(Circular) (Six-base) MAPSORT of: pFN21ASDF0106.seq Check: 9421 from: 1 to: 5827 pFN21ASDF0106 5827 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3637 278 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 2934 3148 2934 Size: 214 5613 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3002 3890 5274 5542 1632 Size: 1370 888 1384 268 1917 Fragments arranged by size: 1917 1384 1370 888 268 AflII C'TTAA_G Cuts at: 820 1017 2374 820 Size: 197 1357 4273 Fragments arranged by size: 4273 1357 197 AflIII A'CryG_T Cuts at: 1203 4726 5703 1203 Size: 3523 977 1327 Fragments arranged by size: 3523 1327 977 AgeI A'CCGG_T Cuts at: 2265 4658 2265 Size: 2393 3434 AhdI GACnn_n'nnGTC Cuts at: 2530 4556 2530 Size: 2026 3801 AleI CACnn'nnGTG Cuts at: 1166 2877 1166 Size: 1711 4116 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5827 AlwNI CAG_nnn'CTG Cuts at: 2494 3570 5142 2494 Size: 1076 1572 3179 Fragments arranged by size: 3179 1572 1076 ApaI G_GGCC'C Cuts at: 2621 2851 2621 Size: 230 5597 ApaLI G'TGCA_C Cuts at: 3884 5040 3884 Size: 1156 4671 ApoI r'AATT_y Cuts at: 1493 3136 3362 3514 5623 1493 Size: 1643 226 152 2109 1697 Fragments arranged by size: 2109 1697 1643 226 152 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 2410 2506 2614 3152 1870 Size: 73 467 96 108 538 4545 Fragments arranged by size: 4545 538 467 108 96 73 BamHI G'GATC_C Cuts at: 3157 3524 3157 Size: 367 5460 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2934 3068 3148 4603 618 Size: 325 217 627 1147 134 80 1455 1842 Fragments arranged by size: 1842 1455 1147 627 325 217 134 80 BanII G_rGCy'C Cuts at: 1391 2240 2342 2621 2822 2851 3146 1391 Size: 849 102 279 201 29 295 4072 Fragments arranged by size: 4072 849 295 279 201 102 29 BbeI G_GCGC'C Cuts at: 3072 3072 Size: 5827 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2680 3098 928 Size: 971 781 418 3657 Fragments arranged by size: 3657 971 781 418 BbvCI CC'TCA_GC Cuts at: 2405 2775 2405 Size: 370 5457 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2690 2724 4018 4052 1903 Size: 34 753 34 1294 34 3678 Fragments arranged by size: 3678 1294 753 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3719 4929 3719 Size: 1210 4617 BclI T'GATC_A Cuts at: 1548 1548 Size: 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2251 2580 2771 Size: 107 122 71 631 605 579 329 191 Cuts at: 2771 2846 2946 4438 136 Size: 75 100 1492 1525 Fragments arranged by size: 1525 1492 631 605 579 329 191 122 107 100 75 71 BglII A'GATC_T Cuts at: 5822 5822 Size: 5827 BlpI GC'TnA_GC Cuts at: 3244 3244 Size: 5827 Bme1580I G_kGCm'C Cuts at: 1790 2621 2851 2997 3888 5044 1790 Size: 831 230 146 891 1156 2573 Fragments arranged by size: 2573 1156 891 831 230 146 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5827 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2818 3558 4516 Size: 416 53 83 399 24 1401 740 958 Cuts at: 4516 442 Size: 1753 Fragments arranged by size: 1753 1401 958 740 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2178 2268 2374 2481 2614 2789 2827 4487 Size: 939 90 106 107 133 175 38 1660 Cuts at: 4487 1239 Size: 2579 Fragments arranged by size: 2579 1660 939 175 133 107 106 90 38 Bpu10I CC'TnA_GC Cuts at: 2405 2775 2405 Size: 370 5457 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3952 4817 5115 3952 Size: 865 298 4664 Fragments arranged by size: 4664 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2274 3094 4490 882 Size: 617 775 820 1396 2219 Fragments arranged by size: 2219 1396 820 775 617 BsaAI yAC'GTr Cuts at: 493 1204 3124 3618 493 Size: 711 1920 494 2702 Fragments arranged by size: 2702 1920 711 494 BsaBI GATnn'nnATC Cuts at: 5821 5821 Size: 5827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3069 3634 4016 4651 Size: 53 83 186 936 1536 565 382 635 Cuts at: 4651 275 Size: 1451 Fragments arranged by size: 1536 1451 936 635 565 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2265 4260 4658 4932 5079 5490 2265 Size: 1995 398 274 147 411 2602 Fragments arranged by size: 2602 1995 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2918 2948 1178 Size: 30 165 30 1515 30 4057 Fragments arranged by size: 4057 1515 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2654 2792 1186 