Restriction map for
pFN21ASDF0106
(Linear) (Six-base) MAPSORT of: FLJ00106.seq Check: 66 from: 1 to: 1098
FLJ00106.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
Acc65I G'GTAC_C
Cuts at: 0 911 1098
Size: 911 187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1098
Size: 979 119
AflII C'TTAA_G
Cuts at: 0 351 1098
Size: 351 747
AgeI A'CCGG_T
Cuts at: 0 242 1098
Size: 242 856
AhdI GACnn_n'nnGTC
Cuts at: 0 507 1098
Size: 507 591
AleI CACnn'nnGTG
Cuts at: 0 854 1098
Size: 854 244
AlwNI CAG_nnn'CTG
Cuts at: 0 471 1098
Size: 471 627
ApaI G_GGCC'C
Cuts at: 0 598 828 1098
Size: 598 230 270
Fragments arranged by size:
598 270 230
AvaI C'yCGr_G
Cuts at: 0 387 483 591 1098
Size: 387 96 108 507
Fragments arranged by size:
507 387 108 96
BanI G'GyrC_C
Cuts at: 0 911 1045 1098
Size: 911 134 53
Fragments arranged by size:
911 134 53
BanII G_rGCy'C
Cuts at: 0 217 319 598 799 828 1098
Size: 217 102 279 201 29 270
Fragments arranged by size:
279 270 217 201 102 29
BbeI G_GCGC'C
Cuts at: 0 1049 1098
Size: 1049 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 657 1075 1098
Size: 657 418 23
Fragments arranged by size:
657 418 23
BbvCI CC'TCA_GC
Cuts at: 0 382 752 1098
Size: 382 370 346
Fragments arranged by size:
382 370 346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 667 701 1098
Size: 667 34 397
Fragments arranged by size:
667 397 34
BglI GCCn_nnn'nGGC
Cuts at: 0 228 557 748 823 923 1098
Size: 228 329 191 75 100 175
Fragments arranged by size:
329 228 191 175 100 75
Bme1580I G_kGCm'C
Cuts at: 0 598 828 974 1098
Size: 598 230 146 124
Fragments arranged by size:
598 230 146 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 795 1098
Size: 795 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 245 351 458 591 766 804 1098
Size: 155 90 106 107 133 175 38 294
Fragments arranged by size:
294 175 155 133 107 106 90 38
Bpu10I CC'TnA_GC
Cuts at: 0 382 752 1098
Size: 382 370 346
Fragments arranged by size:
382 370 346
BsaI GGTCTCn'nnnn_
Cuts at: 0 251 1071 1098
Size: 251 820 27
Fragments arranged by size:
820 251 27
BsaHI Gr'CG_yC
Cuts at: 0 1046 1098
Size: 1046 52
BsaWI w'CCGG_w
Cuts at: 0 242 1098
Size: 242 856
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 1098
Size: 895 30 173
Fragments arranged by size:
895 173 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 631 769 1098
Size: 631 138 329
Fragments arranged by size:
631 329 138
BseYI C'CCAG_C
Cuts at: 0 613 784 1098
Size: 613 171 314
Fragments arranged by size:
613 314 171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 885 1098
Size: 885 213
BsiEI CG_ry'CG
Cuts at: 0 552 1098
Size: 552 546
BsmBI CGTCTCn'nnnn_
Cuts at: 0 263 1098
Size: 263 835
Bsp1286I G_dGCh'C
Cuts at: 0 217 319 598 799 828 974 1098
Size: 217 102 279 201 29 146 124
Fragments arranged by size:
279 217 201 146 124 102 29
BsrBI CCG'CTC
Cuts at: 0 102 1098
Size: 102 996
BsrFI r'CCGG_y
Cuts at: 0 242 394 541 908 1098
Size: 242 152 147 367 190
Fragments arranged by size:
367 242 190 152 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 35 828 1098
Size: 35 793 270
Fragments arranged by size:
793 270 35
BstYI r'GATC_y
Cuts at: 0 203 1098
Size: 203 895
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 861 1098
Size: 861 237
DrdI GACnn_nn'nnGTC
Cuts at: 0 661 1098
Size: 661 437
EaeI y'GGCC_r
Cuts at: 0 20 95 149 446 549 830 1098
Size: 20 75 54 297 103 281 268
Fragments arranged by size:
297 281 268 103 75 54 20
EagI C'GGCC_G
Cuts at: 0 549 1098
Size: 549 549
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 196 1098
Size: 196 902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 245 351 458 591 766 804 979
