Restriction map for
pFN21ASDF0106
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflIII Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspHI AspI AvrII BamHI BbrPI
BciVI BclI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DsaI
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI HpaI Ksp632I
KspI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tsp509I Tth111I
TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 911 GGTACC G^GTACC
AcyI 1045 GGCGCC G[GA]^CG[CT]C
AflII 351 CTTAAG C^TTAAG
AgeI 242 ACCGGT A^CCGGT
AhdI 502 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 204 GATCC GGATC(4/5)
AlwNI 466 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 911 GGTACC G^GTACC
AspEI 502 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 893 TGCGCA TGC^GCA
BbeI 1045 GGCGCC GGCGC^C
BcgI 678 GCAGGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 422 CTAG C^TAG
BfrI 351 CTTAAG C^TTAAG
BsaHI 1045 GGCGCC G[GA]^CG[CT]C
BsaWI 242 ACCGGT [AT]^CCGG[AT]
BsgI 864 GTGCAG GTGCAG(16/14)
BsiEI 549 CGGCCG CG[GA][CT]^CG
BsmBI 257 CGTCTC CGTCTC(1/5)
BsrBI 100 GAGCGG CCGCTC(-3/-3)
BstYI 203 AGATCC [GA]^GATC[CT]
DpnII 204 GATC ^GATC
DrdI 655 GACTCGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 549 CGGCCG C^GGCCG
Eam1105I 502 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 549 CGGCCG C^GGCCG
Eco52I 549 CGGCCG C^GGCCG
FseI 540 GGCCGGCC GGCCGG^CC
FspI 893 TGCGCA TGC^GCA
Hin1I 1045 GGCGCC G[GA]^CG[CT]C
HincII 6 GTTGAC GT[CT]^[GA]AC
HindII 6 GTTGAC GT[CT]^[GA]AC
HindIII 1022 AAGCTT A^AGCTT
KasI 1045 GGCGCC G^GCGCC
KpnI 911 GGTACC GGTAC^C
MaeI 422 CTAG C^TAG
MaeII 256 ACGT A^CGT
MboI 204 GATC ^GATC
MflI 203 AGATCC [GA]^GATC[CT]
MseI 352 TTAA T^TAA
NarI 1045 GGCGCC GG^CGCC
NdeII 204 GATC ^GATC
PflMI 465 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 242 ACCGGT A^CCGGT
PpuMI 355 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 235 CAGCTG CAG^CTG
Sau3AI 204 GATC ^GATC
SfoI 1045 GGCGCC GGC^GCC
SmlI 351 CTTAAG C^T[CT][GA]AG
Tru9I 352 TTAA T^TAA
Van91I 465 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 608 CCAGGCCCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 203 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 869 GTAC GT^AC
912 GTAC
ApaI 594 GGGCCC GGGCC^C
824 GGGCCC
AvaII 240 GGACC G^G[AT]CC
356 GGTCC
BalI 149 TGGCCA TGG^CCA
830 TGGCCA
BanI 911 GGTACC G^G[CT][GA]CC
1045 GGCGCC
BbsI 1068 GAAGAC GAAGAC(2/6)
664 GTCTTC
BbvCI 381 CCTCAGC CCTCAGC(-5/-2)
751 GCTGAGG
BpuAI 1068 GAAGAC GAAGAC(2/6)
664 GTCTTC
BsaI 1077 GAGACC GGTCTC(1/5)
245 GGTCTC
BseRI 640 CTCCTC GAGGAG(10/8)
754 GAGGAG
BstXI 28 CCAGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
821 CCAGGGCCCTGG
EcoNI 347 CCTCCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
645 CCTGGACGAGG
EcoT14I 113 CCAAGG C^C[AT][AT]GG
287 CCTTGG
HaeII 30 AGCGCC [GA]GCGC^[CT]
1045 GGCGCC
HgaI 118 GACGC GACGC(5/10)
985 GACGC
HphI 84 GGTGA GGTGA(8/7)
409 TCACC
MaeIII 85 GTGAC ^GT[ACGT]AC
1014 GTCAC
MluNI 149 TGGCCA TGG^CCA
830 TGGCCA
MscI 149 TGGCCA TGG^CCA
830 TGGCCA
MspA1I 235 CAGCTG C[AC]G^C[GT]G
494 CCGCTG
NaeI 394 GCCGGC GCC^GGC
541 GCCGGC
NgoMIV 394 GCCGGC G^CCGGC
541 GCCGGC
NruI 687 TCGCGA TCG^CGA
878 TCGCGA
PstI 232 CTGCAG CTGCA^G
676 CTGCAG
RsaI 869 GTAC GT^AC
912 GTAC
SfcI 232 CTGCAG C^T[GA][CT]AG
676 CTGCAG
SmaI 483 CCCGGG CCC^GGG
591 CCCGGG
SphI 176 GCATGC GCATG^C
183 GCATGC
StyI 113 CCAAGG C^C[AT][AT]GG
287 CCTTGG
Tsp45I 85 GTGAC ^GT[GC]AC
1014 GTCAC
XmaCI 483 CCCGGG C^CCGGG
591 CCCGGG
XmaI 483 CCCGGG C^CCGGG
591 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboII MnlI MslI
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV NspI PleI Sau96I
ScrFI SfaNI TseI TspRI