Size: 1468 138 4221 Fragments arranged by size: 4221 1468 138 BseYI C'CCAG_C Cuts at: 2636 2807 5030 5526 2636 Size: 171 2223 496 2937 Fragments arranged by size: 2937 2223 496 171 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2908 1163 Size: 573 1172 4082 Fragments arranged by size: 4082 1172 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2575 3277 4038 5066 1540 Size: 275 186 574 702 761 1028 2301 Fragments arranged by size: 2301 1028 761 702 574 275 186 BsiHKAI G_wGCw'C Cuts at: 3888 3973 5044 3888 Size: 85 1071 4671 Fragments arranged by size: 4671 1071 85 BsmI GAATG_Cn' Cuts at: 3338 3431 3338 Size: 93 5734 BsmBI CGTCTCn'nnnn_ Cuts at: 2286 2286 Size: 5827 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2342 2621 2822 2851 2997 3146 Size: 399 450 102 279 201 29 146 149 Cuts at: 3146 3888 3973 5044 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 450 399 279 201 149 146 102 85 29 BspEI T'CCGG_A Cuts at: 5490 5490 Size: 5827 BspHI T'CATG_A Cuts at: 3716 5446 3716 Size: 1730 4097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3182 844 Size: 2338 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2125 3714 1394 Size: 429 302 1589 3507 Fragments arranged by size: 3507 1589 429 302 BsrDI GCAATG_nn' Cuts at: 65 1248 4322 4496 65 Size: 1183 3074 174 1396 Fragments arranged by size: 3074 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2265 2417 2564 2931 4471 4658 1700 Size: 110 455 152 147 367 1540 187 2869 Fragments arranged by size: 2869 1540 455 367 187 152 147 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5827 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5827 BssSI C'ACGA_G Cuts at: 3887 4899 3887 Size: 1012 4815 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5827 BstBI TT'CG_AA Cuts at: 2007 4631 2007 Size: 2624 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5827 BstXI CCAn_nnnn'nTGG Cuts at: 2058 2851 2058 Size: 793 5034 BstYI r'GATC_y Cuts at: 1972 2226 3157 3524 3909 3926 5378 5464 5476 Size: 254 931 367 385 17 1452 86 12 Cuts at: 5476 5822 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 931 385 367 346 254 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4318 Fragments arranged by size: 4318 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2884 487 Size: 683 56 1658 3430 Fragments arranged by size: 3430 1658 683 56 BtsI GCAGTG_nn' Cuts at: 3343 4137 4157 3343 Size: 794 20 5013 Fragments arranged by size: 5013 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5792 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2684 4834 809 Size: 507 1368 2150 1802 Fragments arranged by size: 2150 1802 1368 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2118 2172 Size: 54 1425 50 275 18 213 75 54 Cuts at: 2172 2469 2572 2853 3274 4163 5792 8 Size: 297 103 281 421 889 1629 43 Fragments arranged by size: 1629 1425 889 421 297 281 275 213 103 75 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2572 3274 1537 Size: 275 760 702 4090 Fragments arranged by size: 4090 760 702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3757 1353 Size: 2404 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2219 4414 4788 4934 387 Size: 1064 138 630 2195 374 146 1280 Fragments arranged by size: 2195 1280 1064 630 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2178 2268 2374 2481 2614 2789 2827 Size: 393 546 90 106 107 133 175 38 Cuts at: 2827 3002 3890 4487 5274 5542 1239 Size: 175 888 597 787 268 1524 Fragments arranged by size: 1524 888 787 597 546 393 268 175 175 133 107 106 90 38 EcoNI CCTnn'n_nnAGG Cuts at: 2374 2672 2374 Size: 298 5529 EcoO109I rG'GnC_Cy Cuts at: 2379 2618 2847 2848 2379 Size: 239 229 1 5358 Fragments arranged by size: 5358 239 229 1 EcoRI G'AATT_C Cuts at: 3136 3514 3136 Size: 378 5449 FseI GG_CCGG'CC Cuts at: 2568 2568 Size: 5827 FspI TGC'GCA Cuts at: 2918 4333 2918 Size: 1415 4412 FspAI rTGC'GCAy Cuts at: 2918 2918 Size: 5827 HaeII r_GCGC'y Cuts at: 1678 2057 3072 4974 1678 Size: 379 1015 1902 2531 Fragments arranged by size: 2531 1902 1015 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2151 2183 2351 2383 2516 Size: 32 135 32 579 32 168 32 133 Cuts at: 2516 2548 2663 2695 4468 4500 4542 4574 1373 Size: 32 115 32 1773 32 42 32 2626 Fragments arranged