Size: 155 90 106 107 133 175 38 175
Cuts at: 979 1098
Size: 119
Fragments arranged by size:
175 175 155 133 119 107 106 90
38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 351 649 1098
Size: 351 298 449
Fragments arranged by size:
449 351 298
EcoO109I rG'GnC_Cy
Cuts at: 0 356 595 824 825 1098
Size: 356 239 229 1 273
Fragments arranged by size:
356 273 239 229 1
FseI GG_CCGG'CC
Cuts at: 0 545 1098
Size: 545 553
FspI TGC'GCA
Cuts at: 0 895 1098
Size: 895 203
FspAI rTGC'GCAy
Cuts at: 0 895 1098
Size: 895 203
HaeII r_GCGC'y
Cuts at: 0 34 1049 1098
Size: 34 1015 49
Fragments arranged by size:
1015 49 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 128 160 328 360 493 525 640 672
Size: 128 32 168 32 133 32 115 32
Cuts at: 672 1098
Size: 426
Fragments arranged by size:
426 168 133 128 115 32 32 32
32
HincII GTy'rAC
Cuts at: 0 8 1098
Size: 8 1090
HindIII A'AGCT_T
Cuts at: 0 1022 1098
Size: 1022 76
KasI G'GCGC_C
Cuts at: 0 1045 1098
Size: 1045 53
KpnI G_GTAC'C
Cuts at: 0 915 1098
Size: 915 183
MscI TGG'CCA
Cuts at: 0 151 832 1098
Size: 151 681 266
Fragments arranged by size:
681 266 151
MslI CAynn'nnrTG
Cuts at: 0 82 145 854 1098
Size: 82 63 709 244
Fragments arranged by size:
709 244 82 63
MspA1I CmG'CkG
Cuts at: 0 237 496 1098
Size: 237 259 602
Fragments arranged by size:
602 259 237
NaeI GCC'GGC
Cuts at: 0 396 543 1098
Size: 396 147 555
Fragments arranged by size:
555 396 147
NarI GG'CG_CC
Cuts at: 0 1046 1098
Size: 1046 52
NgoMIV G'CCGG_C
Cuts at: 0 394 541 1098
Size: 394 147 557
Fragments arranged by size:
557 394 147
NruI TCG'CGA
Cuts at: 0 689 880 1098
Size: 689 191 218
Fragments arranged by size:
689 218 191
NspI r_CATG'y
Cuts at: 0 180 187 479 1098
Size: 180 7 292 619
Fragments arranged by size:
619 292 180 7
PasI CC'CwG_GG
Cuts at: 0 35 1098
Size: 35 1063
PflMI CCAn_nnn'nTGG
Cuts at: 0 471 1098
Size: 471 627
PfoI T'CCnGG_A
Cuts at: 0 644 899 1098
Size: 644 255 199
Fragments arranged by size:
644 255 199
PpuMI rG'GwC_Cy
Cuts at: 0 356 1098
Size: 356 742
PspOMI G'GGCC_C
Cuts at: 0 594 824 1098
Size: 594 230 274
Fragments arranged by size:
594 274 230
PstI C_TGCA'G
Cuts at: 0 236 680 1098
Size: 236 444 418
Fragments arranged by size:
444 418 236
PvuII CAG'CTG
Cuts at: 0 237 1098
Size: 237 861
SfcI C'TryA_G
Cuts at: 0 232 676 1098
Size: 232 444 422
Fragments arranged by size:
444 422 232
SfoI GGC'GCC
Cuts at: 0 1047 1098
Size: 1047 51
SmaI CCC'GGG
Cuts at: 0 485 593 1098
Size: 485 108 505
Fragments arranged by size:
505 485 108
SmlI C'TyrA_G
Cuts at: 0 351 1098
Size: 351 747
SphI G_CATG'C
Cuts at: 0 180 187 1098
Size: 180 7 911
Fragments arranged by size:
911 180 7
StyI C'CwwG_G
Cuts at: 0 113 287 1098
Size: 113 174 811
Fragments arranged by size:
811 174 113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 615 1098
Size: 615 483
XmaI C'CCGG_G
Cuts at: 0 483 591 1098
Size: 483 108 507
Fragments arranged by size:
507 483 108
Enzymes that do cut:
Acc65I AcuI AflII AgeI AhdI AleI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI
Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BsrBI BsrFI
BstXI BstYI BtgZI DrdI EaeI EagI EciI Eco57MI
EcoNI EcoO109I FseI FspI FspAI HaeII Hin4I HincII
HindIII KasI KpnI MscI MslI MspA1I NaeI NarI
NgoMIV NruI NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SfcI SfoI SmaI SmlI SphI StyI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsiHKAI BsiWI BsmI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII
EarI EcoICRI EcoRI EcoRV FalI HpaI MfeI MluI
MmeI NcoI NdeI NheI NotI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmnI ZraI