCA'G
Cuts at: 0 158 674 1534 1610 2415
Size: 158 516 860 76 805
Fragments arranged by size:
860 805 516 158 76
PvuII CAG'CTG
Cuts at: 0 1248 2415
Size: 1248 1167
SacI G_AGCT'C
Cuts at: 0 458 878 2415
Size: 458 420 1537
Fragments arranged by size:
1537 458 420
SapI GCTCTTCn'nnn_
Cuts at: 0 535 1828 2415
Size: 535 1293 587
Fragments arranged by size:
1293 587 535
SbfI CC_TGCA'GG
Cuts at: 0 674 1610 2415
Size: 674 936 805
Fragments arranged by size:
936 805 674
ScaI AGT'ACT
Cuts at: 0 301 2415
Size: 301 2114
SfcI C'TryA_G
Cuts at: 0 154 670 927 1530 1606 2415
Size: 154 516 257 603 76 809
Fragments arranged by size:
809 603 516 257 154 76
SfoI GGC'GCC
Cuts at: 0 1913 1989 2415
Size: 1913 76 426
Fragments arranged by size:
1913 426 76
SmlI C'TyrA_G
Cuts at: 0 507 754 1546 2031 2257 2415
Size: 507 247 792 485 226 158
Fragments arranged by size:
792 507 485 247 226 158
SphI G_CATG'C
Cuts at: 0 255 2415
Size: 255 2160
SspI AAT'ATT
Cuts at: 0 1209 2415
Size: 1209 1206
StuI AGG'CCT
Cuts at: 0 869 2415
Size: 869 1546
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 232 916 985 1002 2079 2127 2415
Size: 232 684 69 17 1077 48 288
Fragments arranged by size:
1077 684 288 232 69 48 17
TatI w'GTAC_w
Cuts at: 0 299 914 1076 2415
Size: 299 615 162 1339
Fragments arranged by size:
1339 615 299 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 224 256 1693 1725 2415
Size: 224 32 1437 32 690
Fragments arranged by size:
1437 690 224 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1669 2415
Size: 1669 746
XhoI C'TCGA_G
Cuts at: 0 2031 2415
Size: 2031 384
Enzymes that do cut:
AarI Acc65I AcuI AflII AgeI AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BcgI BglI
Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI Bsu36I
BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII KasI KpnI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI
PpuMI PspOMI PspXI PstI PvuII SacI SapI SbfI
ScaI SfcI SfoI SmlI SphI SspI StuI TaqII
TatI TstI Tth111I XhoI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AhdI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BfrBI BglII BlpI BmtI BplI Bpu10I
BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BssHII BssSI
BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI MfeI MluI MmeI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI
SanDI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI StyI SwaI XbaI XcmI XmaI XmnI ZraI