by size: 2626 1773 579 168 135 133 115 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2031 3171 3417 669 Size: 988 281 93 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 93 HindIII A'AGCT_T Cuts at: 748 3045 748 Size: 2297 3530 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KasI G'GCGC_C Cuts at: 3068 3068 Size: 5827 KpnI G_GTAC'C Cuts at: 2938 3152 2938 Size: 214 5613 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4941 5125 1305 Size: 3636 184 2007 Fragments arranged by size: 3636 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 2855 10 Size: 54 1425 343 342 681 2982 Fragments arranged by size: 2982 1425 681 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2105 2168 2877 3785 4144 4303 518 Size: 648 939 63 709 908 359 159 2042 Fragments arranged by size: 2042 939 908 709 648 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2260 2519 3243 3920 5068 5313 1606 Size: 654 259 724 677 1148 245 2120 Fragments arranged by size: 2120 1148 724 677 654 259 245 NaeI GCC'GGC Cuts at: 2419 2566 2419 Size: 147 5680 NarI GG'CG_CC Cuts at: 3069 3069 Size: 5827 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4318 Fragments arranged by size: 4318 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NgoMIV G'CCGG_C Cuts at: 2417 2564 2417 Size: 147 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NruI TCG'CGA Cuts at: 2712 2903 2712 Size: 191 5636 NspI r_CATG'y Cuts at: 1132 1229 2203 2210 2502 3185 4730 1132 Size: 97 974 7 292 683 1545 2229 Fragments arranged by size: 2229 1545 974 683 292 97 7 PasI CC'CwG_GG Cuts at: 1348 2058 1348 Size: 710 5117 PciI A'CATG_T Cuts at: 4726 4726 Size: 5827 PflMI CCAn_nnn'nTGG Cuts at: 1108 2494 1108 Size: 1386 4441 PfoI T'CCnGG_A Cuts at: 2667 2922 2667 Size: 255 5572 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2379 2379 Size: 5827 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5827 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PspOMI G'GGCC_C Cuts at: 2617 2847 2617 Size: 230 5597 PstI C_TGCA'G Cuts at: 830 2259 2703 3179 830 Size: 1429 444 476 3478 Fragments arranged by size: 3478 1429 476 444 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 PvuII CAG'CTG Cuts at: 2260 2260 Size: 5827 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SbfI CC_TGCA'GG Cuts at: 3179 3179 Size: 5827 ScaI AGT'ACT Cuts at: 4075 5787 4075 Size: 1712 4115 SfcI C'TryA_G Cuts at: 826 1045 2255 2699 3175 4310 4991 5182 826 Size: 219 1210 444 476 1135 681 191 1471 Fragments arranged by size: 1471 1210 1135 681 476 444 219 191 SfoI GGC'GCC Cuts at: 3070 3070 Size: 5827 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5827 SmaI CCC'GGG Cuts at: 1872 2508 2616 3154 1872 Size: 636 108 538 4545 Fragments arranged by size: 4545 636 538 108 SmlI C'TyrA_G Cuts at: 820 1017 1943 2374 3931 4832 5094 820 Size: 197 926 431 1557 901 262 1553 Fragments arranged by size: 1557 1553 926 901 431 262 197 SnaBI TAC'GTA Cuts at: 493 3124 3618 493 Size: 2631 494 2702 Fragments arranged by size: 2702 2631 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 2203 2210 3185 1132 Size: 1071 7 975 3774 Fragments arranged by size: 3774 1071 975 7 SspI AAT'ATT Cuts at: 5 52 3751 5 Size: 47 3699 2081 Fragments arranged by size: 3699 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5827 StyI C'CwwG_G Cuts at: 513 1065 2022 2136 2310 3266 513 Size: 552 957 114 174 956 3074 Fragments arranged by size: 3074 957 956 552 174 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3854 3871 4024 4209 4654 325 Size: 1255 2274 17 153 185 445 1498 Fragments arranged by size: 2274 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4073 5785 96 Size: 274 80 33 51 1443 2096 1712 138 Fragments arranged by size: 2096 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5787 5819 5787 Size: 32 5795 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5827 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2638 2638 Size: 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 2506 2614 3152 1870 Size: 636 108 538 4545 Fragments arranged by size: 4545 636 538 108 XmnI GAAnn'nnTTC Cuts at: 3956 3956 Size: 5827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3635 276 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI